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1.
Salt adaptation of the cyanobacterium synechococcus 6311 growing in a continuous culture (turbidostat) 总被引:5,自引:1,他引:4
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Physiological aspects of salt-adaptation in the cyanobacterium Synechococcus 6311 growing in a continuous culture (turbidostat) were studied. The process of salt-adaptation was completed within 3 days, as expressed by the specific growth rate of cells grown in the presence of 0.2 and 0.4 molar NaCl. An increase in photosynthetic activity during the adaptation period leads to the accumulation of soluble sugars, essential for osmoregulation in the salt-grown cells. Cells grown in the presence of 0.4 molar NaCl showed an initial inhibition in the rate of protein synthesis which was enhanced after the 1st day of salt stress. After adaptation, salt-grown cells showed an increase in phycobiliprotein synthesis and a higher phycobiliprotein to protein ratio. 相似文献
2.
Na+ and Cl− are the principal solutes utilized for osmotic adjustment in cells of Nicotiana tabacum L. var Wisconsin 38 (tobacco) adapted to NaCl, accumulating to levels of 472 and 386 millimolar, respectively, in cells adapted to 428 millimolar NaCl. X-ray microanalysis of unetched frozen-hydrated cells adapted to salt indicated that Na+ and Cl− were compartmentalized in the vacuole, at concentrations of 780 and 624 millimolar, respectively, while cytoplasmic concentrations of the ions were maintained at 96 millimolar. The morphometric differences which existed between unadapted and salt adapted cells, (cytoplasmic volume of 22 and 45% of the cell, respectively), facilitated containment of the excited volume of the x-ray signal in the cytoplasm of the adapted cells. Confirmation of ion compartmentation in salt adapted cells was obtained based on kinetic analyses of 22Na+ and 36Cl− efflux from cells in steady state. These data provide evidence that ion compartmentation is a component of salt adaptation of glycophyte cells. 相似文献
3.
Wei Liu Xiaoting Yuan Yanyan Zhang Yanan Xuan Yongqing Yan 《Acta Physiologiae Plantarum》2014,36(8):2183-2193
Nitraria tangutorum Bobr. is a typical halophyte with superior tolerance to salinity. However, little is known about its physiological adaptation mechanisms to the salt environment. In the present study, N. tangutorum seedlings were treated with different concentrations of NaCl (100, 200, 300 and 400 mmol L?1) combined with five levels of Ca2+ (0, 5, 10, 15 and 20 mmol L?1) to investigate the effects of salt stress and exogenous Ca2+ on Na+ compartmentalization and ion pump activities of tonoplast and plasma membrane (PM) in leaves. Na+ and Ca2+ treatments increased the fresh weight and dry weight of N. tangutorum seedlings. The absorption of Na+ in roots, stems and leaves was substantially increased with the increases of NaCl concentration, and Na+ was mainly accumulated in leaves. Exogenous Ca2+ reduced Na+ accumulation in roots but promoted Na+ accumulation in leaves. The absorption and transportation of Ca2+ in N. tangutorum seedlings were inhibited under NaCl treatments. Exogenous Ca2+ promoted Ca2+ accumulation in the plant. Na+ contents in apoplast and symplast of leaves were also significantly increased, and symplast was the main part of Na+ intracellular compartmentalization. The tonoplast H+-ATPase and H+-PPase activities were significantly promoted under salt stress (NaCl concentrations ≤300 mmol L?1). PM H+-ATPase activities gradually increased under salt stress (NaCl concentrations ≤200 mmol L?1) followed by decreases with NaCl concentration increasing. The tonoplast H+-ATPase, H+-PPase and PM H+-ATPase activities increased first with the increasing exogenous Ca2+ concentration, reached the maximums at 15 mmol L?1 Ca2+, and then decreased. The tonoplast and PM Ca2+-ATPase activities showed increasing trends with the increases of NaCl and Ca2+ concentration. These results suggested that certain concentrations of exogenous Ca2+ effectively enhanced ion pump activities of tonoplast and PM as well as promoted the intracellular Na+ compartmentalization to improve the salt tolerance of N. tangutorum. 相似文献
4.
《生物化学与生物物理学报:生物膜》1985,814(2):347-355
The kinetics of uptake and loss of Na+ have been studied using radioisotopic tracer techniques in cells of the cyanobacterium Synechocystis PCC6714 exposed to hyperosmotic stress. Cells transferred from a fresh-water-based medium to NaCl at 300–1000 mmol·dm−3 showed net Na+ uptake during the first 2 min following transfer, with the intracellular Na+ level at 2 min increasing as a direct function of the external NaCl concentration. Further incubation of cells in low-level hypersaline media (350–500 mmol · dm−3 NaCl) led to a marked reduction in cell Na+ within 20 min, indicating an efficient active Na+ extrusion system. In contrast, cells maintained in more extreme hypersaline media showed little (750 mmol · dm−3 NaCl) or no (1000 mmol · dm−3 NaCl) net Na+ extrusion following upshock. Cells grown in a saline medium (with NaCl at 500 mmol · dm−3 showed a greatly reduced net Na+ uptake after 2 min in media containing higher levels of NaCl. However, net Na+ uptake was also observed when these cells were downshocked to media containing 50–200 mmol · dm−3 NaCl. This is the first demonstration of downshock-induced Na+ accumulation in a microbial cell. Time-courses for Na+ extrusion in cells downshocked from 500 mmol · dm−3 to 100 mmol · dm−3 NaCl were similar to those for cells upshocked from freshwater to 500 mmol · dm−3 NaCl, requiring approximately 30 min to reach their lowest values. Net Na+ extrusion in upshocked cells was found to be markedly sensitive to the external K+ concentration, with limited net Na+ extrusion in the absence of external K+ and maximal reductions in cell Na+ in media containing K+ at 1–10 mmol · dm−3. Temperature was also shown to affect uptake and loss of cell Na+ during upshock: cells maintained at 5°C showed no capacity for net Na+ extrusion, while higher temperatures (up to 35°C) led to a progressive reduction in the amount of cell Na+ at 2 min following upshock and also in the rate of net Na+ extrusion after this time. 相似文献
5.
《Journal of Fermentation and Bioengineering》1992,73(1):11-15
Two of five Zygosaccharomyces rouxii mutants defective in salt tolerance, 152S (sat1) and 1717S (SAT3), were inviable in a nutrient medium (YPD) containing more than 1% NaCl. These two mutant cells contained significantly higher amounts of Na+ (298 μmol and 285 μmol per g cells of 152S and 1717S, respectively) but lower amounts of K+ (242 μmol and 176 μmol per g cells of 152S and 1717S, respectively) than three other mutants, 41S (sat2-1 [98 μmol Na+ and 326 μmol K+/g cells]), 197S (sat2-2 [103μmol Na+ and 336 μmol K+/g cells]), 1611S (SAT4 [139 μmol Na+ and 294 μmol K+/g cells]), as well as a wild-type strain, AN39 (61 μmol Na+ and 349 μmol K+/g cells), when cultured in YPD medium containing 0.8% NaCl. A KCl supplement, optimally 0.6 M, added to the medium somewhat restored the NaCl-hypersensitivity of 152S and 1717S with a concomitant decrease of intracellular Na+. This finding suggests that the NaCl-hypersensitive mutations are due to a defect in the Na+-regulating mechanism. The other three mutants showed weak responses to KCl in high NaCl-YPD. These five salt sensitive mutants and the wild-type strain retained the same levels of intracellular glycerol and arabitol when transferred into NaCl (5%)-YPD from YDP medium. This suggests that polyol accumulation is not the only mechanism of salt tolerance in Z. rouxii. 相似文献
6.
Exposure to high concentrations of environmental NaCl exerts two stress effects on living cells, increasing the osmotic pressure and the concentration of inorganic ions. Salt stress dramatically suppresses the photosynthetic activity in cells of phototrophic organisms, such as cyanobacteria. During salt adaptation, cyanobacterial cells accumulate osmoprotectors, export excessive Na+ with the help of Na+/H+ antiporters, and actively absorb K+ with the help of K+-transporting systems. These physiological processes are accompanied by induction or suppression of several genes involved in salt adaptation. The review considers the main mechanisms responsible for the resistance of cyanobacterial cells to salt and hyperosmotic stresses. Special emphasis is placed on recent achievements in studying the genetic control of salt resistance and regulation of gene expression during adaptation of cyanobacteria to salt and hyperosmotic stresses. 相似文献
7.
Abscisic Acid Stimulated Osmotic Adjustment and Its Involvement in Adaptation of Tobacco Cells to NaCl 总被引:15,自引:3,他引:12
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Osmotic adjustment of cultured tobacco (Nicotiana tabacum L. var Wisconsin 38) cells was stimulated by 10 micromolar (±) abscisic acid (ABA) during adaptation to water deficit imposed by various solutes including NaCl, KCl, K2SO4, Na2SO4, sucrose, mannitol, or glucose. The maximum difference in cell osmotic potential (Ψπ) caused by ABA treatment during adaptation to 171 millimolar NaCl was about 6 to 7 bar. The cell Ψπ differences elicited by ABA were not due to growth inhibition since ABA stimulated growth of cells in the presence of 171 millimolar NaCl. ABA caused a cell Ψπ difference of about 1 to 2 bar in medium without added NaCl. Intracellular concentrations of Na+, K+, Cl−, free amino acids, or organic acids could not account for the Ψπ differences induced by ABA in NaCl treated cells. However, since growth of NaCl treated cells is more rapid in the presence of ABA than in its absence, greater accumulation of Na+, K+, and Cl− was necessary for ion pool maintenance. Higher intracellular sucrose and reducing sugar concentrations could account for the majority of the greater osmotic adjustment of ABA treated cells. More rapid accumulation of proline associated with ABA treatment was highly correlated with the effects of ABA on cell Ψπ. These and other data indicate that the role of ABA in accelerating salt adaptation is not mediated by simply stimulating osmotic adjustment. 相似文献
8.
Potato (Solanum tuberosum) is a major crop world-wide and the productivity of currently used cultivars is strongly reduced at high soil salt levels. We compared the response of six potato cultivars to increased root NaCl concentrations. Cuttings were grown hydroponically and treated with 0 mM, 60 mM and 180 mM NaCl for one week. Growth reduction on salt was strongest for the cultivars Mozart and Mona Lisa with a severe senescence response at 180 mM NaCl and Mozart barely survived the treatment. The cultivars Desiree and Russett Burbank were more tolerant showing no senescence after salt treatment. A clear difference in Na+ homeostasis was observed between sensitive and tolerant cultivars. The salt sensitive cultivar Mozart combined low Na+ levels in root and stem with the highest leaf Na+ concentration of all cultivars, resulting in a high Na+ shoot distribution index (SDI) for Mozart as compared to Desiree. Overall, a positive correlation between salt tolerance and stem Na+ accumulation was found and the SDI for Na+ points to a role of stem Na+ accumulation in tolerance. In stem tissue, Mozart accumulated more H2O2 and less proline compared to the tolerant cultivars. Analysis of the expression of proline biosynthesis genes in Mozart and Desiree showed a clear reduction in proline dehydrogenase (PDH) expression in both cultivars and an increase in pyrroline-5-carboxylate synthetase 1 (P5CS1) gene expression in Desiree, but not in Mozart. Taken together, current day commercial cultivars show promising differences in salt tolerance and the results suggest that mechanisms of tolerance reside in the capacity of Na+ accumulation in stem tissue, resulting in reduced Na+ transport to the leaves. 相似文献
9.
Sawan Kumar Sathyanarayana N. Gummadi 《Extremophiles : life under extreme conditions》2009,13(5):793-805
Debaryomyces nepalensis NCYC 3413, a food spoiling yeast isolated from rotten apple, has been previously demonstrated as halotolerant yeast. In the
present study, we assessed its growth, change in cell size, and measured the intracellular polyol and cations (Na+ or K+) accumulated during growth in the absence and presence of different concentrations of salts (NaCl and KCl). Cells could tolerate
2 M NaCl and KCl in defined medium. Scanning electron microscopic results showed linear decrease in mean cell diameter with
increase in medium salinity. Cells accumulated high amounts of K+ during growth at high concentrations of KCl. However, it accumulated low amounts of Na+ and high amounts of K+ when grown in the presence of NaCl. Cells grown in the absence of salt showed rapid influx of Na+/K+ on incubation with high salt. On incubation with 2 M KCl, cells grown at 2 M NaCl showed an immediate efflux of Na+ and rapid uptake of K+ and vice versa. To withstand the salt stress, osmotic adjustment of intracellular cation was accompanied by intracellular
accumulation of polyol (glycerol, arabitol, and sorbitol). Based on our result, we hypothesize that there exists a balanced
efflux and synthesis of osmolytes when D. nepalensis was exposed to hypoosmotic and hyperosmotic stress conditions, respectively. Our findings suggest that D. nepalensis is an Na+ excluder yeast and it has an efficient transport system for sodium extrusion. 相似文献
10.
NaCl胁迫下沙枣幼苗生长和阳离子吸收、运输与分配特性 总被引:18,自引:0,他引:18
沙枣(Elaeagnus angustifolia L.)耐盐性强,是我国北方生态脆弱地区造林绿化的一个先锋树种。为探讨沙枣的盐适应机制,研究了不同浓度NaCl(0、100和200 mmol/L)胁迫30d对其水培幼苗生物量累积以及不同组织(根、茎、叶)K+、Na+、Ca2+和Mg2+吸收、运输与分配的影响。结果表明:盐胁迫不同程度地促进了沙枣苗根系生长;100 mmol/L NaCl胁迫对幼苗生物量累积无明显影响,而200 mmol/L则显著抑制了生物量累积;盐胁迫幼苗根、茎、叶中Na+含量以及K+-Na+选择性运输系数(S K,Na)和Ca2+-Na+选择性运输系数(S Ca,Na)显著或大幅度增加,而K+、Ca2+、Mg2+含量以及K+/Na+、Ca2+/Na+和Mg2+/Na+比值则显著或大幅度下降;200 mmol/L NaCl胁迫沙枣根Na+含量和根Na+净累积量分别为22.15 mg/g干重和1.87 mg/株(是对照的16.20倍和20.06倍),根成为Na+净累积量增加幅度最大的组织和Na+含量最高的组织;200 mmol/L NaCl胁迫沙枣茎、叶中的Na+含量以及冠组织Na+净累积量分别高达5.15、7.71 mg/g干重和3.29 mg/株(是对照的7.22倍、9.58倍和5.45倍),但幼苗仍能正常生长。综合分析认为,沙枣的盐适应机制是根系拒盐和冠组织耐盐,主要通过根系的补偿生长效应、根系对Na+的聚积与限制作用以及冠组织对Na+的忍耐来实现的,同时也与根、茎和叶对K+、Ca2+选择性运输能力显著增强有关。 相似文献
11.
Excised barley (Hordeum vulgare var Larker) leaves were treated with salt solutions or wilted. After the treatment period, the leaves were allowed to recover in a 50 millimolar sucrose and 1 millimolar glutamate solution, and proline, Na+, and K+ were measured at intervals. Na+ and K+ concentrations stayed at a constant high level after the salt treatments, and proline increased to a steady state concentration in response. The relationship between the maximum rate of proline accumulation and the Na+ concentration reached in each experiment was linear. The final steady state proline concentration reached was also directly proportional to the Na+ concentration. For a given Na+ concentration in the leaves, the steady state proline level was greater when 410 millimolar NaCl was added to the leaves than when 205 millimolar NaCl was added. These results are consistent with proline acting as a compatible cytoplasmic solute, balancing an accumulation of salts outside of the cytoplasm.
In contrast to the proline levels in salt-shocked leaves, the concentrations in wilted leaves decreased to near control levels within 24 hours of relief of stress.
相似文献12.
Tatiana Leonova Vera Ovchinnykova Erik Souer Albert de Boer Petr Kharchenko Alexey Babakov 《Plant signaling & behavior》2009,4(11):1059-1062
Shoots of Thellungiella derived by micropropagation were used to estimate the plants'' salt tolerance and ability to regulate Na+ uptake. Two species with differing salt tolerances were studied: Thellungiella salsuginea (halophilla), which is less tolerant, and Thellungiella botschantzevii, which is more tolerant. Although the shoots of neither ecotype survived at 700 mM NaCl or 200 mM Na2SO4, micropropagated shoots of T. botschantzevii were more tolerant to Na2SO4 (10–100 mM) and NaCl (100–300 mM). In the absence of roots, Na2SO4 salinity reduced shoot growth more dramatically than NaCl salinity. Plantlets of both species were able to adapt to salt stress even when they did not form roots. First, there was no significant correlation between Na+ accumulation in shoots and Na+ concentration in the growth media. Second, K+ concentrations in the shoots exposed to different salt concentrations were maintained at equivalent levels to control plants grown in medium without NaCl or Na2SO4. These results suggest that isolated shoots of Thellungiella possess their own mechanisms for enabling salt tolerance, which contribute to salt tolerance in intact plants.Key words: Thellungiella salsuginea, Thellungiella botschantzevii, salt tolerance, isolated shoots, growth, rhizogenesis, ion accumulation 相似文献
13.
Determination of Ion Content and Ion Fluxes in the Halotolerant Alga Dunaliella salina 总被引:11,自引:6,他引:5
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A method to determine intracellular cation contents in Dunaliella by separation on cation-exchange minicolumns is described. The separation efficiency of cells from extracellular cations is over 99.9%; the procedure causes no apparent perturbation to the cells and can be applied to measure both fluxes and internal content of any desired cation. Using this technique it is demonstrated that the intracellular averaged Na+, K+, and Ca2+ concentrations in Dunaliella salina cultured at 1 to 4 molar NaCl, 5 millimolar K+, and 0.3 millimolar Ca2+ are 20 to 100 millimolar, 150 to 250 millimolar, and 1 to 3 millimolar, respectively. The intracellular K+ concentration is maintained constant over a wide range of media K+ concentrations (0.5-10 millimolar), leading to a ratio of K+ in the cells to K+ in the medium of 10 to 1,000. Severe limitation of external K+, induces loss of K+ and increase in Na+ inside the cells. The results suggest that Dunaliella cells possess efficient mechanisms to eliminate Na+ and accumulate K+ and that intracellular Na+ and K+ concentrations are carefully regulated. The contribution of the intracellular Na+ and K+ salts to the total osmotic pressure of cells grown at 1 to 4 molar NaCl, is 5 to 20%. 相似文献
14.
Induction of salt tolerance in Azolla microphylla Kaulf through modulation of antioxidant enzymes and ion transport 总被引:1,自引:0,他引:1
Azolla microphylla plants exposed directly to NaCl (13 dsm-1) did not survive the salinity treatment beyond a period of one day, whereas plants exposed directly to 4 and 9 dsm-1 NaCl were able to grow and produce biomass. However, plants pre-exposed to NaCl (2 dsm-1) for 7 days on subsequent exposure to 13 dsm-1 NaCl were able to grow and produce biomass although at a slow rate and are hereinafter designated as pre-exposed plants. The pre-exposed and directly exposed plants distinctly differed in their response to salt in terms of lipid peroxidation, proline accumulation, activity of antioxidant enzymes, such as SOD, APX, and CAT, and Na+/K+ ratio. Efficient modulation of antioxidant enzymes coupled with regulation of ion transport play an important role in the induction of salt tolerance. Results show that it is possible to induce salt adaptation in A. microphylla by pre-exposing them to low concentrations of NaCl. 相似文献
15.
It has been shown that the intracellular concentrations of Na+, K+, and Cl? ions in Desulfonatronum thiodismutans depend on the extracellular concentration of Na+ ions. An increase in the extracellular concentration of Na+ results in the accumulation of K+ ions in cells, which points to the possibility that these ions perform an osmoprotective function. When the concentration of the NaCl added to the medium was increased to 4%, the concentration gradient of Cl? ions changed insignificantly. It was found that D. thiodismutans contains two forms of hydrogenase—periplasmic and cytoplasmic. Both enzymes are capable of functioning in solutions with high ionic force; however they exhibit different sensitivities to Na+, K+, and Li+ salts and pH. The enzymes were found to be resistant to high concentrations of Na+ and K+ chlorides and Na+ bicarbonate. The cytoplasmic hydrogenase differed significantly from the periplasmic one in having much higher salt tolerance and lower pH optimum. The activity of these enzymes depended on the nature of both the cationic and anionic components of the salts. For instance, the inhibitory effect of NaCl was less pronounced than that of LiCl, whereas Na+ and Li+ sulfates inhibited the activity of both hydrogenase types to an equal degree. The highest activity of these enzymes was observed at low Na+ concentrations, close to those typical of cells growing at optimal salt concentrations. 相似文献
16.
Joaquim A. G. Silveira José M. Júnior Evandro N. Silva Sérgio L. Ferreira-Silva Rafael M. Arag?o Ricardo A. Viégas 《Acta Physiologiae Plantarum》2012,34(5):1629-1637
This study establishes relationships between salt resistance and solute accumulation in roots and leaves of two contrasting cashew species. The sensitive (Anacardium microcarpum) and resistant (A. occidentale) species showed maximum root LD50 values (the external NaCl concentration required for a 50% reduction in dry weight) of 63 and 128?mM NaCl, whereas the shoot LD50 values were 90 and 132?mM, respectively. The salt sensitivity was directly associated with Na+ accumulation and especially with the Cl? content in leaves and to a minor extent in roots. The accumulation of saline ions was associated with higher net uptake rates by roots and transport rates from root to shoot in the sensitive cashew species. The K+/Na+ ratios were not associated with salt resistance either in roots or leaves. Proline and free amino acid concentrations were strongly increased by salinity, especially in the leaves of the resistant species. The soluble sugar concentrations were not influenced by NaCl treatments in leaves of both species. In contrast, the root soluble sugar content was significantly decreased by salinity in the sensitive species only. In conclusion, the higher salt sensitivity of A. microcarpum is associated to an inefficient salt exclusion system of the leaves, especially for Cl?. On the other hand, the resistant species displays higher concentrations of organic solutes especially a salt-induced accumulation of proline and free amino acids in leaves. 相似文献
17.
The effect of different growth conditions on the activity of the Na+/H+ antiporter in Dunaliella salina has been investigated. Adaptation of D. salina cells to ammonia at alkaline pH or to high NaCl concentrations is associated with a pronounced increase in the plasma membrane Na+/H+ exchange activity. The enhanced activity is manifested both in vivo, by stimulation of Na+ influx into intact cells in response to internal acidification, and in vitro, by a larger 22Na accumulation in plasma membrane vesicles in response to an induced pH gradient. Kinetic analysis shows that the stimulation does not result from a change of the Km for Na+ but from an increase in the Vmax. In contrast, adaptation of cells to a high LiCl concentration (0.8 m) depresses the activity of the Na+/H+ antiporter. Adaptation to ammonia is also associated with a large increase of three polypeptide bands in purified plasma membrane preparations, indicating that they may compose the antiporter polypeptides. These results suggest that adaptation to ammonia or to high salinity induces overproduction of the plasma membrane Na+/H+ antiporter in Dunaliella. 相似文献
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20.
Accumulation of potassium and sodium by barley roots in a k-na replacement series 总被引:1,自引:1,他引:0
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Hiatt AJ 《Plant physiology》1969,44(11):1528-1532
Excised roots of barley (Hordeum vulgare, var. Campana) were incubated for 24 hr in solutions containing constant total concentrations of KCl and NaCl but in which the mole fractions of K and Na were varied in replacement series. In solutions containing 1, 10, or 50 mm concentrations of K+ plus Na+, total cation accumulation was dependent upon the total salt concentration but was relatively independent of the mole fractions of K+ and Na+. These results imply that accumulation of K+ and Na+ was limited by a common factor. In solutions containing 0.01 mm K+ plus Na+ there was a strong preference for K+ over Na+ and the sum of K+ and Na+ accumulation increased with increasing K+ concentration. 相似文献