共查询到20条相似文献,搜索用时 46 毫秒
1.
《病毒学报》2016,(2)
详细地阐述了中塞友好生物安全实验室实施埃博拉病毒核酸检测整个质量控制的过程,全面地展示固定P3实验室第一批检测队在埃博拉病毒病实验室检测中表现出的科学、严谨、准确与高效,为实验室检测工作提供参考和依据。首先描述了质控体系要点,包括了组织和人员管理、质量管理文件体系、仪器设备、检测试剂和耗材、审核、实验室空间和功能、实验室维护与安全保障等各个方面的质控要点。其次,从埃博拉病毒核酸检测前、检测中到检测后过程,分述了质控在埃博拉病毒检测整个流程的各个环节中的应用。技术精良的专业检测队伍和实行人性化管理是援非抗埃成功的基石;做好生物安全防护是有效质控和完成检测任务的前提;配备专业的后勤保障确实非常必要;中塞友好生物安全实验室的建立对于中塞友谊具有划时代的意义,该实验室将成为塞拉利昂今后最为重要的埃博拉病毒实验室检测基地。 相似文献
2.
《微生物学免疫学进展》2016,(6)
正目前,埃博拉病毒感染的诊断需将静脉血样运送至生物安全实验室,用实时RT-PCR进行检测,检测周期过长,导致病人病情恶化,感染加剧。因此,对于该病毒感染的诊断迫切需要床前快速检测 相似文献
3.
埃博拉病毒能在人类和非人灵长类中引起严重的埃博拉出血热,病死率可高达90%,并且目前还没有针对埃博拉病毒有效的疫苗或者是治疗手段。面对埃博拉病毒带来的挑战,针对埃博拉病毒的相关研究已经成为病毒学的热点问题。其中,关于埃博拉病毒侵染细胞机制的科学研究对于开发病毒疫苗以及新型治疗药物有非常关键的作用。因此,本综述拟就埃博拉病毒侵染细胞机制方面的研究进展进行总结。 相似文献
4.
埃博拉病毒感染致死率最高可达90%,属烈性传染病,检测诊断对于疫情控制就显得异常重要。目前我国实验室通过实时定量荧光PCR检测埃博拉病毒核酸进行确诊,但耗时相对较长,对检测的人员和仪器要求较高。ELISA法检测血清是病原学诊断的主要指标,该种检测方法简单易操作,能够有效判断患者是否感染埃博拉以及感染程度,可用于流行病学调查,血清学指标的检测可以作为核酸检测的有力补充。由于埃博拉出血热在我国境内尚无病例出现,尤其需要储备血清学检测阳性质控物。通过基因工程抗体技术构建并表达抗埃博拉病毒核蛋白NP人-鼠嵌合抗体,为埃博拉出血热血清学检测做人源阳性对照储备。采用RT-PCR技术,从分泌抗埃博拉核蛋白单克隆抗体的鼠杂交瘤细胞株中分离克隆抗体轻重链可变区基因,对其进行序列分析。根据序列分析的结果,设计特异性引物将鼠源抗体可变区基因VH、VL分别克隆至携带有人抗体轻重链恒定区基因的真核表达载体HL51-14,瞬时转染293T细胞,表达并纯化获得人鼠嵌合IgG抗体,随后对其进行了免疫学检测和功能鉴定。结果表明正确构建抗埃博拉病毒核蛋白人鼠嵌合抗体真核表达载体,成功表达并纯化获得两株人鼠嵌合单抗。 相似文献
5.
高晶晶 《微生物学免疫学进展》2016,(4):25
正目前,埃博拉病毒感染的诊断需将静脉血样运送至生物安全实验室,用实时RT-PCR进行检测,检测周期过长,导致病人病情恶化,感染加剧。因此,对于该病毒感染的诊断迫切需要床前快速检测手段。本文作者报道Corgenix ReEBOV抗原快速检测试剂盒现场验证的结果。作者对塞拉利昂两个临床中心的106名疑似埃博拉病毒感染者的指尖针刺采血进行快速诊断试 相似文献
6.
MicroRNA是一类通过影响RNA表达、抑制蛋白质翻译来调节基因表达的内源性非编码小分子RNA。自从2014年首次发现苏丹型和扎伊尔型埃博拉病毒能够编码microRNA以来,不断有研究发现新的病毒microRNA以及其在埃博拉病毒复制、扩散、免疫逃逸中发挥的作用。宿主microRNA则参与到抗病毒防御机制中,但也面临着病毒蛋白对其调控的阻力包括相应宿主microRNA水平的变化以及RNA沉默抑制子的编码。深入研究埃博拉病毒与宿主在microRNA水平上的动态相互作用,有利于进一步了解埃博拉病毒的致病机制以及开发新的诊断、治疗策略。本文就microRNA在埃博拉病毒感染中的作用做一简要综述。 相似文献
7.
本研究的目的为建立一种灵敏、快速、定量检测埃博拉病毒莱斯顿亚型核酸的实时荧光定量PCR检测方法及试剂盒。首先选择埃博拉病毒莱斯顿亚型的保守基因NP基因作为检测靶目标,筛选出保守序列并设计合成一对特异性引物。然后将连有NP全长基因的重组质粒作为定量标准品,将10倍系列稀释的重组质粒进行荧光定量PCR扩增,绘制标准曲线,并进行重复性、灵敏度及特异性检测。结果显示建立的埃博拉病毒莱斯顿亚型荧光定量PCR检测方法,其灵敏度可达102拷贝/μL,不同梯度标准品间线性关系(R2)达0.997,斜率为-0.3101,扩增效率为110.145%,所有标准品均在79.94℃出现尖且窄的特异性熔解峰。利用该检测系统可以快速定量检测埃博拉病毒莱斯顿亚型核酸,灵敏度高、重复性好,可用于基础及临床实验室对埃博拉病毒莱斯顿亚型感染的快速诊断和临床效果的监测,具有实际的应用价值。 相似文献
8.
9.
10.
11.
12.
13.
14.
Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
15.
16.
RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
17.
JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
18.
肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
19.
20.
H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献