共查询到20条相似文献,搜索用时 15 毫秒
1.
Motility and orientation of a dinoflagellate, Gymnodinium, impaired by solar and ultraviolet radiation 总被引:1,自引:0,他引:1
Abstract The effects of solar and artificial ultraviolet radiation on the motility and orientation of the dinoflagellate Y-100 were studied. The cells show a weak photokinesis but a pronounced phototaxis which is consistently positive between 1 and 100 klx (= 4 mW m−2 to 400 mW m−2 ); the precision of orientation increases with the fluence rate. Unfiltered solar radiation as well as artificial ultraviolet radiation reduce the percentage of motile cells increasingly with exposure time but the velocity of the still motile cells is less affected. Unirradiated control cells show a negative gravitaxis. After short exposure to solar or artificial ultraviolet radiation the precision of gravitaxis decreases and after prolonged exposure the cells start to actively move downward in the water column (positive gravitaxis). Phototaxis is also strongly impaired by ultraviolet radiation. 相似文献
2.
Gyrodinium dorsum Kofoid responds photophobically to flashes of blue light. The photophobic response consists of a cessation of movement (stop-response). Without background light and after a flash fluence above 10 J m−2 , 75–85% of the cells show a stop-response, while only 50% of the cells show this response at 5 J m−2 . With a flash fluence of 5 J m−2 , background light of different wavelengths either increases (614 nm. 5.5–18.2 μmol m−2 s−1 ) or decreases (700 nm, 18.4–36.0 μmol m−2 s−1 ) the stop-response. Two hypotheses for the mechanism of the modulation by background light of the photophobic response are discussed: an effect of light on the balance of the photosynthetic system (PS I/PS II) or an effect on a phytochrome-like pigment (Pr /Pfr ). This study supports the idea that a phytochrome-like pigment works in combination with a blue light-absorbing pigment. It was also found that cells of Gyrodinium dorsum cultured in red light (39.8 μmol m−2 ) had a higher absorption in the red region of the absorption spectra than those cultured in white light (92.7 μmol m−2 ). 相似文献
3.
Maize ( Zea mays L. hybrid ZP-704) and black pine ( Pinus nigra Arn.) were grown for five days at low fluence rate (0.4–4.0, μmol m–2 s−1 ) in blue or red light. Compared to red light of the same fluence rate, blue light effects in maize were repressive for the accumulation of Chita, b , carotenoids and light-harvesting complex-2 (LHC-2) proteins. The maximal reduction of proteins bound to the light-harvesting complex of photosystem 2 and pigments was attained at different fluence rate levels. In black pine, blue light compared to the red of the same fluence rate level either activated or reduced accumulation of pigments and LHC proteins, the effect being dependent on its fluence rate level. At fluence less than 3.0 μmol m−2 s−1 blue light was more efficient for the synthesis of Chi a, b and carotenoids, hut for LHC-2 complexes, fluence rates between 0.4 and 1.5 [μmol m−2 s−1 were more effective. In pine the effects of the two lights on the accumulation of pigments and LHC proteins were demonstrated separately and were dependent on fluence rate level. This suggests irradianoe-controlled activation/deactivation of the photoreceptor at the level of the cell. 相似文献
4.
Physiological effects of long-term exposure to low and moderate concentrations of atmospheric NH3 on poplar leaves 总被引:2,自引:0,他引:2
L. W. A. VAN HOVE O. VAN KOOTEN E. H. ADEMA W. J. VREDENBERG G. A. PIETERS 《Plant, cell & environment》1989,12(9):899-908
Abstract. Poplar shoots ( Populus euramericana L.) obtained from cuttings were exposed for 6 or 8 weeks to NH3 concentrations of 50 and 100 μgm−3 or filtered air in fumigation chambers. After this exposure the rates of NH3 uptake, transpiration, CO2 assimilation and respiration of leaves were measured using a leaf chamber. During the long-term exposure also modulated chlorophyll fluorescence measurements were carried out to obtain information about the photosynthetic performance of individual leaves. Both fluorescence and leaf chamber measurements showed a higher photosynthetic activity of leaves exposed to 100 μg NH3 m−3 . These leaves showed also a larger leaf conductance and a larger uptake rate of NH3 than leaves exposed to 50 μg m−3 NH3 or filtered air. The long-term NH3 exposure did not induce an internal resistance against NH3 transport in the leaf, nor did it affect the leaf cuticle. So, not only at a short time exposure, but also at a long-term exposure NH3 uptake into leaves can be calculated from data on the boundary layer and stomatal resistance for H2 O and ambient NH3 -concentration. Furthermore, the NH3 exposure had no effect on the relation between CO2 -assimilation and stomatal conductance, indicating that NH3 in concentrations up to 100 μg m−3 has no direct effect on stomatal behaviour; for example, by affecting the guard or contiguous cells of the stomata. 相似文献
5.
Relative effectiveness and interaction of ultraviolet-B, red and blue light in anthocyanin synthesis of apple fruit 总被引:3,自引:0,他引:3
The effect of light on anthocyanin production in apple ( Malus pumila Mill. cv. Jonathan) skin disks was investigated, with prolonged irradiation from different light sources. High fluence rates of white light provided from a xenon lamp were unable to produce large amounts of anthocyanin, and anthocyanin production became saturated at about 30 W m−2 . When UV-B light, provided by a fluorescent lamp which had an emission peak at 312 nm, was combined with the white light, anthocyanin production was synergistically stimulated and increased up to the highest fluence rates of white light tested (44 W m−2 ). This UV-B light was more effective than red and blue light provided from fluorescent lamps, but anthocyanin production became saturated at about 1.7 W m−2 . However, simultaneous irradiation with red and UV-B light had a synergistic effect. UV-B light was also effective in increasing anthocyanin production in whole fruit. Therefore this synergism seemed to have an important role in the development of the desirable red skin color under field light conditions. The results of aminoethoxyvinylglycine treatment suggested that ethylene was not involved in the stimulative effect of UV-B light. 相似文献
6.
Stefano Predieri Donald T. Krizek Chien Y. Wang Roman M. Mirecki Richard H. Zimmerman 《Physiologia plantarum》1993,87(2):109-117
In vitro shoots of cv. Doyenne ďHiver pear ( Pyrus communis L.) were irradiated under controlled environments for 6 h per day at 5 different levels of biologically effective UV-B radiation (UV-BBE ). UV-B exposure caused a progressive increase in apical necrosis above background levels and stimulated leaf abscission. Shoots grown for 2 weeks at 7. 8 mol m−2 day −1 of photosynthetic photon flux (PPF) and treated with 8. 4 or 12. 0 kJ m−2 day −1 UV-BBE produced up to 4 times more ethylene than those given 2. 2 or 5. 1 kJ m−2 day−1 UV-BBE or untreated controls. Exposure of shoots to 12 kJ m−2 day −1 of UV-BBE caused an increase in free putreseine content after 4 to 14 days of irradiation. Shoots showed a decrease in CO2 uptake after 3 days of UV-B: thereafter, they appeared to recover their photosynthetic capacity. Under typical PPF conditions used in micropropagation (90 μmol m−2 S−1 ). 8. 4 kJ m−2 day −1 of UV-B radiation was injurious to realatively tender tissues of in vitro pear shoots: increasing the level of UV-BBE to 12 kJ m−2 day−1 produced even more adverse effects. 相似文献
7.
Mitsuru Dei 《Physiologia plantarum》1984,62(4):521-526
Excised etiolated cotyledons of cucumber ( Cucumis sativus L. cv. Aonagajibai) were continuously irradiated under various intensities of white light. The rate of chlorophyII (Chi) formation during the lag phase reaches a plateau at fluence rates above 1.4 urmol m−2 s−1 . This is true in both water-control and benzyladenine (BA)-pretreated cotyledons. In cotyledons pretreated for 14 h with BA in darkness (in which case, Chl formation is stimulated by BA during both the lag and the steady-state phases), the increase in the steady-state rate of Chl formation with increasing light in tensity is stimulated compared to that of the water control over the range of fluence rates, 0. 25-43 urmol m−2 s−1 . In cotyledons pretreated for 6 h with BA in darkness (only Chl formation during the lag phase is stimulated), only an increase in fluence rate from 0.25 to 1.4 umol m−2 s−1 causes a higher increase in the Chl formation in the BA-treated cotyledons than in the water control. The time course of Chl formation shows that the BA-induced late-appearing effect (stimulation of the steady-state rate) is almost absent at low intensity illumination, but the BA-induced fast-appearing effect (elimination of the lag phase) is effective at all intensities. From this evidence, the Chl-forming process apparently consists of two components, whose periods of operation or light-intensity requirements are different. BA stimulates the rates of the respective components in both the fast and the late-appearing effects. 相似文献
8.
Tomato plants were grown under light intensities of 36 or 90 W m−2 [photosynthetically active radiation (PAR)], and then the light intensity was changed to 36, 90 or 180 W m−2 for 8 h to investigate the effect of temporary changes in light intensity on the carbon budget of photoassimilates from the third leaf using a 14 CO2 steady-state feeding method. In the plants that were raised under 90 W m−2 , the photosynthetic rate increased when the light intensity was increased to 180 W m−2 , whereas no increase occurred in the plants that were raised under 36 W m−2 . Although the total amount of carbon fixed during the 8-h light period showed a large difference between plants grown at the two initial light intensities, the proportion of carbon exported during the light period did not differ apparently, irrespective of the change in light intensity. However, the amount of carbon exported during the time course was higher in plants that were raised under 90 W m−2 than those raised under 36 W m−2 , irrespective of the change in light intensity. The partitioning pattern of 14 C-photoassimilates was not changed by the change in light intensity, irrespective of whether the light intensity was increased or not. However, the amount of 14 C-photoassimilates accumulated in each part differed according to the two initial light intensities. The carbon transport from a source leaf was also investigated through a quantitative analysis of carbon balance. 相似文献
9.
In darkness, sporalation of Penicillium claviforme Bainier CBS strain 126–23 was uniform. A single 10 J m−2 blue light pulse (in the range 360 to 520 nm) was sufficient to elicit an endogenous zonation rhythm (coremia formation); the higher the fluence, the longer the rhythm expression. The period was 30 to 36 h as long as sufficient fluence rate (3 μW m−2 for broad band blue light and 18.1 nmol m−2 s−1 for wavelengths 433, 457 or 465 nm) was continuously maintained; this rhythm became desynchronized in about a week. The period increased rapidly and reached 72 to 120 h as soon as the fluence rate was too low or dark was established. The 445,479 and 495 nm radiations evoked the rhythm in pulse experiments, whereas the rhythm was immediately desynchronized in continuous light. The participation of two photosensitive reactions in the rhythm regulation of P. claviforme is postulated. 相似文献
10.
Hiroshi Fukayama Naotsugu Uchida Tetsushi Azuma Takeshi Yasuda 《Physiologia plantarum》1998,104(4):541-548
The effects of the ratio of Rubisco activase to Rubisco (activase/Rubisco ratio) on light dependent activation of CO2 assimilation were investigated during leaf aging of rice. Changes of photosynthetic CO2 gas exchange rates in relation to step increases of light intensity from two photon flux densities of 60 µmol m−2 s−1 (low initial PFD) and 500 µmol m−2 s−1 (high initial PFD) to saturated PFD of 1 800 µmol m−2 s−1 were measured. These photosynthetic activation processes were considered to be limited by the Rubisco activation rate when analyzed by the relaxation method. The relaxation time of low initial PFD gradually declined from 3 to 33 days after leaf emergence and showed high and negative correlation to the activase/Rubisco ratio. The initial rate of Rubisco activation under low initial PFD linearly correlated to the amounts of Rubisco activase, whereas these were almost constant from 3 to 23 days after leaf emergence. But these correlations could not be recognized in the case of high initial PFD. Moreover, the relaxation times were more sensitive to intercellular CO2 concentration (Ci ) under high initial PFD than under low initial PFD, especially, at Ci below 300 µl l−1 . These results suggest the involvement of the activase/Rubisco ratio in the photosynthetic activation under relatively low initial PFD, and the limitation of photosynthetic activation under relatively high initial PFD by Rubisco carbamylation during leaf aging of rice. 相似文献
11.
Influence of solar ultraviolet radiation on photosynthesis and motility of marine phytoplankton 总被引:2,自引:0,他引:2
Abstract: The effects of solar ultraviolet radiation (UV) on carbon uptake, oxygen evolution and motility of marine phytoplankton were investigated in coastal waters at Kristineberg Marine Research Station on the west coast of Sweden (58° 30'N, 11° 30'E). The mean irradiances at noon above the water surface during the investigation period were: photosynthetic active radiation (PAR, 400–700 nm) 1670 μmol m−2 s−1 ; ultraviolet-A radiation (UV-A, 320–400 nm) 35.9 W m−2 and ultraviolet-B radiation (UV-B, 280–320 nm) 1.7 W m−2 . UV-B radiation was much more attenuated with depth in the water column than were PAR and UV-A radiation. UV-B radiation could not be detected at depths greater than 100–150 cm. Inhibition of carbon uptake by UV-A and UV-B in natural phytoplankton populations was greatest at 50 cm depth and the effects of UV-B were greater than those of UV-A. At depths greater than 50 cm there was almost no effect of ultraviolet radiation on carbon uptake. PAR, UV-A and UV-B decreased oxygen evolution by the dinoflagellate Prorocentrum minimum . Inhibition of oxygen evolution was greater after 4 h than 2 h but it was not possible to distinguish the negative effects of the different light regimes. The motility of P. minimum was not affected by PAR, UV-A and UV-B. The importance of exposure of phytoplankton to different light regimes before being exposed to natural solar radiation is discussed. 相似文献
12.
Proliferating cultures of Actinidia deliciosa A. Chev., C. F. Liang and A. R. Ferguson cv. Tomuri (♂) were grown under photosynthetic photon flux density (PPFD) rates ranging from 30 to 250 μmol m−2 s−1 in order to determine certain physiological parameters in vitro: CO2 evolution, photosynthesis at three CO2 atmospheric concentrations (330, 1450 and 4500 μl l−1 ), fresh and dry matter accumulation and proliferation rate.
A proportional response in dry weight, dry/fresh weight ratios and PPFD was found. The proliferation rate increased up to 120 μmol m−2 s−1 but decreased at higher rates. At the highest PPFD, the CO2 released from cultures and accumulated in the vessels reached 200 μl l−1 of; at the lowest rate the CO2 concentration reached 10500 μl l−1 after 28 days of culture. The photosynthetic rate at 1450 and 4500 μl l−1 of CO2 was nearly 4 times higher than at the lowest concentration tested. 相似文献
A proportional response in dry weight, dry/fresh weight ratios and PPFD was found. The proliferation rate increased up to 120 μmol m
13.
The effects of photon flux density and temperature on net photosynthesis and transpiration rates of mature and immature leaves of three-year-old Japanese larch Larix kaempferi (Lamb.) Sarg. trees were determined with an infrared, differential open gas analysis system. Net photosynthetic response to increasing photon flux densities was similar for different foliage positions and stage of maturity. Light compensation was between 25 and 50 μmol m−2 s−1 . Rates of photosynthesis increased rapidly at photon flux densities above the compensation level and became saturated between 800 and 1000 μmol m−2 s−1 . Transpiration rates at constant temperature likewise increased with increasing photon flux density, and leveled off between 800 and 1000 μmol m−2 s−1 . Photosynthetic response to temperature was determined in saturating light and was similar for all foliage positions; it increased steadily from low temperatures to an optimum range betweeen 15 and 21°C and then decreased rapidly above 21°C. Transpiration rate, however, increased continuously with rising temperature up to the experimental maximum. CO2 compensation concentrations for mature foliage varied between 58 and 59 μl l−1 ; however, foliage borne at the apex of the terminal leader compensated at 75 μl l−1 . None of these data support the claim that Japanese larch possesses C4 photosynthetic characteristics. 相似文献
14.
An experiment was conducted on intact algal assemblages of stream periphyton to test their response to fluctuating and constant light regimes having the same mean intensity. The light regimes (in μmol·m−2 ·s−1 ) were constant light at 100, light fluctuating between 50 and 150 with a period of 5 min, and light fluctuating between 10 and 460 with periods of either 4:1 or 8:2 min. Compared to the rates measured under 100 in μmol·m−2 ·s−1 constant light conditions, fluctuations ranging between 50 and 150 in μmol·m−2 ·s−1 with a 5-min period produced a 23% greater rate of photosynthesis. Conversely, fluctuations between 10 and 460 in μmol·m−2 ·s−1 led to a 59%–74% decrease in photosynthetic activity. Detailed examination of periphytic algal responses to fluctuating light revealed that higher light intensities produced steeper photosynthesis/time slopes, but it was the combined interaction with lower light intensity that ultimately determined overall photosynthetic rate for a given light regime. This study offers compelling evidence that variable light regimes have important consequences for algal photosynthesis in natural streams. 相似文献
15.
Characterization of a rice mutant having an increased susceptibility to light stress at high temperature 总被引:2,自引:0,他引:2
The effect of light irradiation at high temperature was investigated in a rice mutant, spl-2 , which is sensitive to solar radiation. Dead spots appeared on the mutant leaves when cultured at a high temperature (40°C) under strong white-light illumination (15 W m−2 ). A similar damage was also observed in the wild-type leaves under the same conditions when the plants were preincubated in the dark for one day. Preillumination with weak light (6 W m−2 ) lessened the irradiation-induced damage in the wild-type. These observations suggest that in rice plants the acclimatization to weak light has a protective effect against strong irradiation at high temperature, and the spl-2 locus participates in the mechanisms of the acclimatization. The action spectrum for the irradiation-induced damage at the high temperature (40°C) in the spl-2 leaves indicated that the maximum damage occurred at around 480 nm and, in a lesser extent, at around 680 nm. Activity of the O− 2− and H2 O2− scavenging enzymes in the spl−2 leaves were almost the same or somewhat higher than those in the wild-type after irradiation with strong white light (15 W m−2 ) at 40°C, whereas the content of ascorbic acid in the spl−2 decreased significantly compared with the wild type. 相似文献
16.
The regulation of hypocotyl extension in light-grown Chenopodium rubrum L. seedlings by light analogous to dense vegetation canopy shade has been monitored. Hypocotyl extension was controlled by both the quantity and quality of the actinic light. At the higher of the two background photon fluence rates which were used (10.0 μmol m−2 s−1 in the 400–700 nm waveband), increasing the proportion of phytochrome calculated to exist as Pfr resulted in greater inhibition of growth. At the lower photon fluence rate (1.0 μmol m−2 s−1 in the 400–700 nm waveband), a biphasic response was observed in which minimum inhibition was observed at intermediate photoequilibria. Although photosynthesis was not directly involved in the photomorphogenetic responses, it did play an indirect quantitative role in determining the response. 相似文献
17.
Mitsuru Dei 《Physiologia plantarum》1985,64(2):153-160
Benzyladenine (BA) stimulated 5-aminolevulinic acid (ALA) accumulation in the presence of levulinic acid during illumination with 43 μmol m−2 s−1 light in excised etiolated cotyledons of cucumber ( Cucumis sativus L. cv. Aonagajibai). A short dark-pretreatment (6 h) with BA eliminated the lag phase of ALA accumulation. The rate of ALA accumulation during the steady-state phase in cotyledons pretreated with BA for a long period (14 h) was considerably accelerated compared to that in cotyledons pretreated with BA for 6 h. The rate of ALA accumulation during the lag phase was saturated at a very low light fluence (<1.4 μmol m−2 s−1 ) in both BA-pretreated and water-control cotyledons. The steady-state rate of ALA accumulation increased with increasing light fluence up to 43 μmol m−2 s−1 (parallel to that of Chl formation) in water-control cotyledons. In contrast, in cotyledons pretreated with BA for either 6 or 14 h, the steady-state rate reached a plateau at a very low light fluence. Based on the above results together with our finding that there are two components of Chl formation (M. Dei, 1984. Physiol. Plant. 62: 521–526) possible intermediate steps of Chl biosynthesis pathway affected by BA and light intensity are discussed. 相似文献
18.
The present study investigates the light acclimation potential of photoautotrophic suspension culture cells of Chenopodium rubrum L. grown in 16 h light/8 h dark cycles. Typical features of sun/shade acclimation could be demonstrated in cultures grown at photon flux densities of 30 and 150 μmol m−2 s−1 . Low light grown cells had lower chlorophyll a/b ratios, lower respiration rates and lower light compensation points than high light grown cells. Maximum photosynthetic rate per cell dry weight was highest in low light conditions, indicating that the cells did not enlarge their photosynthetic machinery upon exposure to high light. Transfer of cultures to 800 μmol m−2 s−1 caused photoinhibition as indicated by a decrease in photosynthetic efficiency and by the occurrence of a slowly reversible quenching of variable chlorophyll fluorescence. Extension of the photoinhibitory treatment over six light dark cycles did not result in further dramatic changes of these parameters, whereas the chlorophyll content per dry weight and the chlorophyll a/b ratio decreased. Measurements of photochemical quenching showed that the capability of the cells to dissipate excessive energy had increased during the acclimation process. The presence of the xanthophyll cycle pigments and the operation of the cycle could be demonstrated. In agreement with the putative photoprotective function of antheraxanthin and zeaxanthin these pigments could only be detected under photoinhibitory conditions. Prolonged photoinhibitory treatment resulted in increases in the xanthophyll pigment concentration but not of the potential to deepoxidate violaxanthin. The limited potential of the cells to accumulate zeaxanthin and antheraxanthin might indicate that the xanthophyll cycle is not the main factor determining their resistance to high light stress. 相似文献
19.
JÜRG FUHRER 《Plant, cell & environment》1982,5(4):263-270
Abstract. Seedlings of Phaseolus vulgaris were exposed to solutions containing Cd2+ in the range 0 to 1 molm−3 . Ethylene formation started following 3 h of exposure to 10−2 , 10−1 and 1 mol m−3 Cd2+ , peaked at 18 h and returned to a relatively low rate after 24 h. Cadmium-induced ethylene formation depended on the formation of 1-aminocyclopropane-1-carboxylic acid (ACC). Aminoethoxyvinylglycine (AVG, 0.1 mol m−3 ) inhibited ACC accumulation and ethylene production during exposure to 0.2 mol m−3 Cd2+ .
Activity of soluble and ionically-bound peroxidase increased after 18 h of exposure to Cd2+ concentrations above 10−3 mol m−3 due to an increase in activity of cathodic isoperoxidases. Stimulation of soluble and ionically-bound peroxidase by 0.2 mol m−3 Cd2+ was reduced in the presence of 0.1 mol m−3 AVG.
Accumulation of soluble and insoluble ('ligninlike') phenolics was found in plants exposed to Cd2+ (10−2 mol m−3 or above) in the presence or absence of AVG. Deposition of insoluble (autofluorescing) material occurred in cell walls around vessels and was associated with reduced expansion and water content of leaves. 相似文献
Activity of soluble and ionically-bound peroxidase increased after 18 h of exposure to Cd
Accumulation of soluble and insoluble ('ligninlike') phenolics was found in plants exposed to Cd
20.
Cyanophora paradoxa Korshikov synchronized autotrophically in a light-dark regime of 14 h light and 10 h dark divides in the last two hours of the dark period. The division rate of the free-living blue-green alga, Synechococcus leopoliensis Raciborski, at identical culture conditions (24°C; 32 W m−2 ) is only slightly lowered in the light period. The comparison of thylakoid differentiation in the endocyanelles of Cyanophora paradoxa and in Synechococcus leopoliensis during the light-dark regime yields (1) the same ensemble of pigment-protein complexes in both organisms, (2) comparable syntheses of chlorophyll and phycobilins of Cyanophora paradoxa grown under 32 W m−2 and of Synechococcus leopoliensis grown under light intensities below 9.2 W m−2 , and (3) identical photosynthetic oxygen evolution during the light period of the light-dark regime with minima at the beginning, in the middle (6th–7th h), and at the end of the light period. In both organisms this stage-specific oxygen evolution is inhibited by treatment with chloroamphenicol. Cycloheximide, however, causes no significant alterations. Results are discussed in view of the endosymbiotic theory. 相似文献