共查询到20条相似文献,搜索用时 31 毫秒
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Harris LJ Saparno A Johnston A Prisic S Xu M Allard S Kathiresan A Ouellet T Peters RJ 《Plant molecular biology》2005,59(6):881-894
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Expression of the Cry2Aa2 protein was targeted specifically to the green tissues of transgenic tobacco Nicotiana tabacum cv. Xanthi plants. This deployment was achieved by using the promoter region of the gene encoding the Solanum tuberosum leaf and stem specific (ST-LS1) protein. The accumulated levels of toxin in the leaves were found to be effective in achieving 100 mortality of Heliothis virescens larvae. The levels of Cry2Aa2 expression in the leaves of these transgenic plants were up to 0.21 of the total soluble proteins. Bioassays with R1 transgenic plants indicated the inheritance of cry2Aa2 in the progeny plants. Tissue-specific expression of the Bt toxin in transgenic plants may help in controlling the potential occurrence of insect resistance by limiting the amount of toxin to only predated tissues. The results reported here validate the use of the ST-LS1 gene promoter for a targeted expression of Bt toxins in green tissues of plants. 相似文献
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Activity of a maize ubiquitin promoter in transgenic rice 总被引:27,自引:0,他引:27
Maria-Jesús Cornejo Diane Luth Kathleen M. Blankenship Olin D. Anderson Ann E. Blechl 《Plant molecular biology》1993,23(3):567-581
We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli. Histochemical localization of GUS activity revealed that UBI was most active in rapidly dividing cells. This promoter is expressed in many, but not all, rice tissues and undergoes important changes in activity during the development of transgenic rice plants. 相似文献
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The structure of the plant inflorescence and flower is an important agronomic and ornamental trait studied for its potential
economic applications. In particular, the capacity to modify flower size has always been a breeder’s goal. Genetic and molecular
studies have shown that the Zea mays gene Ramosa1 (Ra1) is involved in inflorescence branching regulation. In fact the ra1 loss of function mutation causes extra branching of the inflorescence. In this work we suggest a possible utilization of
the Ramosa1 maize gene as a tool to modify inflorescence architecture and flower size in transgenic plants. In fact overexpression of
this gene in Arabidopsis plants promotes an increase in reproductive organ size. Pollen, seeds, cotyledons, leaves and roots are also larger than
those of the wild type. Analysis of organs from transformants showed that cell expansion was increased without apparently
affecting cell division. These results suggest that the RA1 protein is able to up-regulate cell expansion in all organs of
Arabidopsis plants. 相似文献
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Intron-dependent transient expression of the maize GapA1 gene 总被引:2,自引:0,他引:2
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The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber
formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides
in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs. 相似文献
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Phytochelatins (PCs) are heavy metal binding peptides that play an important role in sequestration and detoxification of heavy
metals in plants. In this study, our goal was to develop transgenic plants with increased tolerance for and accumulation of
heavy metals from soil by expressing an Arabidopsis
thaliana
AtPCS1 gene, encoding phytochelatin synthase (PCS), in Indian mustard (Brassica juncea L.). A 35S promoter fused to a FLAG–tagged AtPCS1 cDNA was expressed in Indian mustard, and transgenic lines, designated pc lines, were evaluated for tolerance to and accumulation
of Cd and Zn. Transgenic plants with moderate AtPCS1 expression levels showed significantly higher tolerance to Cd and Zn
stress, but accumulated significantly less Cd and Zn than wild type plants in both shoot and root tissues. However, transgenic
plants with highest expression of the transgene did not exhibit enhanced Cd and Zn tolerance. Shoots of Cd-treated pc plants
had significantly higher levels of phytochelatins and thiols than wild-type plants. Significantly lower concentrations of
gluthatione in Cd-treated shoot and root tissues of transgenic plants were observed. Moderate expression levels of phytochelatin
synthase improved the ability of Indian mustard to tolerate certain levels of heavy metals, but at the same time did not increase
the accumulation potential for Cd and Zn. 相似文献
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