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Overview of vector design for mammalian gene expression 总被引:4,自引:0,他引:4
Kaufman RJ 《Molecular biotechnology》2000,16(2):151-160
The expression of cloned genes in mammalian cells is a basic tool for understanding gene expression, protein structure, and
function, and biological regulatory mechanisms. The level of protein expression from heterologous genes introduced into mammlaian
cells depends upon multiple factors including DNA copy number, efficiency of transportation, mRNA processing, mRNA transport,
mRNA stability, and translational efficiency, and protein processing, transport, and stability. Different genes exhibit different
rate limiting steps for efficient expression. Multiple strategies are available to obtain high level expression in mammalian
cells. This article reviews vector design for expression of foreign genes in mammalian cells. 相似文献
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