首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 0 毫秒
1.
一种含有单链RNA的香菇球状病毒   总被引:6,自引:0,他引:6  
从生长不正常的香菇(Lentinus edodes(Berk.)Sing)菌株中分离到一种等轴对称含单链RNA的病毒颗粒。病毒颗粒在电镜下直径为33~34nm,在SDS-聚丙烯酰胺凝胶电泳中病毒外壳蛋白分子量为22000道尔顿。病毒核酸径DNase1和SI酶解试验及热变性紫外吸收曲线试验证明为单链RNA,在1.5%的琼脂糖凝胶电泳中,病毒核酸呈现一条带,分子量为2.38×10~6道尔顿。  相似文献   

2.
香菇栽培料新工艺   总被引:4,自引:0,他引:4  
在国内香菇栽培料常规配方基础上,设计了添加2.5mL盐酸/kg取代常规配方中1%蔗糖的新工艺,经测定,2种栽培料灭菌后还原糖与蔗糖浓度、发菌期与子实体产量基本一致,新配方栽培料灭菌后pH值为5.6,是香菇菌丝生长最适pH值。新配方工艺可降低生产成本10%左右。  相似文献   

3.
The present paper reports for the first time the transformation of an organic selenium compound into red selenium (Se), which causes the intense red pigmentation of Lentinula edodes (shiitake mushroom) mycelia. The biotransformation of 1,5-diphenyl-3-selenopentanedione-1,5 (diacetophenonyl selenide, preparation DAPS-25) was studied in liquid- and solid-phase cultures of L. edodes. In liquid culture medium, a red color develops in the mycelium at initial DAPS-25 concentrations equal to or higher than 0.1?mmol/l. The intensity and initiation time of coloration is Se concentration-dependent. Semiquantitative data obtained by physicochemical methods on the extent of Se and acetophenone production suggest that L. edodes is able to absorb and/or destruct this organic Se xenobiotic.  相似文献   

4.
PEG介导下香菇的转化   总被引:8,自引:0,他引:8  
表达载体p301-bG1含有香菇(Lentinus edodes (Berk.)Sing)三磷酸甘油醛脱氢酶启动子驱动下的gus基因和除草剂抗性基因。利用PEG法实现了p301-bG1对香菇原生质体的转化。香菇原生质体与经PEG纯化的质粒DNA混合,用PEG处理后培养于含40ug/mL除草剂的CYM再生平板上,得到了抗除草剂和有GUS活性的转化菌株。虽然这种方法转化效率较低,但不需要昂贵的仪器和限制性内切酶,为蘑菇的分子育种研究提供了一种简便经济的转化方法。  相似文献   

5.
The hemagglutinating activity of submerged mycelium and culture liquid for four strains of Lentinus edodes (Berk.) Sing [L. edodes (Berk.) Pegler] was studied in the search for lectins. The hemagglutinating activity of culture liquid was substantially higher, compared with mycelium. The carbohydrate-binding capacity of the agglutinins was established, and the lectin activity of extracts from mycelia grown on several agar media was elucidated in relation to fruiting. The lectin activity of L. edodes was examined at different morphogenetic steps: mycelium, brown mycelial film, primordium, and fruiting body. Hemagglutination titers at the brown film step were higher than in the mycelium, whereas activity at the primordial and fruiting bodies steps decreased. Lectins seem to be involved in the formation of hyphal aggregates of brown mycelial film.  相似文献   

6.
One of the chitinases secreted in the culture filtrate of a gram-negative bacteria, Burkholderia cepacia strain KH2, which was isolated from the bed log of Lentinus edodes, Shiitake mushrooms, was purified by DEAE Sepharose CL-6B chromatography, followed by Sephacryl S-100 HR gel filtration. The purified enzyme was homogenous, determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with an estimated molecular weight of 34,000 and an isoelectric point (pI) of 5.9. The enzyme was stable at pH values of 4.0-6.0, and at temperatures up to 50 degrees C; the optimum pH and temperature were 4.5 and 50 degrees C, respectively. The enzyme exhibited higher activities toward chitosan 7B, a 62% deacetylated chitosan, than toward the highly deacetylated chitosan substrates. The enzyme was observed to drastically hydrolyze partially deacetylated chitin substrates, with the subsequent formation of N-acetylchitooligosaccharides [(GlcNAc) (n), n=2-7]. Separation and quantification of the hydrolysis products of (GlcNAc) (n), n52-6, by HPLC showed the splitting into (GlcNAc)(n), n=3-6. Activity toward N-acetylchitobiose was not detected. Oligomers with a higher number of units than the starting substrate were also detected, which indicate transglycosylation activity.  相似文献   

7.
Summary Tannin at 1% (w/v) did not inhibit the growth ofLentinus edodes, but did inhibitPleuroius florida, P. sajor-caju, P. cystidosus, Agaricus bisporus andVolvariella volvacea. The inhibition was not due to its acidity.
Tolérance de Lentinus edodes aux ajouts de tannin
Résumé Le tannin à la concentration de 1% (p/v) n'inhibe pas la croissance deLentinus edodes, mais inhibe celle dePleurotus florida, P. sajor-caju, P. cystidosus, Agaricus bisporus, etVolvariella volvacea. L'inhibition n'est pas due à son acidité.
  相似文献   

8.
Polyisoprenoid alcohols from the mushroom Lentinus edodes   总被引:2,自引:0,他引:2  
Lipids extracted from the shiitake mushroom Lentinus edodes contain dolichols composed of 15 up to 19 isoprene units with Dol-17 as the dominating prenologue. Identification of dolichols was achieved by the application of 2D-TLC, HPLC and electrospray ionization mass spectrometry. Additionally a family of polyprenols (-unsaturated counterparts) with the same chain-length was also detected. Dolichols comprised approximately 0.002% of the fresh weight of the mushroom. Dolichols accompanied by traces of polyprenols are for the first time found in the mushroom tissue.  相似文献   

9.
Isometric virus-like particles (VLPs) with diameter of approximately 34 nm containing ss-RNA were purified from abnormal mycelium of Shiitake mushroom, Lentinus edodes (Berk.) Sing. SDS-polyacrylamide gel elec-trophoresis demonstrated that the virions contain a single capsid polypeptide with molecular weight of about 22 000 daltons. The nucleic acid extract from purified VLPs preparations showed only one band with a size of approximately 7.3 kilobases. The susceptibility to RNase 1 and S1 digestions, resistance to DNase and thermal denaturation behaviour of the viral genome indicated that it is a single-stranded RNA. To our knowledge, isometric single-stranded RNA VLPs isolated from Shiitake mushroom mycelia have not been reported before.  相似文献   

10.
11.
12.
Substrate specificity of laccase from Lentinus edodes   总被引:1,自引:0,他引:1  
In previous studies, the white-rot basidiomycete Lentinus edodes, strain SC-495, was proved to be a “selective” lignin degrader and its extracellular crude preparations arising from solid-state cultures were successfully employed in biopulping experiments on annual plants. This fungus produced extracellular laccase as the predominant phenoloxidase when growing in solid-state fermentation on corn stalks. Laccase from this strain was purified and partially characterized, as an initial approach towards the study of its ligninolytic complex. Laccase was purified 69.6-fold by anion-exchange chromatography and two affinity-chromatography steps with an overall yield of 7.45%. The native enzyme exhibited a molecular mass of 74 kDa, an isoelectric point of 3.42 and a carbohydrate content of 7.5%. The absorption spectrum of laccase showed a maximum at 605 nm, typical of blue-copper oxidases. The optimum pH and temperature for the activity of laccase were 4.0–4.2 and 50°C, respectively. Kinetic experiments, performed with a wide range of phenolic compounds, showed that the reaction rate and the substrate affinity greatly varied depending on the nature of substituents and their reciprocal positions on the aromatic ring. In particular, the enzyme showed high affinity to phenolic compounds bearing methoxyl or methyl groups, but no affinity to those bearing the nitro group directly attached to the benzene ring, nor to non-phenolic lignin-related compounds, such as trans-cinnamic acid or 3,4-dimethoxycinnamic acid. The huge differences in terms of reactivity of the enzyme towards phenolic compounds suggests that a preliminary systematic screening should be advisable when using laccase in effluent treatment applications.  相似文献   

13.
香菇双单杂交后代不同发育阶段酯酶同工酶研究   总被引:1,自引:0,他引:1  
选用香菇野生株分别与栽培株及杂交株进行双单杂交 ,得到 8个杂交后代 ,且具有结实能力 ,分别对液培 2 0d杂交后代菌丝与液培原基酯酶同工酶进行了比较研究。结果表明 ,液培 2 0d菌丝菌株间酯酶同工酶酶谱显示出多样性 ,可以作为鉴定菌株的辅助遗传标记 ,而原基菌株间酯酶同工酶酶谱谱带较少 ,呈现趋同效应 ,不宜作为鉴定香菇菌株的辅助遗传标记。  相似文献   

14.
Salt-Regulated Mannitol Metabolism in Algae   总被引:1,自引:0,他引:1  
Mannitol, one of the most widely occurring sugar alcohol compounds, is found in bacteria, fungi, algae, and plants. In these organisms the compound acts as a compatible solute and has multiple functions, including osmoregulation, storage, and regeneration of reducing power, and scavenging of active oxygen species. Because of the diverse functions of mannitol, introducing the ability to accumulate it has been a hallmark of attempts to generate highly salt-tolerant transgenic plants. However, transgenic plants have not yet improved significantly in their salt tolerance. Recently, we purified and characterized 2 enzymes that biosynthesize mannitol, mannitol-1-phosphate dehydrogenase (M1PDH) and mannitol-1-phosphate-specific phosphatase, from the marine red alga Caloglossa continua, which grows in estuarine areas where tide levels fluctuate frequently. The activation of Caloglossa M1PDH is unique in that it is regulated by salt concentration at enzyme level. In this review we focus on the metabolism of mannitol, mainly in marine photosynthetic organisms, and suggest how this might be applied to producing salt-tolerant transgenic plants.  相似文献   

15.
A fruiting-body-specific mfbA cDNA derived from Lentinus edodes FMC2 has been shown to encode a high-molecular-weight protein, MFBA, containing the cell-adhesion-promoting Arg-Gly-Asp (RGD) sequence. Southern-blot analysis showed that all L. edodes strains tested have the mfbA gene (homologue). Nucleotide sequence analysis of the 1-kb mfbA fragments containing the RGD-coding sequence showed that each L. edodes strain has two types of mfbA homologues. It was found in FMC2 that two mfbA homologues are derived from different nuclei and these mfbA alleles are transcribed with similar frequencies in the fruiting bodies.  相似文献   

16.
本实验建立了HPLC法同时测定香菇中酒石酸、苹果酸、乙酸和柠檬酸的方法。在酸提条件下,利用Agela Venusll MP C18色谱柱,以2%CH3OH-20 mM(NH4)2HPO4(pH 2.40)为流动相,流速为1.0 mL/min,柱温为35℃,检测波长为210 nm,进样量为10μL。四种有机酸均在8 min内出峰。本法准确、可靠、快速、简便,检出限较低,适合于大批量样品的分析与检测。  相似文献   

17.
Three kinds of nuclease preparations, each of which having both endonuclease activity that formed 5′-mononucleotides and 3′-nucleotidase activity, were separated and partially purified from Shii-take, Lentinus edodes. Both enzyme activities of each preparation showed a similar thermostability and electrophoretic mobility on Polyacrylamide gel, and a competitive relationship was observed between RNA and 3′-AMP in their enzyme reactions. From these results, it is concluded that both enzyme activities of these three preparations reside in a single protein, respectively. They resemble one another in substrate specificity, cleavage pattern of RNA and thermostability, but are distinguishable from one another by molecular weight, electrophoretic mobility and optimum pH for degradation of RNA.  相似文献   

18.
Summary Lentinus edodes (Berk.) Sing. is a commercially important edible mushroom cultivated on oak (Quercus spp.) logs or wood particles. Inoculum (spawn) of this white-rot saprophyte, made by homogenizing cultures grown on a chemically defined liquid medium, dies when transferred to oak wood. This study shows that an aqueous extract of the oak wood is inhibitory to growth. Inoculum produced by homogenizing stationary-grown cultures was particularly sensitive to the extract. Three different methods decreased the sensitivity of inoculum: (1) use of intact (nonhomogenized) cultures as inoculum, (2) use of homogenized cultures produced from shaken rather than stationary cultures as inoculum, or (3) use of cultures grown in the presence of wood extract as inoculum. With the use of shaken cultures adapted to oak wood extract, homogenized-culture inoculum successfully colonized an oak wood medium. Adaptation of the fungus to extracts made from an intended substrate may be an inexpensive way of producing liquid spawn specifically suited for the inoculation of the substrate.This article was written and prepared by U.S. Government employees on official time, and it is therefore in the public domain (i.e., it cannot be copyrighted).Research carried out while T. J. Griffin was a volunteer worker at Forest Products Laboratory  相似文献   

19.
A Streptomyces-pepsin inhibitor (S-PI or Pepstatin Ac), and DAN-insensitive carboxyl proteinase was found in a still culture filtrate of Lentinus edodes. The new carboxyl proteinase was purified, and about 9 mg purified enzyme was obtained from 19 liters of culture filtrate, with 11% recovery. The enzyme showed a single band on polyacrylamide gel electrophoresis. The molecular weight and isoelectric point were 40,000 and pH 4.2, respectively. The enzyme did not contain histidine and was composed of 387 amino acid residues. The enzyme was most active between pH 2.7 ~ 2.9, and stable over a pH of 3.2 ~ 5.2 for 3 hr at 37°C. The enzyme was not inhibited by S-PI or synthetic pepsin inhibitors such as DAN and EPNP. The physicochemical and enzymological properties were very similar to those of Scytalidium lignicolum carboxyl proteinase A, which was reported to be an S-PI-, and DAN-insensitive carboxyl proteinase.  相似文献   

20.
Toyama H  Toyama N 《Microbios》2000,101(399):73-80
A mycelial mat of Lentinus edodes was treated with 0.01% (w/v) colchicine solution for 240 h at 26 degrees C and autopolyploidization occurred. The mycelia were treated subsequently with the haploidizing reagent, benomyl, and fanshaped sectors were produced from colonies. Among such sectors, cellulase hyperproducers could be selected. The cellulase productivity of the hyperproducer, L1, did not decrease through five generations.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号