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1.
Alonso-Perez E Suarez-Gestal M Calaza M Kwan T Majewski J Gomez-Reino JJ Gonzalez A 《Arthritis research & therapy》2011,13(3):R80
Introduction
Interferon regulatory factor 5 gene (IRF5) polymorphisms are strongly associated with several diseases, including systemic lupus erythematosus (SLE). The association includes risk and protective components. They could be due to combinations of functional polymorphisms and related to cis-regulation of IRF5 expression, but their mechanisms are still uncertain. We hypothesised that thorough testing of the relationships between IRF5 polymorphisms, expression data from multiple experiments and SLE-associated haplotypes might provide useful new information. 相似文献2.
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Several food borne outbreaks have highlighted the importance of Listeria monocytogenes to the public health and have been recognized as an emerging, important food borne pathogen, and a causative agent of listerioses. A number of genes are involved in the manifestation of Listeria virulence, hlyA is one among them. In the present study, 111 marine fish samples including prawns, finfishes and bivalves were screened for
the presence of Listeria species. The isolates were characterized biochemically and further L. monocytogenes were confirmed by polymerase chain reaction (PCR) technique using the hlyA gene as a tool to differentiate between L. monocytogenes and other non-pathogenic Listeria species. Out of 111 samples 5 (4.5%) samples were positive for L. monocytogenes. Among the three different types of samples bivalves were found to have maximum percent (12.5) of L. monocytogenes followed by prawns (3.84) and finfishes (2.9). Among all the 111 samples, 15 (13.51%) samples were positive for other Listeria species. It was observed that Listeria occurrence is more in shellfishes than in fin fishes. All the isolates were sensitive towards five different antibiotics
in sequence ciprofloxacin > sulphafurazole > norfloxacin > ampicillin and gentamicin. 相似文献
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Removal of heavy metals (Pb2+, Zn2+) from aqueous solution by dried biomass of Spirulina sp. was investigated. Spirulina rapidly adsorbed appreciable amount of lead and zinc from the aqueous solutions within 15 min of initial contact with the
metal solution and exhibited high sequestration of lead and zinc at low equilibrium concentrations. The specific adsorption
of both Pb2+ and Zn2+ increased at low concentration and decreased when biomass concentration exceeded 0.1 g l−1. The binding of lead followed Freundlich model of kinetics where as zinc supported Langmuir isotherm for adsorption with
their r
2
values of 0.9659 and 0.8723 respectively. The adsorption was strongly pH dependent as the maximum lead biosorption occurred
at pH 4 and 10 whereas Zn2+ adsorption was at pH 8 and 10. 相似文献
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Sanjay MK Srideshikan SM Vanishree VL Usha MS Raj AP Gaddad SM Shivannavar CT 《Indian journal of microbiology》2011,51(3):326-331
Superoxide dismutase has been discovered within the periplasm of several Gram-negative pathogens. We studied the Cu,Zn-SOD
enzyme in Escherichia coli isolated from clinical samples (stool samples) collected from patients suffering from diarrhea. Antibiogram studies of the
isolates were carried out to determine the sensitive and resistant strains. The metal co-factor present in the enzyme was
confirmed by running samples in native gels and inhibiting with 2 mM potassium cyanide. A 519 bp sodC gene was amplified from resistant and sensitive strains of Escherichia coli. Cloning and sequencing of the sodC gene indicated variation in the protein and amino acid sequences of sensitive and resistant isolates. The presence of sodC in highly resistant Escherichia coli isolates from diarrheal patients indicates that sodC may play role in enhancing the pathogenicity by protecting cells from exogenous sources of superoxide, such as the oxidative
burst of phagocytes. The presence of SodC could be one of the factors for bacterial virulence. 相似文献
9.
Jenher Lu Tzuchun Tsai Sielin Choo Shuyu Yeh Renbing Tang Anhang Yang Hsinyu Lee Jennkan Lu 《Journal of biomedical science》2011,18(1):73
Background
The tbx5 mutation in human causes Holt-Oram syndrome, an autosomal dominant condition characterized by a familial history of congenital heart defects and preaxial radial upper-limb defects. We report aberrant apoptosis and dormant cell growth over head, heart, trunk, fin, and tail of zebrafish embryos with tbx5 deficiency correspond to the dysmorphogenesis of tbx5 morphants. 相似文献10.
Two plant growth promoting rhizobacteria––Sinorhizobium meliloti RMP1 and Pseudomonas aeruginosa GRC2 were studied for integrated nutrient management to obtain improved yield of Brassica juncea. Low concentrations of urea and diammonium phosphate (DAP) stimulated the growth of both S. meliloti RMP1 and P. aeruginosa GRC2. 1 M of urea and 0.35 M of DAP was found lethal for RMP1, while 1.3 M and 0.37 M concentrations of urea and DAP proved to
be toxic for GRC2. Lc50 was observed as 0.49 M of urea and 0.15 M of DAP for RMP1, and 0.66 M urea and 0.18 M of DAP for GRC2. Urea and DAP adaptive variants of RMP1 and GRC2 was isolated. Adaptive bacterial variants had better growth rates at sub-lethal (Lc50) concentrations of urea and DAP as compared to non-adaptive variants. They also retained plant growth promoting attributes
similar to non adaptive variants. GRC2 and RMP1 did not affect the growth of each other and were chemotactically active for DAP, urea as well as root exudates of
B. juncea. Both the isolates colonized well in the rhizosphere of B. juncea, as their populations were recorded ≈5 log10 cfu g−1 after 120 days. Interestingly, the colonization ability was found even better when both strains were co-inoculated, as their
population was recorded in the range of ≈6 log10 cfu g−1 after 120 days. In field trials, application of RMP1 and GRC2 resulted in significant increase in biomass and yield of B. juncea as compared to control. However, yield was better with application of half dose and full dose of recommended fertilizers.
Interestingly, the biomass as well as yield improved further when both isolates were applied together along with half dose
of recommended fertilizers. 相似文献
11.
Radha Venkatesan Dhanasekaran Bodhini Nagarajan Narayani Viswanathan Mohan 《Indian journal of human genetics》2014,20(1):37-42
BACKGROUND:
The ABCC8 gene which encodes the sulfonylurea receptor plays a major role in insulin secretion and is a potential candidate for type 2 diabetes. The -3c → t (rs1799854) and Thr759Thr (C → T, rs1801261) single nucleotide polymorphisms (SNPs) of the ABCC8 gene have been associated with type 2 diabetes in many populations. The present study was designed to investigate the association of these two SNPs in an Asian Indian population from south India.MATERIALS AND METHODS:
A total of 1,300 subjects, 663 normal glucose tolerant (NGT) and 637 type 2 diabetic subjects were randomly selected from the Chennai Urban Rural Epidemiology Study (CURES). The -3c → t and Thr759Thr were genotyped in these subjects using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and a few variants were confirmed by direct sequencing.RESULTS:
The frequency of the ‘t’ allele of the -3c → t SNP was found to be 0.27 in NGT and 0.29 in type 2 diabetic subjects (P = 0.44). There was no significant difference in the genotypic frequency between the NGT and type 2 diabetic group (P = 0.18). Neither the genotypic frequency nor the allele frequency of the Thr759Thr polymorphism was found to differ significantly between the NGT and type 2 diabetic groups.CONCLUSION:
The -3c → t and the Thr759Thr polymorphisms of the ABCC8 gene were not associated with type 2 diabetes in this study. However, an effect of these genetic variants on specific unidentified sub groups of type 2 diabetes cannot be excluded. 相似文献12.
Pseudomonas syringae pv. tabaci causes wildfire disease in tobacco plants. The hrp pathogenicity island (hrp PAI) of P. syringae pv. tabaci encodes a type III secretion system (TTSS) and its regulatory system, which are required for pathogenesis in plants.
Three important regulatory proteins-HrpR, HrpS, and HrpL-have been identified to activate hrp PAI gene expression. The bacterial Lon protease regulates the expression of various genes. To investigate the regulatory
mechanism of the Lon protease in P. syringae pv. tabaci 11528, we cloned the lon gene, and then a Δlon mutant was generated by allelic exchange. lon mutants showed increased UV sensitivity, which is a typical feature of such mutants. The Δlon mutant produced higher levels of tabtoxin than the wild-type. The lacZ gene was fused with hrpA promoter and activity of β-galactosidase was measured in hrp-repressing and hrp-inducing media. The Lon protease functioned as a negative regulator of hrp PAI under hrp-repressing conditions. We found that strains with lon disruption elicited the host defense system more rapidly and strongly
than the wild-type strain, suggesting that the Lon protease is essential for systemic pathogenesis. 相似文献
13.
Ipsita Sahu B. K. Das Nilima Marhual M. Samanta B. K. Mishra A. E. Eknath 《Indian journal of microbiology》2011,51(4):515-520
In the present study the haemolytic and proteolytic activity of extracellular products (ECP) secreted from Aeromonas hydrophila (CAHH14 strain) were studied with respect to temperature and different time of incubation as well as its lethal toxicity
on rohu, Labeo rohita. The strain was isolated from Catla catla (showing abdominal dropsy symptom) collected from the pond of Central Institute of Freshwater Aquaculture (CIFA), Bhubaneswar,
India and was characterized on the basis of biochemical tests. The highest production of haemolysin was achieved when the
bacteria was grown at 35°C for 30 h. The proteolytic activity was found to be highest when the bacterium was grown at 30°C
for 36 h. The haemolytic and proteolytic toxin produced by Aeromonas hydrophila was found to be lethal to rohu (LD50 1.7 × 104 cfu/ml). The lethality of ECP was decreased by heating and completely inactivated by boiling at 100°C for 10 min. This indicates
that protease activity and haemolytic activity of A. hydrophila ECP was temperature dependant. 相似文献
14.
PKD2 gene encodes a critical cation channel protein that plays important roles in various developmental processes and is usually
evolutionarily conserved. In the present study, we analyzed the evolutionary patterns of PKD2 and its homologous genes (PKD2L1, PKD2L2) from nine mammalian species. In this study, we demonstrated the orthologs of PKD2 gene family evolved under a dominant purifying selection force. Our results in combination with the reported evidences from
functional researches suggested the entire PKD2 gene family are conserved and perform essential biological roles during mammalian evolution. In rodents, PKD2 gene family members appeared to have evolved more rapidly than other mammalian lineages, probably resulting from relaxation
of purifying selection. However, positive selection imposed on synonymous sites also potentially contributed to this case.
For the paralogs, our results implied that PKD2L2 genes evolved under a weaker purifying selection constraint than PKD2 and PKD2L1 genes. Interestingly, some loop regions of transmembrane domain of PKD2L2 exhibited higher P
N/P
S ratios than expected, suggesting these regions are more functional divergent in organisms and worthy of special attention.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Chun Ye, Huan Sun have contributed equally to this work. 相似文献
15.
Freitas JS Silva EM Leal J Gras DE Martinez-Rossi NM Dos Santos LD Palma MS Rossi A 《Cell stress & chaperones》2011,16(5):565-572
Heat shock proteins are molecular chaperones linked to a myriad of physiological functions in both prokaryotes and eukaryotes.
In this study, we show that the Aspergillus nidulans hsp30 (ANID_03555.1), hsp70 (ANID_05129.1), and hsp90 (ANID_08269.1) genes are preferentially expressed in an acidic milieu, whose expression is dependent on the palA
+ background under optimal temperature for fungal growth. Heat shock induction of these three hsp genes showed different patterns in response to extracellular pH changes in the palA+ background. However, their accumulation upon heating for 2 h was almost unaffected by ambient pH changes in the palA
− background. The PalA protein is a member of a conserved signaling cascade that is involved in the pH-mediated regulation
of gene expression. Moreover, we identified several genes whose expression at pH 5.0 is also dependent on the palA
+ background. These results reveal novel aspects of the heat- and pH-sensing networks of A. nidulans. 相似文献
16.
Hamid Galehdari Ali Mohammad Foroughmand Maryam Naderi Soorki Gholamreza Mohammadian 《Indian journal of human genetics》2009,15(1):9-12
BACKGROUND:
The common GJB2 gene mutation (35delG) has been previously reported from Iranian patients that were affected with nonsyndromic autosomal recessive deafness. We, therefore, for the first time, investigated the prevalence and frequency of the GJB2 gene mutation in the Iranian deaf population with Arabian origins.MATERIALS AND METHODs:
We amplified and sequenced the entire coding sequence of the GJB2 gene from 61 deaf patients and 26 control subjects.RESULT:
None of the analyzed samples revealed deafness-associated mutation.CONCLUSION:
This finding differs from several reports from Iran as we have focused on the GJB2 gene that possesses various mutations as the cause of congenital recessive deafness. 相似文献17.
Mark T Albrecht Wei Wang Olga Shamova Robert I Lehrer Neal L Schiller 《Respiratory research》2002,3(1):18
Background
Pseudomonas aeruginosa and Burkholderia cepacia infections of cystic fibrosis patients' lungs are often resistant to conventional antibiotic therapy. Protegrins are antimicrobial peptides with potent activity against many bacteria, including P. aeruginosa. The present study evaluates the correlation between protegrin-1 (PG-1) sensitivity/resistance and protegrin binding in P. aeruginosa and B. cepacia. 相似文献18.
Genetic and molecular analysis of the CLDN14 gene in Moroccan family with non-syndromic hearing loss
Majida Charif Redouane Boulouiz Amina Bakhechane Houda Benrahma Halima Nahili Abdelmajid Eloualid Hassan Rouba Mostafa Kandil Omar Abidi Guy Lenaers Abdelhamid Barakat 《Indian journal of human genetics》2013,19(3):331-336
BACKGROUND:
Hearing loss is the most prevalent human genetic sensorineural defect. Mutations in the CLDN14 gene, encoding the tight junction claudin 14 protein expressed in the inner ear, have been shown to cause non-syndromic recessive hearing loss DFNB29.AIM:
We describe a Moroccan SF7 family with non-syndromic hearing loss. We performed linkage analysis in this family and sequencing to identify the mutation causing deafness.MATERIALS AND METHODS:
Genetic linkage analysis, suggested the involvement of CLDN14 and KCNE1 gene in deafness in this family. Mutation screening was performed using direct sequencing of the CLDN14 and KCNE1 coding exon gene.RESULTS:
Our results show the presence of c.11C>T mutation in the CLDN14 gene. Transmission analysis of this mutation in the family showed that the three affected individuals are homozygous, whereas parents and three healthy individuals are heterozygous. This mutation induces a substitution of threonine to methionine at position 4.CONCLUSION:
These data show that CLDN14 gene can be i mplicated in the development of hearing loss in SF7 family; however, the pathogenicity of c.11C>T mutation remains to be determined. 相似文献19.
20.
Honghao Lv Zhiyuan Fang Limei Yang Yangyong Zhang Qingbiao Wang Yumei Liu Mu Zhuang Yuhong Yang Bingyan Xie Bo Liu Jisheng Liu Jungen Kang Xiaowu Wang 《BMC genomics》2014,15(1)