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1.
Jiang GL Dong Y Shi J Ward RW 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(8):1043-1052
Fusarium head blight (FHB or scab) caused by Fusarium species is a destructive disease in wheat, not only causing dramatic decrease of grain yield and quality, but also leading
to serious mycotoxin contamination in the infected grains. This study was conducted to identify and quantify quantitative
trait loci (QTLs) contributing to resistance to deoxynivalenol (DON) accumulation as well as to grain yield loss in a population
of 152 F7 recombinant inbred lines (RILs) derived from the cross Veery/CJ 9306. DON content in scabby grains and relative decreases
of yield components were analyzed. Two new QTLs (QFhs.nau-2DL and QFhs.nau-1AS) for resistance to DON accumulation caused by FHB in wheat were detected, and QTLs QFhs.ndsu-3BS and QFhs.nau-5AS were also validated in CJ 9306, based on a constructed genetic linkage map. On the average of three experiments, major QTLs
QFhs.ndsu-3BS and QFhs.nau-2DL explained up to 23 and 20% of phenotypic variation, respectively. QFhs.nau-1AS and QFhs.nau-5AS separately explained 4–6% of phenotypic variation. The differences among years/experiments were significant for all the four
QTLs. However, the QTL × environment interaction was significant only for QFhs.nau-2DL, but not for the others. The results suggest that simple sequence repeat (SSR) markers Xgwm533b associated with QFhs.ndsu-3BS, and Xgwm539 associated with QFhs.nau-2DL could be used in marker-assisted selection to enhance resistance to DON accumulation. QFhs.ndsu-3BS + QFhs.nau-2DL and QFhs.nau-2DL + QFhs.nau-5AS would be the optimum choices for two-locus combinations. QFhs.ndsu-3BS was also validated in CJ 9306 for resistance to grain yield loss, explaining 8–15% of phenotypic variation. No QTLs for resistance
to DON accumulation or grain yield loss independent of Type II resistance were found. By comparison, however, either of QFhs.nau-2DL or QFhs.nau-5AS alone and their combination were more contributive to resistance to DON accumulation than to Type II resistance. 相似文献
2.
Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat 总被引:19,自引:0,他引:19
Gervais L Dedryver F Morlais JY Bodusseau V Negre S Bilous M Groos C Trottet M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(6):961-970
Fusarium head blight (FHB) caused by Fusarium culmorum is an economically important disease of wheat that may cause serious yield and quality losses under favorable climate conditions. The development of disease-resistant cultivars is the most effective control strategy. Worldwide, there is heavy reliance on the resistance pool originating from Asian wheats, but excellent field resistance has also been observed among European winter wheats. The objective of this study was to map and characterize quantitative traits loci (QTL) of resistance to FHB among European winter wheats. A population of 194 recombinant inbred lines (RILs) was genotyped from a cross between two winter wheats Renan (resistant)/Récital (susceptible) with microsatellites, AFLP and RFLP markers. RILs were assessed under field conditions For 3 years in one location. Nine QTLs were detected, and together they explained 30-45% of the variance, depending on the year. Three of the QTLs were stable over the 3 years. One stable QTL, QFhs.inra.2b, was mapped to chromosome 2B and two QTLs QFhs.inra.5a2 and QFhs.inra5a3, to chromosome 5A; each of these QTLs explained 6.9-18.6% of the variance. Other QTLs were identified on chromosome 2A, 3A, 3B, 5D, and 6D, but these had a smaller effect on FHB resistance. One of the two QTLs on chromosome 5A was linked to gene B1 controlling the presence of awns. Overlapping QTLs for FHB resistance were those for plant height or/and flowering time. Our results confirm that wheat chromosomes 2A, 3A, 3B, and 5A carry FHB resistance genes, and new resistance factors were identified on chromosome arms 2BS and 5AL. Markers flanking these QTLs should be useful tools for combining the resistance to FHB of Asian and European wheats to increase the resistance level of cultivars. 相似文献
3.
Lin F Xue SL Zhang ZZ Zhang CQ Kong ZX Yao GQ Tian DG Zhu HL Li CJ Cao Y Wei JB Luo QY Ma ZQ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(3):528-535
Fusarium head blight (FHB) is a serious disease in wheat and barley affecting both yield and quality. To identify genes for
resistance to infection, the RIL population derived from ‘Nanda2419’ × ‘Wangshuibai’ and the parents were evaluated for percentage
of infected spikes (PIS) in four different environments. Using a 2,960 cM marker framework map constructed for this population,
ten chromosome regions were detected for their association with type I resistance through interval mapping with Mapmaker/QTL,
among which QTLs mapped in the intervals of Xwmc349~Xgwm149 on chromosome 4B, of Xwmc96~Xgwm304 on chromosome 5A and of Xgwm408~Xbarc140 on chromosome 5B were revealed in at least three environments and have Wangshuibai as the source of resistance alleles. Qfhi.nau-4B and Qfhi.nau-5A had larger effects and explained up to 17.5 and 27.0% of the phenotypic variance, respectively. To detect epistasis QTLs,
two-locus interactions were examined by whole genome scan. Interactions of five locus pairs were found to have significant
effects on type I resistance with the LOD score ranging 3.8–6.5 and four of them conferred resistance in parental phase. The
one with the most significant effect was Xcfd42~Xgwm469 (6D)/Xwmc390-2~Xbd04 (2A) pair. No QTL × E interaction was detected for PIS. It was found that flowering time did not have significant effects
on PIS in this population. Our studies indicated that Wangshuibai is useful for breeding for both type I and type II scab
resistance and the markers associated with the QTLs could be used in marker-assisted selection and isolation of scab-resistance
QTLs.
F. Lin and S.L. Xue equally contributed to this article 相似文献
4.
J. A. Anderson R. W. Stack S. Liu B. L. Waldron A. D. Fjeld C. Coyne B. Moreno-Sevilla J. Mitchell Fetch Q. J. Song P. B. Cregan R. C. Frohberg 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1164-1168
Genetic resistance to Fusarium head blight (FHB), caused by Fusarium graminearum, is necessary to reduce the wheat grain yield and quality losses caused by this disease. Development of resistant cultivars
has been slowed by poorly adapted and incomplete resistance sources and confounding environmental effects that make screening
of germplasm difficult. DNA markers for FHB resistance QTLs have been identified and may be used to speed the introgression
of resistance genes into adapted germplasm. This study was conducted to identify and map additional DNA markers linked to
genes controlling FHB resistance in two spring wheat recombinant inbred populations, both segregating for genes from the widely
used resistance source ’Sumai 3’. The first population was from the cross of Sumai 3/Stoa in which we previously identified
five resistance QTLs. The second population was from the cross of ND2603 (Sumai 3/Wheaton) (resistant)/ Butte 86 (moderately
susceptible). Both populations were evaluated for reaction to inoculation with F. graminearum in two greenhouse experiments. A combination of 521 RFLP, AFLP, and SSR markers were mapped in the Sumai 3/Stoa population
and all DNA markers associated with resistance were screened on the ND2603/Butte 86 population. Two new QTL on chromosomes
3AL and 6AS wer found in the ND2603/Butte 86 population, and AFLP and SSR markers were identified that explained a greater
portion of the phenotypic variation compared to the previous RFLP markers. Both of the Sumai 3-derived QTL regions (on chromosomes
3BS, and 6BS) from the Sumai 3/Stoa population were associated with FHB resistance in the ND2603/Butte 86 population. Markers
in the 3BS QTL region (Qfhs.ndsu-3BS) alone explain 41.6 and 24.8% of the resistance to FHB in the Sumai 3/Stoa and ND2603/Butte 86 populations, respectively.
This region contains a major QTL for resistance to FHB and should be useful in marker-assisted selection.
Received: 17 August 2000 / Accepted: 16 October 2000 相似文献
5.
Fusarium head blight (FHB) is one of the most devastating wheat diseases, causing both yield loss and quality reduction. To detect
quantitative trait loci (QTL) responsible for FHB resistance, plants of the F
2:3 population derived from a ‘Wangshui-bai’ × ‘Sy95-7’ cross were artificially inoculated. Of 396 simple sequence repeats (SSRs),
125 amplified fragment length polymorphisms were used for FHB resistance QTL analysis. Five QTLs for FHB resistance were detected
on chromosomes 3B, 6B, 7A, 1B and 2D. The effect of the QTL located on chromosome 3B on phenotypic variation was 31.69%, while
that of the QTL found on 2D was the smallest and only accounted for 4.98% of the variation. The resistance alleles originated
from ‘Wangshibai’ and association of the QTLs using these SSR markers may facilitate marker-assisted selection to improve
FHB resistance in the wheat breeding programs of southwest China. 相似文献
6.
QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (<Emphasis Type="Italic">Triticum aestivum</Emphasis> L.) 总被引:9,自引:0,他引:9
Paillard S Schnurbusch T Tiwari R Messmer M Winzeler M Keller B Schachermayr G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(2):323-332
Fusarium head blight (FHB) of wheat is a widespread and destructive disease which occurs in humid and semi-humid areas. FHB epidemics can cause serious yield and quality losses under favorable climatic conditions, but the major concern is the contamination of grains with mycotoxins. Resistance to FHB is quantitatively inherited and greatly influenced by the environment. Its evaluation is costly and time-consuming. The genetic basis of FHB resistance has mainly been studied in spring wheat. The objective of this study was to map quantitative trait loci (QTLs) for resistance to FHB in a population of 240 recombinant inbred lines (RILs) derived from a cross between the two Swiss winter wheat cultivars Arina (resistant) and Forno (susceptible). The RILs were genotyped with microsatellite and RFLP markers. The resulting genetic map comprises 380 loci and spans 3,086 cM. The 240 RILs were evaluated for resistance to FHB in six field trials over 3 years. Composite interval mapping (CIM) analyses carried out on FHB AUDPC (i.e. mean values across six environments) revealed eight QTLs which altogether explained 47% of the phenotypic variance. The three main QTLs were mapped on the long arms of chromosomes 6D (R2=22%), 5B (R2=14%) and 4A (R2=10%). The QTL detected on 5B originated from the susceptible parent Forno. Other QTLs with smaller effects on FHB resistance were detected on chromosomes 2AL, 3AL, 3BL, 3DS and 5AL.Communicated by H.C. Becker 相似文献
7.
Kazuhiro Sato Kiyosumi Hori Kazuyoshi Takeda 《Molecular breeding : new strategies in plant improvement》2008,22(4):517-526
Fusarium head blight (FHB) resistance was evaluated in five recombinant inbred (RI) populations. The RI populations consisted
of top-cross progeny derived from a diallel set of crosses. Each of five two-row barley lines differing in response to FHB
were crossed with ‘Harbin 2-row’. FHB severity was scored on an 11-point scale, where resistant = 0 and susceptible = 10,
based on the ‘cut-spike test’. Disease data were obtained for each population for 2 or 3 years. Linkage maps comprised of
expressed sequence tag (EST) markers were developed for each population and used for quantitative trait locus (QTL) detection.
Thirty two QTLs were detected using all data sets (individual populations and years). Thirteen QTLs were detected using averages
across years; 10 of these were consistent across the individual year and average data sets. These QTLs clustered at 14 regions,
with clusters on all chromosomes. At 11 of these clusters, Harbin 2-row contributed FHB resistance alleles. No QTLs were detected
near the row type (vrs1) locus in any of the five RI populations, suggesting that the FHB resistance QTL in this region reported in two-row × six-row
crosses may be pleiotropic effect of vrs1. QTL were coincident with the flowering type locus (cly1/Cly2) on chromosome 2H in every population. Some QTL × QTL interactions were significant, but these were smaller than QTL main
effects. Considering the pleiotropic effect of spike morphology on FHB resistance, future FHB resistance mapping efforts in
barley should focus on cross combinations in which alleles at vrs1 are not segregating.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
8.
Liu S Zhang X Pumphrey MO Stack RW Gill BS Anderson JA 《Functional & integrative genomics》2006,6(2):83-89
A major quantitative trait locus (QTL), Qfhs.ndsu-3BS, for resistance to Fusarium head blight (FHB) in wheat has been identified and verified by several research groups. The objectives
of this study were to construct a fine genetic map of this QTL region and to examine microcolinearity in the QTL region among
wheat, rice, and barley. Two simple sequence repeat (SSR) markers (Xgwm533 and Xgwm493) flanking this QTL were used to screen for recombinants in a population of 3,156 plants derived from a single F7 plant heterozygous for the Qfhs.ndsu-3BS region. A total of 382 recombinants were identified, and they were genotyped with two more SSR markers and eight sequence-tagged
site (STS) markers. A fine genetic map of the Qfhs.ndsu-3BS region was constructed and spanned 6.3 cM. Based on replicated evaluations of homozygous recombinant lines for Type II FHB
resistance, Qfhs.ndsu-3BS, redesignated as Fhb1, was placed into a 1.2-cM marker interval flanked by STS3B-189 and STS3B-206. Primers of STS markers were designed from wheat
expressed sequence tags homologous to each of six barley genes expected to be located near this QTL region. A comparison of
the wheat fine genetic map and physical maps of rice and barley revealed inversions and insertions/deletions. This suggests
a complex microcolinearity among wheat, rice, and barley in this QTL region. 相似文献
9.
A. Salameh M. Buerstmayr B. Steiner A. Neumayer M. Lemmens H. Buerstmayr 《Molecular breeding : new strategies in plant improvement》2011,28(4):485-494
Breeding for fusarium head blight (FHB) resistance of wheat is a continuous challenge for plant breeders. Resistance to FHB
is a quantitative trait, governed by several to many genes and modulated by environmental conditions. The presented study
was undertaken to assess the effect on improving FHB resistance and on possible unwanted side effects (‘linkage drag’) of
two resistance QTL, namely Fhb1 and Qfhs.ifa-5A, from the spring wheat line CM-82036 when transferred by marker-assisted backcrossing into several European winter wheat
lines. To achieve these goals, we developed and evaluated fifteen backcross-two–derived families based on nine European winter
wheat varieties as recipients and the FHB resistant variety CM-82036 as resistance donor. The QTL Qfhs.ifa-5A had a relatively small impact on increasing FHB resistance. On average lines with Fhb1 plus Qfhs.ifa-5A combined were only slightly more resistant compared to lines with Fhb1 alone. The obtained results suggest that the effect of the spring wheat–derived QTL on improving FHB resistance increases
in the order Qfhs.ifa-5A < Fhb1 ≤ Qfhs.ifa-5A plus Fhb1 combined. The genetic background of the recipient line had a large impact on the resistance level of the obtained lines.
No systematic negative effect of the spring wheat–derived QTL on grain yield, thousand grain weight, hectoliter weight and
protein content was found. The use of spring wheat–derived FHB resistance QTL for breeding high yielding cultivars with improved
FHB resistance appears therefore highly promising. 相似文献
10.
Zhang G Mergoum M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(6):757-766
Kernel shattering (KS) can cause severe grain yield loss in wheat (Triticum aestivum L.). The introduction of genotypes with Fusarium head blight (FHB) resistance has elevated the KS importance. ‘Sumai3,’ the
most commonly used FHB-resistant germplasm worldwide, is reported to be KS susceptible. The objectives of this study were
to detect quantitative trait loci (QTLs) for KS and to determine the relationship between KS and FHB. A recombinant inbred
line population derived from a cross between Sumai3 and ‘Stoa’ was evaluated for KS in five environments and FHB in two field
trials, separately. Four genomic regions on chromosomes 2B, 3B, and 7A were associated with KS. Of them, two major KS QTLs
were detected consistently over three environments and each located proximal to the centromere on chromosomes 3B and 7A. The
resistant alleles at these two QTLs together can reduce KS by 66.1% relative to the reciprocal alleles and by 41.1% compared
to the population mean. The field FHB data revealed four QTLs on chromosomes 2B, 3B, and 7A. Three of these FHB QTLs coincided
with and/or linked to the KS QTLs with opposite allele effects in the corresponding genomic regions, which may explain the
negative correlation (r = −0.29 and P < 0.01) between the KS and FHB infection found in this study. The results in this study indicate that KS and FHB in Sumai3
are, in part, inherited dependently. However, the correlation between KS and FHB is not strong, and the major FHB resistance
QTL on chromosome arm 3BS was not linked to any KS QTL. Our results showed that pyramiding of the two major KS-resistant alleles
and the unlinked major FHB-resistant allele could produce lines with both low values of KS and FHB infection. 相似文献
11.
Schnurbusch T Paillard S Fossati D Messmer M Schachermayr G Winzeler M Keller B 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(7):1226-1234
Stagonospora nodorum is the causal agent of the Stagonospora glume blotch disease in hexaploid wheat. The Swiss winter bread wheat cv. 'Arina' has a highly effective, durable and quantitative glume blotch resistance. We studied 240 single seed descent (SSD)-derived lines of an 'Arina × Forno' F5:7 population to identify and map quantitative trait loci (QTLs) for glume blotch resistance under natural infestation. Using composite interval mapping (CIM) and LOD>4.5, we detected two chromosomal regions on chromosome arms 3BS and 4BL which were specifically associated with glume blotch resistance. These identified QTLs were designated QSng.sfr-3BS and QSng.sfr-4BL, respectively. QSng.sfr-3BS peaked at the locus Xgwm389 in the telomeric region of the short arm of chromosome 3B and explained 31.2% of the observed phenotypic variance for the resistance within the population. The responsible QSng.sfr-3BS allele originated from the resistant parent 'Arina'. The QTL QSng.sfr-4BL (19.1%) mapped to chromosome arm 4BL ('Forno' allele) very close to two known genes, TaMlo and a catalase (Cat). Both QTL alleles combined could enhance the resistance level by about 50%. Additionally, they showed significant epistatic effects (4.4%). We found PCR-based microsatellite markers closely linked to QSng.sfr-3BS (gwm389) and QSng.sfr-4BL (gwm251) which make marker-assisted selection (MAS) for Stagonospora glume blotch resistance feasible. We also found one resistance QTL, QSng.sfr-5BL, on the long arm of chromosome 5B which overlapped with QTLs for plant height as well as heading time.Communicated by H. C. Becker 相似文献
12.
Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina 总被引:3,自引:0,他引:3
Draeger R Gosman N Steed A Chandler E Thomsett M Srinivasachary Schondelmaier J Buerstmayr H Lemmens M Schmolke M Mesterhazy A Nicholson P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(5):617-625
Fusarium head blight (FHB) of wheat has become a serious threat to wheat crops in numerous countries. In addition to loss
of yield and quality, this disease is of primary importance because of the contamination of grain with mycotoxins such as
deoxynivalenol (DON). The Swiss winter cultivar Arina possesses significant resistance to FHB. The objective of this study
was to map quantitative trait loci (QTL) for resistance to FHB, DON accumulation and associated traits in grain in a double
haploid (DH) population from a cross between Arina and the FHB susceptible UK variety Riband. FHB resistance was assessed
in five trials across different years and locations. Ten QTL for resistance to FHB or associated traits were detected across
the trials, with QTL derived from both parents. Very few of the QTL detected in this study were coincident with those reported
by authors of two other studies of FHB resistance in Arina. It is concluded that the FHB resistance of Arina, like that of
the other European winter wheat varieties studied to date, is conferred by several genes of moderate effect making it difficult
to exploit in marker-assisted selection breeding programmes. The most significant and stable QTL for FHB resistance was on
chromosome 4D and co-localised with the Rht–D1 locus for height. This association appears to be due to linkage of deleterious genes to the Rht-D1b (Rht2) semi-dwarfing allele rather than differences in height per se. This association may compromise efforts to enhance FHB resistance
in breeding programmes using germplasm containing this allele. 相似文献
13.
Schmolke M Zimmermann G Buerstmayr H Schweizer G Miedaner T Korzun V Ebmeyer E Hartl L 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(4):747-756
Fusarium head blight (FHB), mainly caused by Fusarium graminearum and F. culmorum, can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. The objective of this study was to identify quantitative trait loci (QTLs) for FHB resistance in a winter wheat population developed by crossing the resistant German cultivar Dream with the susceptible British cultivar Lynx. A total of 145 recombinant inbred lines (RILs) were evaluated following spray inoculation with a F. culmorum suspension in field trials in 2002 in four environments across Germany. Based on amplified fragment length polymorphism and simple sequence repeat marker data, a 1,734 cM linkage map was established assuming that the majority of the polymorphic parts of the genome were covered. The area under disease progress curve (AUDPC) was calculated based on the visually scored FHB symptoms. The population segregated quantitatively for FHB severity. Composite interval mapping analysis for means across the environments identified four FHB resistance QTLs on chromosomes 6AL, 1B, 2BL and 7BS. Individually the QTLs explained 19%, 12%, 11% and 21% of the phenotypic variance, respectively, and together accounted for 41%. The QTL alleles conferring resistance on 6AL, 2BL and 7BS originated from cv. Dream. The resistance QTL on chromosome 6AL partly overlapped with a QTL for plant height. The FHB resistance QTL on 7BS coincided with a QTL for heading date, but the additive effect on heading date was of minor importance. The resistance QTL on chromosome 1B was associated with the T1BL.1RS wheat-rye translocation of Lynx. 相似文献
14.
15.
Apotikar DB Venkateswarlu D Ghorade RB Wadaskar RM Patil JV Kulwal PL 《Journal of genetics》2011,90(1):59-66
Sorghum (Sorghum bicolor (L.) Moench) is one of the most important crops in the semiarid regions of the world. One of the important biotic constraints
to sorghum production in India is the shoot fly which attacks sorghum at the seedling stage. Identification of the genomic
regions containing quantitative trait loci (QTLs) for resistance to shoot fly and the linked markers can facilitate sorghum
improvement programmes through marker-assisted selection. A simple sequence repeat (SSR) marker- based skeleton linkage map
of two linkage groups of sorghum was constructed in a population of 135 recombinant inbred lines (RIL) derived from a cross
between IS18551 (resistant to shoot fly) and 296B (susceptible to shoot fly). A total of 14 SSR markers, seven each on linkage
groups A and C were mapped. Using data of different shoot fly resistance component traits, one QTL which is common for glossiness,
oviposition and dead hearts was detected following composite interval mapping (CIM) on linkage group A. The phenotypic variation
explained by this QTL ranged from 3.8%–6.3%. Besides the QTL detected by CIM, two more QTLs were detected following multi-trait
composite interval mapping (MCIM), one each on linkage groups A and C for the combinations of traits which were correlated
with each other. Results of the present study are novel as we could find out the QTLs governing more than one trait (pleiotropic
QTLs). The identification of pleiotropic QTLs will help in improvement of more than one trait at a time with the help of the
same linked markers. For all the QTLs, the resistant parent IS18551 contributed resistant alleles. 相似文献
16.
H. Zhu L. Gilchrist P. Hayes A. Kleinhofs D. Kudrna Z. Liu L. Prom B. Steffenson T. Toojinda H. Vivar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(7-8):1221-1232
Fusarium head blight (FHB), an important disease of barley in many areas of the world, causes losses in grain yield and quality. Deoxynivalenol
(DON) mycotoxin residues, produced by the primary pathogen Fusarium graminearum, pose potential health risks. Barley producers may not be able to profitably market FHB-infected barley, even though it has
a low DON level. Three types of FHB resistance have been described in wheat: Type I (penetration), Type II (spread), and Type
III (mycotoxin degradation). We describe putative measures of these three types of resistance in barley. In wheat, the three
resistance mechanisms show quantitative inheritance. Accordingly, to study FHB resistance in barley, we used quantitative
trait locus (QTL) mapping to determine the number, genome location, and effects of QTLs associated with Type-I and -II resistance
and the concentration of DON in the grain. We also mapped QTLs for plant height, heading date, and morphological attributes
of the inflorescence (seeds per inflorescence, inflorescence density, and lateral floret size). QTL analyses were based on
a mapping population of F1-derived doubled-haploid (DH) lines from the cross of the two-rowed genotypes Gobernadora and CMB643, a linkage map constructed
with RFLP marker loci, and field evaluations of the three types of FHB resistance performed in China, Mexico, and two environments
in North Dakota, USA. Resistance QTLs were detected in six of the seven linkage groups. Alternate favorable alleles were found
at the same loci when different inoculation techniques were used to measure Type-I resistance. The largest-effect resistance
QTL (for Type-II resistance) was mapped in the centromeric region of chromosome 2. All but two of the resistance QTLs coincided
with QTLs determining morphological attributes of the inflorescence and/or plant height. Additional experiments are needed
to determine if these coincident QTLs are due to linkage or pleiotropy and to more clearly define the biological basis of
the FHB resistance QTLs. Plant architecture should be considered in FHB resistance breeding efforts, particularly those directed
at resistance QTL introgression and/or pyramiding.
Received: 22 November 1998 / Accepted: 2 June 1999 相似文献
17.
Maccaferri M Ratti C Rubies-Autonell C Vallega V Demontis A Stefanelli S Tuberosa R Sanguineti MC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):527-544
Soil-borne cereal mosaic (SBCM) is a viral disease, which seriously affects hexaploid as well as tetraploid wheat crops in Europe. In durum wheat
(Triticum durum Desf.), the elite germplasm is characterized by a wide range of responses to SBCMV, from susceptibility to almost complete
resistance. In this study, the genetic analysis of SBCMV resistance was carried out using a population of 181 durum wheat
recombinant inbred lines (RILs) obtained from Meridiano (resistant) × Claudio (moderately susceptible), which were profiled
with SSR and DArT markers. The RILs were characterized for SBCMV response in the field under severe and uniform SBCMV infection
during 2007 and 2008. A wide range of disease reactions (as estimated by symptom severity and DAS-ELISA) was observed. A large
portion of the variability for SBCMV response was explained by a major QTL (QSbm.ubo-2BS) located in the distal telomeric region of chromosome 2BS near the marker triplet Xbarc35–Xwmc661–Xgwm210, with R
2 values ranging from 51.6 to 91.6%. The favorable allele was contributed by Meridiano. Several QTLs with minor effects on
SBCMV response were also detected. Consistently with the observed transgressive segregation, the resistance alleles at minor
QTLs were contributed by both parents. The presence and effects of QSbm.ubo-2BS were validated through association mapping in a panel of 111 elite durum wheat accessions. 相似文献
18.
Thomas Miedaner Peter Risser Sophie Paillard Thorsten Schnurbusch Beat Keller Lorenz Hartl Josef Holzapfel Viktor Korzun Erhard Ebmeyer H. Friedrich Utz 《Molecular breeding : new strategies in plant improvement》2012,29(3):731-742
Septoria tritici blotch (STB), caused by S. tritici, Stagonospora glume blotch (SGB), caused by S. nodorum, and Fusarium head blight (FHB), caused by F. graminearum and F. culmorum, are the most important diseases of wheat (Triticum aestivum L.) in temperate growing areas. The main goals of this study were to detect (1) new quantitative trait loci (QTL) for STB
resistance in two adapted European biparental populations (Arina/Forno, History/Rubens) and (2) QTL regions for broad-spectrum
resistance (BSR) to the above-mentioned diseases during the adult-plant stage in the field. The three resistances were phenotyped
across 4–7 field environments and phenotypic data revealed significant (P < 0.01) genotypic differentiation in all cases. Entry-mean heritabilities (h2) ranged from 0.73 to 0.93. For STB resistance, correlations between disease ratings and heading date were significant (P < 0.01), but moderate (r = −0.23 to −0.30) in both populations. Correlations between STB and plant height were higher in Arina/Forno (r = −0.45) and History/Rubens (r = −0.55), the latter population segregating at the Rht-D1 locus. During the initial QTL analysis, 5 QTL were detected for STB resistance in each of the populations, amounting to an
explained genotypic variance of 45–63%, thus, showing the same ranges as FHB and SGB resistances in Arina/Forno and FHB resistance
in History/Rubens. In total, 7 BSR QTL were found in the meta-analysis with the raw data, including the QTL on chromosome
4D at the Rht-D1 locus. A BSR QTL for all three diseases was not found but several BSR QTL for combinations with two diseases were detected.
Combining the BSR QTL detected in the present breeding material by applying marker-assisted selection seems a promising approach. 相似文献
19.
Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat 总被引:2,自引:0,他引:2
Xunfen Chen Justin D. Faris Jinguo Hu Robert W. Stack Tika Adhikari Elias M. Elias Shahryar F. Kianian Xiwen Cai 《Molecular breeding : new strategies in plant improvement》2007,19(2):113-124
Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified
in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T.
dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region
amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular
marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance
of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to
3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning
of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection. 相似文献
20.
Marker-assisted development and evaluation of near-isogenic lines for scab resistance QTLs of wheat 总被引:1,自引:0,他引:1
Shulin Xue Guoqiang Li Haiyan Jia Feng Lin Yong Cao Feng Xu Mingzhi Tang Yao Wang Xinyi Wu Zhengzhi Zhang Lixia Zhang Zhongxin Kong Zhengqiang Ma 《Molecular breeding : new strategies in plant improvement》2010,25(3):397-405
Fusarium head blight or scab resistance in wheat is a complex quantitative trait affected greatly by environments. Therefore, the quantitative trait loci (QTL) for scab resistance found in mapping projects require validation to be effectively utilized in breeding programs. In this study, by employing both forward and background selections with the help of molecular markers, near-isogenic lines (NILs) for scab resistance QTLs Qfh.nau-2B, Qfhs.nau-3B, Qfhi.nau-4B and Qfhi.nau-5A, three of which originating in scab resistance germplasm Wangshuibai, were developed with the elite line Miangyang 99-323 as the recurrent parent. During the process of backcross, selection was based solely on marker genotypes of the target regions, and on recipient genome recovery rate in BC2F1 and BC3F1. All the identified BC3F1 plants with the target QTL regions have more than 94% recipient genome composition (RGC), and out of four to five of them a plant with over 97% RGC were obtained in each backcross combination. Compared with Mianyang 99-323, the Qfhs.nau-3B NIL showed much better resistance to disease spread within spikes, the Qfhi.nau-4B and Qfhi.nau-5A NILs showed much better resistance to initial infection, and the Qfh.nau-2B NIL showed improvement in both types of resistance. These results were consistent with findings in the previous QTL mapping studies. Morphologically and agronomically these NILs were similar to Mianyang 99-323 except that Qfhi.nau-4B NIL was taller and had a longer spike, and Qfhi.nau-5A NIL had narrower leaves. These results demonstrated the feasibility of marker-assisted utilization of scab resistance QTLs. 相似文献