共查询到20条相似文献,搜索用时 15 毫秒
1.
Vyacheslav P. Pankratov Yuri A. Mantzygin Oleg S. Morenkov Natal'ya V. Swyatukhina 《Biotechnology letters》1985,7(3):141-146
Summary Mouse hybridoma cells were grown on surface of glass disks in a cell reactor. It is shown that during continuous cultivation the cells retain their antibody-producing ability. The yield of the culture supernatant obtained in the reactor exceeds about 5 fold those obtained in flasks with equivalent area. 相似文献
2.
Conditions were found in which Chlamydomonas reinhardi exhibits a circadian alteration of its cell surface, measured as ability to stick to glass. Under these same conditions the cells also show circadian rhythms of cell division and release of daughter cells. The three rhythmic phenomena were shown to have typical properties of rhythms controlled by the biological clock. The rhythm of stickiness was used to demonstrate that in a mixed culture containing two cell populations with natural periods differing by 2 to 3 hours, the cells did not mutally entrain each other and that this rhythm could be successfully applied in an enrichment procedure for mutants of the biological clock. Stickiness was shown to be independent of growth and motility of the cells and unaffected by red or far red illimination. Minimally sticking cells did not affect the sticking of maximally sticking cells in a mixed culture; nor was there a progressive increase in stickiness shown at the minimum from one cycle to the next in a pure culture. These results indicate that sticking probably is not mediated by long lived adhesive material or enzymes excreted into the medium. Several tests of the sensitivity of stickiness to replacement of the growth medium by distilled water or water containing various compounds suggest that ions might play an important role in the sticking reaction. 相似文献
3.
Fine structure of human malaria in vitro. 总被引:15,自引:0,他引:15
The erythrocytic cycle of the human malaria parasite, Plasmodium, falciparum, was examined by electron microscopy. Three strains of parasites maintained in continuous culture in human erythrocytes were compared with in vivo infections in Aotus monkeys. The ultrastructure of P. falciparum is not altered by continuous cultivation in vitro. Mitochondria contain DNA-like filaments and some cristae at all stages of the erythrocytic life cycle. The Golgi apparatus is prominent at the schizont stage and may be involved in the formation of rhoptries. In culture, knob-like protrusions first appear on the surface of trophozoite-infected erythrocytes. The time of appearance of knobs on cells in vitro correlates with the life cycle stage of parasites which are sequestered from the peripheral circulation in vivo. Knob material of older parasites coalesces and forms extensions from the erythrocyte surface. Some of this material is sloughed from the host cell surface. The parasitophorous vacuole membrane breaks down in erythrocytes containing mature merozoites both in vitro and in vivo. Merozoite structure is similar to that of P. knowlesi. The immature gametocytes in culture have no knobs. 相似文献
4.
Surface structure changes of rat adipocytes during lypolysis stimulated by various lypolysis stimulated by various lypolytic agents
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A qualitative and quantitative electron microscopic study was performed on rat adipocytes during stimulation of lipolysis by various agents. Scanning electron microscopy of control cells revealed a spherical cell with a textured glycocalyx surface exhibiting small irregular projections. Globular surface evaginations or protrusions measuring 8-18 μM in diameter were seen on cell hemispheres, and there was an average of one protrusion for every two hemispheres examined. Distribution analysis showed that 60 percent of the hemispheres had no protrusions, and 25, 10, and 5 percent of the hemispheres had one, two or three protrusions, respectively. Thin-section and freeze- fracture electron microscopy of the protrusions showed a small triglyceride droplet surrounded by a thin cytoplasmic rim that was continuous with the main cytoplasmic matrix. The glycocalyx coating and plasma membrane extended from the cell surface onto, and over, the protrusion. Scanning microscopy of cells stimulated by lipolytic agents, including epinephrine, adrenocorticotropic hormone, theophylline, and dibutyryl cyclic AMP, revealed a dose-dependent increase in the number of protrusions per cell hemisphere. Maximal concentrations of lipolytic hormones cuase an average 2.5-fold increase in the number of protrusions per hemisphere without changing the average size of the protrusions. Only 40 percent of the stimulated cell hemispheres exhibited no protrusions; over 15 percent of the cells contained three or more; and a number of the protrusions were multilobulate. Insulin prevented the increase in the number of protrusions and the change in distribution caused by the lipolytic hormones but did not prevent the increase caused by theophylline and dibutryl cyclic AMP. The data suggest that the protrusions are a structural feature of the cell and may be related to the lypolytic pathway. These observations may help explain some of the discrepant biochemical data relating to hormonal stimulation of lipolysis. 相似文献
5.
Rodionova NA Dubovaia NV Martinovich LI Bezborodov AM 《Prikladnaia biokhimiia i mikrobiologiia》2001,37(5):562-565
The activities of extracellular systems of hemicellulases, pectinases, and cellulases was studied during a 72-h cultivation of Geotrichum candidum 3C. The culture was grown on a medium containing 3% cell walls isolated from wheat grain capsules, which served as the sole carbon source. Enzymes catalyzing the degradation of pectin substances (beet pectin, alpha-L-arabinan, and 1,4-beta-D-galactan), as well as beta-D-galactosidase and alpha-L-arabinofuranosidase involved in their hydrolysis, were formed first (4 h after the beginning of cultivation). Enzymes hydrolyzing 4-O-methyl-alpha-D-glucurono-beta-D-xylan and sodium carboxymethyl xylan were also found in the culture liquid after 4 h of fungal growth. The contents of pectin-degrading and xylanolytic enzymes reached their maximum levels after 52-56 and 72 h of growth, respectively. Cellulolytic enzymes were detected after 8-28 h of cultivation. Enzymes degrading alpha-D-galacto-beta-D-mannan were found 24 h after the beginning of growth; their content was maximum after 72 h of cultivation. 相似文献
6.
Two--five-day-old culture of 10-11-day-old chick embryos has been used. Antiserum against galactocerebrosides (anti-GalC) is added to the nutrition medium before cultuvation. In the presence of anti-GalC the growth and gliocyte migration zone decreases, the area index becomes essentially small (3.5 +/- 0.7 in the control, 1.5 +/- 0.5 in the experiment). This is connected with inhibition of migration and proliferation of gliocytes during first 48 h of cultivation, while intensity of neuron regeneration remains unchanged. Alterations of the neurit-glial relations are investigated by means of the vital phase-contrast microscopy. Effect of anti-GalC to peripheral gliocytes is accompanied with a decreasing adhesive ability of their plasmolemma. This makes difficult their flattening on the neurit membrane, formation of contact membranous neurit-glial relations and formation of glial membranes. On the 3d day formation of nervous fasciculi is retarded, as well as their fusion into trunks and plexuses. On the 5th day, unlike the control, a continuous "epineural" covering of neuritic plexuses does not form. Round retractile and defective (with protrusions) forms of gliocytes predominate. These data demonstrate inhibitory effect of anti-GalC on the structural-functional maturation of the glia and on formation of neurit-glial relations in the culture of the sensitive ganglion. 相似文献
7.
N F Ostapenko E I Lezhnev V P Lavrovskaia V M Girshgorn V P Borshchov 《Tsitologiia》1985,27(11):1320-1324
The growth of BHK-21 culture cells on the outer surface and in the clear space of capillaries made of phenylone or polyacrylnitrile was studied. The cultivation of cells in the clear space of the capillary extends their survival time and outlines possible ways of continuous cultivation of tissue cells which are cultivated in the attached state. 相似文献
8.
Fibrobacter succinogenes subsp. succinogenes S85 initiated growth on microcrystalline cellulose without a lag whether inoculated from a glucose, cellobiose, or cellulose culture. During growth on cellulose, there was no accumulation of soluble carbohydrate. When the growth medium contained either glucose or cellobiose in combination with microcrystalline cellulose, there was a lag in cellulose digestion until all of the soluble sugar had been utilized, suggesting an end product feedback mechanism that affects cellulose digestion. Cl-stimulated cellobiosidase and periplasmic cellodextrinase were produced under all growth conditions tested, indicating constitutive synthesis. Both cellobiosidases were cell associated until the stationary phase of growth, whereas proteins antigenically related to the Cl-stimulated cellobiosidase and a proportion of the endoglucanase were released into the extracellular culture fluid during growth, irrespective of the substrate. Immunoelectron microscopy of cells with a polyclonal antibody to Cl-stimulated cellobiosidase as the primary antibody and 10-nm-diameter gold particles conjugated to goat anti-rabbit antibodies as the second antibody revealed protrusions of the outer surface which were selectively labeled with gold, suggesting that Cl-stimulated cellobiosidase was located on the protrusions. These data support the contention that the protrusions have a role in cellulose hydrolysis; however, this interpretation is complicated by reactivity of the antibodies with a large number of other proteins that possess related antigenic epitopes. 相似文献
9.
Cellulose digestion and cellulase regulation and distribution in Fibrobacter succinogenes subsp. succinogenes S85. 总被引:5,自引:3,他引:2
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Fibrobacter succinogenes subsp. succinogenes S85 initiated growth on microcrystalline cellulose without a lag whether inoculated from a glucose, cellobiose, or cellulose culture. During growth on cellulose, there was no accumulation of soluble carbohydrate. When the growth medium contained either glucose or cellobiose in combination with microcrystalline cellulose, there was a lag in cellulose digestion until all of the soluble sugar had been utilized, suggesting an end product feedback mechanism that affects cellulose digestion. Cl-stimulated cellobiosidase and periplasmic cellodextrinase were produced under all growth conditions tested, indicating constitutive synthesis. Both cellobiosidases were cell associated until the stationary phase of growth, whereas proteins antigenically related to the Cl-stimulated cellobiosidase and a proportion of the endoglucanase were released into the extracellular culture fluid during growth, irrespective of the substrate. Immunoelectron microscopy of cells with a polyclonal antibody to Cl-stimulated cellobiosidase as the primary antibody and 10-nm-diameter gold particles conjugated to goat anti-rabbit antibodies as the second antibody revealed protrusions of the outer surface which were selectively labeled with gold, suggesting that Cl-stimulated cellobiosidase was located on the protrusions. These data support the contention that the protrusions have a role in cellulose hydrolysis; however, this interpretation is complicated by reactivity of the antibodies with a large number of other proteins that possess related antigenic epitopes. 相似文献
10.
《Journal of Fermentation Technology》1988,66(6):643-647
l-Methionine-enriched cells production of an ethionine-resistant mutant of Candida boidinii no. 2201 was greatly improved by the control of pH and by feeding of methanol and other medium components during cultivation in a jar fermentor. Under the optimal conditions, 38.5 g (as dry weight)_of cells abd 282 mg of pool methionine (intracellular pool of free l-methionine) per l of culture broth were obtained after 11 d of cultivation.The culture conditions for production of l-methionine-enriched cells in continuous culture were investigated. With limited methanol in continuous cultivation, pool methionine productivity reached a maximum value of 1.14 mg·l−1·h−1 at a dilution rate of 0.05·h−1. During methanol-limited growth in continuous cultivation, the pool methionine content of the mutant was about 20–35% higher than that in batch cultivation. 相似文献
11.
The dynamics of glucose metabolites production by Escherichia coli CM 5199 was studied under the conditions of batch and continuous cultivation. Acetate and ethanol were shown to be accumulated in the cultural broth in considerable amounts, and the rate of their synthesis was directly proportional to the specific growth rate of the culture. Acetate inhibited E. coli growth as was found in experiments conducted in a turbidostat regimen. As a result, the specific growth rate of the strain decreased during both batch and continuous cultivation. 相似文献
12.
R Ia Makhmutov 《Zhurnal evoliutsionno? biokhimii i fiziologii》1989,25(3):337-340
Studies have been made on the pattern of cell behaviour and distribution of cellular projections during stabilization of neuroglial nets in the cortex of newborn rat puppies under the conditions of tissue culture. Observations were made on surviving cells at the 3rd day of cultivation. Projection growth and changes in cell location were registered microphotographically within a day with a 4-hour interval. It was found that together with relative stability of their location, cells exhibit translocations in various directions up to a distance of several cellular diameters. Projection growth in the vicinity of cells occurs mainly in a rhythmic pattern. 相似文献
13.
The present paper describes an attempt to maintain the metatarsal and caudal glands of deer sambar (Cervus unicolor Kerr) in diffusion chambers. Another purpose was to provide information on the secretory granule of these glands. Our results show that cultured gland cells have the capacity to reassociate into islet-like organoids in diffusion chambers. Competence in some epithelial cell types may probably be regulated as a function of growth. Scanning electron microscopy of secretory cells showed that the cells in the culture often had numerous short filopodia and blebs. The secretory granules of the caudal gland were spherical and uniform in size. Some of the secretory granules had pseudopodia-like protrusions. By means of SEM, the present study provides evidence of rapid structural changes in the surfaces of epithelial cells in the course of cultivation. It has been suggested that the structural organization in the cell colonies serves to "guide" the function of secretory cells. 相似文献
14.
Gramicidin S biosynthesis was studied in Bacillus brevis var. G.-B. during its batch and continuous cultivation when the culture growth was limited with nutrient sources (glycerol, ammonium nitrogen, phosphate), oxygen deficiency and the action of a physical factor (a low temperature). The antibiotic biosynthesis was shown to be induced by a change in the growth rate caused by the action of any factor decelerating the growth. The authors propose a mathematical model for the antibiotic synthesis, biomass accumulation and the utilization of a substrate limiting the growth. The model is based on the age separation of cells. The model is analyzed in terms of optimizing the one-stage continuous cultivation process. The model allows one to calculate optimal conditions of the antibiotic synthesis in the process of one-stage continuous cultivation. 相似文献
15.
The surface morphology of chick myogenic cells during development in cell culture was examined by scanning electron microscopy. Myoblasts at the G1 and S phases of the cell cycle had a relatively smooth surface. In late G2 and mitosis, they had many microvilli and some blebs on their surfaces. Ca2+ -deficient fusion-arrested myoblasts had a relatively smooth surface. When the cells underwent cell fusion, many microvilli, small spherical protrusions, and some blebs appeared on their surface. In newly formed myotubes, the surface over the nucleus was smooth whereas that over perinuclear regions had many flat excrescences and other surface protrusions. This mosaic appearance of the surface was less prominent in striated myotubes. Scanning electron microscopy combined with fluorescence microscopy using rhodamine-labeled erabutoxin b revealed that sites of accumulation of acetylcholine receptor had a smooth surface. These results suggest that changes in surface structure occur in association with the cell cycle, fusion and subsequent development of myotubes. 相似文献
16.
The physiology of growth under the conditions of batch and continuous cultivation was studied with the recombinant strain of Escherichia coli CM 5199 capable of DNA polymerase I superproduction. The specific growth rate of the strain is 0.8 h-1 under the conditions of continuous cultivation which is almost 2.5 times greater than that in the exponential phase of batch cultivation. When the strain was cultivated at a flow rate above 0.3 h-1, the biomass concentration in the fermenter decreased and the culture was no more limited by the carbon source in the absence of other growth limiting components of the medium. Apparently, the metabolic product ceased to inhibit high growth rates of the culture under the conditions of continuous cultivation. The rate of DNA polymerase synthesis correlated with the specific growth rate and the respiration activity of the culture when the lambda pol A prophage was induced in the cells. The authors discuss the effectiveness of ribosome operation in the cells at a growth rate of 0.05 to 0.3 h-1 and the content of ribosomes at a higher growth rate in relation to DNA polymerase I synthesis. 相似文献
17.
Specka U Spreinat A Antranikian G Mayer F 《Applied and environmental microbiology》1991,57(4):1062-1069
Clostridium thermosulfurogenes EM1 formed blebs, i.e., protrusions still in contact with the cytoplasmic membrane, that originated from the cytoplasmic membrane during growth in batch culture and continuous culture. They could be observed squeezed between the cell wall and cytoplasmic membrane in cells with seemingly intact wall layers (surface layer and peptidoglycan layer) as well as in cells with wall layers in different states of degradation caused by phosphate limitation or high dilution rates. Blebs were found to turn into membrane vesicles by constriction in cases when the cell wall was heavily degraded. Bleb and vesicle formation was also observed in the absence of substrates that induce alpha-amylase and pullulanase synthesis. No correlations existed between bleb formation and the presence of active enzyme. Similar blebs could also be observed in a number of other gram-positive bacteria not producing these enzymes, but they were not observed in gram-negative bacteria. For immunoelectron-microscopic localization of alpha-amylase and pullulanase in C. thermosulfurogenes EM1, two different antisera were applied. One was raised against the enzymes isolated from the culture fluid; the other was produced against a peptide synthesized, as a defined epitope, in analogy to the N-terminal amino acid sequence (21 amino acids) of the native extracellular alpha-amylase. By using these antisera, alpha-amylase and pullulanase were localized at the cell periphery in samples taken from continuous culture or batch culture. In samples prepared for electron microscopy by freeze substitution followed by ultrathin sectioning, blebs could be seen, and the immunolabel pinpointing alpha-amylase enzyme particles was seen not only randomly distributed in the cell periphery, but also lining the surface of the cytoplasmic membrane and the blebs. Cells exhibiting high or virtually no enzyme activity were labeled similarly with both antisera. This finding strongly suggests that alpha-amylase and pullulanase may occur in both active and inactive forms, depending on growth conditions. 相似文献
18.
Cell wall synthesis in carrot cells: Comparison of suspension-cultured cells and regenerating protoplasts 总被引:1,自引:0,他引:1
The synthesis of cell walls and extracellular material during the regeneration of carrot ( Daucus carota L. ssp. sativus ) protoplasts was examined. Cell walls and extracellular material were analysed for their carbohydrate content. In cell walls, the amount of carbohydrate increased 4- to 5-fold with only minor changes in neutral sugar composition. Glucose was abundant, during cultivation, making up to 70% (w/w) followed by mannose, which accounted for 17%. This indicates the formation of a glucomannan. The neutral sugar composition of extracellular polysaccharides showed greater variety with a signifiant increase of arabinose and galactose during cultivation. This feature is probably connected to the occurrence of arabinogalactan proteins in the culture medium. Hydroxyproline, an indicator for extensin and arabinogalactan proteins, showed an increase parallel to the formation of cell walls and extracellular polysaccharides. Results are compared with corresponding data from suspension-cultured cells used for protoplast isolation. 相似文献
19.
N W Charon S F Goldstein S M Block K Curci J D Ruby J A Kreiling R J Limberger 《Journal of bacteriology》1992,174(3):832-840
We recently characterized the three-dimensional shape of Treponema phagedenis periplasmic flagella (PFs). In the course of these studies, we observed protrusions on swimming cells that resembled PFs. Here we present a detailed characterization of the shape, structure, and motion of these protrusions. Although protrusion formation occurred primarily in wild-type cells during the stationary phase, a large fraction of exponential-phase cells of cell cylinder helicity mutants (greater than 90% of mutant T-52) had protrusions. These results suggest that cells bearing protrusions can still participate in cell division. T. phagedenis protrusions had the identical helix handedness, pitch, and diameter to those of purified PFs. Protrusions were not present on mutants unable to synthesize PFs, but were present in all motile revertants which regained PFs. These results, taken together with electron microscope observations, suggest that protrusions consist of PFs surrounded by an outer membrane sheath. To analyze protrusion movements, we held cells against a coverglass surface with optical tweezers and observed the motion of protrusions by video-enhanced differential interference contrast light microscopy. Protrusions were found to gyrate in both clockwise and counterclockwise directions, and direct evidence was obtained that protrusions rotate. Protrusions were also observed on Treponema denticola and Borrelia burgdorferi. These were also left-handed and had the same helix handedness, pitch, and diameter as purified PFs from their respective species. The PFs from T. denticola had a helix diameter of 0.26 microns and a helix pitch of 0.78 micron; PFs from B. burgdorferi had a helix diameter of 0.28 micron and a helix pitch of 1.48 microns. Protrusions from these spirochete species had similar structures and motion to those of T. phagedenis. Our results present direct evidence that PFs rotate and support previously proposed models of spirochete motility. 相似文献
20.
Wolfgang E. Simon Marianne Hänsel Manfred Dietel Lenchen Matthiesen Michael Albrecht Fritz Hölzel 《In vitro cellular & developmental biology. Plant》1984,20(3):157-166
Summary During early cultivation steps of the newly derived and karyotyped human mammary carcinoma line EFM-19, the cells developed
faster growth rates and became increasingly less responsive to the presence of serum in the culture medium. No drastic alterations
of the morphology and of the karyotype were observed, and carcinoembryogenic antigen remained expressed during the course
of the cultivation. In experimental incubations at various time intervals after the explantation, the cell proliferation was
analyzed for dose-dependent effects of estradiol, cortisol, progesterone, and testosterone. After 16 wk of cultivation of
the stock culture in the presence of estradiol, the cells had acquired a distinct sensitivity to estradiol resulting in permanent
growth enhancement. The withdrawal of cortisol from the medium of the stock culture subsequently provoked the loss of the
initially noted stimulation of the proliferation by cortisol. The stimulatory effect of progesterone on the proliferation
was reversed to inhibition when the stock culture was deprived of cortisol in the growth medium. The results indicate that
the choice of steroid hormones in the stock culture medium was determining the quality of the cellular growth responses.
This work was supported by the Deutsche Forschungsgemeinschaft, SFB34. 相似文献