共查询到20条相似文献,搜索用时 15 毫秒
1.
Recent advances in wheat transformation 总被引:9,自引:0,他引:9
Vasant janakiraman Martin Steinau Serena B. McCoy Harold N. Trick 《In vitro cellular & developmental biology. Plant》2002,38(5):404-414
Summary Since the first report of wheat transformation in the early 1990s, genetic engineering of wheat has evolved rapidly. Several
laboratories worldwide have reported the production of fertile transgenic wheat plants using a variety of methods. While there
are several innovative and promising approaches for wheat transformation using different explants as targets for transformation,
different methods of transformation, and different selection schemes, the most common approach to wheat transformation is
the bombardment of tissue derived from immature embryos followed by selection based on resistance to the bar gene. Even with all these successful reports, hurdles still exist for this recalcitrant crop. Of these hurdles, low transformation
rates, tools for transgene expression, and transgene silencing in subsequent generations are probably the most critical. This
review will provide an overview of wheat transformation in the past decade, addressing both positive and negative factors
that effect transformation while highlighting the successes of the past and prospects for the future. 相似文献
2.
Lynn S. Dahleen Muthusamy Manoharan 《In vitro cellular & developmental biology. Plant》2007,43(6):493-506
Barley, an important member of the cereals, has been successfully transformed through various methods such as particle bombardment,
Agrobacterium tumefaciens, DNA uptake, and electroporation. Initially, the transformation in barley concentrated on developing protocols using marker
genes such as gus, bar, and hpt. Immature embryos and callus derived from immature embryos were targeted for transformation. Subsequently, genes of agronomic
and malting importance have been deployed in barley. Particle bombardment appears to be the preferred choice for barley transformation
in the majority of the reports, although Agrobacterium-mediated transformation is being used more often. The current review focuses on the challenges encountered in barley transformation
such as somaclonal variation, development of transformation systems for commercial cultivars, gene expression, stability and
inheritance, and gene flow. Newer markers such as the green fluorescent protein (gfp), firefly luciferase, and phosphomannose isomerase were found to be useful in the selection of transgenic plants. Tissue-specific
promoters such as those for B1-hordein and D-hordein genes, and spike-specific promoters, are increasingly used to drive gene
expression. The review also describes recent research on gene-tagging through transformation, insertion of disease resistance,
and abiotic stress resistance genes, transformation with genes for improved malting quality, nutrient content, feed quality,
and the production of feed enzymes and pharmaceutical compounds. 相似文献
3.
叶绿体遗传转化的研究进展 总被引:1,自引:0,他引:1
核转化技术是基因工程的主要方法,但其多方面的不安全性使人们把焦点转向了植物基因工程另一目标:叶绿体遗传转化。本文介绍了叶绿体基因及基因组;叶绿体遗传转化的原理和方法:叶绿体转化的优点。重点介绍了关于叶绿体遗传转化国内外研究新进展。 相似文献
4.
Recent advances in the genetic transformation of trees 总被引:18,自引:0,他引:18
As the commercial production of transgenic annual crops becomes a reality in many parts of the world, many people wonder if the genetic engineering of perennial trees will allow their eventual commercialization. Not long ago, trees were considered to be recalcitrant material for most molecular biology techniques, including genetic transformation. However, transgenes for shortening the juvenile phase or for phytoremediation purposes have now been incorporated, and the alteration of lignin biosynthesis and increased cellulose accumulation in forest trees have also been accomplished. For long-lived tree species, new questions arise regarding the stability of integration and expression of foreign genes. Biosafety considerations, including transgene dispersion through the pollen and advances in strategies to avoid this, are also important. 相似文献
5.
Summary Forage and turf grasses are critical to sustainable agriculture and contribute extensively to the world economy. Tremendous
progress has been made in genetic transformation of forage and turf grasses in the past decade. The rapid advancement of cellular
and molecular biology and transgenic technology provides novel methods to accelerate and complement conventional breeding
efforts. This review summarizes the latest developments in genetic transformation methods and the applications of molecular
techniques for the improvement of forage and turf grasses. 相似文献
6.
Recent advances in palaeomycology 总被引:1,自引:1,他引:0
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Stott S 《BMJ (Clinical research ed.)》2000,320(7231):358-361
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KORRINGA P 《The Quarterly review of biology》1952,27(4):539-65; concl
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The endosomal network is an organized array of intracellular, membranous compartments that function as sorting sites for endosomal and biosynthetic cargo. The fate of endocytic cargo is reliant upon interactions with a number of molecularly distinct sorting complexes, which tightly control the relationship between sorting of their respective cargo and the physical process of membrane re-scuplturing required for the formation of transport carries. One such complex, retromer, mediates retrograde transport from endosomes to the trans-Golgi network (TGN). Disregulation of retromer has been implicated in a host of disease states including late-onset Alzheimer's. Rather than give a broad overview of retromer biology, here we aim to outline the recent advances in understanding this complex, focussing on the involvement of both clathrin and the cytoskeleton in retromer function. 相似文献
20.
Recent advances in ganglioside metabolism 总被引:1,自引:0,他引:1
G Tettamanti R Ghidoni M Trinchera 《Indian journal of biochemistry & biophysics》1988,25(1-2):106-111