共查询到20条相似文献,搜索用时 15 毫秒
1.
R. Landolt S.M. Shaw R.J. Vetter Shu-Hsia Chen 《Prostaglandins & other lipid mediators》1974,5(3):305-312
The use of grain density autoradiography was evaluated as a means of quantifying the distribution of injected prostaglandin E1 and/or its metabolites in the mouse kidney. Fifty microcuries of 3H-PGE1 were injected into each of three mice which were sacrificed at 20 min, 40 min and 45 h after injection. Sections of frozen kidneys were mounted on prepared liquid emulsion slides. Following appropriate exposure and processing, the radioactivity in various regions was quantified by grain counting using an eyepiece grid superimposed over the field at 1000X. The highest grain density was found over the papilla and the second highest grain density occurred at the region of the inner zone of the cortex. In addition, the percentage decrease of grain density from tissue taken 40 min after injection when compared to that from tissue taken 20 min after injection implied that these two regions retained the radioactivity more so than the outer zone of the cortex, the outer zone of the medulla and the glomeruli. 相似文献
2.
The overall efficiency of autoradiography with tritium-labeled thymidine has been found to be influenced by the following conditions: (1) exposure in an atmosphere of CO2 and the use of the stripping-film technique, both of which increase the autoradiographic efficiency when compared to exposure in air or to dip-coating technique; (2) latent image fading, which increases with increasing exposure. Up to 2 wk of exposure, however, this disadvantage is counterbalanced by the fading of the mechanical background produced during stripping or dip-coating; (3) the thickness of the inert coating interposed between the labeled locus and the sensitized emulsion. A layer of inert coating can be obtained that will arrest all beta particles from tritium, while having no effect on more energetic emitters like C14; (4) the amount of tritiated thymidine given, with relatively large amounts producing an increase in the mean grain count per labeled cell but not in the percentage of cells identifiable as labeled; and (5) the type of fixative and the staining procedure used. Feulgen stain reduces the mean grain count in cells labeled with radioactive thymidine, while fixation with acetic alcohol (1:3) reduces the grain count in cells labeled with precursors of ribonucleic acid. 相似文献
3.
Essentially identical smears can be prepared from a pooled suspension of Ehrlich ascites tumor cells labeled with tritiated thymidine. In these smears, the percentage of labeled cells and the mean grain count per labeled cell vary within narrow limits: in the same slide, within 2 to 5% of the mean of the slide. In smears processed together in the same manner, the percentage of labeled cells (calculated on 1,000 cells) varies within 2%, and the mean grain count per labeled cell (calculated on 50 cells) between 15 and 20% of the mean of all smears. Once the limits of variability are known, smears from the same cell population can be used to ensure the accuracy of procedures performed on separate batches of radio-autographs that have been processed separately but in a presumably identical manner. A comparison of the mean grain counts of standard smears from separate batches will indicate if variations, not otherwise detectable by the experimenter, have actually occurred during the radioautographic procedure. 相似文献
4.
LIPID SYNTHESIS, INTRACELLULAR TRANSPORT, STORAGE, AND SECRETION : I Electron Microscopic Radioautographic Study of Liver after Injection of Tritiated Palmitate or Glycerol in Fasted and Ethanol-Treated Rats 总被引:32,自引:22,他引:10
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A time sequence study of intracellular movement of labeled lipid in the liver was carried out on fasted and ethanol-treated rats injected with either palmitate-3H or glycerol-3H by electron microscopic radioautography. The elimination of water-soluble lipid precursors during specimen preparation was checked and found to be complete. The labeled lipid product in the tissue was identified as mostly triglyceride. A dehydration procedure was adapted to minimize the loss of lipid during specimen preparation. At 2 min after injection, the earliest time interval studied, both precursors were found to have penetrated the liver cells, and the label was found over both rough and smooth elements of the endoplasmic reticulum, which is the site of glyceride esterification. From 5 min on, in fasted and especially in ethanol-treated rats, the label was seen also over lipid droplets 0.5–2.0 µ in diameter, which represent "storage lipid" (slowly turning over compartment). Mitochondria became labeled mostly at later time intervals after injection. From 10 min on, concentration of label was seen over the Golgi apparatus, containing small osmiophilic particles. Association of label with groups of particles in smooth-surfaced vesicles and vacuoles in and near the Golgi apparatus and in the vicinity of the sinusoidal border was seen, both after palmitate-3H and glycerol-3H. It is proposed that these particles represent lipoproteins which are formed in the endoplasmic reticulum, "processed" in the Golgi apparatus, and transported in vacuoles to the sinusoid surface to be discharged into the circulation. 相似文献
5.
The experimental data have been analyzed on the labeled cell distribution related to the grain count over the nucleus in autographs of histological sections (5 mcm) made of the rat brain subependymal zone and of epididymis duct epithelium at different time after 3H-thymidine injection and X-irradiation in the dose of 300 cGr. These results served some additional grounds to the recently established conclusion that a repeated successive decrease in the rate of DNA synthesis is occurring in the cell system starting from stem cell (level I) to semistem cell (levels II-VII) (Gracheva, 1982 r). 5 days after irradiation, at the peak of reparative proliferation, the cell reproduction was intensified, these cells having normally both middle and high levels of DNA synthesis. This process is running of the background of the inhibition of reproduction of cells with the inherent low level of DNA synthesis which are to start differentiation after mitosis. All this makes for the increase in the mean grain count over the nuclei, without changes of the inherent rates of DNA synthesis in the successive generations of the stem cells. 相似文献
6.
Autoradiographic studies of the synthesis of RNA and protein as a function of cell volume in Streptococcus faecium 总被引:4,自引:3,他引:1
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Mid-exponential-phase cultures were either labeled continuously with tritiated leucine and uracil or pulse-labeled with tritiated leucine. The amount of leucine and uracil incorporated into protein or RNA per cell was determined by grain counts of autoradiographs of cells seen in electron micrographs; the volume of each cell was determined by three-dimensional reconstruction. The average number of autoradiographic grains around cells continuously labeled with uracil and leucine increased linearly with cell volume. In contrast, while the average grain count around cells pulse-labeled with leucine increased in a near-linear fashion over most of the volume classes, less than the expected number of grains were seen around cells in large- and small-size classes. The distribution of grains around cells from both the continuously and pulse-labeled populations could be fit at the 5% confidence level with a Poisson distribution modified to take into consideration the volume distribution of each population of cells analyzed. These findings suggested that large changes in the density of RNA and protein do not occur in most cells as they increase in size; however, there may be decreases in the rate of protein synthesis in some large and small cells. The decrease in the rate of protein synthesis appears consistent with the hypothesis that new sites of envelope growth must be introduced into cells that are close to the division event to restore rapid growth. 相似文献
7.
MUDr. B. Jakoubek CSc. 《Cell and tissue research》1976,166(1):125-133
Summary
14C-uridine or 14C-orotic acid was injected into the third or fourth brain ventricle of adult rats. The rate of incorporation of these precursors into the RNA of various brain regions was studied by autoradiography. 0.5 hour, 1 hour, 2 hours and 4 hours after the injection of labelled uridine and/or orotic acid into the third ventricle, a very uneven labelling of different brain regions was observed. The highest grain density was found over the ventricular walls and in the closely adjacent brain tissue; the intensity of labelling decreased sharply with distance from the ventricular lumen. 24 hours after intraventricular injection, a medio-lateral gradient of grain density was no longer observed. An intense labelling of leptomeninx (especially at the base of the brain) and of ependymal cells was observed at all time intervals investigated. At time intervals 0.5–2 hours the grain density of these structures surpassed by a considerable amount the grain density over neurons, glial cells or neuropile.Two hours after the injection of 14C-orotic acid into the fourth ventricle, the grains were mainly localised over leptomeningeal cells and vessels at the base of the brain and in the adjacent narrow strip of brain tissue. The rest of the brain was only very faintly labelled. 相似文献
8.
Granulocytes were isolated from whole blood of guinea pigs by counterflow centrifugation and labeled with [14C]diisopropylfluorophosphate ([14C]DFP). One-half of the labeled cells was injected intravenously via the femoral vein into a guinea pig, while the other half was cryogenically preserved with 5% dimethyl sulfoxide (DMSO), 6% hydroxyethyl starch (HES), and 4% human albumin, at a rate of 4 °C per minute by storage at ?80 °C and then stored for 3 days at ?80 °C. Ninety percent of the isolated granulocytes were recovered after cryogenic preservation, thawing, and washing. Aliquots before injection all produced fluorescein from fluorescein diacetate and excluded ethidium bromide. Latex ingestion was 78% and yeast ingestion was 75%. The frozen-thawed-washed-resuspended labeled granulocytes were injected into a second guinea pig. Paired animals sacrificed 35 min after injection were examined in whole-body sections for distribution of radiolabeled granulocytes to the tissues. In two pairs of animals, activity was found in the lung, liver, spleen, and kidney. The technique does not permit a distinction between fresh and cryopreserved granulocytes although there was a greater deposition of fresh cells in the liver and spleen. No activity was found in the blood of the vena cava in animals with either fresh or frozen cells. An animal injected with free [14C]DFP revealed a vascular distribution with high activity in blood, lung, and kidney, and less activity in the liver and spleen. The data indicate that radiolabeled, cryogenically preserved guinea pig granulocytes exhibited a tissue distribution qualitatively similar to fresh granulocytes, and free [14C]DFP infused without granulocytes differed qualitatively and quantitatively from fresh and cryopreserved granulocytes. 相似文献
9.
Transplantable reticulum cell sarcoma (RCS) cells were labeled with 3H-uridine or 3H-thymidine in vitro and injected intravenously into normal and irradiated syngeneic SJL/J mice. RCS cells exhibited typical B cell migration characteristics in peripheral lymphoid organs in both normal and irradiated recipients, localizing in follicles in a pattern resembling that of labeled normal bone marrow cells. However, over the first 72 hr after transfer, RCS cells diluted their label much less in irradiated than in normal recipients, reflecting their inability to proliferate in the irradiated hosts. The presence of unlabeled tumor cells did not significantly affect the distribution of labeled normal bone marrow or lymph node cells in the recipients. Thus, RCS fails to grow in irradiated recipients in spite of undisturbed homing characteristics and in the absence of any evidence of cytotoxic influences from the host. 相似文献
10.
Lucien G. Caro 《The Journal of cell biology》1961,9(3):539-553
If thin sections of Escherichia coli, labeled uniformly with tritium, are radioautographed calculations, based on the distribution of section sizes show that the number of H3 decays per section should be very close to a Poisson distribution. We might, therefore, expect that the distribution of radioautographic grain counts among random cross-sections should follow a Poisson distribution. It can then be inferred that a deviation from a Poisson indicates a high concentration of label in a preferred region. This region can then be identified by analysis of serial section and comparison with electron micrographs. Sections of cells labeled with leucine-H3 gave a Poisson distribution of grain counts, and it was concluded that proteins were distributed fairly uniformly throughout the cell. The situation was not changed if labeled cells were placed in chloramphenicol or if very short pulses of label were used. When Escherichia coli is grown in presence of chloramphenicol a major morphological change concerns the nuclear region: it becomes more regular in outline, nearly spherical, and occupies a smaller proportion of the cell length. The previously described association between DNA labeled with thymidine-H3 and the nuclear region was confirmed by showing that the distribution of the label in the cell followed exactly the morphological changes of the nuclear region. It was also shown that the concentration of DNA in the nuclear region was at least 45 times higher than that of the cytoplasm. Several morphological features of cells grown in chloramphenicol and examined in the electron microscope are discussed. 相似文献
11.
The experiments were carried out on human leucocytes cultured in vitro. We studied the distribution of silver grains over metaphase chromosomes after pulse-labeling of cells with H3-lysine in S- and G2 phases. It was found that the grain number per chromosome of the pairs No. 1–3, 13–15 is proportional to their lengths. The probability of incorporation of labeled proteins into each of the homologous chromosomes of the first pair is equal to 0.5 found from the results of statistical analysis of silver grain counts. In cells with karyotype XXX labeled in late-S, the grain number per chromosome in the subgroup 6-X-7 is uniform. In these cells there is no difference in labeling densities among chromosomes of the group A. The data obtained suggest that the formation of the protein component in autosomes of different groups as well as in homologous autosomes and sex-chromosomes proceeds simultaneously and at equal rate. 相似文献
12.
Autoradiographic tracing using tritium labeled compounds or cells is a common laboratory technique for light and electron microscopy. This report describes a chemo-graphic effect associated with certain cells in sections from tissues embedded in the new methacrylate plastic embedding compounds. When tissue sections from rats and rhesus monkeys that received no radioisotope were coated with nuclear track emulsion and subsequently developed, cells with morphologic characteristics of mast cells showed significant grain formation over the entire cell. Three different types of methacrylate plastics were tested using rat and monkey tissues and all three were found to promote grain formation over mast cells; however, this phenomenon was not seen in similar tissue sections from paraffin or epoxy embedded material. The properties of methacrylate plastics which promote positive chemography by mast cells may reflect the greater permeability of this class of plastics. Due to their wide tissue distribution, the presence of such chemographically active cells could cause false estimates of the distribution of either exogenous radiolabeled cells or radioisotopes within many tissues. 相似文献
13.
Autoradiographic tracing using tritium labeled compounds or cells is a common laboratory technique for light and electron microscopy. This report describes a chemographic effect associated with certain cells in sections from tissues embedded in the new methacrylate plastic embedding compounds. When tissue sections from rats and rhesus monkeys that received no radioisotope were coated with nuclear track emulsion and subsequently developed, cells with morphologic characteristics of mast cells showed significant grain formation over the entire cell. Three different types of methacrylate plastics were tested using rat and monkey tissues and all three were found to promote grain formation over mast cells; however, this phenomenon was not seen in similar tissue sections from paraffin or epoxy embedded material. The properties of methacrylate plastics which promote positive chemography by mast cells may reflect the greater permeability of this class of plastics. Due to their wide tissue distribution, the presence of such chemographically active cells could cause false estimates of the distribution of either exogenous radiolabeled cells or radioisotopes within many tissues. 相似文献
14.
Lars E. Ericson 《Cell and tissue research》1971,113(3):441-449
Summary The uptake and distribution of radioactivity in vascular adrenergic nerves in the mouse pancreas following the injection of tritiated 5-hydroxytryptophan was studied by means of electron microscopic autoradiography. Autoradiographic silver grains were found selectively accumulated over axonal profiles. Quantitative analysis revealed a characteristic intraneuronal distribution of the silver grains, most of which probably represent 5-hydroxytryptamine formed by decarboxylation from the labeled precursor. Thus, the grain density over adrenergic nerve terminals, containing a mixed population of vesicles and granules, was about 5 times higher than the grain density recorded over non-terminal axonal parts and at least 20 times higher than the grain density found over surrounding adventitial tissue and smooth muscle cells. This was interpreted as an evidence that 5-hydroxytryptamine was taken up and stored in adrenergic terminals. 相似文献
15.
A cut was made on the ear conch of mouse and an extract containing epidermal chalone was injected subcutaneously 2 days later. The time changes after the chalone administration in the number of cells labeled with 3H-thymidine, in the number of grains on labeled cells and in the number of mitoses within the regenerating epidermis surrounding the wound were investigated by means of autoradiography (ARG). Grain counts decreased temporarily in early phase (0–2 h) after chalone injection. This decrease in grain count resulted in a decrease in the number of labeled cells on the ARG of a short exposure but not in that on the ARG of a long exposure. A decrease in the number of labeled cells on the ARG of a long exposure was evident at 6 h when the grain counts reverted to a level similar to the control without chalone. The number of mitoses reached a minimum at 2 h and then recovered quickly, indicating a rapid disappearance of the inhibition of cells in G 2 from entering M phase. Mitoses decreased again thereafter, presumably as a result caused by inhibition of cells in the preceding S phase from completing DNA synthesis. The extract made similarly from liver or kidney affected neither the mitotic nor the DNA synthetic activities.These results indicate that the epidermal chalone or chalones inhibit the epidermal cell proliferation in, at least, 3 different processes of the cell cycle; the DNA synthesis in S phase, the transition from G 1 to S phase and the transition from G 2 to M phase. 相似文献
16.
Dr. Antonio Haddad Georges Pelletier Fausto Marchi Iara L. G. Brasileiro 《Cell and tissue research》1977,177(1):67-79
Summary Fucose-3H was injected into the cerebral ventricle of rats that were killed at several time intervals after injection. Semi-thin sections of the supraoptic nucleus and neurohypophysis were processed for radioautography and analysed quantitatively. Silver grains indicating the site of fucose-labeled glycoproteins were first located at the perinuclear region of the secretory neurons. The highest silver-grain density in these cells was observed at 2 h after injection, declining afterwards. Silver grains over the neurohypophysis were observed from 2 h on, reached a peak at 1 day after injection and decreased in the subsequent time intervals. The distributions of the silver grains over the neurohypophysis fitted Poissonian distributions and these were shown to be heterogeneous at the several time intervals. Pituicytes were not labeled. The percentage of silver grains over the Herring bodies increased with time. In rats deprived of water after fucose-3H injection there was a great increase in the release of labeled glycoproteins from the neurohypophysis. These results indicate that the glycoproteins synthesized by the secretory neurons of the hypothalamic nuclei are secreted in the neurohypophysis. 相似文献
17.
Summary Rat lymphokine-activated killer (LAK) cells, generated by adhering rat splenocytes isolated from the 52% Percoll density fraction to plastic flasks, demonstrate restricted in vivo tissue distribution, localizing in the lungs and liver after 2 h, but redistributing into the liver and spleen 24 h after i.v. administration. However, a different pattern of distribution was observed when this population of LAK cells was labeled with one of four commonly used radioisotopes. For example, LAK cells showed a high distribution into the lungs 30 min after administration when labeled with51Cr,125I-dUrd or111In-oxine, whereas111InCl-labeled LAK cells showed an equal distribution into the blood, lungs and liver at this time. Two hours after administration, cells labeled with111In-oxine showed an equivalent distribution into the lungs and liver, those labeled with125I-dUrd or51Cr showed a high accumulation in the lungs, whereas those labeled with111In-Cl entered more into the liver and blood. The pattern of distribution of111In-Cl- or111In-oxine-labeled cells was confirmed using gamma camera imaging analysis. By 24 h, LAK cells labeled with111InCl,111In-oxine or51Cr distributed in the liver and spleen in variable concentrations. In contrast, cells labeled with125I-dUrd were not detected in any organ tested.This study was paralleled by monitoring the distribution of LAK cells labeled with Hoechst 33342 (H33342) and analyzed for the presence of fluoresceinated cells in different organs either by flow cytometry analysis, or in frozen section. The data indicate that the distribution pattern of LAK cells labeled with111In-oxine is the closest to the distribution of H33342-labeled cells. Of all the radioisotopes used,125I-dUrd has the most disadvantages and is not recommended for monitoring the in vivo distribution of leukocytes. 相似文献
18.
P. Basse R. B. Herberman M. Hokland R. H. Goldfarb 《Cancer immunology, immunotherapy : CII》1992,34(4):221-227
Summary Assessment of the tissue distribution of adoptively transferred adherent lymphokine-activated killer A-LAK) cells by use of51Cr indicated that these effector cells, after an initial phase in the lungs, distributed in high numbers to liver and spleen (30% and 10% of injected dose, respectively). However, when this experiment was repeated with125IdUrd as cell label, fewer than 2% and 0.5% of the injected cells distributed into liver and spleen respectively. To analyse this discrepancy, we compared the tissue distribution of51Cr- and125IdUrd-labelled A-LAK cells with that indicated by alternative direct visual methods for identification of the injected cells, such as fluorescent dyes (rhodamine and H33342) or immunohistochemical staining of asialo-GM1-positive cells. The number of i. v. injected A-LAK cells found in the liver by all visual methods ranged from 1% to 5% of the injected dose, supporting the data obtained with125IdUrd, whereas 25%–30% of the51Cr label was consistently found in this organ. Autoradiography of the liver 24 h after i. v. injection of51Cr-labelled cells revealed a background activity that was four- to fivefold higher than the control level, indicating substantial non-specific accumulation in the liver of51Cr released from A-LAK cells. We conclude that51Cr cannot be reliably used in investigations of cell traffic to the liver because of non-specific accumulation of the51Cr label, particularly in this organ. In contrast, labelling with125IdUrd or rhodamine and immunohistochemical staining of asialo-GM1-positive cells appear to be reliable and essentially equivalent methods for investigations of the fate of adoptively transferred A-LAK cells. Using these methods, we found that only few A-LAK cells redistribute to the liver upon i. v., i. e. systemic, injection, whereas 40%–50% of locally (intraportally) injected A-LAK cells remain in the liver for at least 24 h. 相似文献
19.
CELL KINETICS OF IRRADIATED EXPERIMENTAL TUMORS: CELL TRANSITION FROM THE NON-PROLIFERATING TO THE PROLIFERATING POOL 总被引:1,自引:0,他引:1
Parenchymal tumor cells of murine mammary carcinomas can be divided into two pools, using nucleoli as morphological ‘markers’. Cells with dense nucleoli traverse the cell cycle and divide, thus constituting the proliferating pool. Cells with trabeculate or ring-shaped nucleoli either proceed slowly through G1 phase or are arrested in it. The role of these non-proliferating, G1 phase-confined cells in tumor regeneration was studied in vivo after a subcurative dose of X-irradiation in two transplantable tumor lines. Tumor-bearing mice were continuously injected with methyl[3H]thymidine before and after irradiation. Finally, the labeling was discontinued, mice injected with vincristine sulfate and cells arrested in metaphase were accumulated over a 10-hr period. Two clearly delineated groups of vincristinearrested mitoses emerged in autoradiograms prepared from tumor tissue at the time of starting tumor regrowth: one group with the silver-grain counts corresponding to the background level, the other with heavily labeled mitoses. As the only source of unlabeled mitoses was unlabeled G1 phase-confined cells persisting in the tumor, this observation indicated cell transition from the non-proliferating to the proliferating pool, which took place in the initial phase of the tumor regrowth. Unlabeled progenitors have apparently remained in G1 phase for at least 5–12 days after irradiation. 相似文献
20.
Suemizu H Hasegawa M Kawai K Taniguchi K Monnai M Wakui M Suematsu M Ito M Peltz G Nakamura M 《Biochemical and biophysical research communications》2008,377(1):248-252
Severely immunodeficient NOD/Shi-scid IL2Rgnull (NOG) mice are used as recipients for human tissue transplantation, which produces chimeric mice with various types of human tissue. NOG mice expressing transgenic urokinase-type plasminogen activator in the liver (uPA-NOG) were produced. Human hepatocytes injected into uPA-NOG mice repopulated the recipient livers with human cells. The uPA-NOG model has several advantages over previously produced chimeric mouse models of human liver: (1) the severely immunodeficient NOG background enables higher xenogeneic cell engraftment; (2) the absence of neonatal lethality enables mating of homozygotes, which increased the efficacy of homozygote production; and (3) donor xenogeneic human hepatocytes could be readily transplanted into young uPA-NOG mice, which provide easier surgical manipulation and improved recipient survival. 相似文献