Review of bees as guests in termite nests,with a new record of the communal bee, <Emphasis Type="Italic">Gaesochira obscura</Emphasis> (Smith, 1879) (Hymenoptera,Apidae), in nests of <Emphasis Type="Italic">Anoplotermes banksi</Emphasis> Emerson, 1925 (Isoptera,Termitidae, Apicotermitinae)

Associations between bees and termites are documented infrequently, but records are available for bee species ranging in behavior from solitary to highly eusocial. The subtribe Meliponina (stingless bees) is the most common bee group reported in termite nests, and, for some species, the occupancy of termite nests may be obligatory. The records of solitary bees nesting within termite nests include species of the tribes Emphorini, Centridini, Megachilini, and Paracolletini. Most of these bees can probably nest in other substrates, and their relationships with termite nests are presumably opportunistic. This study provides a review of published records of bees as guests in termite nests, and also describes the aggregation of nests of Gaesochira obscura within one nest of Anoplotermes banksi in Brazilian Amazonia. One termite nest contained at least ten nest entrances of G. obscura, with burrows 4–6 mm in diameter and about 10 cm long. Each burrow ended in brood cells in different stages of food provisioning and larval development. As commonly reported for other associations of this nature, there was no connection between the tunnels of bees and those of termites. This record adds important data on the biology of A. banksi. Because this is a single record, it is impossible to classify G. obscura either as a termitophile or termitariophile; this species may be opportunistic in relation to nesting substrate.     

Farmers’ perception of termites in agriculture production and their indigenous utilization in Northwest Benin

Background

Although termites are considered as agricultural pests, they play an important role in maintaining the ecosystem. Therefore, it matters to investigate the farmers’ perception of the impacts of the termites on the agriculture and their indigenous utilization.

Methods

A semi-structured questionnaire was used to interview 94 farmers through 10 villages of Atacora department, in the northwestern region of Benin, to obtain information for the development of successful strategies of termite management and conservation. Their perceptions on the importance and management of termites along with the indigenous nomenclature and utilization of termite mounds were assessed. Termite species identified by farmers were collected and preserved in 80% alcohol for identification.

Results

Eight crops were identified by farmers as susceptible to termites with maize, sorghum, and yam as being the most susceptible. According to farmers, the susceptibility to termites of these crops is due to their high-water content and sweet taste. A total of 27 vernacular names of termites were recorded corresponding to 10 species, Amitermes evuncifer, Macrotermes subhyalinus, and Trinervitermes oeconomus being the most damaging termite species. All the names given to termite species had a meaning. The drought was identified by farmers as the main factor favouring termite attacks. Demolition of termite mounds in the fields was the most commonly reported control method. Salt and other pesticides were commonly used by farmers to protect stored farm products. The lack of effective control methods is the main constraint for termite management. In northwestern Benin, farmers reported different purpose utilizations of termite mounds and termites.

Conclusions

The study has shown that farmers perceived termites as pests of several agricultural crops and apply various indigenous control practices whose efficiency need to be verified. Utilization of termites and termite mound soil as food and medicinal resources underlines the need for a more focused approach to termite control for the conservation of non-pest termite species. The sensitization of farmers on the importance of termites as well as the development of an integrated control method to combat termite pests proved necessary.

     

The Predation Strategy of the Recluse Spider <Emphasis Type="Italic">Loxosceles rufipes</Emphasis> (Lucas, 1834) against four Prey Species

Generalist predators have to deal with prey with sometimes very different morphologies and defensive behaviors. Therefore, such predators are expected to express plasticity in their predation strategy. Here we investigated the predatory behavior of the recluse spider Loxosceles rufipes (Araneae, Sicariidae) when attacking prey with different morphologies and defensive mechanisms. We expected L. rufipes to show different prey capture strategies and variable acceptance towards each prey type. Potential prey species were collected directly from the web or in the surroundings of the web-building site of L. rufipes. We collected and used the following in our experiments: termite workers (Nasutitermes sp.), lepidopteran larvae (Eurema salome), ants (Camponotus sp.) and isopods (Tylidae). We paired these prey with L. rufipes and recorded their behavior in captivity, quantifying acceptance rate, immobilization time and the sequence of behaviors by the predator. The acceptance rate was lower for isopods but not different among other prey. The immobilization time was higher for isopods than for termites and similar for the other pairwise comparisons. The behavioral sequence was similar for all prey except for isopods, which were also bit more often. Our combined results show plasticity in the behavior of L. rufipes and also show it subdues a potentially dangerous prey (ant) and an armored prey (isopod).     

The Trojan horse approach for managing invasive ants: a study with Asian needle ants, <Emphasis Type="Italic">Pachycondyla chinensis</Emphasis>

Ants are among the most ecologically and economically significant biological invaders and are notoriously difficult to eradicate once established. Invasive ants are typically managed with toxic baits which are often unattractive to the target species, toxic to non-targets, and environmentally persistent. The current study evaluated a novel Trojan horse approach for managing invasive ants in natural habitats based on the use of poisoned prey. Eastern subterranean termites (Reticulitermes flavipes) were topically exposed to fipronil and presented to Asian needle ants (Pachycondyla chinensis) which are a significant invader in natural and disturbed habitats in the eastern United States. In laboratory assays, P. chinensis colonies were offered fipronil-treated termites within experimental arenas. The termites were readily attacked and consumed and results demonstrate that a single termite exposed to 25 ppm fipronil for 1 h is capable of killing 100 P. chinensis workers in 9 h. To evaluate population effects, field studies were conducted in forested areas invaded by P. chinensis. Fipronil-treated termites scattered on the forest floor provided rapid control of P. chinensis and ant densities throughout the treated plots declined by 98 ± 5 % within 28 days. I demonstrate that the poison baiting approach based on fipronil-treated termite prey is highly effective against P. chinensis and may offer an effective alternative to traditional bait treatments against other invasive ants, especially those with predatory and generalist feeding habits. Furthermore, I demonstrate that the poison baiting approach offers environmental benefits by delivering substantially less toxicant to the environment relative to current control methods which rely on commercial bait formulations. In summary, the poison baiting approach evaluated in this study appears highly suitable for controlling invasive ants and should be further tested against other invasive ants.     

The ecology of <Emphasis Type="Italic">Altrichthys azurelineatus</Emphasis> and <Emphasis Type="Italic">A. curatus,</Emphasis> two damselfishes that lack a pelagic larval phase

Out of more than 380 species of damselfish, only three lack a pelagic larval phase, Acanthochromis polyacanthus, that ranges from the Philippines to the Great Barrier Reef, and two species in the genus Altrichthys, A. azurelineatus and A. curatus, that are restricted to the Calamian Archipelago (Palawan province) in the Philippines. Therefore in a very restricted geographic area, northern Palawan, Philippines, all three species co-occur. The goal of this study was to fill a knowledge gap about the ecology of Altrichthys, where very little is currently known. Using census and behavioral approaches at 14 sites on six islands, we were able to identify adult densities, nest densities, nesting habitat preference, and brood behavior and abundance. We found that densities were very high (more than 17 individuals/100 m² for one species) and A. azurelineatus may be displacing A. curatus at deeper depths where habitat seems of higher quality. Only one out of 14 surveyed sites contained only one species, Altrichthys curatus. Different habitat use and feeding behavior suggest that niche partitioning may play an important role in the life history of these sympatric species.     

Environmental and biological determinants of <Emphasis Type="Italic">Termitomyces</Emphasis> species seasonal fructification in central and southern Côte d’Ivoire

The Macrotermitinae subfamily is characterized by its symbiosis with fungi of the genus Termitomyces. The most common and presumably primitive mode of reproduction for these fungi is to produce basidiocarps on the mounds of the host termite colony. The seasonal fructification pattern of the fungi seems to depend on the habitat type and termite ecology. We examined Termitomyces diversity and distribution in two phytogeographic zones in central and southern Côte d’Ivoire. Data were collected in different habitats in exhaustive searches with standardized methods for termites and basidiocarps as well. The respective findings were complemented with behavioral and life cycle data of the associated termite species. Basidiocarps occur species-specifically either during the long or the short rainy season in wooded habitats. The diversity and abundance of termites and their associated basidiocarps were significantly correlated only with woody plant species richness. Nuptial flight of termites, comb biomass and Termitomyces fructification periods were correlated. Termitomyces appear to fructify during (1) the period of strong precipitation, (2) in habitats with appropriate microclimatic characteristics.     

Taxonomic revisions in the Microstromatales: two new yeast species,two new genera,and validation of <Emphasis Type="Italic">Jaminaea</Emphasis> and two <Emphasis Type="Italic">Sympodiomycopsis</Emphasis> species

Environmental sampling yielded two yeast species belonging to Microstromatales (Exobasidiomycetes, Ustilaginomycotina). The first species was collected from a leaf phylloplane infected by the rust fungus Coleosporium plumeriae, and represents a new species in the genus Jaminaea, for which the name Jaminaea rosea sp. nov. is proposed. The second species was isolated from air on 50% glucose media and is most similar to Microstroma phylloplanum. However, our phylogenetic analyses reveal that species currently placed in Microstroma are not monophyletic, and M. phylloplanum, M. juglandis and M. albiziae are not related to the type species of this genus, M. album. Thus, Pseudomicrostroma gen. nov. is proposed to accommodate the following species: P. glucosiphilum sp. nov., P. phylloplanum comb. nov. and P. juglandis comb. nov. We also propose Parajaminaea gen. nov. to accommodate P. albizii comb. nov. and P. phylloscopi sp. nov. based on phylogenetic analyses that show these are not congeneric with Jaminaea or Microstroma. In addition, we validate the genus Jaminaea, its respective species and two species of Sympodiomycopsis and provide a new combination, Microstroma bacarum comb. nov., for the anamorphic yeast Rhodotorula bacarum. Our results illustrate non-monophyly of Quambalariaceae and Microstromataceae as currently circumscribed. Taxonomy of Microstroma and the Microstromataceae is reviewed and discussed. Finally, analyses of all available small subunit rDNA sequences for Jaminaea species show that J. angkorensis is the only known species that possess a group I intron in this locus, once considered a potential feature indicating the basal placement of this genus in Microstromatales.     

Chemical and molecular identification of the invasive termite <Emphasis Type="Italic">Zootermopsis nevadensis</Emphasis> (Isoptera: Archotermopsidae) in Japan

Numerous termite species have been introduced outside their native ranges by human transport, and some have become invasive. The dampwood termite Zootermopsis nevadensis (Hagen), which is native to western North America, has been introduced to and become established in Kawanishi City, Hyogo Prefecture, Japan. Zootermopsis nevadensis is subdivided into two subspecies based on cuticular hydrocarbon (CHC) phenotypes: Z. nevadensis nevadensis and Z. nevadensis nuttingi (Haverty and Thorne). Here, we identified Z. nevadensis in Japan as hybrids between the two subspecies. Chemical analysis showed the presence of 7,15-dimethylhenicosane and 5,17-dimethylhenicosane in the CHCs of Z. nevadensis in Japan, corresponding to the CHC phenotype of Z. n. nevadensis. Conversely, all mitochondrial cytochrome c oxidase subunit I sequences of Z. nevadensis in Japan were identical to sequences from Z. n. nuttingi and hybrids between the two subspecies from a native hybrid zone in California, USA. In addition, phylogenetic analysis showed that Z. nevadensis in Japan formed a clade with Z. n. nuttingi and hybrids between the two subspecies. Our results show discordance between the chemical and genetic features of Z. nevadensis in Japan, indicating that individuals of Z. nevadensis in Japan are hybrids between the two subspecies.     

Phylogenetic assessment and taxonomic revision of <Emphasis Type="Italic">Mariannaea</Emphasis>

Four new species of Mariannaea were described in this paper, namely M. chlamydospora, M. cinerea, M. fusiformis, and M. lignicola. Mariannaea chlamydospora is characterized by its cream-colored, zonate colonies on PDA, smooth conidiophores, fusiform conidia, and abundant chlamydospores. Mariannaea cinerea forms grey colonies and ellipsoidal to subglobose conidia. Mariannaea fusiformis forms purple colonies and fusiform to subglobose conidia. Mariannaea lignicola has brown conidiophores and broad hyphae. The molecular phylogeny was inferred using ITS, LSU, and TUB-2 loci. The type species of Mariannaea (M. elegans) is epitypified. The variety M. elegans var. punicea is raised to species rank. Mariannaea clavispora is excluded from Mariannaea because of its cylindrical phialides, straight conidial chains and deviating phylogenetic affinity. Mariannaea nipponica did not fit well the generic concept of Mariannaea based on their morphological characters, and its generic placement remains uncertain. A key to the currently accepted 15 species of Mariannaea is provided.     

Phylogenetic relationship and time of divergence of <Emphasis Type="Italic">Mus terricolor</Emphasis> with reference to other <Emphasis Type="Italic">Mus</Emphasis> species

Mitochondrial DNA control region of Mus terricolor, three aboriginal species M. spretus, M. macedonicus, M. spicilegus; the Asian lineage M. caroli, M. cervicolor, M. cookii; and the two house mice, M. musculus domesticus and M. m. castaneus were analysed to estimate the substitution rate, phylogenetic relationship and the probable time of divergence. Results showed that M. spretus, M. caroli and M. terricolor are highly diverged from each other (caroli/terricolor = 0.146, caroli/spretus = 0.147 and terricolor/spretus = 0.122), whereas M. spretus showed less divergence with two house mice species (0.070 and 0.071). Sequence divergence between M. terricolor and the Palearctic group were found to be ranging from 0.121 to 0.134. Phylogenetic analysis by minimum evolution, neighbour-joining, unweighed pair group method with arithmetic mean and maximum parsimony showed almost similar topology. Two major clusters were found, one included the Asian lineage, M. caroli, M. cookii and M. cervicolor and the other included the house mice M. m. domesticus, M. m. castaneus and the aboriginal mice M. macedonicus and M. spicilegus along with M. spretus, forming the Palearctic clade. M. terricolor was positioned between the Palearctic and Asian clades. Results showed that Palearctic-terricolor and the Asian lineages diverged 5.47 million years ago (Mya), while M. terricolor had split around 4.63 Mya from their ancestor. M. cervicolor, M. cookii and M. caroli diverged between 4.70 and 3.36 Mya, which indicates that M. terricolor and the Asian lineages evolved simultaneously. M. spretus is expected to have diverged nearly 2.9 Mya from their most recent common ancestor.     

Palaearctic species of the <Emphasis Type="Italic">Microchelonus retusus</Emphasis> group (Hymenoptera,Braconidae, Cheloninae)

Microchelonus species of the M. retusus group differ from the other members of the subgenus Microchelonus s. str. (characterized primarily by the 16-segmented female antennae and deepened apical abdominal opening of the male) in their elongate carapace of female abdomen more strongly narrowed apically than toward base. The first key to 45 species of this group, including 7 new species, is given: M. alexeevi Tobias, 1986 (apicalis Alexeev, 1971); M. angustiventris Tobias, 1986; M. apicalis Papp, 1971; M. arnoldii (Tobias, 1964); M. artus Tobias, 1986; M. cisapicalis Tobias, 1989; M. crassitarsus Tobias, 1989; M. dolosus Tobias, 1989; M. elenae Tobias, 1995; M. erosus Herrich-Schaeffer, 1838 (analipennis Fahringer, 1934; hungaricus Szépligeti, 1896; frivalaldszkyi Shenefelt, 1973); M. heraticus Tobias, 1985; M. hofferi Tobias et Lozan, 2006; M. jonaitisi Tobias, 2000; M. justus Tobias, 1989; M. kievorum sp. n. (Ukraine); M. kiritshenkoi (Tobias, 1976); M. klugei Tobias, 2001; M. kopetdagicus (Tobias, 1966) (caucasicus Abdinbekova, 1967, syn. n.); M. korinthiacus sp. n. (Greece); M. kozlovi (Tobias, 1961); M. longirimosus Tobias, 1995; M. madridi sp. n. (Spain); M. marshakovi Tobias, 1986; M. mediterraneus sp. n. (Greece); M. microphthalmus (Wesmael, 1838) (dilatus Papp, 1971); M. mikhaili Tobias, 1989; M. mirabilis (Tobias, 1972); M. morrocanus sp. n. (Morocco); M. nachitshevanicus (Abdinbekova, 1971); M. ononicus Tobias, 2000; M. pamiricus (Voinovskaya-Kriger, 1928); M. retrusus Tobias, 1989; M. retusus (Nees, 1813) (caudatus Thomson, 1874); M. stenogaster Tobias, 1995; M. sternaus (Tobias, 1964); M. subcaudatus (Tobias, 1971); M. subjustus sp. n. (Spain); M. sulcatus Jurine, 1908 (rimulosus Thomson, 1874; rimatus Szépligeti, 1896); M. tersakkanicus Tobias, 2001; M. tjanshanicus Tobias, 1995; M. turcius sp. n. (Turkey); M. volgensis Tobias, 1986; M. xenia Tobias, 2000; M. zorkuli Tobias, 1991.     

South African mouse shrews (<Emphasis Type="Italic">Myosorex</Emphasis>) feel the heat: using species distribution models (SDMs) and IUCN Red List criteria to flag extinction risks due to climate change

Five species of mouse or forest shrews (Myosorex) are endemic to South Africa, Lesotho and Swaziland, four of which (Myosorex varius, Myosorex cafer, Myosorex longicaudatus and Myosorex cf. tenuis) are associated with montane or temperate grassland, fynbos and/or forest habitats while a fifth (Myosorex sclateri) is associated with lowland subtropical forests. Due to their small size, specialised habitat, low dispersal capacity, high metabolism and sensitivity to temperature extremes, we predicted that, particularly for montane species, future climate change should have a negative impact on area of occupancy (AOO) and ultimately extinction risks. Species distribution models (SDMs) indicated general declines in AOO of three species by 2050 under the A1b and A2 climate change scenarios (M. cafer, M. varius, M. longicaudatus) while two species (M. sclateri and M. cf. tenuis) remained unchanged (assuming no dispersal) or increased their AOO (assuming dispersal). While temperate species such as M. varius appear to be limited by temperature maxima (preferring cooler temperatures), the subtropical species M. sclateri appears to be limited by temperature minima (preferring warmer temperatures). Evidence for declines in AOO informed the uplisting (to a higher category of threat) of the Red List status of four Myosorex species to either vulnerable or endangered as part of a separate regional International Union for Conservation of Nature (IUCN) Red List assessment.     

Morphological variation in pollen grains of <Emphasis Type="Italic">Mucuna</Emphasis> (Leguminosae): new biogeographic and evolutionary patterns

Mucuna comprises 105 species with an overall pantropical distribution and is divided into three subgenera: M. subg. Mucuna, M. subg. Stizolobium and M. subg. Macrocarpa. Although phylogenetic studies have supported the occurrence of three main clades, evolutionary relationships among them are not fully resolved. The objective of this study was to examine pollen grain morphology from representatives of all three subgenera and map these onto the phylogenetic trees generated by analysis of other characters. Pollen grain surface, form, size, and aperture number were compared. A Bayesian inference tree using matK sequences was constructed. The results indicate that the representatives of M. subg. Macrocarpa have the smallest pollen grains in the genus (a synapomorphic character here identified for this subgenus) and that species of subgenus Mucuna (those with umbelliform inflorescences) have the largest pollen grains. Additional morphological diversity of the pollen grain surface was noted: reticulate and/or micro-reticulate (in all three subgenera), perforate, gemmate or verrucose (only in M. subg. Mucuna). For all studied taxa, the pollen grains are triaperturate, except for two species of M. subg. Mucuna, which have tetraperturate pollen. The phylogenetic tree obtained using the matK marker resolved M. subg. Stizolobium as the earliest diverging lineage in Mucuna. Based on this phylogeny, a reticulate ornamentation pattern of the pollen surface may represent the ancestral state for the genus, while the larger pollen size and the foraminate, gemmate, and verrucose ornamentations are derived characteristics within the genus. These putative derived ornamentations have been observed only in neotropical species.     

Molecular evidence for a natural diploid hybrid between <Emphasis Type="Italic">Miscanthus</Emphasis><Emphasis Type="Italic">sinensis</Emphasis> (Poaceae) and <Emphasis Type="Italic">M. sacchariflorus</Emphasis>

The hybrid origin of Miscanthus purpurascens has previously been proposed, primarily because of its intermediate morphology. In this study, phylogenies based on the DNA sequences from the internal transcribed spacer region of nuclear ribosomal DNA (nrDNA ITS), on the DNA sequences of the trnL intron and trnL-F intergenic spacer of chloroplast DNA, and on amplified fragment length polymorphism (AFLP) fingerprinting confirm that M. purpurascens originated through homoploid hybridization between M. sinensis and M. sacchariflorus. Two different types of ITS sequences were identified from almost all plants of M. purpurascens. One type was found to be closely related to M. sinensis and the other to M. sacchariflorus. Miscanthus purpurascens was found to possess many M. sinensis- and M. sacchariflorus-specific AFLP bands but no band specific to itself. Clustering with the Unweighted Pair Group Method with Arithmetic Mean and principal coordinate analysis based on the AFLP data also demonstrated that M. purpurascens is an approximate intermediate of the two species. In addition, M. purpurascens has the plastid genome of M. sinensis or M. sacchariflorus, suggesting that either species could be its maternal parent. All specimens of M. purpurascens and its coexisting parental species are identified as diploids (2n = 2x = 38). Possible mechanisms of natural hybridization, hybrid status, chloroplast DNA recombination, and evolutionary implications of this hybridization are also discussed.     

Vetch aphid, <Emphasis Type="Italic">Megoura crassicauda</Emphasis> (Hemiptera: Aphididae), parasitism does not reduce the bean production of narrow-leaved vetch, <Emphasis Type="Italic">Vicia sativa</Emphasis> subsp. <Emphasis Type="Italic">nigra</Emphasis> (Fabaceae)

Megoura crassicauda Mordvilko (Hemiptera: Aphididae) is a dominant aphid species found on Vicia sativa subsp. nigra (L.) Ehrh. (Fabaceae) in the spring. Worker ants of Formica japonica, the dominant ant species attracted to the extrafloral nectaries of V. s. nigra, often attack ladybirds (Coccinella septempunctata), which are aphid enemies. However, the workers of F. japonica do not attack or exclude M. crassicauda, the non-myrmecophilous aphid. It appears that the “bodyguard” retained by the plant guards the plant’s herbivore by attacking the herbivores’ enemies, rather than guarding the plant itself. The relationship between V. s. nigra and M. crassicauda was observed in the field to examine and evaluate the cost of parasitism. Parasitism by M. crassicauda delayed flower bud formation markedly in V. s. nigra but did not kill the plants. V. s. nigra plants that were parasitized showed a net bean production similar to that of the non-parasitized controls. The parasitism rate of M. crassicauda increased when extrafloral nectaries were used by F. japonica. These results may indicate that M. crassicauda provides V. s. nigra with benefits by preventing other serious disadvantages.     

Revision of the genus <Emphasis Type="Italic">Melamphaes</Emphasis> (Melamphaidae): 6. oligo-raker species: <Emphasis Type="Italic">M. papavereus,M. simus,M. hubbsi</Emphasis>

Three oligo-raker species (≤19 rakers on the first gill arch) of the genus Melamphaes have been considered. A new species, M. papavereus, out of the group “M. typhlops” has been described from the Bay of Bengal of the Indian Ocean. M. simus and M. hubbsi out of the group “M. simus” have been revised. M. simus inhabits all oceans between 40° N and 40° S. M. hubbsi has been known from single specimens caught in the central part of the South Atlantic between 11° and 19° S.     

Cork-warts on leaves of Lauraceae: confirming a suspicion

Some studies have reported the presence of leaf cork-warts in several families of plants; however, in Lauraceae, these structures have not been confirmed. Therefore, based on studies of leaf anatomy, we have herein documented the presence of leaf cork-warts in Mezilaurus species of Lauraceae. To accomplish this, we investigated fifteen species from all six genera of the Mezilaurus group, including Anaueria, Chlorocardium, Clinostemon, Mezilaurus, Sextonia, and Williamodendron. Analyses of leaf anatomy were performed through optical and scanning electron microscopy. Our results revealed cork-warts in six species of the genus Mezilaurus: M. crassiramea, M. navalium, M. revolutifolia, M. subcordata, M. synandra, and M. vanderwerffii. In the investigated species M. crassiramea and M. navalium, cork-warts originated from the stomatal complex, from epidermal cells covering oil cells located in mesophyll, and from epidermal cells around trichomes. Therefore, cork-warts could be considered an additional taxonomic character to delimit Mezilaurus species.