共查询到20条相似文献,搜索用时 31 毫秒
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Yanping Zhang Youhuang Bai Jian Han Ming Chen Emrul Kayesh Weibing Jiang Jinggui Fang 《Journal of plant physiology》2013
We predicted 262 potential MicroRNAs (miRNAs) belonging to 70 miRNA families from the peach (Prunus persica) genome and two specific 5′ and 3′ miRNA rapid amplification of cDNA ends (miR-RACE) PCR reactions and sequence-directed cloning were employed to accurately validate 61 unique P. persica miRNAs (Ppe-miRNAs) sequences belonging to 61 families comprising 97 Ppe-miRNAs. Validation of the termini nucleotides in particular can define the real sequences of the Ppe-miRNAs on peach genome. Comparison between predicted and validated Ppe-miRNAs through alignment revealed that 43 unique orthologous sequences were identical, while the remaining 18 exhibited some divergences at their termini nucleotides. Quantitative real-time polymerase chain reaction (qRT-PCR) was further employed to analyze the expression of all the 61 miRNAs and 10 putative targets of 8 randomly selected Ppe-miRNAs in peach leaves, flowers and fruits at different stages of development, where both the miRNAs and the putative target genes showed tissue-specific expression. 相似文献
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Plants have evolved diverse mechanism to recognize pathogen attack and triggers defense responses. These defense responses
alter host cellular function regulated by endogenous, small, non-coding miRNAs. To understand the mechanism of miRNAs regulated
cellular functions during stem rust infection in wheat, we investigated eight different miRNAs viz. miR159, miR164, miR167,
miR171, miR444, miR408, miR1129 and miR1138, involved in three different independent cellular defense response to infection.
The investigation reveals that at the initiation of disease, accumulation of miRNAs might be playing a key role in hypersensitive
response (HR) from host, which diminishes at the maturation stage. This suggests a possible host-fungal synergistic relation
leading to susceptibility. Differential expression of these miRNAs in presence and absence of R gene provides a probable explanation of miRNA regulated R gene mediated independent pathways. 相似文献
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盐胁迫下大豆根组织定量PCR分析中内参基因的选择 总被引:1,自引:0,他引:1
实时荧光定量PCR已广泛用于基因表达的分析, 适当的内参基因选择是获得准确分析结果的关键。在大豆(Glycine max)分子生物学研究中, 逆境响应基因和microRNA (miRNA)表达的内参辅助检测基因均有哪些目前尚不清楚。该研究选用不同盐梯度和时间点组合处理的大豆根组织为材料, 对已报道的其它条件下表达相对稳定的内参基因(ACT、ACT2/7、CYP2、ELF1A、ELF1B、F-Box、TUA和UBC2)以及miRNA内参基因(U6、miR1515a、miR1520c、miR1520d、miR171a和miR171b)的表达情况进行了全面检测; 并采用Δ-Ct、Bestkeeper、NormFinder和Genorm四种方法对检测结果进行了综合分析, 发现ELF1B和CYP2适合作为大豆根系盐胁迫响应基因研究的内参基因, miR1515a和U6适合作为盐胁迫下大豆根组织miRNA研究的内参。上述研究结果为大豆盐胁迫响应基因和miRNA表达及其进一步的功能研究奠定了基础。 相似文献
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Identification and functional characterization of soybean root hair microRNAs expressed in response to Bradyrhizobium japonicum infection
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Oswaldo Valdés‐López Nhung T. Hoang Jixian Zhai Jun Wang Marc Libault Laurent Brechenmacher Seth Findley Trupti Joshi Lijuan Qiu D. Janine Sherrier Tieming Ji Blake C. Meyers Dong Xu Gary Stacey 《Plant biotechnology journal》2016,14(1):332-341
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Sebastian Gasparis Yuliya Yanushevska Anna Nadolska-Orczyk 《Acta Physiologiae Plantarum》2017,39(10):236
As an essential regulatory component in plants, microRNAs (miRNAs) have been intensively studied over the past decade. Although hundreds of miRNAs have been identified and analyzed in many important crops and model plants, very little is known about the function of common wheat (Triticum aestivum L.) miRNAs. In this study, we performed computational prediction of novel wheat miRNAs based on BLAST searches of the expressed sequence tag database. The expression profiles of all miRNAs were performed for both vegetative and reproductive tissues to identify developmentally regulated miRNAs. A total of 19 new miRNAs belonging to 12 MIR families were identified using stringent criteria for miRNA annotation. For all of the miRNAs, the secondary structures of their precursor sequences were predicted. Two pairs of distinct miRNAs were found to be located on the same precursor. The predicted miRNAs were experimentally verified by a stem-loop qRT-PCR-based assay. The expression profiles were performed in both vegetative and reproductive tissues to find the potential correlations between the developmental phase and miRNA activity. Thirteen out of 19 miRNAs were upregulated at certain phases of plant development, and three of them (miR319, miR395, and miR171) showed the greatest expression in young spikes during microsporogenesis. Our results provide useful information for future studies of miRNA-mediated regulation of flower and grain development in wheat. 相似文献
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Wenzheng Yang Xin Liu Jianguang Zhang Junli Feng Chao Li Jishuang Chen 《Molecular biology reports》2010,37(7):3081-3087
Potato (Solanum tuberosum) is an important crop around the world, and accounts for a significant amount of the food consumed by humans. However, little
information is available about potato miRNAs which play important regulatory roles in plant growth and development. In the
present study, computational prediction of potential miRNAs from potato revealed 71 miRNAs belonging to 48 families. Amongst
these 71 mRNAs, 65 were predicted for the first time. Most potato miRNA families have one to three members, and sequence analysis
showed that the candidate pre-miRNA sequences varied from 48 to 224 bp in length. To verify the predicted miRNAs, specific
stem-loop RT primers were designed and real-time PCR assays were used to profile the expression levels of seven miRNAs from
different tissues of potato. The results showed that all the selected miRNAs were successfully amplified. Most of them had
their highest expression levels in leaves, and the lowest levels in the stem, while miR159 and miR164 presented a different
expression pattern. The specific expression levels of each miRNAs in the tested tissues may be related to their particular
functions in regulating potato vegetative growth and organ development. 相似文献
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microRNAs (miRNAs) are a class of negative regulators that take part in many processes such as growth and development, stress responses, and metabolism in plants. Recently, miRNAs were shown to function in plant nutrient metabolism. Moreover, several miRNAs were identified in the response to nitrogen (N) deficiency. To investigate the functions of other miRNAs in N deficiency, deep sequencing technology was used to detect the expression of small RNAs under N-sufficient and -deficient conditions. The results showed that members from the same miRNA families displayed differential expression in response to N deficiency. Upon N starvation, the expression of miR169, miR171, miR395, miR397, miR398, miR399, miR408, miR827, and miR857 was repressed, whereas those of miR160, miR780, miR826, miR842, and miR846 were induced. miR826, a newly identified N-starvation-induced miRNA, was found to target the AOP2 gene. Among these N-starvation-responsive miRNAs, several were involved in cross-talk among responses to different nutrient (N, P, S, Cu) deficiencies. miR160, miR167, and miR171 could be responsible for the development of Arabidopsis root systems under N-starvation conditions. In addition, twenty novel miRNAs were identified and nine of them were significantly responsive to N-starvation. This study represents comprehensive expression profiling of N-starvation-responsive miRNAs and advances our understanding of the regulation of N homeostasis mediated by miRNAs. 相似文献
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Inhibition of micro-RNA-induced RNA silencing by 2′-O-methyl oligonucleotides in Drosophila S2 cells
Berger EM Dubrovsky EB Appleby L Dubrovskaya V 《In vitro cellular & developmental biology. Animal》2005,41(1-2):12-18
Summary More than 90 different micro-ribonucleic acid (miRNA) encoding genes have been identified in Drosophila, yet the function of only two of these, bantam and DmiR-14, has been elucidated. In an effort to develop a general strategy for the analysis of miRNA function in Drosophila, two procedures were developed, in a Schneider line 2 cell culture system, which may be adapted to that end. First, we show
that endogenous miRNAs can partially inhibit the expression of a transiently transfected reporter gene that has been modified
to contain sequences complementary to that miRNA in the 3′ UTR of a target messenger RNA (mRNA). Inhibition occurs by RNA
interference (RNAi), which involves mRNA degradation. Second, we demonstrate that this miRNA-induced RNAi can be partially
rescued with 2′-O-methyl oligonucleotides that contain sequences complementary to the cognate miRNA. We discuss how these techniques may be
used, in vivo, both for localizing the tissue distribution of endogenous miRNAs during Drosophila development and identifying phenotypes associated with a loss of miRNA function. 相似文献