一地一牡丹

正"国色朝酣酒,天香夜染衣。丹景春醉容,明月问归期。"牡丹为我国特有植物,自唐朝以来就深受我国人民的喜爱,她在我国古代人民心中的地位有如长江、黄河,可仅用一个"花"字来代表。"牡丹"既特指栽培供观赏的各种品种,又泛指芍药科(Paeoniaceae)芍药属(Paeonia?L.)牡丹组(sect.?Moutan?Stern)的所有物种。有确切文字记载的牡丹栽培始于武则天,不同野生牡丹物     

一年又一年

上了年纪的人都有相似的感觉,就是年纪越大,时间好像过得越快。小时候,过了元旦盼春节,过了春节盼五一、六一,过了六一盼国庆,过了国庆再盼元旦。时间总是那么漫长,盼望的假期似乎总是姗姗来迟。而如今,似乎一眨眼,一年就过去了。我给这个现象总结了一个理论,叫做相对     

罕见的Rh缺失型一D一／一D一例报告

-D-/-D一型是Rh 系血型的稀有型之一， 1950年最初由Race等报道^[9,12]。-D-/-D- （瓦瓦）型在世界上已发现有20余例^[1,3]，此中 日本人占8例之多，而我国尚未见有一例报道。 最近我们在检查一例新生儿溶血病患者血清时 遇到异常抗体，追查其母亲血，始终未能检出 C, c, E, e抗原，而只能证明强力的D抗原，即 -D-/-D一型。同时在其血清中证明了抗CcEe, 即抗Hro抗体。     

一滴海水一碗汤

正咕噜博士不做实验改做饭,最新推出一道"生猛海鲜汤"。其中富含蛋白质及多种维生素,能为你和家人提供完美均衡的营养,只售9.9元,现已列入"博士牌"豪华午餐!还等什么,快打电话订购吧!和大伙儿开个玩笑,咕噜博士的厨艺其实可以忽略不计,他的"海鲜汤"里有什么也只有借助显微镜才能看清。现在咱们就取一滴拿到显微镜下观察一番,看这"汤"里都有哪些"猛料"吧!     

一沙一海一世界

<正>国家鼓励单位和个人在自愿的前提下,捐资或者以其他形式参与海洋特别保护区建设与管理。海洋特别保护区内的经营性开发利用活动,可以依照有关法律法规和海洋特别保护区管理制度及总体规划,由海洋特别保护区管理机构实施,也可以在海洋特别保护区管理机构监管下,采用公开招标方式授权企业经营。授权企业经营的,海洋特别保护区管理机构应当与企业签订特许经营协议,实行资源有     

一方酒养一方人

葡萄酒不过是一种由葡萄发酵而成的、含有酒精成分的饮料,但是各种葡萄酒却有着那么大的不同。为什么一款普通的国产酒只能卖30多元人民币,一瓶波尔多一等酒庄的产品却可以卖到200美元,而一些稀有的勃艮第酒一上市则可以达到2000欧元的高价?为什么有些葡萄酒越放越便宜,甚至要到挥泪大甩卖的地步,而有的则越放越金贵?     

开篇:一馆一省看世界

正走进山西地质博物馆,就如同展开了山西地质的科学画卷,在这里,您不仅可以感受到大自然的鬼斧神工,了解山西的地质发展历史,还可以穿越古老的地球时空,触摸到地球的脉动,目睹古生物的起源和演变历程,领略丰富多彩的地学知识与地质文化。沧海桑田,芸芸众生,林林总总,无数生物经历了从无到有,从简单到复杂的起源、发展、演化进程。古生物化石既是地球生命演化的真实记载和远古历史的客观见证,又是大自然遗存的珍     

一本书与一种教育思想

在一本高校基础教材的书名中出现人名,在国内即使不是第一次,也是非常罕见的。究竟这样做的用意何在?我想,这首先体现了编者对此人的尊敬与怀念,但原因却并不仅限于此。刚刚由高等教育出版社在2005年1月出版的《陈阅增普通生物学》是在陈阅增先生主编的《普通生物学》(1997年出版)基础上经过修订后完成的。陈阅增先生是我国著名的生物学家,曾任中国动物学会理事长、教育部理科生物     

序一

著名菌物学家余永年教授,派名业丰,号南山、功懋,字永年,笔名南丰,重庆市万州人氏,于1923年4月8日生于书香门第中医世家.今年正逢教授九十大寿之际,使我暮然回首教授而立之年,即1957年3月,他被录取为中国科学院应用真菌学研究所所长,中国科学院学部委员,即中国科学院院士前称谓,中国真菌学奠基人戴芳澜教授研究生时的青年时代,犹如昨日,历历在目.     

从一粒花粉到一棵植株

花粉单倍体育种中,花药离体培养能否成功的关键之一是花粉的年龄。自然条件下花粉最初只有一个核,称为单核花粉,以后经过一次有丝分裂产生一个营养核和一个生殖核,称为两核花粉。营养核不再分裂,生殖核再经一次有丝分裂成两个精子,称为三核花粉。诱导单倍体最好用单核中晚期(单核靠近质膜时)的花粉。取下合适的花蕾或幼穗先经消毒灭菌,再在无菌条件下取出花药,接种到培养基上,培养条件对花粉的发育有十分明显的影响,一般进行花药培养的温度约在23—28℃之间,需给于适当的光照。由花粉长成单倍体植株一般有两条途径:一是由花粉分裂形成愈伤组织,再将愈伤组     

A homozygous female hemophilia A

BACKGROUND:

Hemophilia A (HA), being an X-linked recessive disorder, females are rarely affected, although they can be carriers.

AIMS:

To study the mutation in F8 gene in an extended family with a homozygous female HA.

MATERIALS AND METHODS:

All the seven affected members (six males and one female) were initially screened by Conformation Sensitive Gel Electrophoresis (CSGE) and direct DNA sequencing.

RESULTS:

A homozygous missense mutation c.1315G>A (p.Gly420Ser) was identified in exon 9 of F8 gene in homozygous state in the affected female born of 1° consanguinous marriage and in all the affected male members of the family. Her factor VIII levels was found to be 5.5%, vWF:Ag 120%.

CONCLUSION:

In India, as consanguineous marriages are very common in certain communities (up to 30%), the likelihood of encountering female hemophilia is higher, although this is the first case of HA out of 1600 hemophilia families registered in our Comprehensive Haemophilia Care Center. Genetic diagnosis in such cases is not necessary as all the male children will be affected and daughters obligatory carriers.     

Aminopeptidase A is angiotensinase A

Summary Quantitative histochemical measurements of aminopeptidase A (APA; E.C.3.4.11.7) were done kinetically in the kidney glomeruli of rat and mouse with an instrumental setup consisting of a microdensitometer and a computer-supported morphometric system. The histochemical demonstration of APA was carried out using the simultaneous azo coupling technique (purest-grade Fast Blue B as coupling agent and -l-glutamic acid-4-methoxy-2-naphthylamide as substrate). The methodological studies show that APA activity is calcium-ion-dependent and increases linearly with the thickness of the tissue section (3–12 m) and that the time-course of APA activity as determined by linear regression is linear only for the first 1 to 2 min of the reaction. — Kinetic measurements indicate a 40% decrease in APA activities when -l-glutamic acid-4-methoxy-2-naphthylamide (-l-Glu-MNA) is replaced by -l-aspartic acid-4-methoxy-2-naphthylamide. When -l-Glu-MNA is replaced with l-alanine-4-methoxy-2-naphthylamide, which is a substrate of aminopeptidase M (APM) only very low reaction rates are measurable (about 1.4% of those with -l-Glu-MNA). 100 and 130 mM NaCl in the incubation medium increase APA activities by approximately 16%–17%. — To clarify the functional importance of APA in the kidney, their activities were measured under the influence of angiotensins. The glomerulus was selected as the measuring site, for besides APA it contains no APM or other peptidases that could degrade angiotensins (the glomerular dipeptidyl peptidase IV is not inhibited by angiotensin II). Using the Lineweaver-Burk plot, we determined a K _m of 0.16 mM for the APA in rat glomeruli and 0.14 mM in mouse glomeruli. The V _max in mouse glomeruli is 1.6 times higher than in rat glomeruli. Ang iotensin I, II and III competitively inhibit APA in the rat and mouse glomeruli. — With quantitative histochemical techniques it was possible to show that APA is equivalent to angiotensinase A (splitting off the N-terminal aspartic acid from angiotensin I and II).Supported by the Deutsche Forschungsgemeinschaft (SFB 105)     

A glucoside of urospermal A

The aerial parts of Urospermum picroides afforded, in addition to urospermal A a p-hydroxylphenyl acetate of a glucoside of urospermal A.     

Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A,G:G:A:A,and A:A:A:A tetrads

Replacement of two to four guanines by adenines in the human telomere DNA repeat dG₃(TTAG₃)₃ did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na⁺ and K⁺ solutions. Thermodynamic data showed that, in Na⁺ solution, the dG₃(TTAG₃)₃ quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K⁺ solution, the highest destabilization was caused by the 4A tetrad. In K⁺, only the unmodified dG₃(TTAG₃)₃ quadruplex rearranged into a K⁺‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.     

A monoclonal antibody to ochratoxin A

The production of patulin by P. expansum Link ex Gray growing in apple juice was determined over a range of pH. Patulin was found to be produced in large quantities over a narrow range of pH from 3-2 to 3–8. The biomass produced increased with increasing pH. The changes in pH due to growth of the organism were found to be small.