共查询到20条相似文献,搜索用时 31 毫秒
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K U Wagner R J Wall L St-Onge P Gruss A Wynshaw-Boris L Garrett M Li P A Furth L Hennighausen 《Nucleic acids research》1997,25(21):4323-4330
To delete genes specifically from mammary tissue using the Cre-lox system, we have established transgenic mice expressing Cre recombinase under control of the WAP gene promoter and the MMTV LTR. Cre activity in these mice was evaluated by three criteria. First, the tissue distribution of Cre mRNA was analyzed. Second, an adenovirus carrying a reporter gene was used to determine expression at the level of single cells. Third, tissue specificity of Cre activity was determined in a mouse strain carrying a reporter gene. In adult MMTV-Cre mice expression of the transgene was confined to striated ductal cells of the salivary gland and mammary epithelial cells in virgin and lactating mice. Expression of WAP-Cre was only detected in alveolar epithelial cells of mammary tissue during lactation. Analysis of transgenic mice carrying both the MMTV-Cre and the reporter transgenes revealed recombination in every tissue. In contrast, recombination mediated by Cre under control of the WAP gene promoter was largely restricted to the mammary gland but occasionally observed in the brain. These results show that transgenic mice with WAP-Cre but not MMTV-Cre can be used as a powerful tool to study gene function in development and tumorigenesis in the mammary gland. 相似文献
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A mammary cell-specific enhancer in mouse mammary tumor virus DNA is composed of multiple regulatory elements including binding sites for CTF/NFI and a novel transcription factor, mammary cell-activating factor. 总被引:7,自引:3,他引:4 下载免费PDF全文
S Mink E H?rtig P Jennewein W Doppler A C Cato 《Molecular and cellular biology》1992,12(11):4906-4918
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Androgen regulation by the long terminal repeat of mouse mammary tumor virus. 总被引:21,自引:7,他引:14 下载免费PDF全文
Mouse mammary tumor virus (MMTV) has long been implicated in mouse mammary carcinogenesis, and it is now well established that the long terminal repeat (LTR) contains regulatory sequences responsible for glucocorticoid-mediated induction of viral RNA. However, we have demonstrated previously that androgens as well as glucocorticoids can regulate MMTV RNA in the S115 mouse mammary tumor cell line. To determine if androgens act directly on the LTR in these cells, plasmids were constructed with the MMTV LTR joined to the coding sequences of genes not normally expressed in the cells. Following transfection of these chimeric genes into S115 cells, we show that the expression of the genes is regulated by both androgens and glucocorticoids. Furthermore, hormonal regulation is also conferred by the LTR on the neighboring guanine phosphoribosyltransferase (gpt) gene. Thus, androgens can act on the LTR of MMTV when the appropriate receptors are present in the cells, and this interaction can influence the expression of additional adjacent genes. 相似文献
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Glucocorticoid receptors recognize DNA sequences in and around murine mammary tumour virus DNA. 总被引:39,自引:6,他引:33 下载免费PDF全文
S Geisse C Scheidereit H M Westphal N E Hynes B Groner M Beato 《The EMBO journal》1982,1(12):1613-1619