共查询到20条相似文献,搜索用时 9 毫秒
1.
Defense-related terpenoid biosynthesis in conifers is a dynamic process closely associated with specialized anatomical structures that allows conifers to cope with attack from many potential pests and pathogens. The constitutive and inducible terpenoid defense of conifers involves several hundred different monoterpenes, sesquiterpenes and diterpenes. Changing arrays of these many compounds are formed from the general isoprenoid pathway by activities of large gene families for two classes of enzymes, the ter... 相似文献
2.
萜类化合物在植物间接防御中的作用 总被引:1,自引:0,他引:1
植物受到虫害后释放萜类化合物吸引害虫天敌的间接防御是一种新颖且环保的抗虫模式。植物受到害虫危害等因素诱导后做出响应,在体内经过一系列复杂调控,释放萜类物质,害虫天敌顺着这些萜类物质找到害虫,通过捕食或寄生于害虫体内的方法来消灭害虫。在实际应用中,可考虑利用直接和间接防御相结合的防治方法。 相似文献
3.
Metabolic Engineering of Terpenoid Biosynthesis in Plants 总被引:4,自引:0,他引:4
Asaph Aharoni Maarten A. Jongsma Tok-Yong Kim Man-Bok Ri Ashok P. Giri Francel W. A. Verstappen Wilfried Schwab Harro J. Bouwmeester 《Phytochemistry Reviews》2006,5(1):49-58
Metabolic engineering of terpenoids in plants is a fascinating research topic from two main perspectives. On the one hand, the various biological activities of these compounds make their engineering a new tool for improving a considerable number of traits in crops. These include for example enhanced disease resistance, weed control by producing allelopathic compounds, better pest management, production of medicinal compounds, increased value of ornamentals and fruit and improved pollination. On the other hand, the same plants altered in the profile of terpenoids and their precursor pools make a most important contribution to fundamental studies on terpenoid biosynthesis and its regulation. In this review we describe our recent results with terpenoid engineering, focusing on two terpenoid classes the monoterpenoids and sesquiterpenoids. The emerging picture is that engineering of these compounds and their derivatives in plant cells is feasible, although with some requirements and limitations. For example, in terpenoid engineering experiments crucial factors are the subcellular localisation of both the precursor pool and the introduced enzymes, the activity of endogenous plant enzymes which modify the introduced terpenoid skeleton, the costs of engineering in terms of effects on other pathways sharing the same precursor pool and the phytotoxicity of the introduced terpenoids. Finally, we will show that transgenic plants altered in their terpenoid profile exert novel biological activities on their environment, for example influencing insect behaviour.A. Aharoni is an Incumbent of the Adolfo and Evelyn Blum Career Development chair 相似文献
4.
植物萜类次生代谢及其调控 总被引:5,自引:0,他引:5
植物次生代谢在植物生长发育、环境适应、抵御病虫害等方面发挥着重要作用,这些天然产物组成地球上最丰富的有机化合物的宝库.萜类是植物代谢产物中种类最多的一类,具有重要的生理和生态功能,一些成分还有应用价值.近十几年来,人们在萜类化合物的分离、鉴定、应用、生物合成、相关基因与基因族、酶蛋白结构和功能、代谢调控以及代谢工程等各方面取得了重大进展.本文概述了植物萜类化合物代谢及其调控领域的研究进展与发展趋势. 相似文献
5.
6.
7.
8.
The seeds of 20 legume species were grown in the greenhouse or in growth chambers at different temperatures. Under warm temperature conditions (above 15 °C), six species, pea (Pisum sativum), broadbean (Vicia faba), chickpea (Cicer arietinum), lentil (Lens culinaris), wild lupine (Lupinus latifolius), and soybean (Glycine max), formed cavities in the vascular cylinder of their primary roots, which in turn became filled by the ingrowth of specialized parenchyma cells (SP cells). When these species were grown at low temperature (below 15 °C), however, a “normal” vascular cylinder formed in the primary roots with late-maturing metaxylem vessel members differentiating in the center. These species were all cool-season legumes except soybean, a warm-season legume, which sometimes also formed cavities and SP cell ingrowths. The occurrence of cavities and SP cells therefore was restricted to the cool-season legumes (except soybean) when they were grown under warm temperature conditions. The position and size of cavities varied among these species. Pea and broadbean usually formed large, axially elongated cavities in the central vascular cylinder, or in the xylem poles. Others formed smaller cavities of various lengths. 相似文献
9.
Background
The development and simulation of dynamic models of terpenoid biosynthesis has yielded a systems perspective that provides new insights into how the structure of this biochemical pathway affects compound synthesis. These insights may eventually help identify reactions that could be experimentally manipulated to amplify terpenoid production. In this study, a dynamic model of the terpenoid biosynthesis pathway was constructed based on the Hybrid Functional Petri Net (HFPN) technique. This technique is a fusion of three other extended Petri net techniques, namely Hybrid Petri Net (HPN), Dynamic Petri Net (HDN) and Functional Petri Net (FPN). 相似文献10.
11.
12.
A latex pellet fraction from Euphorbia lathyris incorporates mevalonate into triterpenols and their fatty acid esters. Conditions for improved incorporation were determined. CaCl2 or CaCl2 plus MnCl2 stimulated biosynthesis, and the metal ion chelator, ethylenebis(oxyethylenenitrilo)tetraacetic acid (EGTA) enhanced stimulation. Ethylenediaminetetraacetic acid was almost as effective as EGTA, but phthalic acid and citric acid were relatively poor stimulators. The concentration of the Ca2+-EGTA complex was directly measured, and the incorporation data are best fitted by a curve that shows that the receptor for the complex is saturable. In the presence of the metal-chelate complex, the addition of fructose, 1,6-bisphosphate plus aldolase (triose-P) or malate provided additional stimulation. Incorporation was maximum at 40 micromolar R-mevalonate, and inhibition occurred at higher concentrations. The apparent Km for R-mevalonate was 15 micromolar. Under improved reaction conditions, the rate of triterpenoid biosynthesis from mevalonate is 25 times faster than was previously observed (GJ Piazza, EJ Saggese, KM Spletzer [1987] Plant Physiol 83: 177-180). 相似文献
13.
14.
In the laminae of Digitalis, most of the digitoxin present isfound in the mesophyll. A new method for determining the amountof digitoxin biosynthesis using a digitoxin antibody was devisedto estimate this activity in isolated mesophyll cells and culturedcells. Isolated mesophyll cells showed significant activity,which suggests that the site of biosynthesis and the accumulationof cardenolides in a lamina of Digitalis is mainly in the mesophyllcells. Of five liquid cultures of D. purpurea; green shoot-formingcultures, white shoot-forming cultures, root-forming cultures,undifferentiated green cells and undifferentiated white cells,the green shoot-forming cultures had the highest activity. Thewhite shoot-forming cultures had about one-third the activityof the green shoot-forming cultures, and the other three cultureshad very low activity. No stimulatory effect of light was foundduring the 48-h incubation. (Received January 19, 1984; Accepted June 8, 1984) 相似文献
15.
16.
17.
18.
The phloem of very young petioles of Nelumbo nucifera Gaertn.(Nelumbium speciosum Willd.) was studied with the light microscope.The elongated, mature sieve elements contain slime, plugs, strands,and numerous plastids. Some sieve elements remain nucleatedfor a brief period even after the sieve plates are well developed.The companion cells numbering 814 undergo disintegrationbefore the elongation of the ontogenetically related sieve elementis completed. They are uninucleate to begin with but later becomebinucleate and finally degenerate and obliterate. The variousstages in their ontogeny and disintegration are described. Ofthe very few specialized phloem parenchyma cells present, someare associated with sieve elements. They have slime body-likestructures, and plastid-like bodies which group together andeventually disintegrate. 相似文献
19.
20.
Siyuan Guo Jiahao Liang Zhiwei Deng Ziqing Lu Minghui Fu Jianyu Su 《Current issues in molecular biology》2022,44(9):4197
Cinnamomum burmannii is a cinnamomum plant rich in natural D-borneol. Natural D-borneol is a bicycle monoterpenoid compound widely used in the food, pharmaceutical, and cosmetic industries. Therefore, analyzing the biosynthesis mechanism of natural D-borneol in C. burmannii at the molecular level is helpful for directional breeding in the future and further development and utilization of C. burmannii and its related gene resources. In our study, 76 genes related to terpene metabolism were analyzed through third-generation sequencing and second-generation sequencing. Of these genes, 57 were associated with the synthesis of the terpenoid skeleton, and 19 belonged to terpenoid synthase, including four monoterpenoid synthases, seven sesquiterpenoid synthases, and eight diterpenoid synthases. Two genes in diterpenoid synthase were differentially expressed in high D-borneol and low D-borneol plants. It was speculated that these two genes might be related to D-borneol synthesis. How these two genes participate in the synthesis of D-borneol needs further study. 相似文献