共查询到20条相似文献,搜索用时 37 毫秒
1.
We report on the isolation and characterization of eight microsatellite markers from repetitive DNA enriched libraries for
Cryptocarya chinensis from lower subtropical China. These are the first microsatellite loci reported for Cryptocarya species. The number of alleles ranged from three to nine. Observed and expected heterozygosities ranged from 0.0518 to 0.9910
and 0.5241 to 0.7935 for polymorphic loci, respectively. These markers will allow analyses of the baseline genetic variability
and population structure of C. chinensis to enrich our scientific understanding of forest fragmentation on genetic health of this species and provide strategies for
effective conservation and management in this area. 相似文献
2.
Cupressus chenggiana S. Y. Hu (Cupressaceae) is an endemic and endangered conifer species in southwest China. In order to study the population
genetics and design the effective conservation methods, we aimed to develop microsatellite primers for this species in the
present study. We developed eight new microsatellite loci for this species through biotin capture method. Polymorphism of
each locus was further assessed in 18 individuals from three geographically distant populations. The number of alleles per
locus ranged from 6 to 11 with an average of 8.13. The observed and expected heterozygosities ranged from 0.219 to 0.296 and
from 0.374 to 0.470, with averages of 0.254 and 0.417, respectively. We further found that three of nine microsatellite loci
developed previously for another congeneric species showed polymorphic banding patters. We performed primer-crossing tests
of these loci in the other two congeneric species which are closely related to C. chenggiana (C. gigantea and C. duclouxiana). These microsatellite markers would be effective for analyzing genetic diversity and population genetic structure of this
species and its morphological differentiation with the close relatives. 相似文献
3.
Genetiana crassicaulis is one of famous Chinese medicinal plant. The over-collection for its root has caused its dramatic reduction. In order to
devise adequate conservation and management strategies for this species, it is important to characterize its genetic diversity
and understand its population structure. Here, 10 polymorphic microsatellite markers have been developed. AC/TG microsatellite
was enriched by combining biotin capture method. Polymorphism of each locus was assessed in 30 individuals from six populations.
The number of alleles ranged from 2 to 9 and the expected heterozygosity ranged from 0.32 to 0.78. 相似文献
4.
Echinops setifer is an endangered grassland plant species in Japan. We isolated and characterized nine microsatellite loci in this species.
The number of alleles ranged from 3 to 7 and expected heterozygosities from 0.108 to 0.761. These markers described here will
be useful for investigating the genetic diversity, genetic structure and gene flow, and planning for conservation of E. setifer. 相似文献
5.
Metrosideros boninensis is an endangered endemic plant species from the Bonin Islands. We isolated and characterized nine microsatellite loci in
this species. The expected heterozygosities of these nine markers ranged from 0.127 to 0.768. These markers described here
will be useful for investigating the genetic diversity, genetic structure and gene flow, and planning for conservation of
M. boninensis. 相似文献
6.
Inmaculada López-Flores Víctor N. Suárez-Santiago Ana T. Romero-García Roberto de la Herrán Gabriel Blanca 《Conservation Genetics》2008,9(6):1695-1697
We report on the isolation and characterization of eight microsatellite markers from enriched libraries for Arenaria nevadensis, one of the most critically endangered plant species in the Iberian Peninsula. These are the first microsatellite loci reported
for Arenaria species. The number of alleles ranged from two to eight, and the expected heterozygosity from 0.067 to 0.873. These markers
will be useful for characterizing the genetic diversity in A. nevadensis and understanding its population structure, and will provide important genetic data for the conservation and recovery of
this species. 相似文献
7.
Dahai Gao Qian Wang Yuxia Wu Haiyan Xu Qiushi Yu Jianquan Liu 《Conservation Genetics》2008,9(4):953-955
Thellungiella
salsuginea (Brassiaceae) is a typical halophyte which can tolerate extreme cold, drought, and salinity. In order to understand the adaptive
evolution of this species in the arid habitats, it is important to know its genetic structure. In this study, 17 polymorphic
microsatellite loci were isolated and characterized from an enrichment genomic library of this species. We further assessed
the polymorphisms of each locus in 18 individuals from nine geographically distant populations. The number of alleles per
locus ranged from six to fourteen. The observed and expected heterozygosity ranged from 0.17 to 0.28 and 0.32 to 0.45, respectively.
These markers have been crossly checked in another congeneric species, T. halophila. These microsatellite markers will be useful for investigating population genetics and adaptive evolution of this species
and morphological divergence between and it and the closely related species. 相似文献
8.
Scapharca broughtonii is an over-exploited bivalve in China. In order to promote a conservation program for this species, it is necessary to evaluate
its genetic diversity and population genetics. For this purpose, we isolated twelve novel polymorphic microsatellite markers
and examined their allelic variation using 40 wild individuals collected from one locality along the Chinese coast. The number
of alleles ranged from 2 to 22 per locus with the expected heterozygosities ranging from 0.444 to 0.944. These markers will
be useful for the assessment of genetic variation for this species. 相似文献
9.
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, the extent of genetic variation in the captive alligators
of the Changxing Reserve Center was investigated using microsatellite markers derived from American alligators. Out of 22
loci employed, 21 were successfully amplified in the Chinese alligator. Sequence analysis showed loci in American alligators
had a bigger average size than that of the Chinese alligators and the longest allele of an individual locus almost always
existed in the species with longer stretch of repeat units. Eight of the 22 loci were found to be polymorphic with a total
of 26 alleles present among 32 animals scored, yielding an average of 3.25 alleles per polymorphic locus. The expected heterozygosity
(H
E) ranged at a moderate level from 0.4385 to 0.7163 in this population. Compared to that in the American alligators, a lower
level of microsatellite diversity existed in the Changxing population as revealed by about 46% fewer alleles per locus and
smaller H
E at the homologous loci. The average exclusion power and the ability to detect shared genotypes and multiple paternity were
evaluated for those markers. Results suggested that when the polymorphic loci were combined, they could be sensitive markers
in genetic diversity study and relatedness inference within the Chinese alligator populations. The level of genetic diversity
present in the current Changxing population indicated an important resource to complement reintroductions based on the individuals
from the other population. In addition, the microsatellite markers and their associated diversity characterized in this population
could be utilized to further investigate the genetic status of this species. 相似文献
10.
The first set of nine microsatellite markers for the endangered sea urchin Strongylocentrotus nudus was developed from EST databases of S. purpuratus. Number of alleles per locus ranged from two to thirteen. The observed and expected heterozygosities ranged from 0.000 to
0.645 and from 0.063 to 0.912, respectively. These informative microsatellite markers will be useful in studies of population
genetic structure for this species. 相似文献
11.
Qian Zhang Yan-Zhuo Yang Gui-Li Wu Dong-Yuan Zhang Jian-Quan Liu 《Conservation Genetics》2008,9(3):767-769
Juniperus przewalskii (Cupressaceae) is a dominant tree species endemic to the northeast Qinghai-Tibetan Plateau. This species plays an important
role in maintaining the arid ecosystem in this region. However, natural distributions of this species have been declined.
In order to develop effective conservation methods, it is important to know the distribution of the genetic diversity within
and among populations. In this study, we developed nine new microsatellite loci for this species. We used the combining biotin
capture method to enrich AG/CT/AC/GGT microsatellites. The polymorphisms of each locus were further assessed in 12 individuals
from four geographically distant populations. The number of alleles per locus varied from three to six and expected heterozygosity
ranged from 0.58 to 0.70. These loci together provide a useful tool to investigate the genetic diversity of this species.
In addition, all markers have been crossly checked in the other four congeneric species. 相似文献
12.
Ostryopsis (Betulaceae) is a samll genus endemic to China with only two species. Both of them play an important role in restoring the
local ecosystems. The distribution of genetic diversity between and within populations in each species are important to further
utilize the wild genetic resources and explore the interspecific divergence. In this study, we developed 10 microsatellite
loci from O. davidiana by the combining biotin capture method for the first time. A total of 27 microsatellite sequences were recovered through
screening the library and 10 of them are polymorphic. The number of alleles per locus in 18 sampled individuals ranged from
3 to 6, expected heterozygosity and observed heterozygosity ranged from 0.2958 to 0.4767 and from 0.1591 to 0.2997, respectively.
In addition, all markers have been crossly checked in the other congeneric species. These microsatellite markers would together
provide a useful tool for investigating the genetic diversity and structure of both species and speciation mechanism between
them. 相似文献
13.
Calligonum mongolicum Turcz. (Polygonaceae) is a typical drought desert plant which can tolerate extreme drought, cold, and other stress in the
northern extreme drought desert of China. They are playing an important role in maintaining the local ecosystems in these
regions. In order to understand the adaptive evolution and develop effective conservation methods of this species in the arid
habitats, it is important to know its genetic structure. In this study, 14 polymorphic microsatellite loci were isolated and
characterized from an enrichment genomic library of this species. We further assessed the polymorphisms of each locus in 18
individuals from six geographically distant populations. The number of alleles per locus ranged from three to six. The observed
and expected heterozygosity ranged from 0.24 to 0.35 and 0.38 to 0.57, respectively. These markers have been crossed checked
in another congeneric species, C. mongolicum. These microsatellite markers will be useful for investigating the genetic structure and devising conservation strategies
of this species, and morphological divergence between and it and the closely related species. 相似文献
14.
Ramesh K. Aggarwal Albert Lalremruata Thirumalaisamy P. Velavan Ayyadevara Pavani Sowjanya Lalji Singh 《Conservation Genetics》2008,9(4):981-984
Olive ridleys, one of the widely distributed marine turtle species has undergone declines in recent years due to multiple
anthropogenic factors warranting conservation efforts for which assessment of genetic variability in existing populations
become critical. Here we describe development of ten new microsatellite markers from a short sequence repeat-enriched partial
genomic DNA library, which are found to be highly informative for genetic studies. Eight of these markers when tested on 83
olive ridley turtles revealed high allelic diversity (4–27 alleles per marker), and high observed and expected heterozygosity
estimates that ranged from 0.29 to 0.82 and 0.62 to 0.94, respectively. Two microsatellites were monomorphic in the tested
olive ridley samples, but were found to be informative/polymorphic when tested on related marine turtle species. More importantly,
nine of the new markers showed robust cross-species amplifications in three related species Dermochelys coriacea, Chelonia mydas and Eretmochelys imbricata. Thus, this study describes ten new microsatellite markers and also demonstrates their potential as efficient genetic markers
in studies related to parentage analysis, population structure, phylogeography and species relationships of olive ridleys
and other marine turtle species. 相似文献
15.
Eleven polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Akebia trifoliate ssp. australis. The number of allele per locus ranged from 3 to 14. The observed heterozygosity and expected heterozygosity at population
level were 0.196–1.000 and 0.522–0.902, respectively. In addition, this set of microsatellites produced robust cross-species
amplification in other two related taxa, suggesting these microsatellite markers should provide a useful tool for genetic
and conservation studies of the Akebia species. 相似文献
16.
Codonopsis pilosula Nannf., as an important traditional Chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite.
In this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture
method. The polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations.
The number of alleles per locus ranged from 6 to 11. The observed and expected heterozygosity ranged from 0.15 to 0.24 and
0.27 to 0.40, respectively. We further performed cross-priming tests of these loci in the other three congeneric species.
These microsatellite markers will be useful for investigating genetic diversity within and between populations of these species. 相似文献
17.
Hung-Du Lin Teh-Wang Lee Fang-Jiao Lin Chung-Jian Lin Tzen-Yuh Chiang 《Conservation Genetics》2008,9(4):945-947
Pararasbora moltrechti (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the central Taiwan. In order to undertake
a conservation program for this species in Taiwan, it is essential to evaluate its genetic diversity and population structure.
For this purpose, we described the development of eight microsatellite loci in P. moltrechti for genetic studies. These new molecular markers were tested in 36 individuals of the rare species. The number of alleles
per locus ranged from 8 to 14. As the expected heterozygosity varied from 0.770 to 0.932, all microsatellite loci, however,
were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency.
Hung-Du Lin and Teh-Wang Lee contributed equally. 相似文献
18.
Lycopodium fordii Bak. (Lycopodiaceae, Pteridophyta) is a gardening plant with a native, fragmentary distribution in Taiwan. In this study,
we described the development of eleven microsatellite loci in L. fordii for genetic studies. These new markers were tested in 16 individuals of three populations. The number of alleles ranged from
1 to 5 and the expected heterozygosity from 0.41746 to 0.72222. Four of the nine polymorphic loci were significantly deviated
from Hardy-Weinberg expectations due to the heterozygote deficiency. The microsatellite markers have also been proved as informative
genetic markers for other 15 Lycopodium species. 相似文献
19.
The rummy‐nose tetra, Hemigrammus bleheri, is a small fish found in forest streams of the Rio Negro floodplain, central Amazonia. This species is popular in aquaria and used as an ornamental fishery resource by the riverine people from middle Rio Negro. Here we describe eight microsatellite DNA loci for rummy‐nose tetras. Number of alleles and heterozygosity per locus in a sample of 30 fish ranged from five to 22 and from 0.52 to 0.96, respectively. These highly variable genetic markers provide important tools for investigating population history and identifying conservation units in rummy‐nose tetras. 相似文献
20.
Stuart W. A’Hara Joan Elizabeth Cottrell 《Molecular breeding : new strategies in plant improvement》2009,23(2):349-355
Robust, polymorphic microsatellite DNA markers (simple sequence repeats—SSRs) are valuable tools for a range of tree conservation
and breeding applications. SSRs are routinely used in the study of population genetic structure and diversity, pedigree reconstruction
and genetic linkage mapping. Their abundance in the genome, co-dominant inheritance and potential for cross-species amplification
make microsatellites highly prized markers. This paper characterises 22 novel genomic polymorphic microsatellite loci for
Sitka spruce (Picea sitchensis (Bong.) Carr.). Amplification of DNA from Sitka spruce material was carried out both with a set of unrelated trees to obtain
diversity statistics for each locus, and with the progeny of a full-sib family to test simple Mendelian inheritance. Observed
heterozygosity ranged from 0.38 to 0.91 and allele number per locus ranged from 6 to 21, with a mean of 12.2. In addition,
the primer pairs were tested with DNA from Norway spruce (P. abies) and white spruce (P. glauca) to investigate their potential for cross-species amplification and ten loci amplified in all three species. The results
from these genomic microsatellites are compared to data generated from microsatellites derived from Picea EST libraries. In summary, this novel, highly polymorphic markers represent a significant addition to the rapidly expanding
Picea genomics tool-box.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献