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1.
Three new unusual compound chromosomes have been synthesized in Drosophila melanogaster. They consist of two homologous autosomes joined together in the new order: right arm, left arm, centromere, left arm, right arm, for each of the two major autosomes, and one in which chromosomes 2 and 3 have been combined in the order: right arm of 2, left arm of 2, centromere, left arm of 3, right arm of 3. The attachments of the autosomal arms were accomplished by obtaining chromosome breaks at or very close to the ends of the left arms of the autosomes such that no essential chromosome material has been removed; the compounds derived from them are therefore referred to as entire compounds. These large chromosomes are recovered in progeny with frequencies lower than expectation partly because of zygote mortality associated with these chromosomes, and partly because of a failure of spermiogenesis. 相似文献
2.
Meiotic Behavior of Compound Autosomes in Females of DROSOPHILA MELANOGASTER: Interchromosomal Effects and the Source of Spontaneous Nonsegregation
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In females of Drosophila melanogaster, compound autosomes enter the repulsion phase of meiosis uncommitted to a particular segregation pattern because their centromeres are not restricted to a bivalent pairing complex as a consequence of crossing over. Their distribution at anaphase, therefore, is determined by some meiotic property other than exchange pairing, a property that for many years has been associated with the concept of nonhomologous pairing. In the absence of heterologous rearrangements or a free Y chromosome, C(3L) and C(3R) are usually recovered in separate gametes, that is as products of meiotic segregation. Nevertheless, there is a regular, albeit infrequent, recovery of reciprocal meiotic products (the nonsegregational products) that are disomic and nullosomic for compound thirds. The frequency of these exceptions, which is normally between 0.5 and 5.0%, differs for the various strains examined, but remains constant for any given strain. Since previous studies have not uncovered a cause for this base level of nonsegregation, it has been referred to as the spontaneous frequency. In this study, crosses between males and females whose X chromosomes, as well as compound autosomes, are differentially marked reveal a highly significant positive correlation between the frequency of compound-autosome nonsegregation and the frequency of X-chromosome nondisjunction. However, an inverse correlation is found when the frequency of nondisjunction is related to the frequency of crossing over in the proximal region of the X chromosome. These findings have been examined with reference to the distributive pairing and the chromocentral models and interpreted as demonstrating (1) that nonsegregational meiotic events arise primarily as a result of nonhomologous interactions, (2) that forces responsible for the segregation of nonhomologous chromosomes are properties of the chromocentral region, and (3) that these forces come into expression after the exchange processes are complete. 相似文献
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Meiotic Chromosome Behavior Influenced by Mutation-Altered Disjunction in DROSOPHILA MELANOGASTER Females 总被引:2,自引:1,他引:1
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Joseph O''Tousa 《Genetics》1982,102(3):503-524
The effects of a female-specific meiotic mutation, altered disjunction (ald: 361), are described. Although ald females show normal levels of meiotic exchange, sex- and 4th-chromosome nondisjunction occurs at an elevated level. A large proportion of the nondisjunction events is the result of nonhomologous disjunction of the sex and 4th chromosomes. These nonhomologous disjunction events, and probably all nondisjunction events occurring in ald females, are the result of two anomalies in chromosome behavior: (1) X chromosomes derived from exchange tetrads undergo nonhomologous disjunction and (2) the 4th chromosomes nonhomologously disjoin from larger chromosomes. There is at best a marginal effect of ald on the meiotic behavior of chromosomes 2 or 3. The results suggest that the ald+ gene product acts to prevent the participation of exchange X chromosomes and all 4th chromosomes in nonhomologous disjunction events. The possible role of ald+ in current models of the disjunction process is considered. 相似文献
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A genetic method for insect control was evaluated using the test organism, Drosophila melanogaster. The technique involved the displacement under a system of continuous reproduction, of standard strains by those carrying compound autosomes. The eradication of the replacements could subsequently be achieved through the use of temperature-sensitive lethal mutations.-While certain compound autosome strains failed to displace standards in population cages, even at the initial release ratio of 25:1, others were highly successful. Indeed, for some strains when the ratio of compounds to standards was as low as 9:1, the population rapidly went to fixation in favor of the compound line.-Hatchability was found to be an insufficient index of fitness to estimate the initial ratios of compounds to standards that would guarantee fixation of the former. Differences in other fitness components, such as development time, were detected that could seriously modify displacement, especially with continuous overlapping generations. The importance of examining the fitness of various compound lines and selecting the most competitive in cages, prior to field tests, cannot be overemphasized. 相似文献
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The Meiotic Origin of Temperature-Induced Crossovers in DROSOPHILA MELANOGASTER Females 总被引:3,自引:2,他引:1
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Grell RF 《Genetics》1966,54(2):411-421
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McKee B 《Genetics》1984,106(3):403-422
In Drosophila melanogaster males, deficiency for X heterochromatin causes high X-Y nondisjunction and skewed sex chromosome segregation ratios (meiotic drive). Y and XY classes are recovered poorly because of sperm dysfunction. In this study it was found that X heterochromatic deficiencies disrupt recovery not only of the Y chromosome but also of the X and autosomes, that both heterochromatic and euchromatic regions of chromosomes are affected and that the "sensitivity" of a chromosome to meiotic drive is a function of its length. Two models to explain these results are considered. One is a competitive model that proposes that all chromosomes must compete for a scarce chromosome-binding material in Xh(-) males. The failure to observe competitive interactions among chromosome recovery probabilities rules out this model. The second is a pairing model which holds that normal spermiogenesis requires X-Y pairing at special heterochromatic pairing sites. Unsaturated pairing sites become gametic lethals. This model fails to account for autosomal sensitivity to meiotic drive. It is also contradicted by evidence that saturation of Y-pairing sites fails to suppress meiotic drive in Xh(- ) males and that extra X-pairing sites in an otherwise normal male do not induce drive. It is argued that meiotic drive results from separation of X euchromatin from X heterochromatin. 相似文献
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The behaviour of two "meiotic drive" systems, Segregation-Distorter (SD) and the sex chromosome sc(4)sc(8) has been examined in the same meiocyte. It has been found that the two systems interact in a specific way. When the distorting effects of SD and sc(4)sc(8) are against each other, there is no detectable interaction. Each system is apparently oblivious to the presence of the other, gametes being produced according to independence expectations. However when the affected chromosomes are at the same meiotic pole an interaction occurs; the survival probability of the gamete containing both distorted chromosomal products is increased, rather than being decreased by the combined action of two systems. 相似文献
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A Meiotic Mutant Affecting Recombination in Female DROSOPHILA MELANOGASTER 总被引:4,自引:4,他引:0
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Dilys M. Parry 《Genetics》1973,73(3):465-486
mei-S282 is a female meiotic mutant isolated from a natural population of Drosophila melanogaster. It is a recessive mutation located at approximately map position 5 on the third chromosome which has two major effects. It causes a nonuniform decrease in recombination which is most drastic in distal chromosome regions and nondisjunction of all chromosome pairs is elevated at the first meiotic division. Nondisjunctional events are positively correlated; furthermore, nondisjoining chromosomes, themselves nonrecombinant, are preferentially recovered from cells in which nonhomologs are preferentially recovered from cells in which nonhomologs are also non-recombinant.-It is concluded that mei-S282 is a defect which occurs early in meiosis I prior to the time of exchange. In the mutant, the frequency of no-exchange tetrads for each of the major chromosomes is increased-and in cells which contain two or more no-exchange tetrads, an interaction between these chromosomes leads to correlated nondisjunction. mei-S282(+) then, is an exchange precondition necessary for the normal frequency and distribution of exchanges. 相似文献
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Genetic Analysis of Sex Chromosomal Meiotic Mutants in DROSOPHILA MELANOGASTER 总被引:2,自引:19,他引:2
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A total of 209 ethyl methanesulfonate-treated X chromosomes were screened for meiotic mutants that either (1) increased sex or fourth chromosome nondisjunction at either meiotic division in males; (2) allowed recombination in such males; (3) increased nondisjunction of the X chromosome at either meiotic division in females; or (4) caused such females, when mated to males heterozygous for Segregation-Distorter (SD) and a sensitive homolog to alter the strength of meiotic drive in males.-Twenty male-specific meiotic mutants were found. Though the rates of nondisjunction differed, all twenty mutants were qualitatively similar in that (1) they alter the disjunction of the X chromosome from the Y chromosome; (2) among the recovered sex-chromosome exceptional progeny, there is a large excess of those derived from nullo-XY as compared to XY gametes; (3) there is a negative correlation between the frequency of sex-chromosome exceptional progeny and the frequency of males among the regular progeny. In their effects on meiosis these mutants are similar to In(1)sc(4L)sc(8R), which is deleted for the basal heterochromatin. These mutants, however, have normal phenotypes and viabilities when examined as X/0 males, and furthermore, a mapping of two of the mutants places them in the euchromatin of the X chromosome. It is suggested that these mutants are in genes whose products are involved in insuring the proper functioning of the basal pairing sites which are deleted in In(1)sc(4L)sc(8R), and in addition that there is a close connection, perhaps causal, between the disruption of normal X-Y pairing (and, therefore, disjunction) and the occurrence of meiotic drive in the male.-Eleven mutants were found which increased nondisjunction in females. These mutants were characterized as to (1) the division at which they acted; (2) their effect on recombination; (3) their dominance; (4) their effects on disjunction of all four chromosome pairs. Five female mutants caused a nonuniform decrease in recombination, being most pronounced in distal regions, and an increase in first division nondisjunction of all chromosome pairs. Their behavior is consistent with the hypothesis that these mutants are defective in a process which is a precondition for exchange. Two female mutants were allelic and caused a uniform reduction in recombination for all intervals (though to different extents for the two alleles) and an increase in first-division nondisjunction of all chromosomes. Limited recombination data suggest that these mutants do not alter coincidence, and thus, following the arguments of Sandler et al. (1968), are defective in exchange rather than a precondiiton for exchange. A single female mutant behaves in a manner that is consistent with it being a defect in a gene whose functioning is essential for distributive pairing. Three of the female meiotic mutants cause abnormal chromosome behavior at a number of times in meiosis. Thus, nondisjunction at both meiotic divisions is increased, recombinant chromosomes nondisjoin, and there is a polarized alteration in recombination.-The striking differences between the types of control of meiosis in the two sexes is discussed and attention is drawn to the possible similarities between (1) the disjunction functions of exchange and the process specified by the chromosome-specific male mutants; and (2) the prevention of functional aneuploid gamete formation by distributive disjunction and meiotic drive. 相似文献
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A Meiotic Mutant Defective in Distributive Disjunction in DROSOPHILA MELANOGASTER 总被引:19,自引:11,他引:8
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Adelaide T. C. Carpenter 《Genetics》1973,73(3):393-428
The female meiotic mutant no distributive disjunction (symbol: nod) reduces the probability that a nonexchange chromosome will disjoin from either a nonexchange homolog or a nonhomolog; the mutant does not affect exchange or the disjunction of bivalents that have undergone exchange. Disjunction of nonexchange homologs was examined for all chromosome pairs; nonhomologous disjunction of the X chromosomes from the Y chromosome in XXY females, of compound chromosomes in females bearing attached-third chromosomes with and without a Y chromosome, and of the second chromosomes from the third chromosomes were also examined. The results suggest that the defect in nod is in the distributive pairing process. The frequencies and patterns of disjunction from a trivalent in nod females suggest that the distributive pairing process involves three separate events-pairing, orientation, and disjunction. The mutant nod appears to affect disjunction only. 相似文献
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Genetic Analysis of the Proximal Region of Chromosome 2 of DROSOPHILA MELANOGASTER. I. Detachment Products of Compound Autosomes 总被引:8,自引:6,他引:2
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To examine the genetic composition of proximal heterochromain in chromosome 2, the detachment of compound second autosomes, for generating proximal deficiencies, appeared a promising method. Compound seconds were detached by gamma radiation. A fraction of the detachment products were recessive lethals owing to proximal deficiencies. Analysis by inter se complementation, pseudo-dominance tests with proximal mutations and allelism tests with known deficiencies provided evidence for at least two loci between the centromere and the light locus in 2L and one locus in 2R between the rolled locus and the centromere. The data further demonstrate that rolled, and probably light, are located within the proximal heterochromatin. Thus, functional genetic loci are found in heterochromatin, albeit at low density. 相似文献
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Temperature-Sensitive Mutations in DROSOPHILA MELANOGASTER. IX. Dominant Cold-Sensitive Lethals on the Autosomes
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Ethyl methanesulfonate-treated autosomes were screened for the presence of dominant cold-sensitive (DCS) lethal mutations in Drosophila melanogaster. None was found among 6,552 treated and 168 untreated third chromosomes. Twenty-three DCS-L chromosomes which caused death at 17 degrees C but survived at 22 degrees C and 29 degrees C were recovered from 5,046 mutagenized chromosome 2's.-The DCS-L mutations all mapped around dp and appeared to be functionally allelic. Lethality of heterozygotes for most of the DCS-L's occurred over a prolonged interval from the embryonic through the larval instars. Prolonged incubation at 17 degrees C did not demonstrate any maternal effect on zygotic survival. 相似文献
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The Effect of Recombination-Defective Meiotic Mutants on Fourth-Chromosome Crossing over in DROSOPHILA MELANOGASTER
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Crossing over was measured on the normally achiasmate fourth chromosome in females homozygous for one of our different recombination-defective meiotic mutants. Under the influence of those meiotic mutants that affect the major chromosomes by altering the spatial distribution of exchanges, meiotic fourth-chromosome recombinants were recovered irrespective of whether or not the meiotic mutant decreases crossing over on the other chromosomes. No crossing over, on the other hand, was detected on chromosome 4 in either wild type or in the presence of a meiotic mutant that decreases the frequency, but does not affect the spatial distribution, of exchange on the major chromosomes. It is concluded from these observations that (a) in wild type there are regional constraints on exchange that can be attenuated or eliminated by the defects caused by recombination-defective meiotic mutants; [b] these very constraints account for the absence of recombination on chromosome 4 in wild type; and [c] despite being normally achiasmate, chromosome 4 responds to recombination-defective meiotic mutants in the same way as do the other chromosomes. 相似文献
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The Meiotic Effects of a Deficiency in DROSOPHILA MELANOGASTER: Identification of Two Dosage-Sensitive Sites
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Robbins LG 《Genetics》1980,94(2):361-381
Heterozygosity for a deficiency for the entire zeste-white region of the X chromosome of Drosophila melanogaster females causes both reduced recombination and increased nondisjunction. The location of the dosage-sensitive sites responsible for these two meiotic defects has been studied by use of a set of deficiencies that subdivide the region. Recombination is reduced when the zw7-zw11 region is present in one dose, while nondisjunction is increased only if the doses of both the zw8-zw10 and zw6-zw11 segments are reduced. Examination of trans heterozygotes of two deficiencies explicitly demonstrates the compound nature of the meiotic dose effect and further delimits the location of the proximal disjunctional site to the zw12-zw11 interval. In inversion/deficiency heterozygotes, reduced dose of the zw8-zw10 region alone, without reduced dose of the proximal site, yields increased nondisjunction, suggesting that the proximal element that affects disjunction is the same as that which affects recombination. Thus, the zeste-white region contains at least two dosagesensitive loci that affect meiosis: reduced dosage of one locus, in the zw7-zw11 interval, causes reduced recombination; reduced dose of another, in the zw8-zw10 region, increases the probability that nonexchange chromosomes will nondisjoin. A slight effect on the regional distribution of exchange may also be a property of the zw8-zw10 region locus, but could be an effect of yet another locus or of structural heterozygosity. The implications of these results for understanding meiotic control and the prospects for further analysis of the structure of the zeste-white interval are considered. 相似文献
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The effects of eight recombination-defective meiotic mutants on crossing over within the X heterochromatin were examined. Since none permit substantial frequencies of exchange within heterochromatin although six lessen or abolish constraints on the location of exchanges within euchromatin, the systems that prohibit exchange within heterochromatin and that govern where exchanges will occur in euchromatin are under separate genetic control.—A minor component of the effects of mei-218 is the production of nonhomologous exchanges; of mei-9 is the recovery of deleted chromatids; and of mei-41 is the recovery of deleted chromatids and/or a low frequency of heterochromatic exchanges. 相似文献
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