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1.
Borisjuk L Rolletschek H Radchuk R Weschke W Wobus U Weber H 《Plant biology (Stuttgart, Germany)》2004,6(4):375-386
2.
A cotyledon regulatory region is responsible for the different spatial expression patterns of Arabidopsis 2S albumin genes 总被引:5,自引:1,他引:4
Alexandre da Silva Conceição Enno Krebbers 《The Plant journal : for cell and molecular biology》1994,5(4):493-505
The 2S albumin genes of Arabidopsis thaliana are a model system to study gene expression during late embryogenesis. The at2S1 gene has previously been shown to be expressed essentially in the embryo axis, unlike at2S2, which is expressed throughout the embryo. Hybrid promoter constructs between at2S1 and at2S2 were introduced into Arabidopsis and used to identify a cotyledon regulatory region necessary for 2S albumin expression in palisade parenchyma and specific epidermal cells. Other promoter sequences flanking this tissue-specific promoter element were shown to control mRNA expression levels independently of the mRNA distribution throughout the embryos. Certain hybrid promoters resulted in the alteration of the time course of expression in cotyledons. Differential expression of 2S albumin genes is discussed in terms of layered cellular organization and mitotic activity throughout the embryo. 相似文献
3.
In this study, the distribution of water in pea seeds after harvesting at different seed stages was traced by magnetic resonance imaging (MRI). MRI visualized the process of water loss in maturing pea seeds. MR images showed local inhomogeneities of water distribution inside seeds. The intensity of the signal coming from water declined from the inner to the outer part of cotyledon tissue. This spatial inhomogeneity of water signals inside cotyledons may be correlated with the gradient of storage substances accumulation within cotyledons. Tissue localization of dehydrins showed the presence of dehydrin protein in the area of protovascular tissue of both the embryo axis and cotyledons. The temporal accumulation of two dehydrin proteins with molecular masses of 30 and 35kDa correlated well with seed desiccation. The pattern of dehydrin localization reflected the pattern of water distribution in the protovascular bundles region of maturing pea embryos, suggesting the involvement of these proteins in promoting water influx into the vascular bundles. 相似文献
4.
Repressing the expression of the SUCROSE NONFERMENTING-1-RELATED PROTEIN KINASE gene in pea embryo causes pleiotropic defects of maturation similar to an abscisic acid-insensitive phenotype 总被引:1,自引:0,他引:1
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The classic role of SUCROSE NONFERMENTING-1 (Snf1)-like kinases in eukaryotes is to adapt metabolism to environmental conditions such as nutrition, energy, and stress. During pea (Pisum sativum) seed maturation, developmental programs of growing embryos are adjusted to changing physiological and metabolic conditions. To understand regulation of the switch from cell proliferation to differentiation, SUCROSE NONFERMENTING-1-RELATED PROTEIN KINASE (SnRK1) was antisense repressed in pea seeds. Transgenic seeds show maturation defects, reduced conversion of sucrose into storage products, lower globulin content, frequently altered cotyledon surface, shape, and symmetry, as well as occasional precocious germination. Gene expression analysis of embryos using macroarrays of 5,548 seed-specific genes revealed 183 differentially expressed genes in two clusters, either delayed down-regulated or delayed up-regulated during transition. Delayed down-regulated genes are related to mitotic activity, gibberellic acid/brassinosteroid synthesis, stress response, and Ca2+ signal transduction. This specifies a developmentally younger status and conditional stress. Higher gene expression related to respiration/gluconeogenesis/fermentation is consistent with a role of SnRK1 in repressing energy-consuming processes in maturing cotyledons under low oxygen/energy availability. Delayed up-regulated genes are mainly related to storage protein synthesis and stress tolerance. Most of the phenotype resembles abscisic acid (ABA) insensitivity and may be explained by reduced Abi-3 expression. This may cause a reduction in ABA functions and/or a disconnection between metabolic and ABA signals, suggesting that SnRK1 is a mediator of ABA functions during pea seed maturation. SnRK1 repression also impairs gene expression associated with differentiation, independent from ABA functions, like regulation and signaling of developmental events, chromatin reorganization, cell wall synthesis, biosynthetic activity of plastids, and regulated proteolysis. 相似文献
5.
Dafeng Zhou Daqi Qian Carole L. Cramer Zhenbiao Yang 《The Plant journal : for cell and molecular biology》1997,12(4):921-930
Farnesylation mediates membrane targeting and in vivo activities of several key regulatory proteins such as Ras and Ras-related GTPases and protein kinases in yeast and mammals, and is implicated in cell cycle control and abscisic acid (ABA) signaling in plants. In this study, the developmental expression of a pea protein farnesyl-transferase (FTase) gene was examined using transgenic expression of the β-glucuronidase (GUS) gene fused to a 3.2 kb 5′ upstream sequence of the gene encoding the pea FTase β subunit. Coordinate expression of the GUS transgene and endogenous tobacco FTase β subunit gene in tobacco cell lines suggests that the 3.2 kb region contains the key FTase promoter elements. In transgenic tobacco plants, GUS expression is most prominent in meristematic tissues such as root tips, lateral root primordia and the shoot apex, supporting a role for FTase in the control of the cell cycle in plants. GUS activity was also detected in mature embryos and imbibed embryos, in accordance with a role for FTase in ABA signaling that modulates seed dormancy and germination. In addition, GUS activity was detected in regions that border two organs, e.g. junctions between stems and leaf petioles, cotyledons and hypocotyls, roots and hypocotyls, and primary and secondary roots. GUS is expressed in phloem complexes that are adjacent to actively growing tissues such as young leaves, roots of light-grown seedlings, and hypocotyls of dark-grown seedlings. Both light and sugar (e.g. sucrose) treatments repressed GUS expression in dark-grown seedlings. These expression patterns suggest a potential involvement of FTase in the regulation of nutrient allocation into actively growing tissues. 相似文献
6.
Dehaye Laure Duval Manuel Viguier Delphine Yaxley Jennifer Job Dominique 《Plant molecular biology》1997,35(5):605-621
Besides biotin-dependent carboxylases, which play key roles in basic metabolism, SBP65 (seed biotinylated protein of 65 kDa of apparent molecular mass), an atypical biotinylated protein, has been described in pea plants. This seed-specific protein is devoid of any carboxylase activity, and shares many physiological and molecular features with late embryogenesis-abundant (Lea) proteins. In a first step toward understanding the role of this peculiar protein, we have demonstrated the role of abscisic acid (ABA) and of the osmotic environment on its expression using northern blot analysis from immature embryos cultured in vitro and germinating mature seeds. Moreover, the cloning and characterization of its gene (referred to as sbp gene) allowed us to define various potential cis-acting elements within the promoter region to account for the observed strict seed-specific expression. The results described in this paper are consistent with a model in which ABA regulates, at least in part, expression of this gene. However, unlike most lea genes, ABA regulation of the sbp gene seems to occur in a very restricted fashion, being confined only to particular stages of embryo development. Such a strict spatial and temporal expression pattern is dependent on the osmotic environment of the developing embryos and on tissue-specific factors, presumably preventing biotin depletion in cells requiring this essential cofactor for basic metabolic activity. 相似文献
7.
Summary Somatic embryos of pineapple guava (Feijoa sellowiana Berg, Myrtaceae) were induced particularly well from the adaxial face of the cotyledons of zygotic embryos cultured on MS medium containing 1.0 mg/l 2,4-D and 0.3 M sucrose. Somatic embryos were never obtained from globular and heart-shaped zygotic embryos and embryos at the torpedo stage produced somatic embryos at lower frequencies than mature zygotic embryos. At the time of explantation, cotyledonary cells were rich in storage proteins and lipids but no starch was found. After the first 5 days of culture most of the reserves had been mobilized in cotyledons of germinating embryos, but were still present in large amounts in cotyledons undergoing embryogenie induction. In contrast to cotyledons following the normal pattern of development, cells of embryogenically-induced cotyledons accumulated starch, especially those cells not involved in the embryogenie process. Two patterns of somatic embryo differentiation were observed: (1) from single epidermal cells or (2) from groups of meristematic cells near the adaxial surface. Comparative observations on cotyledons from germinating embryos and those undergoing embryogenesis suggest that the meristematic layer arises as the result of successive divisions of cells that, under normal conditions, would form the palisade parenchyma. These were the only mesophyll cells that showed mitotic divisions during the normal development.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- FAA
formalin/acetic acid/ethyl alcohol
- PAS
periodic acid-Schiff 相似文献
8.
Raghavan V 《American journal of botany》2004,91(11):1743-1756
The relationship between cell expansion and cell cycling during somatic embryogenesis was studied in cultured bent-cotyledon-stage zygotic embryos of a transgenic stock of Arabidopsis thaliana harboring a cyclin 1 At:β-glucuronidase (GUS) reporter gene construct. In embryos cultured in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), following a brief period of growth by cell expansion, divisions were initiated in the procambial cells facing the adaxial side at the base of the cotyledons. Cell division activity later spread to almost the entire length of the cotyledons to form a callus on which globular and heart-shaped embryos appeared in about 10 d after culture. Anatomical and morphogenetic changes observed in cultured embryos were correlated with patterns of cell cycling by histochemical detection of GUS-expressing cells. Although early-stage somatic embryos did not develop further during their continued growth in the auxin-containing medium, maturation of embryos ensued upon their transfer to an auxin-free medium. In a small number of cultured zygotic embryos the shoot apical meristem was found to differentiate a leaf, a green tubular structure, or a somatic embryo. Contrary to the results from previous investigations, which have assigned a major role for the shoot apical meristem and cells in the axils of cotyledons in the development of somatic embryos on cultured zygotic embryos of A. thaliana, the present work shows that somatic embryos originate almost exclusively on the callus formed on the cotyledons. Other observations such as the induction of somatic embryos on cultured cotyledons and the inability of the embryo axis (consisting of the root, hypocotyl, and shoot apical meristem without the cotyledons) to form somatic embryos, reaffirm the important role of the cotyledons in somatic embryogenesis in this plant. 相似文献
9.
In the late globular proembryos, three regions could be identified, i. e. the cotyledon primordium, the epiphysis and the hypocotyl-hypophysis. In the cotyledon primordia, the mitotic frequency of the cells was comparitively high, the directions of the mitotic planes were mostly perpendicular to the long axis of the embryo, the size of the nucleolus was comparitively large, and the cytoplasm density was high. In the epiphysis region, however, the mitotic frequency of the cells was low, the size of the nucleolus was small, and as the first pair of leaf primordia appeared the mitotic frequency of the cells in that region began to increase. In the hypocotyls hypophysis region the mitotic frequency of the cells as well as the size of the nucleolus lied in between the corresponding values of those of the above two regions, the cytoplasm density was low and the size of the vacuoles was large. As the proembryo continued to develop the direction of the mitotic plane changed gradually, from mostly perpendicular to the long axis of the embryo to mainly inclined, or even parallel to that axis. As a result, the proembryo developed from a heart-shaped embryo into a torpedo-shaped embryo. After the first pair of leaf primordia appeared from the young embryo, the vacuoles in the cells of the cotyledons grew in size rapidly. About twenty to twenty five days after flowering, the starch grains, the protein bodies and the lipid granules began to accumulate in the cells of the cotyledons and gradually increased both in size as well as in quantity. About fifty days after flowering the diameter of the starch grains reached its maximum value of 6.2–7.0 μm, and decreased in value thereafter till the time of harvesting when most of the starch grains disappeared except those in the palisades. On the other hand, fifty to sixty days after flowering, the diameters of the lipid granules and of the protein bodies reached their maximum values of 5.4–7.0 μm and 6.2–7.0 μm, respectively. The observation revealed that the formation of the protein bodies was related to the vacules. 相似文献
10.
A DNA sequence representing the promoter region of the Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco) luminal binding protein PmBiP (PmBiPPro1) was isolated using inverse polymerase chain reaction (iPCR). Transient expression analysis of PmBiPPro1 fused to the beta-glucuronidase (GUS) reporter gene demonstrated that this promoter is functional in germinating Douglas-fir embryos. Transgenic Arabidopsis plants containing PmBiPPro1:GUS reporter gene constructs revealed strong staining associated with actively dividing/expanding cells and secretory tissues in developing seedlings. Wounding of cotyledons resulted in an increase in local staining associated with cells surrounding the wound site. Deletion analysis showed that elements necessary for basal-level expression reside within a -261 to +16 bp region, although upstream elements are necessary for higher-level expression in germinating Douglas-fir embryos, developing Arabidopsis seedlings and wounded cotyledons. Correlation of the observed expression pattern with the known function of BiP suggests that pathways controlling expression are highly conserved between angiosperms and gymnosperms. 相似文献
11.
M. Griga 《Biologia Plantarum》2002,45(2):173-182
The morphological and anatomical aspects of direct and indirect somatic embryogenesis in pea were described. Direct embryos were induced from shoot apical meristems of 3 to 5-d-old pea seedlings, embryogenic callus originated from immature pea zygotic embryos or shoot apices. Auxin (picloram, 2,4-dichlorophenoxyacetic acid) was necessary to induce somatic embryos. The developmental stages typical for pea zygotic embryos were detected. Globular and heartshaped somatic embryos were morphologically similar to their zygotic counterparts; in contrast, torpedo and cotyledonary somatic embryos displayed great morphological variation, which affected mainly cotyledons (size, shape, number). Based on anatomical sections, possible ways of somatic embryo formation and localization of initiation sites within primary explant tissue have been proposed. The multicellular origin of somatic embryos is supposed in both systems of pea somatic embryogenesis under investigation. 相似文献
12.
Improvement of pea biomass and seed productivity by simultaneous increase of phloem and embryo loading with amino acids
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Lizhi Zhang Matthew G. Garneau Rajtilak Majumdar Jan Grant Mechthild Tegeder 《The Plant journal : for cell and molecular biology》2015,81(1):134-146
The development of sink organs such as fruits and seeds strongly depends on the amount of nitrogen that is moved within the phloem from photosynthetic‐active source leaves to the reproductive sinks. In many plant species nitrogen is transported as amino acids. In pea (Pisum sativum L.), source to sink partitioning of amino acids requires at least two active transport events mediated by plasma membrane‐localized proteins, and these are: (i) amino acid phloem loading; and (ii) import of amino acids into the seed cotyledons via epidermal transfer cells. As each of these transport steps might potentially be limiting to efficient nitrogen delivery to the pea embryo, we manipulated both simultaneously. Additional copies of the pea amino acid permease PsAAP1 were introduced into the pea genome and expression of the transporter was targeted to the sieve element‐companion cell complexes of the leaf phloem and to the epidermis of the seed cotyledons. The transgenic pea plants showed increased phloem loading and embryo loading of amino acids resulting in improved long distance transport of nitrogen, sink development and seed protein accumulation. Analyses of root and leaf tissues further revealed that genetic manipulation positively affected root nitrogen uptake, as well as primary source and sink metabolism. Overall, the results suggest that amino acid phloem loading exerts regulatory control over pea biomass production and seed yield, and that import of amino acids into the cotyledons limits seed protein levels. 相似文献
13.
14.
Pattern Formation in the Arabidopsis Embryo Revealed by Position-Specific Lipid Transfer Protein Gene Expression 总被引:9,自引:6,他引:3
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Vroemen CW Langeveld S Mayer U Ripper G Jurgens G Van Kammen A De Vries SC 《The Plant cell》1996,8(5):783-791
During Arabidopsis embryogenesis, the zygote divides asymmetrically in the future apical-basal axis; however, a radial axis is initiated only within the eight-celled embryo. Mutations in the GNOM, KNOLLE, and KEULE genes affect these processes: gnom zygotes tend to divide symmetrically; knolle embryos lack oriented cell divisions that initiate protoderm formation; and in keule embryos, an outer cell layer is present that consists of abnormally enlarged cells from early development. Pattern formation along the two axes is reflected by the position-specific expression of the Arabidopsis lipid transfer protein (AtLTP1) gene. In wild-type embryos, the AtLTP1 gene is expressed in the protoderm and initially in all protodermal cells; later, AtLTP1 expression is confined to the cotyledons and the upper end of the hypocotyl. Analysis of AtLTP1 expression in gnom, knolle, and keule embryos showed that gnom embryos also can have no or reversed apical-basal polarity, whereas radial polarity is unaffected. knolle embryos initially lack but eventually form a radial pattern, and keule embryos are affected in protoderm cell morphology rather than in the establishment of the radial pattern. 相似文献
15.
Treml BS Winderl S Radykewicz R Herz M Schweizer G Hutzler P Glawischnig E Ruiz RA 《Development (Cambridge, England)》2005,132(18):4063-4074
During Arabidopsis embryo development, cotyledon primordia are generated at transition stage from precursor cells that are not derived from the embryonic shoot apical meristem (SAM). To date, it is not known which genes specifically instruct these precursor cells to elaborate cotyledons, nor is the role of auxin in cotyledon development clear. In laterne mutants, the cotyledons are precisely deleted, yet the hypocotyl and root are unaffected. The laterne phenotype is caused by a combination of two mutations: one in the PINOID (PID) gene and another mutation in a novel locus designated ENHANCER OF PINOID (ENP). The expression domains of shoot apex organising genes such as SHOOT MERISTEMLESS (STM) extend along the entire apical region of laterne embryos. However, analysis of pid enp stm triple mutants shows that ectopic activity of STM does not appear to cause cotyledon obliteration. This is exclusively caused by enp in concert with pid. In pinoid embryos, reversal of polarity of the PIN1 auxin transport facilitator in the apex is only occasional, explaining irregular auxin maxima in the cotyledon tips. By contrast, polarity of PIN1:GFP is completely reversed to basal position in the epidermal layer of the laterne embryo. Consequently auxin, which is believed to be essential for organ formation, fails to accumulate in the apex. This strongly suggests that ENP specifically regulates cotyledon development through control of PIN1 polarity in concert with PID. 相似文献
16.
Phosphoenolpyruvate carboxykinase in developing pea seeds is associated with tissues involved in solute transport and is nitrogen-responsive 总被引:1,自引:0,他引:1
The aim of this work was to investigate the occurrence of phosphoenolpyruvate carboxykinase (PEPCK) in developing pea (Pisum sativum) seeds in relation to their nitrogen supply. PEPCK was present throughout development, with the peak of PEPCK protein and activity in the seed coat and cotyledons preceding protein accumulation in the cotyledons. It showed a different developmental pattern from enzymes involved in amino acid metabolism (phosphoenolpyruvate carboxylase, glutamine synthetase and glutamate dehydrogenase). Immunolocalization showed that PEPCK was present in parts of the developing seed that are involved in the transport and metabolism of assimilates. Early in development, it was associated with the inner integument of the ovule, the endospermic cytoplasm and the outer cells of the embryo. In the middle of development, around the peak of activity, PEPCK was abundant at the outer surface of the developing cotyledons, in the embryonic axis and in the vasculature of the seed coat. Later in development, PEPCK was associated with the embryonic leaf primordia and meristem and cortex of the radicle. PEPCK protein was strongly induced in vitro in the seed coat by nitrate, ammonium and asparagine, in the cotyledons by asparagine and in planta by the supply of nitrogen, which led to an increase in asparagine secretion by empty seed coats. It is suggested that PEPCK is involved in the metabolism of nitrogenous solutes in developing pea seeds. 相似文献
17.
K.I. Aston G.P. Li B.A. Hicks B.R. Sessions A.P. Davis Q.A. Winger L.F. Rickords J.R. Stevens K.L. White 《Molecular reproduction and development》2009,76(5):471-482
Low developmental competence of bovine somatic cell nuclear transfer (SCNT) embryos is a universal problem. Abnormal placentation has been commonly reported in SCNT pregnancies from a number of species. The present study employed Affymetrix bovine expression microarrays to examine global gene expression patterns of SCNT and in vivo produced (AI) blastocysts as well as cotyledons from day‐70 SCNT and AI pregnancies. SCNT and AI embryos and cotyledons were analyzed for differential expression. Also in an attempt to establish a link between abnormal gene expression patterns in early embryos and cotyledons, differentially expressed genes were compared between the two studies. Microarray analysis yielded a list of 28 genes differentially expressed between SCNT and AI blastocysts and 19 differentially expressed cotyledon genes. None of the differentially expressed genes were common to both groups, although major histocompatibility complex I (MHCI) was significant in the embryo data and approached significance in the cotyledon data. This is the first study to report global gene expression patterns in bovine AI and SCNT cotyledons. The embryonic gene expression data reported here adds to a growing body of data that indicates the common occurrence of aberrant gene expression in early SCNT embryos. Mol. Reprod. Dev. 76: 471–482, 2009. © 2008 Wiley‐Liss, Inc. 相似文献
18.
Chun‐Ming Liu Sue Johnson Simona Di Gregorio Trevor L. Wang 《Genesis (New York, N.Y. : 2000)》1999,25(1):11-22
Angiosperms are divided into two distinct classes—the dicotyledons (dicots) and monocotyledons (monocots)—based in part on the number of cotyledons in mature embryos. In this paper, we describe single‐cotyledon pea mutants, termed sic (single cotyledon), all of which show a degree of fusion between the cotyledons. The fusion in sic1 is along the margin of one cotyledon and is less complete than in sic2 embryos, but the effects of the mutations are additive in the double mutant. Occasionally sic2 mutants will show fusion of the two cotyledons into one cylindrical embryo in which the shoot apex becomes surrounded by the cotyledons. Both sic1 and sic2 mutants produce fertile plants. In the sic3 embryo, a single cotyledon is generated under the shoot apex that breaks the vascular connection between root and shoot, causing embryo lethality. The pattern of cotyledon development in all these mutants is identified by in situ mRNA hybridization and antibody labeling, using the storage protein vicilin as a cotyledon‐specific marker. These patterns indicate that the joining of the cotyledons was due to zonal growth. The results indicate that there are genes in pea that influence the positioning and the morphology of the cotyledon. A model for cotyledon development in pea is proposed that is based on the regulation of the positioning of cell clusters by the sic genes. Dev. Genet. 25:11–22, 1999. © 1999 Wiley‐Liss, Inc. 相似文献
19.
The nature of the starch-synthesising plastids in developing pea (Pisum sativum L.) embryos has been investigated. Chlorophyll and starch were distributed throughout the cotyledon during development. Chlorophyll
content increased initially, then showed little change up to the point of drying out of the embryo. Starch content per embryo
increased dramatically throughout development. The chlorophyll content per unit volume was highest on the outer edge of the
cotyledon, while the starch content was highest on inner face. Nycodenz gradients, which fractionated mechanically-prepared
plastids according to their starch content, failed to achieve any significant separation of plastids rich in starch and ADP-glucose
pyrophosphorylase from those rich in chlorophyll and a Calvin-cycle marker enzyme, NADP-glyceraldehyde-3-phosphate dehydrogenase.
However, material that was not sufficiently dense to enter the gradients was enriched in activity of the Calvin-cycle marker
enzyme relative to that of ADP-glucose pyrophosphorylase. Nomarski and epi-fluorescence microscopy showed that intact, isolated
plastids, including those with very large starch grains, invariably contained chlorophyll in stromal structures peripheral
to the starch grain. We suggest that the starch-storing plastids of developing pea embryos are derived directly from chloroplasts,
and retain chloroplast-like characteristics throughout their development. Developing pea embryos also contain chloroplasts
which store little or no starch. These are probably located primarily on the outer edge of the cotyledons where there is sufficient
light for photosynthesis at some stages of development. 相似文献
20.
Histochemical localization of the expression pattern of a chimericgene (rolC-GUS) in zygotic embryo development in tobacco plantswas analysed. The results indicate that strong expression waslocalized mainly in the vascular cylinders of the cotyledonsand central axis of the hypocotyl. Quantitative analysis indicatedan increase of gene expression in embryos up to 20 d after pollination(DAP), but decreased at 30 DAP. Continuous increase of GUS activitywas recorded up to 12 d after imbibition (DAI) in germinatingseeds. The xylem cells were visualized following phloem differentiationin the cotyledons at 3 DAI.Copyright 1994, 1999 Academic Press Tobacco (Nicotiana tabacum cv. Samsun), transgenic plants, rolC promoter-GUS chimeric gene, germinating seeds, transition region, zygotic embryos 相似文献