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1.
AFLP-derived STS markers for the identification of sex in Asparagus officinalis L. 总被引:13,自引:0,他引:13
S. M. Reamon-Büttner C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):432-438
For a simple, rapid and PCR-based screening of sex in the cultivated asparagus (Asparagus officinalis L.), we developed five STS markers from previously mapped, low-copy, sex-linked AFLP markers. A male/female PCR assay was
feasible with these STS markers either by direct amplification or by digestion with restriction enzymes. Similar to the AFLP
markers from which they were derived, STS4150.1, STS4150.2, STS4150.3 and STS3156 did not give recombinants in five different
populations. STS3660 could be scored codominantly, enabling the differentiation of XY from YY males in the screened F2 mapping population. The use of the sex-linked STS markers should allow early identification of sex, thus accelerating the
breeding process for new asparagus varieties. Further, 10 additional AFLP markers obtained with PstI/MseI primer combinations have been mapped on the L5 chromosome, bringing the total number of known AFLP and STS markers flanking
the sex locus to 24. These markers can be utilized for fine mapping of the sex gene in asparagus, which will pave the way
for a map-based cloning approach.
Received: 31 May 1999 / Accepted: 22 June 1999 相似文献
2.
George ML Regalado E Li W Cao M Dahlan M Pabendon M Warburton ML Xianchun X Hoisington D 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(1):80-91
We have developed and evaluated sequence-tagged site (STS) primers based on expressed sequence-tag information derived from sugi (Cryptomeria japonica) for use in hinoki (Chamaecyparis obtusa), a species that belongs to a different family (although it appears to be fairly closely related to sugi). Of the 417 C. japonica STS primer pairs we screened, 120 (~30%) were transferable and provided specific PCR amplification products from 16 C. obtusa plus trees. We used haploid megagametophytes to investigate the homology of 80 STS fragments between C. obtusa and C. japonica and to identify orthologous loci. Nearly 90% of the fragments showed high (>70%) degrees of similarity between the species, and 35 STSs indicated homology to entries with the same putative function in a public DNA database. Of the 120 STS fragments amplified, 72 showed restriction fragment length polymorphisms; in addition, the CC2430 primers detected amplicon length polymorphism. We assessed the inheritance pattern of 27 cleaved amplified polymorphic sequence markers, using 20 individuals from the segregation population. All the markers analyzed were consistent with the marker inheritance patterns obtained from the screening panel, and no markers (except CC2716) showed significant (P<0.01) deviation from the expected segregation ratio. In total, 136 polymorphic markers were developed using C. japonica-based STS primers without any sequence modification. In addition, the applicability of STS-based markers developed in one species to other species was found to closely reflect the evolutionary distance between the species, which is roughly concordant with the difference between their rbcL sequences. We plan to use these markers for genetic studies in C. obtusa. Most of the markers should also provide reliable anchor loci for comparative mapping studies of the C. obtusa and C. japonica genomes. 相似文献
3.
Genetic and physical mapping of <Emphasis Type="Italic">Pi36</Emphasis>(t), a novel rice blast resistance gene located on rice chromosome 8 总被引:12,自引:0,他引:12
Blast resistance in the indica cultivar (cv.) Q61 was inherited as a single dominant gene in two F2 populations, F2-1 and F2-2, derived from crosses between the donor cv. and two susceptible japonica cvs. Aichi Asahi and Lijiangxintuanheigu (LTH), respectively. To rapidly determine the chromosomal location of the resistance
(R) gene detected in Q61, random amplified polymorphic DNA (RAPD) analysis was performed in the F2-1 population using bulked-segregant analysis (BSA) in combination with recessive-class analysis (RCA). One of the three linked
markers identified, BA1126550, was cloned and sequenced. The R gene locus was roughly mapped on rice chromosome 8 by comparison of the BA1126550 sequence with rice sequences in the databases (chromosome landing). To confirm this finding, seven known markers, including
four sequence-tagged-site (STS) markers and three simple-sequence repeat (SSR) markers flanking BA1126550 on chromosome 8, were subjected to linkage analysis in the two F2 populations. The locus was mapped to a 5.8 cM interval bounded by RM5647 and RM8018 on the short arm of chromosome 8. This
novel R gene is therefore tentatively designated as Pi36(t). For fine mapping of the Pi36(t) locus, five additional markers including one STS marker and four candidate resistance gene (CRG) markers were developed
in the target region, based on the genomic sequence of the corresponding region of the reference japonica cv. Nipponbare. The Pi36(t) locus was finally localized to an interval of about 0.6 cM flanked by the markers RM5647 and CRG2, and co-segregated with
the markers CRG3 and CRG4. To physically map this locus, the Pi36(t)-linked markers were mapped by electronic hybridization to bacterial artificial chromosome (BAC) or P1 artificial chromosome
(PAC) clones of Nipponbare, and a contig map was constructed in silico through Pairwise BLAST analysis. The Pi36(t) locus was physically delimited to an interval of about 17.0 kb, based on the genomic sequence of Nipponbare. 相似文献
4.
Random-amplified-polymorphic DNA markers in sorghum 总被引:1,自引:0,他引:1
S. Pammi K. Schertz G. Xu G. Hart J. E. Mullet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(1):80-88
Conditions have been identified that allow reproducible amplification of RAPD markers in sorghum. High resolution of RAPD markers was accomplished by radiolabeling PCR-amplified DNAs followed by separation on denaturing 5% polyacrylamide gels. Reaction parameters including MgCl2 concentration and temperature significantly influenced yield and the type of amplification products synthesized. Unexplained amplified DNAs increased when more than 35 cycles of PCR amplification were used. Under standard conditions, approximately 80% of the primers tested amplified DNA, and most revealed 1–5 polymorphisms between BTx 623 and IS 3620C. Primers were used to amplify RAPDs in 32 genotypes of sorghum. In addition, 8 primers detected RAPDs in a population previously used to create an RFLP map for sorghum. These RAPDs were mapped successfully using a population of 50 F2 plants. 相似文献
5.
QTL-based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L.) 总被引:4,自引:0,他引:4
Abdelkhalik AF Shishido R Nomura K Ikehashi H 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(7):1226-1235
In order to identify quantitative trait loci (QTLs) for leaf senescence and related traits in rice (Oryza sativa L.), we developed two backcross populations, indica/japonica// japonica and indica/japonica//indica, using IR36 as the indica parent and Nekken-2 as the japonica parent. The QTLs were mapped using a set of simple sequence-repeat markers (SSRs) in the BC1F1 population. Senescence was characterized in these plants by measuring the leaf chlorophyll content 25 days after flowering (DAF), the reduction in chlorophyll content (the difference between the chlorophyll content at flowering and at 25 DAF), and the number of late-discoloring leaves per panicle at 25 DAF in five plants from each BC1F2 line. These plants were moved into a temperature-controlled growth cabinet at the time of flowering and allowed to mature under identical conditions. Eleven QTLs were detected in the two populations. The major of QTLs for senescence were found on the short arm of chromosome 6 and on the long arm of chromosome 9. Of these, one QTL on chromosome 6 and two on chromosome 9 were verified by confirming the effects of the genotypes on the phenotypes of the BC1F3 lines. The japonica parent was found to contribute to late senescence at all but one QTL. Based on a comparison of the effects of heterozygotes and homozygotes on the phenotypic values of each QTL genotype, we concluded that the differential senescence observed in the indica-japonica hybrid was not due to over-dominance; rather, it was the result of partial-dominance genes that were donated from either of the parents. 相似文献
6.
Genetic characterization and fine mapping of a novel thermo-sensitive genic male-sterile gene tms6 in rice (Oryza sativa L.) 总被引:5,自引:0,他引:5
Lee DS Chen LJ Suh HS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(7):1271-1277
The application of genetic male sterility in hybrid rice production has great potential to revolutionize hybrid seed production
methodology. The two-line breeding system by using thermo-sensitive genic male sterility (TGMS) has been discovered and successfully
developed as a breeding strategy in rice. One TGMS gene was investigated by a spontaneous rice mutant line, Sokcho-MS, originated
from a Korean japonica variety. It was shown that Sokcho-MS is completely sterile at a temperature higher than 27°C and/or lower than 25°C during
the development of spikelets, but fertile at the temperature ranging from 25 to 27°C regardless of the levels of day-length.
Genetic analysis and molecular mapping based on SSR, STS and EST markers revealed that a single recessive gene locus involved
the control of genic male sterility in Sokcho-MS. By using an F2 mapping population derived from a cross between Sokcho-MS and a fertile indica variety Neda, the new TGMS gene, designated as tms6, was mapped primarily to the long arm of chromosome 5 of Oryza sativa at the interval between markers E60663 (2.0 cM) and RM440 (5.8 cM). Subsequently, tms6 was fine mapped to the interval between markers RM3351 (0.1 cM) and E60663 (1.9 cM). As tms6 appeared to be independent of other mapped TGMS genes in rice, the genetic basis of Sokcho-MS was further discussed. 相似文献
7.
Fuli Liu Xiuliang Wang Jianting Yao Wandong Fu Delin Duan 《Journal of applied phycology》2010,22(1):109-111
Expressed sequence tag-derived microsatellite markers (EST-SSR) were generated and characterized in Laminaria japonica using data mining from updated public EST databases and polymorphism testing. Fifty-eight of 578 ESTs (10.0%) containing
various repeat motifs were used to design polymerase chain reaction (PCR) amplification primers. A total of 12 pairs of primer
were generated and used in the PCR amplification. Alleles per locus ranged from two to ten (average of 5.7). The observed
heterozygosities and expected heterozygosities were from 0.045 to 0.543 and from 0.056 to 0.814, respectively. All loci were
in Hardy–Weinberg equilibrium and no linkage disequilibrium was detected. These robust, informative, and potentially transferable
polymorphic markers appear suitable for population, genetic, parentage, and mapping studies of L. japonica. 相似文献
8.
Development and mapping of AFLP markers linked to the sorghum fertility restorer gene <Emphasis Type="Italic">rf4</Emphasis> 总被引:2,自引:2,他引:0
Wen L Tang HV Chen W Chang R Pring DR Klein PE Childs KL Klein RR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):577-585
The restoration of male fertility in the sorghum IS1112 C (A3) male-sterile cytoplasm is through a two-gene gametophytic system
involving complementary action of the restoring alleles Rf3 and Rf4. To develop markers suitable for mapping rf4, AFLP technology was applied to bulks of sterile and fertile individuals from a segregating BC3F1 population. Three AFLP markers linked to rf4 were identified and subsequently converted to STS/CAPS markers, two of which are co-dominant. Based on a population of 378
BC1F1 individuals, two STS/CAPS markers, LW7 and LW8, mapped to within 5.31 and 3.18 cM, respectively, of rf4, while an STS marker, LW9, was positioned 0.79 cM on the flanking side of rf4. Markers LW8 and LW9 were used to screen sorghum BAC libraries to identify the genomic region encoding rf4. A series of BAC clones shown to represent a genomic region of linkage group E were identified by the rf4-linked markers. A contig of BAC clones flanking the LW9 marker represent seed clones on linkage group E, from which fine
mapping of the rf4 locus and chromosome walking can be initiated.
Received: 20 June 2001 / Accepted: 3 August 2001 相似文献
9.
Chromosomal regions associated with marker segregation distortion in rice were compared based on six molecular linkage maps.
Mapping populations were derived from one interspecific backcross and five intersubspecific (indica / japonica) crosses, including two F2 populations, two doubled haploid (DH) populations, and one recombinant inbred (RI) population. Mapping data for each population
consisted of 129–629 markers. Segregation distortion was determined based on chi-square analysis (P < 0.01) and was observed at 6.8–31.8% of the mapped marker loci. Marker loci associated with skewed allele frequencies were
distributed on all 12 chromosomes. Distortion in eight chromosomal regions bracketed previously identified gametophyte (ga) or sterility genes (S). Distortion in three other chromosomal regions was found only in DH populations, where japonica alleles were over-represented, suggesting that loci in these regions may be associated with preferential regeneration of
japonica genotypes during anther culture. Three additional clusters of skewed markers were observed in more than one population in
regions where no gametophytic or sterility loci have previously been reported. A total of 17 segregation distortion loci may
be postulated based on this study and their locations in the rice genome were estimated.
Received: 31 May 1996 / Accepted: 30 September 1996 相似文献
10.
Li W Zeng R Zhang Z Ding X Zhang G 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(7):915-922
The partial pollen abortion of hybrids between the indica and japonica subspecies of Asian cultivated rice is one of the major barriers in utilizing intersubspecific heterosis in hybrid rice breeding.
Although a single hybrid pollen sterility locus may have little impact on spikelet fertility, the cumulative effect of several
loci usually leads to a serious decrease in spikelet fertility. Isolating of the genes conferring hybrid pollen sterility
is necessary to understand this phenomenon and to overcome the resulting genetic barrier. In this study, a new locus for F1 pollen sterility, S-d, was identified on the short arm of chromosome 1 by analyzing the genetic effect of substituted segments of the near-isogenic
line E11-5 derived from the japonica variety Taichung 65 (recurrent parent) and the indica variety Dee-geo-woo-gen (donor parent). The S-d locus was first mapped to a 0.8 cM interval between SSR markers PSM46 and PSM80 using a F2 population of 125 individuals. The flanking markers were then used to identify recombinants from a population of 2,160 plants
derived from heterozygotes of the primary F2 population. Simultaneously, additional markers were developed from genomic sequence divergence in this region. Analysis of
the recombinants in the region resulted in the successful mapping of the S-d locus to a 67-kb fragment, containing 17 predicted genes. Positional cloning of this gene will contribute to our understanding
of the molecular basis for partial pollen sterility of intersubspecific F1 hybrids in rice. 相似文献
11.
Genetic diversity of Cryptomeria japonica using co-dominant DNA markers based on sequenced-tagged sites 总被引:3,自引:0,他引:3
Y. Tsumura N. Tomaru 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(3-4):396-404
We have investigated the genetic diversity of 11 natural populations of C. japonica using 13 polymorphic STS markers. The average unbiased heterozygosities (H
e
), the average number of alleles per locus (N
a
) and the proportion of polymorphic loci (Pl) were 0.281, 1.93 and 76.92%, respectively. Coefficients of linkage disequilibrium were calculated, and no significant deviation
was found except in four combinations – which might have occurred by chance alone. The fixation index (F
IS
) for 3 loci showed statistically significant values at the 1% level. The genetic differentiation between populations was
only 0.047, and there were no clear geographical tendencies in the allele frequencies or the heterozygosities among populations.
Consequently, the results from STS-based co-dominant DNA marker analysis were very similar to those from a previous allozyme
study. However, the resolution of the technique is greater than allozyme analysis because many loci with high heterozygosities
can be evaluated, and it is very simple. Therefore, the STS-based marker approach is very useful and convenient for population
genetics and genome mapping of C. japonica.
Received: 18 July 1998 / Accepted: 13 August 1998 相似文献
12.
Isolation, characterization and mapping of simple sequence repeat markers in zoysiagrass (Zoysia spp.) 总被引:5,自引:0,他引:5
Cai HW Inoue M Yuyama N Takahashi W Hirata M Sasaki T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,112(1):158-166
The genus Zoysia consists of 16 species that are naturally distributed on sea coasts and grasslands around the Pacific. Of these, Zoysia japonica, Zoysia matrella, and Zoysia tenuifolia are grown extensively as turfgrasses, and Z. japonica is also used as forage grass in Japan and other countries in East Asia. To develop simple sequence repeat (SSR) markers for zoysiagrass (Zoysia spp.), we used four SSR-enriched genomic libraries to isolate 1,163 unique SSR clones. All four libraries contained a high percentage of perfect clones, ranging from 67.1 to 96.0%, and compound clones occurred with higher frequencies in libraries A (28.6%) and D (11.6%). From these clones, we developed 1,044 SSR markers when we tested all 1,163 SSR primer pairs. Using all 1,044 SSR markers, we tested one screening panel consisting of eight Zoysia clones for testing PCR amplifications, from which five unrelated clones, among the eight, were used for polymorphism assessment, and found that the polymorphic information content ranged from 0 (monomorphic loci) to 0.88. Of the 1,044 SSR markers, 170 were segregated in our mapping population and we mapped 161 on existing amplified fragment length polymorphism-based linkage groups, using this mapping population. These SSR markers will provide an ideal marker system to assist with gene targeting, quantitative trait locus mapping, variety or species identification, and marker-assisted selection in Zoysia species.Electronic supplementary material Supplementary material is available in the online version of this article at
and is accessible for authorized users. 相似文献
13.
Identification and mapping of a new leaf stripe resistance gene in barley (Hordeum vulgare L.) 总被引:2,自引:0,他引:2
G. Tacconi L. Cattivelli N. Faccini N. Pecchioni A. M. Stanca G. Valé 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1286-1291
Pyrenophora graminea is the seed-borne pathogen causal agent of barley leaf stripe disease. Near-isogenic lines (NILs) carrying resistance of
the cv ”Thibaut” against the highly virulent isolate Dg2 were obtained by introgressing the resistance into the genetic background
of the susceptible cv ”Mirco”. The segregation of the resistance gene was followed in a F2 population of 128 plants as well as on the F3 lines derived from the F2 plants; the segregation fitted the 1:2:1 ratio for a single gene. By using NILs, a RAPD marker associated with the resistance
gene was identified; sequence-specific (STS) primers were designed on the basis of the amplicon sequence and a RILs mapping
population with an AFLP-based map were used to position this molecular marker to barley chromosome 1 S (7HS). STS and CAPS
markers were developed from RFLPs mapped to the telomeric region of barley chromosome 7HS and three polymorphic PCR-based
markers were developed. The segregation of these markers was followed in the F2 population and their map position with respect to the resistance gene was determined. Our results indicate that the Thibaut
resistance gene, which we designated as Rdg2a, maps to the telomeric region of barley chromosome 7HS and is flanked by the markers OPQ-9700 and MWG 2018 at distances of 3.1 and 2.5 cM respectively. The suitability of the PCR-based marker MWG2018 in selection- assisted
barley breeding programs is discussed.
Received: 22 June 2000 / Accepted: 16 October 2000 相似文献
14.
Identification and mapping of pm2026: a recessive powdery mildew resistance gene in an einkorn (Triticum monococcum L.) accession 总被引:1,自引:1,他引:0
Xu H Yao G Xiong L Yang L Jiang Y Fu B Zhao W Zhang Z Zhang C Ma Z 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(4):471-477
Triticum monococcum accession TA2026 showed resistance to wheat powdery mildew. To identify the resistance gene and transfer it to common wheat,
genetic analysis and molecular mapping were conducted using an F2 population and derived F3 families from the cross of TA2026 × M389. The results indicated that TA2026 possessed a recessive powdery mildew resistance
gene. This gene was mapped to the terminal portion of chromosome 5AmL and flanked by SSR marker loci Xcfd39 and Xgwm126. Eight RFLP markers previously mapped to the terminal chromosome 5AmL were converted into STS markers. Three loci, detected by MAG1491, MAG1493 and MAG1494, the STS markers derived from RFLP
probes CDO1312, PSR164 and PSR1201, respectively, were linked to this resistance gene with Xmag1493 only 0.9 cM apart from it. In addition, the STS marker MAG2170 developed from the tentative consensus wheat cDNA encoding
the Mlo-like protein identified a locus co-segregating with Xmag1493. This is the first recessive powdery mildew resistance gene identified on chromosome 5Am, and is temporarily designated pm2026. We have successfully transferred it to a tetraploid background, and this resistance stock will now be used as the bridge
parent for its transfer to common wheat. 相似文献
15.
Yongfu Qiu Jianping Guo Shengli Jing Lili Zhu Guangcun He 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(8):1601-1611
Brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive insect pests of rice. Exploring resistance genes from diverse germplasms and incorporating them into cultivated varieties are critical for controlling this insect. The rice variety Swarnalata was reported to carry a resistance gene (designated Bph6), which has not yet been assigned to a chromosome location and the resistance mechanism is still unknown. In this study, we identified and mapped this gene using the F2 and backcrossing populations and characterized its resistance in indica 9311 and japonica Nipponbare using near isogenic lines (NILs). In analysis of 9311/Swarnalata F2 population, the Bph6 gene was located on the long arm of chromosome 4 between the SSR markers RM6997 and RM5742. The gene was further mapped precisely to a 25-kb region delimited between the STS markers Y19 and Y9; and the distance between these markers is 25-kb in Nipponbare genome. The Bph6 explained 77.5% of the phenotypic variance of BPH resistance in F2 population and 84.9% in BC2F2 population. Allele from Swarnalata significantly increased resistance to the BPH, resulted in a reduced damage score. In characterization of Bph6-mediated resistance, the BPH insects showed significant preference between NIL-9311 and 9311 in 3 h and between NIL-NIP and Nipponbare in 120 h after release. BPH growth and development were inhibited, and the insect’s survival rates were lower on Bph6-NIL plants, compared with the parents 9311 and Nipponbare. The results indicate that the Bph6 exerted prolonged antixenotic and antibiotic effects in Bph6-NIL plants, and NIL-9311 plants showed a quicker and stronger effect toward BPH than NIL-NIP plants. 相似文献
16.
Bing ZHAO Qi-Ming DENG Qi-Jun ZHANG Jie-Qin LI Shao-Ping YE Yong-Shu LIANG Yong PENG Ping LI 《Acta Genetica Sinica》2006,33(5):449-457
A genetic linkage map comprising 148 SSR markers loci was constructed using an F2 population consisting of 90 lines derived from a sub-specific cross between a japonica variety Nipponbare and an indica variety Guangluai-4. The F2 population showed high significantly distorted segregations. Among these SSR markers, 49 markers (33.11%) showed the genetics distortion(P<0.05). Of them, 36 markers deviated toward male parent indica GuangLuAi-4 and 13 markers toward heterozygote, but none toward the female parent Nipponbare. It was found that the segregation distortion might be caused by gametophyte and zygote. Since most gametophyte loci and sterility loci were mapped in segregation distortion regions, it indicated that the segregation distortion may be caused by these gametophyte loci and sterility loci. Finally, this research also analyzed the skewed segregation of some markers, which had not been mapped on chromosome. 相似文献
17.
The molecular characteristics of markers in the chromosome region surrounding the supernodulation gene (nts-1) of soybean (Glycine max L. Merr.) were investigated in 187 F2 plants from a cross of G. max cv. Bragg (nts) and G. soja PI468.397 (wild-type nodulation). RFLP marker pUTG-132a, linked tightly (0.7±0.5 cM) to nts-1, was converted to a PCR marker. The polymorphism resides within a 1.72 kb PstI fragment and consists of an 832 bp insertion in G. max relative to the wild progenitor G. soja. The insertion is flanked by a 35 bp direct duplication that was found only once in G. soja. Data suggest that the pUTG-132a sequence exists only once in the genome, which is compatible with the recessive nature of
nts-1. Accordingly, pUTG-132a is a valuable marker for map-based cloning. Another RFLP marker, pA-381, was mapped 4.8 cM distal
to nts-1. Marker order, established by Maximum Likelihood Analysis, placed nts-1 between pUTG-132a and pA-381. To generate additional molecular markers, a segregating F2 population was analysed using bulked segregant analysis (BSA) and single oligonucleotide primer-based PCR (DNA amplification
fingerprinting; DAF). PCR marker pcr5-4L was mapped to soybean linkage group H and sequenced. The data revealed (i) recombination
events and marker order in the nts-1 region; (ii) the molecular nature and cause of polymorphisms in linked molecular markers; (iii) a low density of polymorphisms
around nts-1, and (iv) diploidy of the distal region of linkage group H of soybean.
Received: 18 January 1996 / Accepted: 9 October 1996 相似文献
18.
Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat 总被引:2,自引:0,他引:2
Yu J Herrmann M 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(3):429-437
The powdery mildew resistance from Avena macrostachya was successfully introgressed into hexaploid oat (A. sativa). Genetic analysis of F1, F2, F3 and BC1 populations from two powdery-mildew resistant introgression lines revealed that the resistance is controlled by a dominant gene, tentatively designated Eg-5. Molecular marker analysis was conducted using bulked-segregant analysis in two segregating F3 populations. One codominant simple sequence repeats (SSR) marker AM102 and four AFLP-derived PCR-based markers were successfully developed. The SSR marker AM102 and the STS marker ASE41M56 were linked to the gene Eg-5, with genetic distances of 2 and 0.4 cM, respectively, in both mapping populations. Three STS markers (ASE45M56, ASE41M61, ASE36M55) co-segregated with Eg-5 in one population while two (ASE45M56, ASE36M55) of them linked to Eg-5 with a genetic distance of 1 cM in another population. The gene was further mapped to be in a region corresponding to linkage group 22_44+18 in the Kanota × Ogle (KO) hexaploid oat map by comparative mapping. To our knowledge, this is the first report of mapping powdery-mildew resistance in hexaploid oat. The new resistance source of A. macrostachya, together with the tightly linked markers identified here, could be beneficial in oat breeding programmes. 相似文献
19.
Fine mapping of the rice thermo-sensitive genic male-sterile gene <Emphasis Type="Italic">tms5</Emphasis> 总被引:10,自引:0,他引:10
Wang YG Xing QH Deng QY Liang FS Yuan LP Weng ML Wang B 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(5):917-921
AnnongS-1, a thermo-sensitive genic male-sterile (TGMS) rice line, has a new TGMS gene. Genetic analysis indicated that the sterility of AnnongS-1 was controlled by a single resessive gene named tms5. In our previous studies based on an F2 population from the cross between AnnongS-1 and Nanjing11, tms5 was mapped on chromosome 2. Recently, a RIL (recombinant inbred line) population from the same cross was developed and used for the fine mapping of the tms5 gene. Molecular marker techniques combined with BSA (bulked segregant analysis) were used. As a result, two AFLP markers (AF10, AF8), one RAPD marker (RA4), one STS marker (C365-1), one CAPs marker (G227-1) and four SSR markers (RM279, RM492, RM327, RM324) were found to be closely linked to tms5 gene. The DNA sequences of the RFLP marker of C365 and G227 were found in GenBank, and on the basis of these sequences, many primers were designed to amplify the two parents and their RIL population plants. Finally, the tms5 gene was mapped between STS marker C365-1 and CAPs marker G227-1 at a distance of 1.04 cM from C365-1 and 2.08 cM from G227-1.Communicated by H.F. LinskensY.G. Wang and Q.H. Xing contributed equally to this contribution. 相似文献
20.
Hayase Shisa Lingmin Lu Hideki Katoh Atsuko Kawarai Jun-ichi Tanuma Yoshibumi Matsushima Hiroshi Hiai 《Mammalian genome》1997,8(5):324-327
A new set of rat RI strains consisting of 11 independent strains and 13 of their substrains was established by inbreeding
F2 rats between F344/DuCrj and LE/Stm. The strain distribution pattern was examined for 66 microsatellite loci, 8 biochemical
genetic markers, 2 histocompatibility loci, and 2 coat color genes. A rat salivary protein gene Spe1 was newly mapped on Chr 1.
Received: 13 August 1996 / Accepted: 23 December 1996 相似文献