PCR引物特异性核查系统(PSC)的构建与应用

聚合酶链式反应(PCR)虽已广泛用于分子生物学研究中,然而PCR实验中的非特异性产物问题将直接影响PCR的效率,在多重PCR实验中更是如此。为了最大限度地降低非特异性产物的出现率,同时避免用户频繁使用Blast比对检查非特异性,我们开发了基于NCBI-Blast的引物评估和模板DNA特异性结合能力评估的核查系统PSC(Primer Specificity Checking,http://biocompute.bmi.ac.cn/PSC),并基于虚拟PCR实验确定了用于引物质量核查计算的多种参数,能够在线提供多个物种的引物特异性核查结果。该系统可以有效地对引物序列可能产生的所有非特异性扩增进行预测,有助于实验前引物优化或者对非特异扩增结果进行解释,最终达到提高PCR效率的目的。     

THE 2ND CONGRESS OF THE PALEOBOTANICAL SOCIETY OF PSC HELD IN NANJING

The 2nd Congress of the Paleobotanical Society of Palaeontological Society and the Paleobolanical symposium (1987) held from November 24th to 27th at Nanjing, Jiangsu. Present at the meeting were 86 Chinese paleobotanists and several students from research institutes, universities, colleges, natural historical museums and geological, coal- and petroleum-prospecting parties.     

Inaugural Meeting of the Bivalvia Research Group of PSC and the 1st Bivalvia Symposium Held in Emei, Sichuan Province

The inaugural meeting of the National Bivalvia Re-search Group of PSC and the 1st Bivalvia symposiumwere held from Nov. 15 to 21. 1983 in Emei, Sichuan.75 participants, representing 53 organizations in cluding     

Severe liver disease resembling PSC in mice with K5-Cre mediated deletion of Krüppel-like factor 5 (Klf5)

Transgenic Research - Chronic cholestatic liver diseases including primary sclerosing cholangitis (PSC) present a complex spectrum with regards to the cause, age of manifestation and...