调控胚胎干细胞生长的转录因子

胚胎干细胞的生长是由一个极其复杂的网络系统调控的,本文简要叙述了Oct-4、Nanog、Sox2三个转录因子对胚胎干细胞生长的调控作用,为将来更好的开发利用胚胎干细胞资源奠定理论基础.     

LncKdm2b controls self‐renewal of embryonic stem cells via activating expression of transcription factor Zbtb3

Divergent long noncoding RNAs (lncRNAs) represent a major lncRNA biotype in mouse and human genomes. The biological and molecular functions of the divergent lncRNAs remain largely unknown. Here, we show that lncKdm2b, a divergent lncRNA for Kdm2b gene, is conserved among five mammalian species and highly expressed in embryonic stem cells (ESCs) and early embryos. LncKdm2b knockout impairs ESC self‐renewal and causes early embryonic lethality. LncKdm2b can activate Zbtb3 by promoting the assembly and ATPase activity of Snf2‐related CREBBP activator protein (SRCAP) complex in trans. Zbtb3 potentiates the ESC self‐renewal in a Nanog‐dependent manner. Finally, Zbtb3 deficiency impairs the ESC self‐renewal and early embryonic development. Therefore, our findings reveal that lncRNAs may represent an additional layer of the regulation of ESC self‐renewal and early embryogenesis.     

转录因子Sox2的研究进展

转录因子Sox2是sox基因家族的一个成员,由于它在早期胚胎发生、神经分化和晶状体发育等多种重要的发育事件中都起着关键的作用,从而引起了越来越广泛的关注。本文就小鼠sox2基因的研究进展作一综述。     

Sox2 roles in neural stem cells

Throughout vertebrate evolution, Sox2 marks the developing nervous system from its earliest developmental stages and, therein, the most undifferentiated precursor cells, including stem cells. Recent gene targeting studies investigated the function of Sox2 in two neuronal systems: the developing eye and brain. These studies uncovered a requirement for Sox2 in the maintenance of neural stem cells, as well as a downstream role in the differentiation of specific neuron sub-types. In both systems, Sox2 action is markedly dose-dependent, and downstream-target gene studies are beginning to reveal the mechanisms of Sox2 function.