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1.
The flame bulb is formed by a terminal cell and a proximal canal cell. The weir consists of interdigitating ribs all of which form one circle, i.e. alternating ribs do not have distinctly 'internal' or 'external' positions. Cytoplasmic cords are absent and all ribs, i.e. those continuous with the proximal canal cell and those continuous with the terminal cell, form external leptotriches. At least some external leptotriches have interconnected branches extending along the flame bulb. Internal leptotriches are not branched and arise from the basal perikaryon of the terminal cell. In the cytoplasmic cylinder at the tip of the flame bulb, structures resembling incomplete septate junctions were seen. However, neither the cytoplasmic cylinder nor the small protonephridial capillaries contain complete septate junctions as found in all other Monogenea Polyopisthocotylea, Monogenea Monopisthocotylea, Trematoda Aspidogastrea and Trematoda Digenea examined to date. In the lack of a septate junction, Anoplodiscus resembles Udonella, Amphilinidea, Gyrocotylidea and Eucestoda. However, the presence in this species of rudimentary septate junctions in the small capillaries and of complete junctions in larger ones indicates that complete junctions have been secondarily lost. Anoplodiscus resembles the Monogenea and Trematoda in the presence of lamellae in the larger protonephridial ducts. For the first time in a monogenean, the ultrastructure of the excretory bladder is described. A nucleated convoluted duct opens through a narrow connecting duct into the bladder, which in turn opens through a narrow connecting duct into the excretory pore lined by tegument. Convoluted duct, connecting ducts and bladder are lined by a lamellated epitheliu.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

2.
The ultrastructure of flame bulbs and epithelium of excretory canals in Bothrioplana semperi (Turbellaria, Seriata) have been studied. The flame bulbs consist of two cells, the terminal cell and the proximal canal cell. The weir is formed by two rows of longitudinal ribs. The ribs of the internal row originate from the flame cell, external ribs are formed by the proximal canal cell. Each external rib has a remarkable bundle of microfilaments, originating in the cytoplasm of the first canal cell distally to the bases of external ribs. Membrane of internal ribs is marked by small electrondense granules, separate or fused to an electron-dense layer, continuous to dense “membrane,” connecting both external and internal ribs. Sparse internal leptotrichs originate from the bottom of the flame bulb cavity. External leptotrichs are lacking. Septate junction is present only in proximal canal cell at the level of tips of cilia. The apical surface of the canal cell bears rare short microvilli. The basal membrane of canal cells forms long invaginations that may reach nearly the apical membrane. The epithelium of excretory canals lacks the cilia. The ultrastructure of flame bulbs and epithelium of the excretory canals in B. semperi shares representatives of suborder Proseriata (Seriata). The contradiction exists in interpretation of the structure of flame bulbs in Proseriata. Ehlers and Sopott-Ehlers assumed that the external ribs are derivatives of the proximal canal cell and internal ones are outgrowths of the terminal cell, while Rohde has found conversely: the external ribs are outgrowths of the terminal cell, the internal ones are outgrowths of the proximal canal cell. However, the illustrations provided by Rohde do not enable to ascertain what cells the internal and external ribs derive from, while illustrations provided by Ehlers justify his interpretation. The order of weir formation in B. semperi confirms the viewpoint of Ehlers. The implication of ultrastructure of flame bulbs in Proseriata, especially of the order of flame bulb formation, in the Platyhelminthes phylogeny has been discussed.  相似文献   

3.
本研究应用透射电子显微镜研究了扩张莫尼茨绦虫原肾管的细胞学特征 ,莫尼茨绦虫原肾管的焰茎球为一个过滤器结构 ,类似于“挡河坝”样构造 ,此构造由端细胞和近管细胞外突形成的肋条 (或称杆 )相互交错排列而成。肋条之间由细胞外物质构成的“膜”结构连接 ,过滤作用通过该“膜”发生。焰细胞与近管细胞交界处有裂缝或孔与细胞外的结缔组织 (实质组织 )相通 ;原肾管的毛细排泄管细胞质索之间没有隔状联结 ;毛细排泄管及排泄管的管腔内有大量珠状微绒毛突起以增加表面积。从扩张莫尼茨绦虫及其它一些无脊椎动物原肾管的研究结果表明 ,原原肾管概念将焰细胞作为封闭的盲端已不再合适 ,需要进行修订 ,建议修订为 :原肾管是一种焰细胞系统 ,通常由焰细胞、管细胞和肾孔细胞组成 ,焰茎球作为过滤装置与周围的结缔组织 (实质组织 )有或没有裂缝 (孔 )相通  相似文献   

4.
The ultrastructure of the epidermis and the protonephridia of the free-living rhabdocoel Mesoscastrada führmanni is described. The epidermis consists of polarized cells, the nucleus located in the basal part of the cell and the mitochondria in the apical part. The surface is entirely covered by cilia anchored in the cytoplasm by horizontal and vertical striated rootlets. Cilia of the flame bulbs also have horizontal and vertical striated rootlets. The weir apparatus of the cyrtocyte is composed of a single row of ribs connected by a thin “membrane” of extracellular material. Bundles of microtubules, located in the ribs originate in the centrioles. Epidermal cells and flame bulbs of M. führmanni closely resemble those of the other Typhloplanoida examined so far.  相似文献   

5.
The flame bulb of Prorhynchus is formed by a single cell. Its nucleus is not located in the cytoplasm at the base of the flame. Cilia of the flame have cross-striated hollow ciliary rootlets converging towards their tips. The maximum number of cilia counted was 13. The weir consists of a single row of longitudinal ribs that contain longitudinal filaments and possess regularly arranged protrusions along their surface appearing as transverse bands in horizontal section. A 'membrane' of extracellular material extends between the ribs. and loose material fills the places between the ribs, with a denser layer midway between adjacent ribs. Distally, the ribs fuse to form a continuous tube without a junction. Small protonephridial capillaries lack junctions, larger ducts have lateral flames and patches of long microvilli. Large excretory ducts open into a ciliated and lamellated cavity which is connected by a narrow canal to the excretory pore. The terminal part of the canal close to the pore possesses many cilia and microvilli. Phylogenetic implications of the findings are discussed.  相似文献   

6.
The ultrastructure of the flame bulbs of the turbellarian Urastoma cyprinae from Mytilus galloprovincialis in the Mediterranean is described. The nucleus of the terminal cell is located some distance basal to the rootlets of the cilia forming the flame; the cytoplasm contains numerous tubules approximately 54–66 nm in diameter, and vesicles. Thick walled, densely packed rod-like structures coil around each other with a tendency towards longitudinal orientation close to the flame. The rod-like structures tightly surround the basal part of the flame and the distal cytoplasmic tube in the apical part of the flame. Some of them, including the inner predominantly longitudinally directed ones, are continuous with the cytoplasm of the terminal cell, others are continuous with the cytoplasm of the distal cytoplasmic tube. Internal leptotriches arise from the cytoplasm of the terminal cell and intrude between the basal parts of the cilia of the flame. The distal cytoplasmic tube possesses a septate junction. The flame bulb of Urastoma differs distinctly from those known from other Platyhelminthes; implications for the phylogeny of Platyhelminthes are discussed.  相似文献   

7.
K. Rohde  N. Watson 《Acta zoologica》1991,72(3):137-142
The terminal part of the protonephridia of Microstomum is formed by a branching proximal canal cell and (at least?) two terminal cells. Each weir consists of longitudinal (sometimes convoluted) ribs continuous with the cytoplasm of the terminal cell. Internal leptotriches arise from the terminal and proximal canal cells. Near the tip of the flame, the proximal canal cell tube is surrounded by the more external terminal cell and connected to it by a septate junction. Large cristate mitochondria are densely packed in the terminal and canal cells. The flame bulb of Microstomum differs markedly from that of other macrostomids (Macrostomum, Paramalostomum) examined. Phylogenetic implications are discussed.  相似文献   

8.
Summary The ultrastructure of the flame bulbs, protonephridial capillaries and duct of fully developed and regenerating Stenostomum sp. is described. Flame bulbs are formed by a single cell whose nucleus is located basally or laterally to the weir. The weir is formed by a single row of transverse ribs connected by a thin membrane, apparently of extracellular matrix. Internal leptotriches arise from the proximal cytoplasm and extend in a (usually) single row along the weir and into the lumen of the distal cytoplasmic tube. Many or all leptotriches do not fuse with the distal cytoplasm. Two cilia are each anchored in the proximal cytoplasm by a cross-striated vertical and lateral rootlet, the latter bent forward and extending for some distance into one of the two cytoplasmic cords along the weir. Each cord contains the lateral rootlet in its proximal part, as well as many microtubules. The distal cytoplasmic tube contains two (longitudinal) junctions, i.e. lines of contact between cell processes of the same, terminal cell. Occasionally, more than two junctions were seen, apparently due to branches of the terminal cell in contact with each other. Flame bulbs join capillaries lined by several canal cells type I, containing few or no microvilli but lateral flames. Such capillaries join a duct (or ducts?) lined by canal cells type II with many long microvilli. The large protonephridial duct is lined by numerous cells with lateral flames and many long microvilli. In regenerating tissue (10.5 hours after cutting) some flame bulbs were free, i.e. not connected to capillaries, and some capillaries openly communicated with the surrounding intercellular space. In the presence of a single row of ribs in the weir, of internal leptotriches, and of vertical and lateral ciliary rootlets, the flame bulb of Stenostomum sp. resembles that of other Plathelminthes much more closely than hitherto thought. The species differs from non-catenulid plathelminths mainly in the large number of glandular cells lining the large protonephridial ducts, in the transverse orientation of the ribs in the weir and in the presence of only two cilia in the flame.  相似文献   

9.
Many important relationships amongst kinetoplastids, including the position of trypanosomatids, remain uncertain, with limited taxon sampling of markers other than small subunit ribosomal RNA (SSUrRNA). We report gene sequences for cytosolic heat shock proteins 90 and/or 70 (HSP90, HSP70) from the potentially early-diverging kinetoplastids Ichthyobodo necator and Rhynchobodo sp., and from bodonid clades ‘2’ (Parabodonidae) and ‘3’ (Eubodonidae). Some of the new cytosolic HSP70 sequences represent a distinct paralog family (HSP70-B), which is related to yet another paralog known from trypanosomatids (HSP70-C). The (HSP70-B, HSP70-C) clade seemingly diverged before the separation between kinetoplastids and diplonemids. Protein phylogenies support the basal placement of Ichthyobodo within kinetoplastids. Unexpectedly, Rhynchobodo usually forms the next most basal group, separated from the clade ‘1’ bodonids with which it has been allied. Bootstrap support is often weak, but the possibility that Rhynchobodo represents a separate early-diverging lineage within core kinetoplastids deserves further testing. Trypanosomatids always fall remote from the root of kinetoplastids, forming a specific relationship with bodonid clades 2 (and 3), generally with strong bootstrap support. These protein trees with improved taxon sampling provide the best evidence to date for a ‘late’ emergence of trypanosomatids, contradicting recent SSUrRNA-based proposals for a relatively early divergence of this group.  相似文献   

10.
Combined use of the intraaxonal retrograde transport of the fluorescent marker ‘true blue’ with substance P (SP) immunocytochemistry has been used to trace the nodose ganglion projections of SP-containing neurons of the aortic depressor nerve. It has been found that (1) SP immunoreactive (SP-I) cell bodies are clearly demonstrable in clusters in the rostral part of the nodose ganglion without the aid of colchicine pretreatment; (2) ‘true blue’ is retrogradely transported to the nodose ganglion following its application to the central cut end of the aortic nerve; (3) ‘true blue’ fluorescence and SP fluorescent immunoreactivity can be visualized in the same tissue section and certain cell bodies in the nodose ganglia contain both SP-I and retrogradely transported ‘true blue’. These results indicate that the aortic nerve which projects from the aortic arch baro- and/or chemoreceptors to brainstem vasomotor centers contains SP-I afferent fibers which emanate from the nodose ganglion.  相似文献   

11.
An electron microscopic study of the basal bodies of the Vibrio albinolyticus flagellum revealed a four-disc structure. The diameters of the two discs localized closer to the cytoplasmic membrane proved to be about 2-fold shorter than those of the two others. In this respect the basal body of V. alginolyticus resembles very much that of V. cholerae described by Ferris and co-workers. The sequence of the V. alginolyticus ribosomal 5S-RNA showed that it is similar to those of V. cholerae, V. harveyi and some other vibriones. On the basis of the 5S-RNA sequences, a dendrogram of prokaryotes is presented. It confirmed the suggestion that V. alginolyticus is a typical representative of Vibrionaceae rather than a ‘monster’ greatly differing from other vibriones. Possible evolutionary relation of various bacterial species possessing the primary Na+ pumps is discussed.  相似文献   

12.
The ultrastructure of the vas deferens, testes, spermatogenesis and spermatozoa of Gyrocotyle urna and G. parvispinosa is described. The vas deferens is ciliated and syncytial. Within the testes primary spermatocytes arise from the primary spermatogonia by incomplete mitotic divisions; the primary spermatocytes undergo two meiotic divisions leading to spermatids. In early spermatids microtubules are formed at the cell periphery. Later the spermatozoal cytoplasm (the ‘middle-piece’) grows out and the two spermatozoal flagella with their typical 9 + ‘1’ axonemes are formed. During ciliogenesis the flagella are at an angle of about 60° to the axis of the middle-piece. The flagella are inserted into basal bodies terminating in striated rootlets. Subsequently, the nucleus and isolated mitochondria migrate into the central axis. The angle between the flagella and the axis decreases; the flagella are incorporated to form the spermatozoon. In mature spermatozoa no basal body or rootlet elements were found. The phylogeny of parasitic Platyhelminthes is discussed with respect to the evolution of spermatozoa. The reduction of the acrosinoid granules which are found in spermatozoa of free-living Platyhelminthes and the incorporation of the spermatozoal flagella into the sperm body constitute autapomorphies of the Neodermata (the parasitic Platyhelminthes). Included in the Cestoda because of several common derived characters, Amphilinidea and Gyrocotylidea are the only cestodes with spermatozoa containing mitochondria. Their absence in Cestoidea—all taxa with a six-hooked larva and other characteristics—is an autapomorphy of this group.  相似文献   

13.
Poplar cuttings of a resistant clone, Populus ‘Grandis’, and susceptible clones, Populus nigra ‘Italica’ and Populus ‘Robusta’, were infected with the pathogenic fungus Dothichiza populea alone, or with the pathogen and one of five strains of epiphytes antagonistic towards it (in vitro), isolated from poplar bark. The extent of injury was examined for 28 days after infection by determining the length of necrotic patches and their area as expressed in per cent of the total area of a cutting or the area of necrotic injuries caused by the pathogen alone.

All the poplar cuttings of both the resistant and susceptible clones became diseased when infected with the pathogen alone. Surprisingly enough, however, the least affected clone was the susceptible P. ‘Robusta’, in which necrotic injuries covered 28% of the total area, as against 40% and 70% in the resistant P. ‘Grandis’ and the susceptible P. nigra ‘Italica’, respectively.

When the cuttings were infected simultaneously with Dothichiza populea and its antagonistic epiphytes, the diseased area in the resistant clone diminished by as much as two-thirds, and in the susceptible P. nigra ‘Italica’, by one-third in comparison with the area affected by the pathogen alone. In turn, in the susceptible P. ‘Robusta’ the introduction of three out of five epiphytes stimulated the growth of the pathogenic fungus producing on average a double increase in the necrotic area. The differences in the response of the pathogen to the presence of epiphytes recorded in the susceptible clones indicate a marked influence of the plant on the nature of interactions between its epiphytic microflora and the pathogen.  相似文献   


14.
K. Rohde 《Zoomorphology》1987,106(6):346-351
Summary The ultrastructure of the flame cells and protonephridial capillaries of the Rhabdocoela Craspedella sp. and Didymorchis sp., ectocommensals on the freshwater crayfish Cherax destructor in eastern Australia is described. The flame cells of both species have variable numbers of cilia without distinct rootlets and with decreasing numbers of axonemal tubules towards the ciliary tips. Bundles of microtubules extend from the cytoplasm adjacent to the ciliary rootlets through the ribs of the weir apparatus into the distal cytoplasmic tube, where the numbers of microtubules gradually decrease. The weir apparatus is formed by a single row of longitudinal ribs connected by a membrane. In Craspedella, but not in Didymorchis, the ribs have external branched leptotriches. Mitochondria are common in the wall of the flame cell of both species. The protonephridial capillary just above the end of the ciliary tuft narrows in both species and bends sharply in Craspedella. The lumen of the flame cell and the capillary is lined by a dark layer of cytoplasm; there is no enlargement of the surface area by microvilli or lamellae. Centrioles were seen in the capillary wall of Craspedella, and in Didymorchis the cytoplasm around the capillaries has a very loose and light appearance. The ultrastructure of the flame cells and capillaries of both species corresponds closely to that of Temnocephala sp.Abbreviations in the figures BB basal body - CE centriole - L leptotrich - M microtubules - ME membrane of weir apparatus - MI mitochondrion - PC protonephridial capillary - R rib (rod) of weir apparatus  相似文献   

15.
and 1988. Aspects of the life history of Cercopithifilaria johnstoni (Nematoda:Filarioidea). International Journal for Parasitology 18: 1087–1092. Cercopithifilaria johnstoni (Nematoda:Filarioidea) occurs in the subcutaneous connective tissues of a spectrum of native murid and marsupial hosts in Eastern Australia. Life cycle studies revealed that: (i) microfilaria occur in lymphatic capillaries and extravascular connective tissue of the dermis (= ‘skin-inhabiting’), (ii) ixodid ticks, particularly Ixodes trichosuri, are intermediate hosts in nature, (iii) development from microfilariae to infective third-stage larva occurs only while the tick is off the host, that is, during ecdysis from larva to nymph or from nymph to adult. Transmission of C. johnstoni in a wild population of bush rats (Rattus fuscipes) occurred in summer and winter, and was associated with peaks in the number of larval and/or nymphal stages of ticks on rats. C. johnstoni was transmitted experimentally to bandicoots (Isoodon macrourus, Perameles nasuta), bush rats and laboratory rats (R. norvegicus), indirectly by subcutaneous inoculation of third-stage larvae and directly by tick feeding. The prepatent period was approximately 3 months and the longest duration of microfilariae in the ‘ skin’ was more than 25 months. Dermal and ocular lesions were observed in R. fuscipes. The host-parasite relationship has the potential for development as an inexpensive and practical model for human onchocerciasis.  相似文献   

16.
1972. Double infection experiments with echinostomatids (Trematoda) in Lymnaea stagnalis by implantation of rediae and exposure to miracidia. International Journal for Parasitology, 2: 409–423. Echinostomatid species parasitizing Lymnaea stagnalis as first intermediate hosts in a South German Lake have been found present in natural double infections, but at frequencies lower than expected. Simultaneous double infection and superinfection experiments in Lymnaea stagnalis with Isthmiophora melis, Echinoparyphium aconiatum and Echinostoma revolutum were performed by redial implantation and by exposure to miracidia. All three combinations possible of these echinostomatids proved to be unstable, one species being eliminated by another ‘stronger’ one after an invariable suppression order. The degree of vigour of Isthmiophora melis in this suppression order is greater if mother rediae (macropharyngeate) are present, i.e. after miracidial invasion instead of daughter redial implantation. Snails parasitized by rediae of a ‘weak’ type could be superinfected by implantation of rediae of a ‘strong’ type, but not if the first (‘weak’) infection had reached the stage of shedding cercariae. Superinfection by implantation of Echinoparyphium aconiatum rediae (‘strong’ type) was not successful when the first infection consisted of sporocysts of plagiorchiids, or of Apatemon sp. (Strigeidae) that had reached the stage of shedding cercariae.  相似文献   

17.
Recent results are discussed which have led to a two-step model for UV mutagenesis in excision-deficient Escherichia coli. After exposure to UV, the replication fork is assumed to continue until immediately before certain photoproducts where it stops and leaves a gap which cannot be dealt with by recombination repair. In the first (misincorporation) step, bases (a proportion of which are ‘wrong’) are postulated to be inserted opposite the photoproduct under the direct influence of the recA gene product. These misincorporated bases can be revealed as mutations by delayed photoreversal in umuD, C and lexA (ind) bacteria. Their level is determined by the particular allele of recA that is present (recA441 > recA+ > recA430) and their rate of formation by the amount of recA protein in the cell and the degree of enrichment of the medium. No other protein needs to be synthesized for this step to occur. The second (bypass) step requires induced levels of the products of the umuD and C genes which are postulated to facilitate continued DNA synthesis on the priming end opposite the photoproduct. In principle, further errors could be made at this stage which might appear as ‘hitch-hiking’ rather than ‘targeted’ mutations.  相似文献   

18.
We have previously demonstrated that the C-terminal regions of the rat and human pancreatic polypeptide (PPP) precursors exhibit a high degree of divergence, whereas the N-terminal regions are highly conserved. This blend of structural conservation and divergence in the precursors appears to be caused by splice junction sliding and translational frameshift in the 3'-region of the PPP gene [Yonekura et al., J. Biol. Chem. 263 (1988) 2990–2997]. In the present study, we determined the nucleotide (nt) sequences of the chicken PPP (cPPP) cDNA and gene, and compared them with those of the mammals. In cPPP, the C-terminal region of the precursor is quite heterologous with respect to the rat (rPPP) and human (hPPP) precursors, and this heterogeneity is accentuated by the large deletion in exon 3 of cPPP. Furthermore, mutational accumulation during evolution caused the structural organization of the 3'-region of cPPP to change; cPPP is terminated in exon 3, whereas rPPP and hPPP are terminated in exon 4. Thus, our previous observation regarding the possibility of ‘mosaic evolution’ [Yamamoto et al., J. Biol. Chem. 261 (1986) 6156–6159] of PPP has been extended and confirmed by this study. Available evidence suggests that ‘mosaic evolution’ is a phenomenon unique to PPP, and not to the genes encoding the other members of the PPP family, neuropeptide-Y and peptide-YY.  相似文献   

19.
Two-dimensional polyacrylamide gel electrophoresis revealed 10 polymorphic proteins in seed embryos of 29 cultivated rices (Oryza sativa L.) including 16 japonica cultivars, three so-called ‘Javanica’ ones and 10 indica ones. We attempted to use these polymorphic proteins to identify rice subspecies by scoring the polymorphisms. Since all japonica cultivars examined showed the same pattern of protein spots, we considered it to be a standard one with a score of zero, and the protein polymorphisms of other cultivars were given scores of 0.0, 0.5 or 1.0 according to spot density. This scoring method gave characteristic scores for indica and ‘Javanica’ cultivars, i.e. typical japonica cultivars selected as standards presumed the score of 0.0 whereas ‘Javanica’ cultivars and indica ones had the scores of 2.5–4.0 and of 5.0–8.0, respectively. By using this scoring method and the subspecies-specific proteins previously reported, 19 cultivars of unknown subspecies were classified as three indica cultivars and 16 japonica ones including four so-called ‘Javanica’ ones. This scoring method also detected a difference between the perennial wild rice Oryza rufipogon and the annual one O. nivara at the protein level.  相似文献   

20.
Phylogenetic relations among tanaidacean genera within ‘Akanthophoreinae’ are addressed using computer-assisted parsimony methods. The morphology-based analysis includes 10 well-defined and described genera: Araphura, Chauliopleona, Collettea, Paragathotanais, Metagathotanais, Paraleptognathia, Paranarthrura, Portaratrum, and Tanaella in Tanaidomorpha, and Glabroapseudes in Apseudomorpha as the outgroup. Chauliopleona and Paraleptognathia form a monophylum; Portaratrum cannot be placed in any known family. These three genera are considered as incertae sedis. The analysis does not support the monophyly of ‘Akanthophoreinae’, and further questions the monophyly of Tanaellidae.  相似文献   

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