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1.
利用分子标记定位农垦58S的光敏核不育基因 总被引:17,自引:0,他引:17
对农垦58S(Oryzasativasp.japonica)/大黑矮生标记基因系FL2组合组建可育集团和不育集团,并以亲本为对照进行了RFLP、RAPD和双引物RAPD分析,结果第12染色体上的一个单拷贝标记G2140与光敏核不育基因连锁遗传,二者间的遗传图距为14.1cM(centimorgan)。在筛选过的1040个随机单引物和190个双引物中,仅引物OPAU10扩增出与光敏核不育基因连锁的1.5kbDNA片段,回收、克隆该DNA片段并制备探针,将其转换成共显性的RFLP标记并命名为OPAU101500。分离群体连锁分析表明该标记与标记G2140紧密连锁,将农垦58S的一对光敏核不育基因定位于第12染色体上。 相似文献
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哺乳动物性别分化调控的分子机制的研究特别是性别分化的层次调控、剂量补偿和性染色体进化这三个领域,已取得快速进展。已经发现Y染色体性别决定区基因(SRY)、X染色体DSS-AHC决定区基因1(DAX-1)、甾类生成因子1基因(SF1)和Wilms瘤抑制基因(WT-1)等与哺乳动物性别决定有关。SRY启动睾丸分化,但胚胎发育成雄性的其余步骤由事丸分泌的激素控制。DAX-1且编码一种女性特异功能的蛋白质,它在男性中被SRY所抑制。SF-1和WT-1在SRY开启之前作用于性腺和肾上腺发育的启动。哺乳动物通过随机失活雌性两条X染色体中的一条来使X连锁的基因在两性间的表达水平达到平衡(剂量补偿)。X染色体失活由X染色体失活中心(XIC)控制。失活的X染色体专一转录基因(XIST)是XIC的强烈候选者,它可能参与X失活的启动。对有袋目和单孔目动物性染色体的研究为我们提供了其进化的信息。有证据支持性染色体起源于一对同源常染色体,而SRY的祖先基因可能是SOX-3。 相似文献
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SOX基因家族的研究现状 总被引:3,自引:2,他引:3
SOX基因家族是一个新发现的基因家族,其主要特征是具有一个保守基序———HMG -box,可以和DNA进行序列特异性的结合。该家族的第一个成员是哺乳动物的性别决定基因SRY/Sry。由于SOX基因在胚胎发育及性别分化过程中可能起着重要的作用,对其研究进展十分迅速。本文根据现有资料总结了有关方面的研究成果。1SOX基因的发现人类中存在一些性反转患者 (XX男性或XY女性 ),其性别表型与其性染色体组成不一致。详尽分析其染色体结构,发现前者大多获得了一段Y片段;后者往往失去了一段Y染色体片段,由于不同患者… 相似文献
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与小麦白粉病抗性基因Pm2紧密连锁PAPD标记的筛选研究 总被引:5,自引:0,他引:5
以256个随机引物对含小麦抗白粉病基因Pm2近等基因系进行RAPD分析,发现17个随机引物的扩增产物在抗、感NILs材料间表现多态性,且其中5个引物经4次以上重复,均获相同结果,其多态性标记分别为OPM081600、OPI041700、OPH19900及OPM16850。当以这5个随机引物对14个已知含Pm2基因的抗病材料及9个不含Pm2基因的感病材料进行检测时,只有标记OPI041700在12个 相似文献
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SRY和SRY盒基因比较树 总被引:4,自引:2,他引:2
性别决定区Y(SRY)基因是人类和哺乳动物睾丸决定因子(TDF)的最佳候选基因。本文基于SRY/Sry和SRY盒基因保守区氨基酸序列相似性,采用聚类分析方法,将该基因家族聚类为四个亚族,即SOXS1,SOXS2,SOXS3和SOXS4,各亚族间同源性小于60%。所有哺乳动物和人类SRY/Sry都聚在SOXS1亚族内。该亚族由SOXS11和SOXS12两组组成,真兽亚纲哺乳动物和人类SRY/Sry基因都集中在SOXS12组内。 相似文献
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转OMT/PGH基因猪外源基因整合及遗传特性研究 总被引:3,自引:0,他引:3
实验以转OMT/PGH基因猪的G0,G1,G2和G3代共8头猪为材料,应用同位素和非同位素标记的染色体原位杂交技术,对外源OMT/PGH基因在猪染色体上整合位点进行研究,结果表明:(1)外源基因可以整合在染色体上,整合的位点是随机的。(2)整合在染色体上的外源基因可以遗传给子代;(3)整合在染色体上的外源基因在转基因动物世代过程中整合的位点是相对稳定的。 相似文献
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一种利用Taq酶快速标记DNA探针的方法 总被引:1,自引:0,他引:1
目的:探索低成本、高效、快速的DNA探针标记方法。方法:以特定引物和16nler的随机引物作为延伸引物,利用Taq酶标记DNA探针。以大肠杆菌Klenow片断随机引物延伸标记法作为对照。点杂交方法检测探针标记效果。结果与结论:Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果,且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。Taq酶标记法是一种低成本、高效、快速的DNA探针标记方法。 相似文献
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Franck Pellestor Anne Girardet Genevive Lefort Brigitte Andro Jean Paul Charlieu 《Molecular reproduction and development》1995,40(3):333-337
Direct in situ labeling of human spermatozoa was performed using the PRINS method. This technique is based on annealing of specific oligonucleotide primers, and subsequent primer extension by a Taq DNA polymerase. The reaction was carried out on a programmable temperature cycler, and labeling was obtained in a 1-hr reaction. The method was successfully tested with specific primers for chromosomes 13, 16, and 21. This suggests that PRINS may be a fast and reliable technique for detecting aneuploidies. © 1995 Wiley-Liss Inc. 相似文献
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Assessment of aneuploidy for chromosomes 8, 9, 13, 16, and 21 in human sperm by using primed in situ labeling technique. 总被引:4,自引:0,他引:4
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F. Pellestor A. Girardet L. Coignet B. Andro J. P. Charlieu 《American journal of human genetics》1996,58(4):797-802
The incidence of aneuploidy was estimated for chromosomes 8, 9, 13, 16, and 21 in mature human spermatozoa by primed in situ (PRINS) labeling technique. This method allows us to perform a chromosome-specific detection by in situ annealing of a centromeric specific primer. A dual color PRINS protocol was adapted to human sperm. The decondensation and the denaturation of sperm nuclei were simultaneously performed by 3-M NaOH treatment. Double labeling of spermatozoa was obtained in <2 h. A total of 96,292 sperm nuclei were analyzed by two independent observers. The estimates of disomy were 0.31% for chromosome 8, 0.28% for chromosome 9, 0.28% for chromosome 13, 0.26% for chromosome 16, and 0.32% for chromosome 21. These homogeneous findings suggest an equal distribution of aneuploidies among autosomal chromosomes in males. 相似文献
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M. Menke J. Fuchs I. Schubert 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(8):1314-1320
The resolution of the chromosomal positions of six high- and one low-copy sequences by oligonucleotide-primed in situ (PRINS)
labelling was compared with corresponding data obtained after fluorescent in situ hybridization (FISH) on field-bean and barley
chromosomes. While PRINS proved to be suitable for the rapid detection of high-copy tandem repeats at the same loci as those
revealed by FISH, no clear PRINS signal was obtained for the low-copy family of vicilin genes at their locus on field-bean
chromosome II. This indicates that localization of short target sequences by primer extension via Taq polymerase in situ does not yet provide a resolution equal, or superior, to FISH on plant chromosomes. Therefore, the use
of a cocktail of chromosome-specific single-copy sequences as primers for PRINS is no alternative for the not as yet feasible
chromosome painting in plants.
Received: 21 April 1998 / Accepted: 12 May 1998 相似文献
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The spatial relationship between the families of repetitive DNAs present at the centromeres of human chromosomes and the position of the kinetochore was examined by combining immunocytochemistry with the PRINS oligonucleotide primer extension technique. Heterochromatic domains were decondensed with 5-azacytidine to facilitate this study. Using this approach our results clearly show that the alphoid DNA sequences are closely associated with the kinetochore of human chromosomes. Simple-sequence satellite DNAs occupy separate, non-overlapping domains within the centromere. These two major families are separated by a third, relatively low-copy repetitive DNA family, SAU-3A. Pulse-field gel electrophoresis was employed to analyse the centromeric domain of human chromosome no. 9 in more detail and the results although preliminary support the conclusions drawn from the immunocytochemistry/PRINS approach.by W.C. Earnshaw 相似文献
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Primed in situ labeling: sensitivity and specificity for detection of alpha-satellite DNA in the centromere regions of chromosomes 13 and 21 总被引:4,自引:0,他引:4
The centromeric alpha-satellite DNA subfamilies from chromosomes 13 and 21 are almost identical in sequence. So far it has proven difficult to discriminate between sequence variations in the chromosome 13 and 21 alpha-satellite regions using in situ techniques. To analyze whether the method of modified single-color and double-color PRINS could be used to detect single nucleotide polymorphisms within this region, we used previously published primers D13Z and D21Z that differ in the terminal 3'-nucleotide and an additionally constructed primer "D13/21-test" lacking the final nucleotide at the 3' end. The results show that a one-base pair mismatch at the 3' end is sufficient to be detected by PRINS. Surprisingly, only about 35% of our samples exhibited the expected combination of two chromosomes 13 specifically labeled with only primer D13Z and two chromosomes 21 specifically labeled with only primer D21Z. The rest of the samples showed a polymorphic distribution of the target sequence for the primers, therefore these primers are not suited for routine detection of chromosomes 13 and 21 during interphase. Our data indicate that an interchromosomal exchange of alpha-satellite DNA takes place between chromosomes 13 and 21, possibly due to a concerted evolution process. 相似文献
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Wallis MC Delbridge ML Pask AJ Alsop AE Grutzner F O'Brien PC Rens W Ferguson-Smith MA Graves JA 《Cytogenetic and genome research》2007,116(3):232-234
In the absence of an SRY orthologue the platypus sex determining gene is unknown, so genes in the human testis determining pathway are of particular interest as candidates. SOX9 is an attractive choice because SOX9 deletions cause male-to-female sex reversal in humans and mice, and SOX9 duplications cause female-to-male sex reversal. We have localized platypus SOX9, as well as the related SOX10, to platypus chromosomes 15 and 10, respectively, the first assignments to these platypus chromosomes, and the first comparative mapping markers from human chromosomes 17 and 22. The autosomal localization of platypus SOX9 in this study contradicts the hypothesis that SOX9 acts as the sex determining switch in platypus. 相似文献
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In the current paper we described the application of primed in situ (PRINS) labeling approach for the chromosomal mapping of repetitive DNA sequences in Danube salmon (Hucho hucho) (2n = 82, NF = 112). PRINS was successfully performed with primers enabling amplification of 5S rRNA genes (minor rDNAs), NOR building DNA sequences (major rDNAs), and telomeric sequences. Two loci of 5S rRNA were observed on distinct chromosome pairs; the minor arrays were located interstitially on the long (q) arms of two large metacentrics (chromosomes No. 3) and the large clusters of 5S rDNAs were assigned to the short (p) arms of two subtelocentric chromosomes No. 18. Major rDNA clusters were observed on the p-arms of two submeta-subtelocentric chromosomes No. 10. These chromosomal areas were built with GC-rich chromatin what was proved in the course of chromomycin A(3) (CMA(3)) staining performed sequentially. Major and minor rDNA families were not co-localized in the Danube salmon chromosomes.The distinct hybridization signals at the ends of all the chromosomes were provided in the course of PRINS with (CCCTAA)( n ) primer. The chromosomal localization of rRNA genes and telomeric DNA sequences was discussed in the context of Salmonidae karyotype evolution. 相似文献
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Kuroiwa A Uchikawa M Kamachi Y Kondoh H Nishida-Umehara C Masabanda J Griffin DK Matsuda Y 《Cytogenetic and genome research》2002,98(2-3):189-193
Chromosome locations of the eight SOX family genes, SOX1, SOX2, SOX3, SOX5, SOX9, SOX10, SOX14 and SOX21, were determined in the chicken by fluorescence in situ hybridization. The SOX1 and SOX21 genes were localized to chicken chromosome 1q3.1-->q3.2, SOX5 to chromosome 1p1.6-->p1.4, SOX10 to chromosome 1p1.6, and SOX3 to chromosome 4p1.2-->p1.1. The SOX2 and SOX14 genes were shown to be linked to chromosome 9 using two-colored FISH and chromosome painting, and the SOX9 gene was assigned to a pair of microchromosomes. These results suggest that these SOX genes form at least three clusters on chicken chromosomes. The seven SOX genes, SOX1, SOX2, SOX3, SOX5, SOX10, SOX14 and SOX21 were localized to chromosome segments with homologies to human chromosomes, indicating that the chromosome locations of SOX family genes are highly conserved between chicken and human. 相似文献
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