Effects of 6-Benzylaminopurine and Potassium on {delta}-Aminolevulinate Dehydratase Activity of Excised Cucumber Cotyledons

Changes in -aminolevulinate dehydratase (ALAD) activity werefollowed in 7-day excised cotyledons maintained on water ortreated with 6-benzylaminopurine (BA 1 µg.ml^–1),KCl (10 mM) or a combination of BA + KCl. Cotyledons were incubatedfor 3 days in the dark or in the light, or preincubated for18 h in the dark before illumination. Cycloheximide treatments(10µg.ml^–1) were performed to differenciate betweenALAD biosynthesis or activation. The excision entailed an increase in ALAD activity. Then a decreaseappeared after a 24-h incubation on water in the dark. It wasmore precocious in the light, but was delayed when the cotyledonswere illuminated after a dark preincubation. BA had little effect on ALAD activity in the dark. In the light,BA completely prevented the decrease in ALAD activity observedin the control, and enhanced this activity when a dark preincubationpreceded illumination. KCl had a slight stimulating effect inthe dark, but was uneffective in the light. The combinationBA + KCl was always devoid of stimulating effect. Stimulatingeffects of BA or KCl seemed to be due to an increase in ALADbiosynthesis. No treatment markedly affected ALAD exportationfrom cytoplasm to plastids. These results are discussed in relation with cotyledon greening. (Received November 25, 1986; Accepted February 5, 1987)     

低温与光对黄瓜幼苗子叶光合电子传递活性的影响

25～30℃和30 μmol m~(-2)s~(-1)光下培养的黄瓜幼苗,在黑暗下经 1～7℃处理24h或5℃处理24～72h,光合电子传递活性受不同程度的抑制;其抑制部位主要在PSⅡ氧化侧;随温度的降低和时间的延长,抑制部位可发展至PSⅡ及之后的电子递体上,但尚未影响PSⅠ的活性。160μmol m~(-2)s~(-1)的光强加重低温对电子传递活性的抑制,光强越高,则加重的程度越高;抑制部位从PSⅡ氧化侧发展至PSⅡ反应中心以及PSⅠ。     

The Yellowing of Attached and Detached Cucumber Cotyledons

Measurements of various constituents and the activities of variousenzymes have been made in attached and detached cotyledons ofCucumis sativus. The complex changes observed suggest that thereis a reproducible pattern. Comparison of results for attachedand detached material shows that, although both become yellow,the other biochemical changes associated with yellowing arenot the same.     

5-Aminolevulinic Acid Induced Photodynamic Damage of the Photosynthetic Electron Transport Chain of Cucumber (Cucumis sativus L.) Cotyledons

Cucumber (Cucumis sativus L. cv Poinsette) plants were sprayed with 20 millimolar 5-aminolevulinic acid (ALA) and then incubated in dark for 14 hours. Upon transfer to sunlight (800 watts per square meter) the plants died after 5 hours of exposure due to photosensitization reaction of metalloporphyrins. Due to the photodynamic damage, photosystem II (PSII), photosystem I (PSI), and whole chain reactions were impaired. PSII activity was more susceptible to photodynamic damage than PSI. The variable fluorescence was significantly reduced in ALA-treated plants within 1 hour of exposure to sunlight. At low temperature (77°K), the PSI fluorescence peak height (F₇₃₄) was drastically reduced and blue shifted by 6 nanometers. The photodynamic damage was irreversible; rather, it continued upon dark incubation of ALA-treated cucumber plants exposed to sunlight for 15 minutes. In the latter experiment, continued production of malondialdehyde during dark treatment suggests the degradation of unsaturated membrane lipids.     

Effects of Lipoxygenase on the Activities of Photosynthetic Electron Transport in Cucumber Leaves

Photosynthesis and electron transport activity decreased with leaf aging, and however, lipoxygenase (Lox) activity increased correspondingly. Soybean Lox-1 inhibited significantly PSⅡ electron transport activity of chloroplasts isolated from cucumber (Cucumis sativus L. ) cotyledon. But the inhibition could be eliminated by the addition of propyl gallate (PG) or 3, 3, 4, 5, 7-pentahydroxyflavon (PF). The inhibition of PSⅠ activity by soybean Lox-1 was enhanced in the presence of 3, 4, dichlorophenyl-1, 1-dimethylurea (DCMU) or 2, 5-dibromothymoquinone (DBMIB), bfft could be restored to its original level when PG was added. Addition of 2, 2-diphenylcarbonic dihydrazide (DPC) to the mixture of isolated chloroplasts and Lox-1, PSⅡ activity resumed obviously. Chlorophyll a fluorescence study showed that Fm was decreased by Lox-1 and resumed slightly by DPC. Based on the above results, it was suggested that Lox might act at least on three sensitive sites located on Q, PQ and the oxidative side of PSⅠ . The bleaching of chlorophyll and carotenoid stimulated by Lox-l, and the inhibition of PSⅠ electron transport activity by active oxygen might be. one of the important reasons to explaine the effect of Lox on the function of photosynthetic membrane.     

Effect of Sink-Source Manipulations on the Photosynthetic Rate and Carbohydrate Content of Cucumber Cotyledons

Mayoral, M. L., Plaut, Z. and Reinhold, L. 1985. Effect of sink-sourcemanipulations on the photosynthetic rate and carbohydrate contentof cucumber cotyledons.-J. exp. Bot. 36 1551–1558. The photosynthetic rate of cucumber cotyledons (Cucumis sativuscv. Dahla) reached a maximum value of 12 mg dm^–2 h^–1,10 d after emergence. In 12-d-old seedlings removal of one cotyledondoubled the CO₂ fixation rate of the other, as observed 3 dafter treatment. When the primary leaf was removed, the photosyntheticrate of the cotyledons was decreased by 33%. At this stage ofgrowth elimination of the roots as a sink for assimilates bygirdling the hypocotyl affected neither the photosynthetic ratenor the carbohydrate content of the cotyledons. By contrast,in 18-d-old seedlings removal of the first leaf brought abouta 42% increase in the photosynthetic rate of the cotyledons.The simultaneous removal of the first leaf and one cotyledondoubled the rate of CO₂ fixation of the remaining cotyledon.Girdling the hypocotyl lowered the photosynthetic rate of thecotyledons by 73%. In both 12- and 18-d-old seedlings a decreaseor increase in the sink-source ratio was correlated with anincrease or a decrease respectively in the carbohydrate contentof the cotyledons. The stomatal resistance of the cotyledonswas not affected by any of the treatments. The effect of sink-sourcemanipulations on photosynthesis and on the level of carbohydratespresent in the cotyledons was more evident in those seedlingsgrowing under high light intensity (580 µE m^–2 s^–1),than in those exposed to 300 µE m^–2 s^–1 Key words: Sink-source relationship, cotyledons, photosynthesis     

Effects of Ammonium and Potassium Ions on Some Physiological and Biochemical Processes of Excised Cucumber Cotyledons

Incubation of excised cucumber cotyledons (Cucumis sativus L.) with NH₄Cl solutions exceeding 0.001 M inhibited their greening, fresh weight increases, and incorporation of ¹⁴C-leucine into insoluble N compounds. The absorption of ¹⁴C-leucine during incubation and retention of moisture by the excised cotyledons after incubation were greatly diminished by the NH₄Cl treatments. Treatment with KCl solutions of the same concentrations as the NH₄Cl stimulated the greening, fresh weight increases, and the absorption and incorporation of ¹⁴C-leucine. Desiccation of cotyledons stored at 5°C for 10 days was inhibited by dilute KCl solutions. The toxicity of NH₄Cl was alleviated by KCl solutions at low concentrations.     

Characteristics of Root-to-Shoot Transport of Cytokinin 6-Benzylaminopurine in Intact Seedlings of Citrus aurantium

Transport of the cytokinin 6-benzylaminopurine-8-¹⁴C in the root and shoot of intact Citrus aurantium L. seedlings was studied by “replacing” the 0.5 cm root tip with the uptake solution. The cytokinin was transported basipetally in the root and was distributed in an acropetal direction in the stem and into the leaves. Kinetic analysis of the transport for periods of up to 96 h revealed a characteristic advancing front of the label along the axis of the seedling. The estimated velocity of transport of 6-benzylamino-purine-8-¹⁴C in various regions of the intact root was 2.6 to 5.1 mm/h. The transport of 6-benzylaminopurine was predominantly in the transpiration stream, in stelar tissues of the root. Conditions of high transpiration favored enhanced transport to the shoot and an overall greater accumulation of the label. The total accumulation of 6-benzylaminopurine in roots of intact seedlings after 48 h of transport was 354% of that in roots of shoot-less seedlings. Root girdling and treatment of the root with KCN did not reduce the basipetal transport of the label in the root and into the shoot. Radiochromalogram scanning of root extracts and analysis of the ethanol insoluble-NaOH soluble fraction revealed considerable metabolic changes in the translocated cytokinin. Only 51% of the radioactivity remained in the original 6-benzylaminopurine peak after 24 h of incubation. Two other, unidentified, metabolites were detected. It is suggested that all the factors that affect the ascent of sap are involved in the long-distance transport of cytokinins, and that the rate and mode of transport of cytokinins from the root system to the shoot may be a major factor in the expression of their physiological activity.     

两种生态型芦苇叶绿体的光合电子传递和抗氧保护体系

分布于甘肃省河西走廊的两种不同生态型芦苇-水生芦苇(水芦,生长在深约1m的水滩里)和沙丘芦苇(沙芦,生长在高约5m的沙丘上)在其离体叶绿体的光化学活性上表现为前者高于后者。其中,沙芦全链电子传递速率及光系统Ⅱ(PSⅡ)电子传递速率明显低于水芦,而光系统Ⅰ(PSⅠ)电子传递速率却与水芦接近。沙芦叶片和叶绿体中抗氧化酶活性及叶片抗坏血酸含量均高于水芦。综合结果表明,喜水植物芦苇登陆后,在自然选择压力下,为适应长期的自然干旱胁迫环境,其抗氧保护系统在保护PSⅠ免受水分胁迫诱导的氧化伤害中可能发挥着重要作用。     

Mathematical Simulation of Electron Transport in the Primary Photosynthetic Processes

Biochemistry (Moscow) - Summarized results of investigation of regulation of electron transport and associated processes in the photosynthetic membrane using methods of mathematical and computer...     

Hydrogen Peroxide Inhibits Photosynthetic Electron Transport in Cells of Cyanobacteria

The effect of H₂O₂ on photosynthetic O₂ evolution and photosynthetic electron transfer in cells of cyanobacteria Anabaena variabilis and Anacystis nidulans was studied. The following experiments were performed: 1) directly testing the effect of exogenous H₂O₂; 2) testing the effect of intracellular H₂O₂ generated with the use of methyl viologen (MV); 3) testing the effect of inhibiting intracellular H₂O₂ decomposition by salicylic acid (SA) and 3-amino-1,2,4-triazole (AT). H₂O₂ inhibited photosynthetic O₂ evolution and light-induced reduction of p-benzoquinone (BQ) + ferricyanide (FeCy) in the Hill reaction. The I₅₀ value for H₂O₂ was 0.75 mM. Photosynthetic electron transfer in the cells treated with H₂O₂ was not maintained by H₂O₂, NH₂OH, 1,5-diphenylcarbazide, tetraphenylboron, or butylated hydroxytoluene added as artificial electron donors for Photosystem (PS) II. The H₂O CO₂, H₂O MV (involving PSII and PSI) and H₂O BQ + FeCy (chiefly dependent on PSII) electron transfer reactions were inhibited upon incubation of the cells with MV, SA, or AT. The N,N,N",N"-tetramethyl-p-phenylenediamine MV (chiefly dependent on PSI) electron transfer was inhibited by SA and AT but was resistant to MV. The results show that H₂O₂ inhibits photosynthetic electron transfer. It is unlikely that H₂O₂ could be a physiological electron donor in oxygenic photosynthesis.     

Role of Nitrate in Photosynthetic Electron Transport of Chlorella Vulgaris

Addition of nitrate to a suspension of NO₃ ^--depleted Chlorella vulgaris cells raised the O₂-evolving capacity of the organism by 60%. The rate of O₂-evolution under flash irradiation of the depleted cells was drastically reduced, which could be restored by addition of NO₃ ^-. The 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB)-insensitive O₂-evolution, i.e., photosystem (PS) 2 activity of NO₃--depleted cells, showed a 75% stimulation by addition of NO₃ ^-. PS1-mediated electron transport was also stimulated (50%) by addition of NO₃ ^-. Fluorescence yields of the NO₃ ^--depleted cells were significantly reduced. A normal fluorescence response was restored by the addition of NO₃ ^-. The fluorescence yield of the NO₃ ^--depleted and DCMU-treated-cells increased significantly after addition of NO₃ ^- ions, indicating a further reduction of the primary acceptor of PS2 (Q). In addition, the low temperature fluorescence emission spectra showed that energy transfer to PS2 and PS1 was much higher when nitrate was present. Hence nitrate accelerates the light-induced charge transfer from the intact O₂-evolving system to the primary electron acceptor of PS2 and stimulates the PS1-mediated electron transport. This revised version was published online in June 2006 with corrections to the Cover Date.     

Specific Binding of Protoporphyrin IX to a Membrane-Bound 63 Kilodalton Polypeptide in Cucumber Cotyledons Treated with Diphenyl Ether-Type Herbicides

Porphyrin accumulation in excised cucumber cotyledons (Cucumis sativus L.) treated with a N-phenylimide S-23142 (N-[4-chloro-2-fluoro-5-propargyloxyphenyl]-3,4,5,6- tetrahydrophthalimide) and a diphenylether acifluorfen-ethyl (ethyl-5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitro benzoic acid) was studied. Most of the accumulated porphyrins were found in the membrane fractions of 6,000g and 30,000g pellets, forming a complex with a membrane polypeptide. The complex was solubilized with 1% n-dodecyl β-d-maltoside and its molecular mass was estimated to be 63,000 and 66,000 daltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel permeation high performance liquid chromatography (HPLC), respectively. The polypeptide also existed in untreated cotyledons but had little protoporphyrin IX. The complex was also formed in vitro by mixing the 30,000g pellets from untreated cotyledons and authentic protoporphyrin IX. However, protoporphyrin IX formed the complex specifically with the 63,000 dalton polypeptide and not with the other proteins both in vivo and in vitro. At least four fluorescent porphyrins, including protoporphyrin IX, were found in the acetone extract of the cotyledons by HPLC using a reversed phase column. Protoporphyrin IX was one of the two porphyrins that formed the complex. These results suggest that S-23142 and acifluorfenethyl enhance the accumulation of protoporphyrin IX, which forms the complex with the membrane protein.     

Photosynthetic and Dark Fixation of 14CO2 in Detached Soybean Cotyledons

Detached soybean cotyledons fixed CO₂ both in the light and dark. Carbon dioxide fixed by the light and dark reactions replaced only a small portion of CO₂ lost by respiration up to the 10th day. In the dark most of the ¹⁴C label was found in the acidic fraction, while in the light 65 per cent of the activity was found in the neutral and basic fractions.     

Responses of Photosynthetic Electron Transport to Drought and Re-watering in Two Maize Genotypes

Russian Journal of Plant Physiology - Effects of drought stress on photosynthesis have been well-documented. However, photosynthetic electron transport process in response to combined drought...     

6-BA和氨基酸对黄瓜子叶离体培养成花的影响

在基本培养基的筛选中,1/2MS培养基中附加0.10 mg·L-16-BA能显著提高离体黄瓜子叶的开花率,White培养基中附加2.00mg·L-1的KT下开花率也有明显提高,但植株矮小、瘦弱,整株变黄,甚至透明化,长势极差.浓度在一定范围的6-BA(0.01～0.50mg·L-1)对黄瓜子叶开花率影响不大,但在高浓度(0.50 mg·L-1)下,植株生长势差,开花迟,低浓度(0.01 mg·L-1)下,生长势较好.相同浓度的L-丙氨酸和L-酪氨酸均明显促进黄瓜子叶开花,且开花早;而甘氨酸对黄瓜子叶开花则有一定的抑制,开花率低.     

Control of Rooting and the Pattern of Senescence in Detached White Mustard Cotyledons

Senescence of detached mustard cotyledons was greatest in full light or at low light intensity but was reduced at intermediate light intensities. The location of yellowing in the blade depended on the conditions in which it was maintained after excision. Cotyledons cultured at full light intensity in the absence of exogenous nutrients formed roots at the base and yellowed around the margins. Reduction in light intensity and increasing nutrient concentrations resulted in an increasing proportion of the cotyledons yellowing from the base upwards, and fewer developed roots. At low light intensity with high nutrient levels rooting was suppressed and basal senescence predominated. The location of yellowing in the cotyledon may be associated with mobilisation gradients induced by the differential effects of light and mineral nutrient level on root initiation and blade growth.     

Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi VI. Electron Transport in Mutant Strains Lacking Either Cytochrome 553 or Plastocyanin

A mutant strain of Chlamydomonas reinhardi, ac-206, lacks cytochrome 553, at least in an active and detectable form. Chloroplast fragments of this mutant strain are inactive in the photoreduction of NADP when the source of electrons is water, but they are active when the electron source is 2,6-dichlorophenolindophenol and ascorbate. The addition of either cytochrome 553 or plastocyanin, obtained from the wild-type strain, has no effect upon the photosynthetic activities of the mutant strain. Cells of the mutant strain lack both the soluble and insoluble forms of cytochrome 553, but they possess the mitochondrial type cytochrome c. Thus, the loss of cytochrome 553 appears to be specific.