Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review

Microalgae have the ability to mitigate CO₂ emission and produce oil with a high productivity, thereby having the potential for applications in producing the third-generation of biofuels. The key technologies for producing microalgal biofuels include identification of preferable culture conditions for high oil productivity, development of effective and economical microalgae cultivation systems, as well as separation and harvesting of microalgal biomass and oil. This review presents recent advances in microalgal cultivation, photobioreactor design, and harvesting technologies with a focus on microalgal oil (mainly triglycerides) production. The effects of different microalgal metabolisms (i.e., phototrophic, heterotrophic, mixotrophic, and photoheterotrophic growth), cultivation systems (emphasizing the effect of light sources), and biomass harvesting methods (chemical/physical methods) on microalgal biomass and oil production are compared and critically discussed. This review aims to provide useful information to help future development of efficient and commercially viable technology for microalgae-based biodiesel production.     

FRET analysis of domain formation and properties in complex membrane systems

The application of Förster Resonance Energy Transfer (FRET) to the detection and characterization of phase separation in lipid bilayers (both in model systems and in cell membranes) is reviewed. Models describing the rate and efficiency of FRET for both uniform probe distribution and phase separation, and recently reported methods for detection of membrane heterogeneity and determination of phase boundaries, probe partition coefficients and domain size, are presented and critically discussed. Selected recent applications of FRET to one-phase lipid systems, gel/fluid phase separation, liquid ordered/liquid disordered phase separation (lipid rafts), complex systems containing ceramide and cell membranes are presented to illustrate the wealth of information that can be inferred from carefully designed FRET studies of membrane domains.     

Industrial separation of carboxylic and amino acids by liquid membranes: applicability, process considerations, and potential advantage

Liquid-liquid extraction and membrane separation are well-known separation method of extensive industrial application. Their incorporation into liquid membranes has the potential of several advantages, some of which are of particular interest for the recovery of carboxylic and amino acids: selectivities higher than those attainable by current separation methods, saving on energy costs for final concentration of separated products, high fluxes, compact installation, and low capital and operation costs. Stability of the liquid advantages, can be secured by utilizing extractant blocking polymeric membranes, Applicability, process consideration, and economic implications for recovery for carboxylic and amino acids by various extractant/membrane combinations are discussed. (c) 1993 John Wiley & Sons, Inc.     

Protein recovery using gas–liquid dispersions

Two separation techniques, foam separation and colloidal gas aphrons (CGAs), both of which are based on gas–liquid dispersions, are compared as potential applications for protein recovery in downstream processing. The potential advantages of each method are described and the concentration and selectivity achieved with each method, for a range of proteins is discussed. The physical basis of foam separation is the preferential adsorption of surface active species at a gas–liquid interface, with surface inactive species remaining in bulk solution. When a solution containing surface active species is sparged with gas, a foam is produced at the surface: this foam can be collected, and upon collapse contains surface active species in a concentrated form. CGAs are microbubble dispersions (bubble diameters 10–100 μm) with high gas hold ups (>50%) and relatively high stability, which are formed by stirring a surfactant solution at speeds above a critical value (typically around 5000 rpm). It is expected that when proteins are brought into contact with aphrons, protein adsorbs to the surfactant through electrostatic and/or hydrophobic forces. The aphron phase can be separated easily from the bulk solution due to its buoyancy, thus allowing separation of protein in a concentrated form.     

Hydrodynamics and mass transfer of CO2 in water in a tubular photobioreactor

Microalgae cultivation has received growing importance because of its potential applications in CO₂ bio‐fixation, wastewater treatment and biofuel production. In this regard, proper design of photobioreactors is crucial for large‐scale commercial applications. The hydrodynamics of a photobioreactor has great influence on the transfer of CO₂ from gas phase to liquid phase. Considering the facts, the present research focused on studying the gas holdups and mass transfer from the gas to liquid phase in a tubular photobioreactor at various superficial liquid velocities ranging from 8.4 to 22.4 cm/s and superficial gas velocities ranging from 3.66 to 8.1 cm/s. It was found that the gas holdups were radially distributed. The highest gas holdups were observed at the center zone while the lowest holdups are found near the reactor wall. CO₂ mass transfer coefficient in the photobioreactor was also estimated under different superficial liquid velocities (0.206, 0.355 and 0.485 cm/s) and gas velocities (0.67, 1.16 and 1.37 cm/s). The overall mass transfer coefficient was estimated by fitting the experimental data and comparing results with an unsteady state differential mole balance equation solved by Runge‐Kutta‐Gill method. Model predictions were comparable to experimental results.     

Metabolic engineering of Cyanobacteria and microalgae for enhanced production of biofuels and high‐value products

A lot of research has been performed on Cyanobacteria and microalgae with the aim to produce numerous biotechnological products. However, native strains have a few shortcomings, like limitations in cultivation, harvesting and product extraction, which prevents reaching optimal production value at lowest costs. Such limitations require the intervention of genetic engineering to produce strains with superior properties. Promising advancements in the cultivation of Cyanobacteria and microalgae have been achieved by improving photosynthetic efficiency through increasing RuBisCO activity and truncation of light‐harvesting antennae. Genetic engineering has also contributed to final product extraction by inducing autolysis and product secretory systems, to enable direct product recovery without going through costly extraction steps. In this review, we summarize the different enzymes and pathways that have been targeted thus far for improving cultivation aspects, harvesting and product extraction in Cyanobacteria and microalgae. With synthetic biology advancements, genetically engineered strains can be generated to resolve demanding process issues and achieve economic practicality. This comprehensive overview of gene modifications will be useful to researchers in the field to employ on their strains to increase their yields and improve the economic feasibility of the production process.     

Biofuels from microalgae

Microalgae are a diverse group of prokaryotic and eukaryotic photosynthetic microorganisms that grow rapidly due to their simple structure. They can potentially be employed for the production of biofuels in an economically effective and environmentally sustainable manner. Microalgae have been investigated for the production of a number of different biofuels including biodiesel, bio-oil, bio-syngas, and bio-hydrogen. The production of these biofuels can be coupled with flue gas CO2 mitigation, wastewater treatment, and the production of high-value chemicals. Microalgal farming can also be carried out with seawater using marine microalgal species as the producers. Developments in microalgal cultivation and downstream processing (e.g., harvesting, drying, and thermochemical processing) are expected to further enhance the cost-effectiveness of the biofuel from microalgae strategy.     

Harvesting Systems,Soil Cultivation,and Nitrogen Rate Associated with Sugarcane Yield

The adoption of mechanical harvesting of green cane gives rise to concerns as to whether systems developed under burnt cane harvesting are applicable to a green cane harvesting system. In particular, tillage, which is an integral part of the burnt cane system, may no longer be necessary, and the nitrogen fertilizer rates required may need to be replaced due to the large amounts of organic matter being returned to the soil after green cane harvesting. Mechanical harvesting is relatively new in Brazil and little is known about its effect on other sugarcane production strategies. This work aimed to evaluate sugarcane performance under not only different harvesting and cultivation systems, but also different nitrogen fertilizer rates over a 3-year period. The experimental design was a split plot with harvesting systems (burnt vs. green) as main plots, cultivation (interrow vs. no cultivation) as sub plots, and nitrogen rates as sub-sub plots. The harvesting systems produced similar sugarcane yields throughout the experimental period, which demonstrates that the harvest systems do not influence sugarcane yield. Mechanical tillage practices in interrow after harvesting had no impact on stalk yield or sugar quality, indicating no necessity for this operation in the following crop. Ratoon nitrogen fertilization promoted an increase of stalk and sugar yield, with highest yields obtained at the rate of 130 kg ha^?1 N. However, there was no interaction between harvesting system and nitrogen rate.     

Membrane separations using molecularly imprinted polymers

This review presents an overview on the promising field of molecularly imprinted membranes (MIM). The focus is onto the separation of molecules in liquid mixtures via membrane transport selectivity. First, the status of synthetic membranes and membrane separation technology is briefly summarized, emphasizing the need for novel membranes with higher selectivities. Innovative principles for the preparation of membranes with improved or novel functionality include self-assembly or supramolecular aggregation as well as the use of templates. Based on a detailed analysis of the literature, the main established preparation methods for MIM are outlined: simultaneous membrane formation and imprinting, or preparation of imprinted composite membranes. Then, the separation capability of MIM is discussed for two different types, as a function of their barrier structure. Microporous MIM can continuously separate mixtures based on facilitated diffusion of the template, or they can change their permeability in the presence of the template ("gate effect"). Macroporous MIM can be developed towards molecule-specific membrane adsorbers. Emerging further combinations of molecularly imprinted polymers (MIPs), especially MIP nanoparticles or microgels, with membranes and membrane processes are briefly outlined as well. Finally, the application potential for advanced MIM separation technologies is summarized.     

Agave for tequila and biofuels: an economic assessment and potential opportunities

This paper explores the economic viability of producing biofuels from Agave in Mexico and the potential for it to complement the production of tequila or mescal. We focus on Agave varieties currently being used by the tequila industry to produce two beverages, tequila and mescal, and explore the potential for biofuel production from these plants. Without competing directly with beverage production, we discuss the economic costs and benefits of converting Agave by‐products to liquid fuel as an additional value‐added product and expanding cultivation of Agave on available land. We find that the feedstock cost for biofuel from the Agave piña alone could be more than US$3 L^?1 on average. This is considerably higher than the feedstock costs of corn ethanol and sugarcane ethanol. However, there may be potential to reduce these costs with higher conversion efficiencies or by using sugar present in other parts of the plant. The costs of cellulosic biofuels using the biomass from the entire plant could be lower depending on the conversion efficiency of biomass to fuel and the additional costs of harvesting, collecting and transporting that biomass.     

An immobilized cell reactor with simultaneous product separation. II. Experimental reactor performance

The simultaneous separation of volatile fermentation products from product-inhibited fermentations can greatly increase the productivity of a bioreactor by reducing the product concentration in the bioreactor, as well as concentrating the product in an output stream free of cells, substrate, or other feed impurities. The Immobilized Cell Reactor-Separator (ICRS) consists of two column reactors: a cocurrent gas-liquid "enricher" followed by a countercurrent "stripper" The columns are four-phase tubular reactors consisting of (1) an inert gas phase, (2) the liquid fermentation broth, (3) the solid column internal packing, and (4) the immobilized biological catalyst or cells. The application of the ICRS to the ethanol-from-whey-lactose fermentation system has been investigated. Operation in the liquid continuous or bubble flow regime allows a high liquid holdup in the reactor and consequent long and controllable liquid residence time but results in a high gas phase pressure drop over the length of the reactor and low gas flow rates. Operation in the gas continuous regime gives high gas flow rates and low pressure drop but also results in short liquid residence time and incomplete column wetting at low liquid loading rates using conventional gas-liquid column packings. Using cells absorbed to conventional ceramic column packing (0.25-in. Intalox saddles), it was found that a good reaction could be obtained in the liquid continuous mode, but little separation, while in the gas continuous mode there was little reaction but good separation. Using cells sorbed to an absorbant matrix allowed operation in the gas continuous regime with a liquid holdup of up to 30% of the total reactor volume. Good reaction rates and product separation were obtained using this matrix. High reaction rates were obtained due to high density cell loading in the reactor. A dry cell density of up to 92 g/L reactor was obtained in the enricher. The enricher ethanol productivity ranged from 50 to 160 g/L h while the stripper productivity varied from 0 to 32 g/L h at different feed rates and concentrations. A separation efficiency of as high as 98% was obtained from the system.     

Life cycle assessment of natural gas power plants in Thailand

Background, aim, and scope  The main primary energy for electricity in Thailand is natural gas, accounting for 73% of the grid mix. Electricity generation from natural gas combustion is associated with substantial air emissions. The two technologies currently used in Thailand, thermal and combined cycle power plant, have been evaluated for the potential environmental impacts in a “cradle-to-grid” study according to the life cycle assessment (LCA) method. This study evaluates the environmental impacts of each process of the natural gas power production over the entire life cycle and compares two different power plant technologies currently used in Thailand, namely, combined cycle and thermal. Materials and methods  LCA is used as a tool for the assessment of resource consumption and associated impacts generated from utilization of natural gas in power production. The details follow the methodology outlined in ISO 14040. The scope of this research includes natural gas extraction, natural gas separation, natural gas transmission, and natural gas power production. Most of the inventory data have been collected from Thailand, except for the upstream of fuel oil and fuel transmission, which have been computed from Greenhouse gases, Regulated Emissions, and Energy use in Transportation version 1.7 and Global Emission Model for Integrated Systems version 4.3. The impact categories considered are global warming, acidification, photochemical ozone formation, and nutrient enrichment potential (NEP). Results  The comparison reveals that the combined cycle power plant, which has a higher efficiency, performs better than the thermal power plant for global warming potential (GWP), acidification potential (ACP), and photochemical ozone formation potential (POCP), but not for NEP where the thermal power plant is preferable. Discussion  For the thermal power plant, the most significant environmental impacts are from power production followed by upstream of fuel oil, natural gas extraction, separation, and transportation. For the combined cycle power plant, the most significant environmental impacts are from power production followed by natural gas extraction, separation, and transportation. The significant difference between the two types of power production is mainly from the combustion process and feedstock in power plant. Conclusions  The thermal power plant uses a mix of natural gas (56% by energy content) and fuel oil (44% by energy content); whereas, the combined cycle power plant operates primarily on natural gas. The largest contribution to GWP, ACP, and NEP is from power production for both thermal as well as combined cycle power plants. The POCP for the thermal power plant is also from power production; whereas, for combined cycle power plant, it is mainly from transmission of natural gas. Recommendations and perspectives  In this research, we have examined the environmental impact of electricity generation technology between thermal and combined cycle natural gas power plants. This is the overview of the whole life cycle of natural gas power plant, which will help in decision making. The results of this study will be useful for future power plants as natural gas is the major feedstock being promoted in Thailand for power production. Also, these results will be used in further research for comparison with other feedstocks and power production technologies.     

On-land cultivation of functional seaweed products for human usage

Worldwide, there has been much interest in the development and commercialization of human functional products from seaweeds. Novel seaweed compounds with potential applications as bioactive ingredients in natural health products are being isolated in a number of active research programs on this topic. The majority of these research programs do not include cultivation as a critically important component in scaling the discoveries up to commercialization (i.e., economies of scale realized). Many of these seaweeds of interest with potential as functional human products are diminutive in size, sparse in density, and seasonal in occurrence and bioactive efficacy, making commercialization by resource management and harvesting economically challenging and the application of traditional ocean-based production methods risky. Human functional products will require sustainable production coupled with quality assurance and standardized, consistent efficacy. Since humans are the consumers of these types of functional seaweed products, traceability and security of supply are of the utmost importance to successful commercialization. On-land cultivation is essential for commercial success in the development of human functional products from seaweeds at industrial scales. On-land cultivation allows the highest levels of control over quality, efficacy, traceability, and security. On-land cultivation represents the most environmentally acceptable method for the production of biomass from natural resources that could not be economically or sustainably developed any other way. However, on-land cultivation has many associated barriers to development, including high costs associated with capital, operations, maintenance, and cultivar development, and these demands limit industrial scale development of seaweed functional products for human consumption.     

Cultivation of Gigartina skottsbergii (Gigartinales, Rhodophyta): Recent advances and challenges for the future

This study integrates landings statistics and biological studies of the red algaGigartina skottsbergii Setchell & Gardner. The analysis of the landings and carrageenan production in Chile suggeststhat this resource will suffer a strong harvesting pressure during the nextyears. Biological results on sporulation, germination, sporeling growth and survivorship in laboratory,indoor tanks and field conditions, indicated that cultivation of this species istechnically feasible, as spores can be seeded on ropes and other substrata. Vegetative propagation of this species through tissue fragmentationis also possible. Vegetative fragments of this carrageenophyte have 20 to30% higher growth rates than whole fronds in suspended culture systems. Protoplast production can be also explored for bypassing restrictions inspore availability. Major advantages that encourage the cultivation of G. skottsbergii include its gel quantity and quality, its pathogen-freecondition, a high reproduction potential and its regeneration capacity. Onthe other hand, the major constraints are related to its relatively slowgrowth as compared to other carrageenophytes, limited availability ofspores and high mortality during juvenile stages.     

Effects of cultivation years on effective constituent content of Fritillaria pallidiflora Schernk

Fritillaria pallidiflora Schrenk has been treasured in traditional classic medicine as an antitussive, antiasthmatic and expectorant for hundreds of years. With gradually decreasing wild F. pallidiflora resources, the herb can no longer satisfy the demand. Artificial cultivation is one of the most effective ways to solve the contradiction between supply and demand in the medicinal material market. During the growth of Rhizomes medicinal plants, root biomass and active ingredient content showed dynamic accumulated variation with increasing cultivation years. Up to now, hardly any attempts have been made to investigate the relationship between quality and cultivation years of F. pallidiflora. Therefore, in this paper, we determined the optimum harvesting time by comparing biomass and biological characteristics of F. pallidiflora at different cultivation times. High-performance liquid chromatography with evaporative light scattering detection and phenol-sulfuric acid visible spectrophotometry was performed to determine imperialine and polysaccharide content of F. pallidiflora bulbs. From year 1 to 6 of cultivation, we observed an upward trend in plant height, diameter and dry weight of F. pallidiflora, while water content decreased. Plant height and dry weight increased remarkably during the fourth year of cultivation. The content of imperialine and polysaccharide of F. pallidiflora bulbs, on the other hand, showed an upward trend from year 1 to 3, after which it decreased from year 3 to 6. By comparing plant growth, biomass development and the accumulation of imperialine and polysaccharide, the best harvesting time of F. pallidiflora was determined to be after 4 years of cultivation. Our results showed that it is possible to establish a “safe, effective, stable and controllable” production process, which could play an important role in achieving sustainable utilization of F. pallidiflora resources.     

Bacterial farming by the fungus Morchella crassipes

The interactions between bacteria and fungi, the main actors of the soil microbiome, remain poorly studied. Here, we show that the saprotrophic and ectomycorrhizal soil fungus Morchella crassipes acts as a bacterial farmer of Pseudomonas putida, which serves as a model soil bacterium. Farming by M. crassipes consists of bacterial dispersal, bacterial rearing with fungal exudates, as well as harvesting and translocation of bacterial carbon. The different phases were confirmed experimentally using cell counting and ¹³C probing. Common criteria met by other non-human farming systems are also valid for M. crassipes farming, including habitual planting, cultivation and harvesting. Specific traits include delocalization of food production and consumption and separation of roles in the colony (source versus sink areas), which are also found in human agriculture. Our study evidences a hitherto unknown mutualistic association in which bacteria gain through dispersal and rearing, while the fungus gains through the harvesting of an additional carbon source and increased stress resistance of the mycelium. This type of interaction between fungi and bacteria may play a key role in soils.     

Harvesting of Spirulina platensis by cellular flotation and growth stage determination

AIM: To investigate an effective harvesting method for Spirulina platensis. METHODS AND RESULTS: Eighty per cent of S. platensis cells in the logarithmic growth phase were harvested by flotation when the cells were set in a static condition for 2 h. The optimum harvesting time was about day 6 of cultivation. The flotation activity of S. platensis cells was enhanced by the addition of NaCl. CONCLUSIONS: The harvesting of S. platensis by flotation is a cost-effective and straightforward method that can retain the algal quality. The optimum harvesting time of S. platensis can be predicted by the cellular protein to carbon ratio. SIGNIFICANCE AND IMPACT OF THE STUDY: Flotation harvesting is also applicable to other cyanobacteria with gas vesicles.     

Environmental Life Cycle Assessment of a Canned Sardine Product from Portugal

This study aims to assess the environmental impacts of canned sardines in olive oil, by considering fishing, processing, and packaging, using life cycle assessment (LCA) methodology. The case study concerns a product of a canning factory based in Portugal and packed in aluminum cans. It is the first LCA of a processed seafood product made with the traditional canning method. The production of both cans and olive oil are the most important process in the considered impact categories. The production of olives contributes to the high environmental load of olive oil, related to cultivation and harvesting phases. The production of aluminum cans is the most significant process for all impact categories, except ozone depletion potential and eutrophication potential, resulting from the high energy demand and the extraction of raw materials. To compare to other sardine products consumed in Portugal, such as frozen and fresh sardines, transport to the wholesaler and store was added. The environmental cost of canned sardines is almost seven times higher per kilogram of edible product. The main action to optimize the environmental performance of canned sardines is therefore to replace the packaging and diminish the olive oil losses as much as possible. Greenhouse gas emissions are reduced by half when plastic packaging is considered rather than aluminum. Frozen and fresh sardines represent much lower environmental impacts than canned sardines. Nevertheless, when other sardine products are not possible, it becomes feasible to use sardines for human consumption, preventing them from being wasted or used suboptimally as feed.     

Three different examples of enzymatic bioconversion in liquid membrane reactors

Three different examples of enzyme emulsions are presented. The enzymes are immobilized in liquid surfactant membranes. The effect of the organic membrane phase is discussed as well as the influence of the membrane composition on the transport of substrates and products through the membrane. An enzyme emulsion system for the production of l-leucine with continuous co-factor regeneration is described. It is not necessary to increase the molecular weight of the co-factor by linking it to a soluble high molecular weight compound (e.g., PEG), since the coenzyme cannot pass the liquid membrane without a suitable carrier. Also, a product (6-APA) can be enriched in the internal phase of the liquid membrane. The separation effect is not based on differences in molecular weight, but on the chemical behavior of the substances to be separated.     

Variations in the leaf metabolite profile between hydroponic and field grown Moringa oleifera Lam. genotypes

Medicinal and herbal plants are increasingly cultivated on commercial scale all around the world, to satisfy the large demand for natural remedies associated with plant bioactive compounds. Moringa oleifera Lam. is one of these attractive plant species, being cultivated across the world, with most of the production in Africa and Asia. However, it is increasingly reported that the composition and concentration of bioactive compounds in medicinal plants, are largely influenced by factors such as the growing environment, harvesting time, and climatic factors among others. The aim of this study was therefore to determine the differences in the leaf metabolite profile between hydroponic and field grown M. oleifera plants due to cultivation system, harvesting time and genotypes. ¹H NMR was used for data acquisition, and MestReNova software was used for the pre-processing of the spectral data. Multivariate data analysis by means of PCA and OPLS-DA was conducted, followed by targeted metabolite analyses. The results show variations in the leaf metabolite profile as influenced by cultivation systems, harvesting time and genotypes. In both PKM-1 and Malawi hybrid genotypes, the concentration level of chlorogenic acid, ferulic acid, vanillic acid, and wogonin compounds were higher in the hydroponic leaf material, whereas the concentration level of esculetin, niazirin and GABA were slightly higher in the field harvested leaf materials. With regards to harvesting time effect, the concentration level of all seven targeted compounds were higher in February (summer) harvest as compared to May (autumn) harvest. Among the genotypes, PKM-1 showed to have higher concentration of certain targeted compounds as compared to Malawi hybrid, but on the other hand certain compounds from the Malawi hybrid showed also higher concentrations. These results provide insight on the possible alternative production system that can be adopted to optimise and improve accumulation of bioactive compounds in M. oleifera leaf materials.