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1.
Oxygen transport to embryos in microdrop cultures 总被引:1,自引:0,他引:1
The standard method for culturing small preimplantation mammalian embryos is a system in which they are placed into microdrops of culture medium under oil. Thus the source of oxygen for the embryos lies beyond two liquid phases--medium and oil. If transport of oxygen is not sufficiently rapid to replace that consumed by the embryos, the medium could become depleted of oxygen. Over small distances, the dominant means by which oxygen is transported through liquid is by diffusion. Our calculations show that diffusion alone is sufficient to supply oxygen to mouse embryos; there is virtually no perturbation of the oxygen concentration when there are up to 10 embryos in the drop, and 50 embryos produce a drop in oxygen tension that is not large enough to have a deleterious effect. Furthermore, diffusion is probably not the dominant mechanism by which oxygen is transported to the embryos; on the scale of these microdrops, convection is faster and would serve to mix the drop so that anoxic regions cannot develop. Therefore, we conclude that even a relatively large number of embryos in a culture drop do not significantly deplete oxygen. 相似文献
2.
Seifert AW Chapman LJ 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2006,143(1):142-148
This paper quantifies the relationship between respiratory allocation (air vs. water) and the standard rate of metabolism (SMR) in the primitive air-breathing lungfish, Protopterus aethiopicus. Simultaneous measurements of oxygen consumed from both air and water were made to determine the SMR at ecologically relevant aquatic oxygen levels for juveniles 2 to 221 g. Total metabolic rate was positively correlated with body mass with a scaling exponent of 0.78. Aerial oxygen consumption averaged 98% (range=94% to 100%) of total respiratory allocation under low aquatic oxygen levels. Measurements of oxygen consumption made across a gradient of dissolved oxygen from normoxia to anoxia showed that P. aethiopicus maintains its SMR despite a change in respiratory allocation between water and air. 相似文献
3.
Jin B Kawai Y Hara T Takeda S Seki S Nakata Y Matsukawa K Koshimoto C Kasai M Edashige K 《Biology of reproduction》2011,85(4):834-847
The permeability of cells is important for cryopreservation. Previously, we showed in mice that the permeability to water and cryoprotectants of oocytes and embryos at early cleavage stages (early embryos) is low because these molecules move across the plasma membrane predominantly by simple diffusion through the lipid bilayer, whereas permeability of morulae and blastocysts is high because of a water channel, aquaporin 3 (AQP3). In this study, we examined the pathways for the movement of water and cryoprotectants in bovine oocytes/embryos and the role of AQP3 in the movement by determining permeability, first in intact bovine oocytes/embryos, then in bovine morulae with suppressed AQP3 expression, and finally in mouse oocytes expressing bovine AQP3. Results suggest that water moves through bovine oocytes and early embryos slowly by simple diffusion, as is the case in mice, although channel processes are also involved in the movement. On the other hand, water appears to move through morulae and blastocysts predominantly by facilitated diffusion via channels, as in mice. Like water, cryoprotectants appear to move through bovine oocytes/early embryos mostly by simple diffusion, but channel processes could also be involved in the movement of glycerol and ethylene glycol, unlike that in mice. In bovine morulae, although glycerol and ethylene glycol would move predominantly by facilitated diffusion, mostly through AQP3, as in mice, dimethylsulfoxide appears to move predominantly by simple diffusion, unlike in mice. These results indicate that permeability-related properties of bovine oocytes/embryos are similar to those of mouse oocytes/embryos, but species-specific differences do exist. 相似文献
4.
Oxidative phosphorylation-dependent and -independent oxygen consumption by individual preimplantation mouse embryos 总被引:1,自引:0,他引:1
The self-referencing electrode technique was employed to noninvasively measure gradients of dissolved oxygen in the medium immediately surrounding developing mouse embryos and, thereby, characterized changes in oxygen consumption and utilization during development. A gradient of depleted oxygen surrounded each embryo and could be detected >50 microm from the embryo. Blastocysts depleted the surrounding medium of 0.6+/-0.1 microM of oxygen, whereas early cleavage stage embryos depleted the medium of only 0.3+/-0.1 microM of oxygen, suggesting a twofold increase in oxygen consumption at the blastocyst stage. Mitochondrial oxidative phosphorylation (OXPHOS) accounted for 60-70% of the oxygen consumed by blastocysts, while it accounted for only 30% of the total oxygen consumed by cleavage-stage embryos. The amount of oxygen consumed by non-OXPHOS mechanisms remained relatively constant throughout preimplantation development. By contrast, the amount of oxygen consumed by OXPHOS in blastocysts is greater than that consumed by OXPHOS in cleavage-stage embryos. The amount of oxygen consumed by one-cell embryos was modulated by the absence of pyruvate from the culture medium. Treatment of one-cell embryos and blastocysts with diamide, an agent known to induce cell death in embryos, resulted in a decline in oxygen consumption, such that the medium surrounding dying embryos was not as depleted of oxygen as that surrounding untreated control embryos. Together these results validate the self-referencing electrode technique for analyzing oxygen consumption and utilization by preimplantation embryos and demonstrate that changes in oxygen consumption accompany important physiological events, such as development, response to medium metabolites, or cell death. 相似文献
5.
Fergusson-Kolmes L Podrabsky JE 《Journal of experimental zoology. Part A, Ecological genetics and physiology》2007,307(7):419-423
Embryos of the annual killifish Austrofundulus limnaeus can experience oxygen deprivation as part of their normal developmental environment. We exposed embryos to anoxia and monitored heart activity for 48 hr, and subsequent aerobic recovery from anoxia for 40 hr. Embryos were tested at four different developmental stages that differ in their tolerance of anoxia. Our results indicate that high tolerance of anoxia is associated with an arrest of heart contractility during the first 24 hr of anoxia. These embryos recover to normoxic levels of heart rate within 16 hr of aerobic recovery. In contrast, embryos from later developmental stages that have a highly reduced ability to survive long-term anoxia experience a severe bradycardia but not an arrest of heart rate. These data illustrate a new and potentially powerful model for investigating the effects of anoxia on the developing cardiovascular system in vertebrates. 相似文献
6.
Hiromi Miki Michiko Hirose Narumi Ogonuki Kimiko Inoue Fuyuko Kezuka Arata Honda Kazuyuki Mekada Ken‐Ichi Hanaki Hirotaka Iwafune Atsushi Yoshiki Fumitoshi Ishino Atsuo Ogura 《Genesis (New York, N.Y. : 2000)》2009,47(3):155-160
Mammalian androgenetic embryos can be produced by pronuclear exchange of fertilized oocytes or by dispermic in vitro fertilization of enucleated oocytes. Here, we report a new technique for producing mouse androgenetic embryos by injection of two round spermatid nuclei into oocytes, followed by female chromosome removal. We found that injection of round spermatids resulted in high rates of oocyte survival (88%). Androgenetic embryos thus produced developed into mid‐gestation fetuses at various rates, depending on the mouse strain used. All the fetuses examined maintained paternally specific genomic imprinting memories. This technique also enabled us to produce complete heterozygous F1 embryos by injecting two spermatids from different strains. The best rate of fetal survival (12% per embryos transferred) was obtained with C57BL/6 × DBA/2 androgenetic embryos. We also generated embryonic stem cell lines efficiently with the genotype of Mus musculus domesticus × M. m. molossinus. Thus, injection of two round spermatid nuclei followed by maternal enucleation is an effective alternative method of producing androgenetic embryos that consistently develop into blastocysts and mid‐gestation fetuses. genesis 47:155–160, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
7.
Guang‐Peng Li Kenneth L. White Kenneth I. Aston Thomas D. Bunch Brady Hicks Ying Liu Benjamin R. Sessions 《Molecular reproduction and development》2009,76(7):620-628
Four experiments were designed to examine the effects of colcemid, a microtubule assembly inhibitor, on the development of bovine nuclear transfer (NT) embryos in vitro and in vivo. Recipient oocytes matured at different times were exposed to colcemid. Approximately 80–93% of the exposed oocytes, with or without the first polar body (PB1), developed obvious membrane projections. In Experiment 1, oocytes matured for either 14–15 or 16–17 hr, treated with colcemid and used as recipient cytoplasm for NT resulted in over 40% blastocyst development. In Experiment 2, oocytes matured for 16–17 hr were treated with either 0.2 or 0.4 µg/ml colcemid for 2–3 or 5–6 hr, respectively. The percentages of blastocyst development (39–42%) were not statistically different among the different colcemid treatment groups, but were both higher (P < 0.05) than the control group (30%). Colcemid concentrations and length of colcemid treatment of oocytes did not affect their ability to support NT embryo development to the blastocyst and hatched blastocyst stages. Results from Experiment 3 indicate that semi‐defined medium increases morula and blastocyst development of NT embryos derived fromcolcemid‐treated oocytes under 5% CO2 in air atmosphere. In addition, cell numbers of blastocysts in colcemid‐treated groups were numerically higher than the control groups. After embryo transfer, higher (P < 0.05) pregnant rates were obtained from the colcemid‐treated group than the nontreated group. Five of 40 recipients (12.5%) which received embryos from colcemid‐treated oocytes delivered healthy calves, significantly higher than those recipients (3.3%) that received embryos derived from nontreated oocytes. Mol. Reprod. Dev. 76: 620–628, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
8.
Abstract. The supply of oxygen to respiring shoot tissue was investigated for three submerged macrophytes (Potamogeton crispus L., Egeria densa Planch, and Myriophyllum triphyllum Orchard). For all species, the response of oxygen uptake rates to the external O2 concentration was a rectangular hyperbola over the range 0–5.0 × 10?3m3 m?3. However, the response pattern for material with water-infiltrated lacunar airspaces was non-hyperbolic over this range. The change in response was interpreted as an increased substrate (O2) limitation, resulting from lower radial diffusion rates within the infiltrated material. Neither the uninfiltrated nor the infiltrated responses obeyed the linear and logarithmic formulae of the type observed for submerged macrophytes by earlier authors. These results suggest that the responses observed are affected by factors such as water velocity, internal restrictions to diffusion and the range of oxygen tensions investigated. Therefore, it is unlikely that one response formula can adequately account for the effects of oxygen concentration on submerged macrophyte oxygen uptake. The lacunar airspaces also represent a possible oxygen source for dark respiration. The consumption of oxygen from the airspaces was investigated by displacing the gas from the lacunae and measuring the subsequent increase in the rate of oxygen assimilation from the external liquid. Approximately 30% of the oxygen consumed by E. densa and P. crispus, and more than 40% of that consumed by M. triphyllum, was derived from the lacunar system. This O2 supply is a consequence of the higher oxygen concentration in the lacunae than in the external medium, due to the low solubility of oxygen in water. Storage of photosynthetically-produced oxygen in the lacunae could not be identified during a light/dark transient, due to rate changes caused by the effects of light on the respiratory metabolism. However, O2 partial pressure gradients artificially set up between the lacunae and water equilibrated within an hour, suggesting that excess oxygen would be lost to the water within this time. 相似文献
9.
An analytical model is developed that describes oxygen transport and oxygen consumption for small biological structures without
a circulatory system. Oxygen inside the organism is transported by diffusion alone. Oxygen transfer towards the organism is
retarded by a thin static fluid film at the surface of the organism. The thickness of this film models the outward water conditions,
which may range from completely stagnant water conditions to so-called well-stirred water conditions. Oxygen consumption is
concentration-independent above a specified threshold concentration (regulator behaviour) and is proportional to the oxygen
concentration below this threshold (conformer behaviour). The model takes into account shape and size of the organism and
predicts the transition from (pure) regulator behaviour to (pure) conformer behaviour, as well as the mean oxygen consumption
rate. Thereby the model facilitates a proper analysis of the physical constraints set on shape and size of organisms without
an active internal oxygen transport mechanism. This analysis is carried out in some detail for six characteristic shapes (infinite
sheet, cylinder and beam; finite cylinder, sphere and block). In a well-stirred external medium, a flattened shape appears
to be the most favourable for oxygen supply, while a compact shape (cube) is more favourable if the external medium is nearly
stagnant. The theoretical framework is applied to oxygen consumption data of eight teleost embryos. This reveals relative
insensitivity to external flow conditions in some species (e.g., winter flounder, herring), while others appear to rely on
external stirring for a proper oxygen supply (e.g., largemouth bass). Interestingly, largemouth bass is the only species in
our analysis that exhibits ‘fin-fanning’. 相似文献
10.
Vinita A. Hajeri Brent A. Little Mary L. Ladage Pamela A. Padilla 《Molecular biology of the cell》2010,21(5):712-724
Oxygen, an essential nutrient, is sensed by a multiple of cellular pathways that facilitate the responses to and survival of oxygen deprivation. The Caenorhabditis elegans embryo exposed to severe oxygen deprivation (anoxia) enters a state of suspended animation in which cell cycle progression reversibly arrests at specific stages. The mechanisms regulating interphase, prophase, or metaphase arrest in response to anoxia are not completely understood. Characteristics of arrested prophase blastomeres and oocytes are the alignment of condensed chromosomes at the nuclear periphery and an arrest of nuclear envelope breakdown. Notably, anoxia-induced prophase arrest is suppressed in mutant embryos lacking nucleoporin NPP-16/NUP50 function, indicating that this nucleoporin plays an important role in prophase arrest in wild-type embryos. Although the inactive form of cyclin-dependent kinase (CDK-1) is detected in wild-type–arrested prophase blastomeres, the inactive state is not detected in the anoxia exposed npp-16 mutant. Furthermore, we found that CDK-1 localizes near chromosomes in anoxia-exposed embryos. These data support the notion that NPP-16 and CDK-1 function to arrest prophase blastomeres in C. elegans embryos. The anoxia-induced shift of cells from an actively dividing state to an arrested state reveals a previously uncharacterized prophase checkpoint in the C. elegans embryo. 相似文献
11.
Biofilm‐related research using 96‐well microtiter plates involves static incubation of plates indiscriminate of environmental conditions, making oxygen availability an important variable which has not been considered to date. By directly measuring dissolved oxygen concentration over time we report here that dissolved oxygen is rapidly consumed in Staphylococcus epidermidis biofilm cultures grown in 96‐well plates irrespective of the oxygen concentration in the gaseous environment in which the plates are incubated. These data indicate that depletion of dissolved oxygen during growth of bacterial biofilm cultures in 96‐well plates may significantly influence biofilm production. Furthermore higher inoculum cell concentrations are associated with more rapid consumption of dissolved oxygen and higher levels of S. epidermidis biofilm production. Our data reveal that oxygen depletion during bacterial growth in 96‐well plates may significantly influence biofilm production and should be considered in the interpretation of experimental data using this biofilm model. Biotechnol. Bioeng. 2009;103: 1042–1047. © 2009 Wiley Periodicals, Inc. 相似文献
12.
Franchesca D. Houghton Jeremy G. Thompson Christopher J. Kennedy Henry J. Leese 《Molecular reproduction and development》1996,44(4):476-485
Oxygen consumption of preimplantation and early postimplantation mouse embryos has been measured using a novel noninvasive ultramicrofluorescence technique, based on an oil-soluble, nontoxic quaternary benzoid compound pyrene, whose fluorescence is quenched in the presence of oxygen. Pyruvate and glucose consumption, lactate production, and glycogen formation from glucose were also measured. Preimplantation mouse embryos of the strain CBA/Ca × C57BL/6 were cultured in groups of 10–30 in 2 μl of modified M2 medium containing 1 mmol l−1 glucose, 0 mmol l−1 lactate, and 0.33 mmol l−1 pyruvate, for between 4–6 hr. Day 6.5 and 7.5 embryos were cultured singly in 40 μl M2 medium for between 2–3 hr. Oxygen consumption was detected at all stages of development, including, for the first time, in the early postimplantation embryo. Consumption remained relatively constant from zygote to morula stages before increasing in the blastocyst and day 6.5–7.5 stages. When expressed as QO2 (μl/mg dry weight/hr), oxygen consumption was relatively constant from the one-cell to morula stages before increasing sharply at the blastocyst stage and declining to preblastocyst levels on days 6.5 and 7.5. Pyruvate was consumed during preimplantation stages, with glucose uptake undetectable until the blastocyst stage. Glucose was the main substrate consumed by the 6.5 and 7.5 day embryo. The proportions of glucose accounted for by lactate appearance were 81%, 86%, and 119% at blastocyst, day 6.5, and day 7.5 stages, respectively. The equivalent figures for glucose incorporated into glycogen were 10.36%, 0.21%, and 0.19%, respectively. The data are consistent with a switch from a metabolism dependent on aerobic respiration during early preimplantation stages to one dependent on both oxidative phosphorylation and aerobic glycolysis at the blastocyst stage, a pattern which is maintained on days 6.5 and 7.5. Our technique for measuring oxygen consumption may have diagnostic potential for selecting viable embryos for transfer following assisted conception techniques in man and domestic animals. © 1996 Wiley-Liss, Inc. 相似文献
13.
14.
Edashige K Tanaka M Ichimaru N Ota S Yazawa K Higashino Y Sakamoto M Yamaji Y Kuwano T Valdez DM Kleinhans FW Kasai M 《Biology of reproduction》2006,74(4):625-632
The cryosensitivity of mammalian embryos depends on the stage of development. Because permeability to water and cryoprotectants plays an important role in cryopreservation, it is plausible that the permeability is involved in the difference in the tolerance to cryopreservation among embryos at different developmental stages. In this study, we examined the permeability to water and glycerol of mouse oocytes and embryos, and tried to deduce the pathway for the movement of water and glycerol. The water permeability (L(P), microm min(-1) atm(-1)) of oocytes and four-cell embryos at 25 degrees C was low (0.63-0.70) and its Arrhenius activation energy (E(a), kcal/mol) was high (11.6-12.3), which implies that the water permeates through the plasma membrane by simple diffusion. On the other hand, the L(p) of morulae and blastocysts was quite high (3.6-4.5) and its E(a) was quite low (5.1-6.3), which implies that the water moves through water channels. Aquaporin inhibitors, phloretin and p-(chloromercuri) benzene-sulfonate, reduced the L(p) of morulae significantly but not that of oocytes. By immunocytochemical analysis, aquaporin 3, which transports not only water but also glycerol, was detected in the morulae but not in the oocytes. Accordingly, the glycerol permeability (P(GLY), x 10(-3) cm/min) of oocytes was also low (0.01) and its E(a) was remarkably high (41.6), whereas P(GLY) of morulae was quite high (4.63) and its E(a) was low (10.0). Aquaporin inhibitors reduced the P(GLY) of morulae significantly. In conclusion, water and glycerol appear to move across the plasma membrane mainly by simple diffusion in oocytes but by facilitated diffusion through water channel(s) including aquaporin 3 in morulae. 相似文献
15.
The influence of bacterial biomass on hydraulic properties of porous media (bioclogging) has been explored as a viable means for optimizing subsurface bioremediation and microbial enhanced oil recovery. In this study, we present a pore network simulator for modeling biofilm evolution in porous media including hydrodynamics and nutrient transport based on coupling of advection transport with Fickian diffusion and a reaction term to account for nutrient consumption. Biofilm has non‐zero permeability permitting liquid flow and transport through the biofilm itself. To handle simultaneous mass transfer in both liquid and biofilm in a pore element, a dual‐diffusion mass transfer model is introduced. The influence of nutrient limitation on predicted results is explored. Nutrient concentration in the network is affected by diffusion coefficient for nutrient transfer across biofilm (compared to water/water diffusion coefficient) under advection dominated transport, represented by mass transport Péclet number >1. The model correctly predicts a dependence of rate of biomass accumulation on inlet concentration. Poor network connectivity shows a significantly large reduction of permeability, for a small biomass pore volume. Biotechnol. Bioeng. 2011;108: 2413–2423. © 2011 Wiley Periodicals, Inc. 相似文献
16.
S. M. Powers H. M. Baulch S. E. Hampton S. G. Labou N. R. Lottig E. H. Stanley 《Biogeochemistry》2017,136(2):119-129
In lakes that experience seasonal ice cover, understanding of nitrogen–oxygen coupling and nitrification has been dominated by observations during open water, ice-free conditions. To address knowledge gaps about nitrogen–oxygen linkages under ice, we examined long-term winter data (30 + years, 2–3 sample events per winter) in 7 temperate lakes of forested northern Wisconsin, USA. Across lakes and depths, there were strong negative relationships between dissolved oxygen (DO) and the number of days since ice-on, reflecting consistent DO consumption rates under ice. In two bog lakes that routinely experience prolonged winter DO concentrations below 1.0 mg L?1, nitrate accumulated near the ice surface mainly in late winter, suggesting nitrification may depend on biogenic oxygen from photosynthesis. In contrast, within five oligotrophic-mesotrophic lakes, nitrate accumulated more consistently over winter and often throughout the water column, especially at intermediate depths. Exogenous inputs of nitrate to these lakes were minimal compared to rates of nitrate accumulation. To produce the nitrate via in-lake nitrification, substantial oxygen consumption by ammonium oxidizing microbes would be required. Among lakes and depths that had significant DO depletion over winter, the stoichiometric nitrifier oxygen demand ranged from 1 to 25% of the DO depletion rate. These estimates of nitrifier-driven DO decline are likely conservative because we did not account for nitrate consumed by algal uptake or denitrification. Our results provide an example of nitrification at temperatures < 5 degrees C having a substantial influence on ecosystem-level nitrogen and oxygen availability in seasonally-frozen, northern forested lakes. Consequently, models of under-ice dissolved oxygen dynamics may be advanced through consideration of nitrification, and more broadly, coupled nitrogen and oxygen cycling. 相似文献
17.
The rate of loss of water and the rate of uptake of oxygen were measured continuously throughout the development of Lucilia cuprina within the puparium. Changes in these parameters were correlated with changes observed in morphology of cuticles and respiratory structures during development.In development at 26°C, there is, at 20–22 hr after puparium formation a major loss of water by mechanical expulsion of moulting fluid chiefly through the posterior larval spiracles after the severing of the posterior larval tracheae. This loss of water is essential to survival and is followed by an extremely low rate of water loss attributed to slow diffusion of water through the resulting air gap between the pupal cuticle and the puparium. There is an increase in oxygen consumption during the pupal movements associated with the casting of the larval tracheae followed by a sharp reduction in oxygen consumption until the pupal horns are everted a short time later. This combination of physiological events enables development to proceed over a wide range of conditions in the puparial environment. 相似文献
18.
Skye N. Anderson Jason E. Podrabsky 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2014,184(3):355-370
Embryos of Austrofundulus limnaeus are exceptional in their ability to tolerate prolonged bouts of complete anoxia. Hypoxia and anoxia are a normal part of their developmental environment. Here, we exposed embryos to a range of PO2 levels at two different temperatures (25 and 30 °C) to study the combined effects of reduced oxygen and increased temperature on developmental rate, heart rate, and metabolic enzyme capacity. Hypoxia decreased overall developmental rate and caused a stage-specific decline in heart rate. However, the rate of early development prior to the onset of organogenesis is insensitive to PO2. Increased incubation temperature caused an increase in the developmental rate at high PO2s, but hindered developmental progression under severe hypoxia. Embryonic DNA content in pre-hatching embryos was positively correlated with PO2. Citrate synthase, lactate dehydrogenase, and phosphoenolpyruvate carboxykinase capacity were all reduced in embryos developing under hypoxic conditions. Embryos of A. limnaeus are able to develop normally across a wide range of PO2s and contrary to most other vertebrates severe hypoxia is not a teratogen. Embryos of A. limnaeus do not respond to hypoxia through an increase in the capacity for enzymatic activity of the metabolic enzymes lactate dehydrogenase, citrate synthase, or phosphoenolpyruvate carboxykinase. Instead they appear to adjust whole-embryo metabolic capacity to match oxygen availability. However, decreased DNA content in hypoxia-reared embryos suggests that cellular enzymatic capacity may remain unchanged in response to hypoxia, and the reduced capacity may rather indicate reduced cell number in hypoxic embryos. 相似文献
19.
Jeffrey M. Duerr Jason E. Podrabsky 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2010,180(7):991-1003
Diapausing embryos of the annual killifish Austrofundulus limnaeus have the highest reported anoxia tolerance of any vertebrate and previous studies indicate modified mitochondrial physiology
likely supports anoxic metabolism. Functional mitochondria isolated from diapausing and developing embryos of the annual killifish
exhibited VO2, respiratory control ratios (RCR), and P:O ratios consistent with those obtained from other ectothermic vertebrate species.
Reduced oxygen consumption associated with dormancy in whole animal respiration rates are correlated with maximal respiration
rates of mitochondria isolated from diapausing versus developing embryos. P:O ratios for developing embryos were similar to
those obtained from adult liver, but were diminished in mitochondria from diapausing embryos suggesting decreased oxidative
efficiency. Proton leak in adult liver corresponded with that of developing embryos but was elevated in mitochondria isolated
from diapausing embryos. In metabolically suppressed diapause II embryos, over 95% of the mitochondrial oxygen consumption
is accounted for by proton leak across the inner mitochondrial membrane. Decreased activity of mitochondrial respiratory chain
complexes correlates with diminished oxidative capacity of isolated mitochondria, especially during diapause. Respiratory
complexes exhibited suppressed activity in mitochondria with the ATP synthase exhibiting the greatest inhibition during diapause
II. Mitochondria isolated from diapause II embryos are not poised to produce ATP, but rather to shuttle carbon and electrons
through the Kreb’s cycle while minimizing the generation of a proton motive force. This particular mitochondrial physiology
is likely a mechanism to avoid production of reactive oxygen species during large-scale changes in flux through oxidative
phosphorylation pathways associated with metabolic transitions into and out of dormancy and anoxia. 相似文献
20.
The oxygen consumption of single cysts (90–110 /tg dry wt) was measured with an oxygen electrode microrespirometer. The mean oxygen consumption of nine cysts after 7 days in tap-water, was 0–48 + 0–05 mm3 02 mg dry wt-1 h-1. After transfer to potato root diffusate for 1 day the mean oxygen consumption of the same cysts showed a significant increase to 159±7% of the rate recorded before they were removed from water. After 3 and 7 days in diffusate the corresponding means were 131±9% and 127±12% respectively. Cysts that remained in water throughout the experiments did not show any significant change in their oxygen consumption from the rate recorded after 7 days. The initial increase in oxygen uptake after the addition of diffusate was shown not to be due to the presence of microorganisms. Comparison of hatching data with the changes in oxygen consumption of similar cysts after 24 h in diffusate suggests that the increased oxygen uptake cannot be attributed solely to locomotor activity of the juveniles during the hatching process. The increased rate of respiration may precede other known changes that follow after the juveniles within a cyst are stimulated to hatch. 相似文献