芽前胡的化学成分

从成都产芽前胡Peucedanum turgeniifolium Wolff.中分离鉴定了12个化合物,分别为香豆素化合物佛手柑内酯(bergapten)(1),异欧芹属乙素(isoimperatorin)(2),(±)diisovaleryl-cis-khellactone(3),(±)dihydrosamidin(4),(±)peuformosin(5),(±)cis-khellactone(6),8-(2’,3’-二羟基,3’-甲基-丁基)-伞形花内酯[8-(2’3’-dihydroxy,3’-methyl-butyl)-umbelliferone](7),(±)selinidin(8),turgeniifolin A(9)以及非香豆素化合物硬脂酸(stearic acid),β-谷甾醇(β-sitosterol),甘露醇(d-mannitol)。     

土木香化学成分的研究

采用硅胶柱色谱、制备薄层色谱、制备高效液相、葡聚糖凝胶SephadexLH-20等方法进行分离纯化,根据光谱数据进行结构鉴定。从乙醇提取物中分离得到了8个化合物,分别鉴定为：异土木香内酯（1）,11,13-二氢异土木香内酯（2）,土木香内酯（3）,β-谷甾醇（4）,3-hydroxy-11,13-dihydroisoalantolactone（5）,macrophyllilae-toneE（6）,HIS-12-en-18-H-3-O-β-D-glucopyranoside（7）,caffeic acid anhydride（8）。化合物7和8系首次从该属植物中分离得到。     

木奶果茎叶的化学成分研究

采用常压柱色谱和重结晶相结合的分离方法,从木奶果的茎叶提取物的乙酸乙酯部分中分离得到8个化合物,通过波谱方法及与已知样品对照的手段鉴定它们为(2S,3S,4R)-2-[(2R)-2-hydroxytetracosanoyl-amino]-1,3,4-octadecanetriol(1),Aralia cerebroside(2),(24S)-24-ethylcholesta-3β,5α,6β-triol(3),Stigmast-4-en-6β-ol-3-one(4),7-oxo-β-sitosterol(5),7α-methoxy-sigmast-5-en-3β-ol(6),β-sitosterol(7),daucosterol(8)。化合物1-6为首次从该种植物中发现的。     

中华小苦荬萜类化学成分的研究(英文)

从中华小苦荬全草的乙酸乙酯提取物中分离得到8个萜类化合物,通过波谱方法及文献对照分别鉴定为β-香树脂素(1),3β-羟基-20(30)-蒲公英甾烯(2),熊果-12-烯-3β-醇(3),羽扇豆醇(4),10-羟基艾里莫芬-7(11)-烯-12,8α-内酯(5),乌苏-12,20(30)-二烯-3β,28-二醇(6),3β,8α-二羟基-6β-当归酰基艾里莫芬-7(11)-烯-12,8β-内酯(7)和乌苏酸(8),化合物1～8均首次从该植物中分离得到。     

杨桃根水提取物化学成分及生物活性的研究

为探究杨桃根中具备降血糖功能的活性成分,采用色谱分离方法从其水提取物中分离得到9个化合物,应用波谱技术对它们的化学结构进行了鉴定,分别命名为prismaconnatoside(1),tarennanosides A(2),(+)-catechin(3),fernandoside(4),7a-[(β-glucopyranosyl) oxy]-lyoniresinol(5),(+)-lyoniresinol 3α-O-β-D-glucopyranoside(6),(-)-lyon-iresinol 3α-O-β-D-glucopyranoside(7),(-)-5’-methoxy-isolariciresinol 3α-O-β-D-glucopyranoside(8)和正辛烷(9)。其中化合物1,2,4,5均为首次从杨桃中分离得到,化合物3为首次从杨桃根中分离得到。对部分单体化合物进行了体外α-葡萄糖苷酶和DPP-IV酶抑制活性测试,化合物1具有较强的α-葡萄糖苷酶抑制活性。     

马比木根的化学成分研究

通过硅胶、MCI和Sephadex LH-20反复柱层析、纯化,从马比木根的甲醇提取物中分离得到14个化合物,运用现代波谱技术鉴定为β-谷甾醇(1),β-胡萝卜苷(2),7-酮基-β-谷甾醇(3),β-sitosteryl-3-O-β-D-glucopyranoside-2'-O-palmitate(4),5α,6β-二羟基胡萝卜苷(5),羽扇豆醇(6),3-乙酰氧基-12-齐墩果烯-28醇(7),喜树碱(8),9-甲氧基喜树碱(9),10-羟基喜树碱(10),9-methoxy-mappicine-20-O-β-D-glucopyranoside(11),mappicine-20-O-β-D-glucopyranoside(12),(3S)-pumiloside(13),(-)-(3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(14)。化合物1~7、9~14为首次从该植物中分离得到。     

瑞香狼毒化学成分研究

从瑞香狼毒95%乙醇提取物中分离得到了17个化合物,根据理化性质和波谱数据鉴定为4’-甲氧基-7-羟基黄酮(1),山奈酚(2),5,4’-二羟基-7-甲氧基二氢黄酮(3),4’,5,7-三羟基黄烷酮(4),3,5,7-三羟基黄烷酮(5),山奈酚-7-O-β-D-葡萄糖苷(6),α-香树酯醇(7),(24R)-5α-豆甾-7,22(E)-二烯-3α-醇(8),狼毒色原酮(9),伞形花内酯(10),东莨菪素(11),异东莨菪素(12),狼毒素(13),瑞香内酯(14),异茴芹香豆素(15),β-谷甾醇(16),瑞香素乙(17)和β-胡萝卜甙(18)。其中化合物1-8为首次从该植物中分离得到。     

云南割舌树的化学成分

从云南割舌树（Walsura yunnanensis C.Y.Wu.)树皮的乙醇提取物中分离鉴定了12个化合物,它们分别是walsurol(1),tocopherol(2),sitoindoside Ⅰ（3）,3β-stigmast-5-en-3-yl-β-D-xylopyranoside(4),stigmast-4-en-6β-ol-3-one(5),7-oxositosterol(6),3β-hydroxy-5α,8α-epidioxyergosta-6,22-diene(7),(-)epicatechin(8),3,5-dihydroxy-4-methoxylphenylethanol(9),间三甲氧基苯,（β－谷甾醇和胡萝卜甙,新化合物1命名为割舌醇（walsurol)。     

罗汉松内生真菌Pestalotiopsis heterocornis的代谢产物研究

从罗汉松内生真菌Pestalotiopsis heterocornis的发酵培养液中分离得到10个代谢产物,应用质谱、核磁共振等现代波谱学方法鉴定为jesterone(1),hydroxy-jesterone(2),ambuic acid(3),6β-羟基-豆甾-4-烯-3-酮(4),(24S)-麦角甾-5-烯-3β,7α-二醇(5),7,22-二烯-3β,5α,7β-三羟基-麦角甾醇(6),麦角甾-7,22-二烯-3-酮(7),(4E,8E,2S,3R,2'R)-N-2'-羟基棕榈酰-9-甲基-4,8-sphingadienin(8),鲨肝醇(9),棕榈酸(10)。以上化合物均为首次从内生真菌P. heterocornis的代谢产物中分离得到,化合物4,6,7为首次从拟盘多毛孢属真菌代谢产物中分离得到。     

珍珠菜的化学成分研究(英文)

从珍珠菜(Lysimachia clethroides)根部分离得到8个化合物,通过波谱数据结合理化性质分别鉴定为山奈素-3-O-β-D-吡喃葡萄糖苷(1),山奈素-3-O-β-D-(2-O-β-D-吡喃葡萄糖)吡喃葡萄糖苷(2),(+)-儿茶素(3),(-)-表儿茶素(4),(+)-没食子儿茶素(5),(-)-表没食子儿茶素(6),(E)-2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucopyranoside(7)和2,3,5,4′-tetrahydroxystilbene-3-O-β-D-glucopyranoside(8)。所有化合物均为首次从该植物中分离得到。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细