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1.
Derong Hao Zhenliang Zhang Yujing Cheng Guoqing Chen Huhua Lu Yuxiang Mao Mingliang Shi Xiaolan Huang Guangfei Zhou Lin Xue 《PloS one》2015,10(11)
Waxy maize (Zea mays L. var. ceratina) is an important vegetable and economic crop that is thought to have originated from cultivated flint maize and most recently underwent divergence from common maize. In this study, a total of 110 waxy and 110 common maize inbred lines were genotyped with 3072 SNPs to evaluate the genetic diversity, population structure, and linkage disequilibrium decay as well as identify putative loci that are under positive selection. The results revealed abundant genetic diversity in the studied panel and that genetic diversity was much higher in common than in waxy maize germplasms. Principal coordinate analysis and neighbor-joining cluster analysis consistently classified the 220 accessions into two major groups and a mixed group with mixed ancestry. Subpopulation structure in both waxy and common maize sets were associated with the germplasm origin and corresponding heterotic groups. The LD decay distance (1500–2000 kb) in waxy maize was lower than that in common maize. Fourteen candidate loci were identified as under positive selection between waxy and common maize at the 99% confidence level. The information from this study can assist waxy maize breeders by enhancing parental line selection and breeding program design. 相似文献
2.
Information regarding the genetic diversity and genetic relationships among elite inbred lines is necessary to improve new cultivars in maize breeding programs. In this study, genetic diversity and genetic relationships were investigated among 84 waxy maize inbred lines using 50 SSR markers. A total of 269 alleles were identified at all the loci with an average of 5.38 and a range between 2 and 13 alleles per locus. The gene diversity values varied from 0.383 to 0.923 with an average of 0.641. The cluster tree generated using the described SSR markers recognized two major groups at 32% genetic similarity. Group I included 33 inbred lines while group II included 51 inbred lines. The clustering patterns of most of the waxy maize inbred lines did not clearly agree with their source, pedigree or geographic location. The average GS among all inbred lines was 35.7 ± 10.8. Analysis of waxy maize inbred lines collected from Korea and China at 50 SSR loci revealed higher values of average number of alleles (4.9) and gene diversity (0.638) in Korean inbred lines as compared to Chinese inbred lines (3.5 and 0.563, respectively). The information obtained from the present studies would be very useful for maize breeding programs in Korea. 相似文献
3.
Post-Domestication Selection in the Maize Starch Pathway 总被引:1,自引:0,他引:1
Longjiang Fan Jiandong Bao Yu Wang Jianqiang Yao Yijie Gui Weiming Hu Jinqing Zhu Mengqian Zeng Yu Li Yunbi Xu 《PloS one》2009,4(10)
Modern crops have usually experienced domestication selection and subsequent genetic improvement (post-domestication selection). Chinese waxy maize, which originated from non-glutinous domesticated maize (Zea mays ssp. mays), provides a unique model for investigating the post-domestication selection of maize. In this study, the genetic diversity of six key genes in the starch pathway was investigated in a glutinous population that included 55 Chinese waxy accessions, and a selective bottleneck that resulted in apparent reductions in diversity in Chinese waxy maize was observed. Significant positive selection in waxy (wx) but not amylose extender1 (ae1) was detected in the glutinous population, in complete contrast to the findings in non-glutinous maize, which indicated a shift in the selection target from ae1 to wx during the improvement of Chinese waxy maize. Our results suggest that an agronomic trait can be quickly improved into a target trait with changes in the selection target among genes in a crop pathway. 相似文献
4.
基于DUS测试性状的玉米自交系形态多样性分析 总被引:2,自引:2,他引:2
利用DUS测试中的47个性状对15份普通玉米自交系和13份糯玉米骨干自交系进行了形态多样性研究。结果表明:本研究所选用的普通玉米自交系较糯玉米自交系的形态变异更为丰富;47个性状的Shannon-Weaver多样性指数存在较大差异。聚类分析表明,糯玉米自交系紧密地聚在一起,与普通玉米自交系存在较大形态差别。DUS测试性状涵盖的信息量大且受环境影响相对较小,可应用于玉米自交系的形态多样性分析。 相似文献
5.
Molecular characterization of global maize breeding germplasm based on genome-wide single nucleotide polymorphisms 总被引:8,自引:0,他引:8
Yanli Lu Jianbing Yan Claudia T. Guimarães Suketoshi Taba Zhuanfang Hao Shibin Gao Shaojiang Chen Jiansheng Li Shihuang Zhang Bindiganavile S. Vivek Cosmos Magorokosho Stephen Mugo Dan Makumbi Sidney N. Parentoni Trushar Shah Tingzhao Rong Jonathan H. Crouch Yunbi Xu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,120(1):93-115
Characterization of genetic diversity is of great value to assist breeders in parental line selection and breeding system design. We screened 770 maize inbred lines with 1,034 single nucleotide polymorphism (SNP) markers and identified 449 high-quality markers with no germplasm-specific biasing effects. Pairwise comparisons across three distinct sets of germplasm, CIMMYT (394), China (282), and Brazil (94), showed that the elite lines from these diverse breeding pools have been developed with only limited utilization of genetic diversity existing in the center of origin. Temperate and tropical/subtropical germplasm clearly clustered into two separate groups. The temperate germplasm could be further divided into six groups consistent with known heterotic patterns. The greatest genetic divergence was observed between temperate and tropical/subtropical lines, followed by the divergence between yellow and white kernel lines, whereas the least divergence was observed between dent and flint lines. Long-term selection for hybrid performance has contributed to significant allele differentiation between heterotic groups at 20% of the SNP loci. There appeared to be substantial levels of genetic variation between different breeding pools as revealed by missing and unique alleles. Two SNPs developed from the same candidate gene were associated with the divergence between two opposite Chinese heterotic groups. Associated allele frequency change at two SNPs and their allele missing in Brazilian germplasm indicated a linkage disequilibrium block of 142 kb. These results confirm the power of SNP markers for diversity analysis and provide a feasible approach to unique allele discovery and use in maize breeding programs. 相似文献
6.
Chuanxiao Xie Marilyn Warburton Mingshun Li Xinhai Li Muji Xiao Zhuanfang Hao Qi Zhao Shihuang Zhang 《Molecular breeding : new strategies in plant improvement》2008,21(4):407-418
This study analyzes population structure and linkage disequilibrium (LD) among 187 commonly used Chinese maize inbred lines,
representing the genetic diversity among public, commercial and historically important lines for corn breeding. Seventy SSR
loci, evenly distributed over 10 chromosomes, were assayed for polymorphism. The identified 290 alleles served to estimate
population structure and analyze the genome-wide LD. The population of lines was highly structured, showing 6 subpopulations:
BSSS (American BSSS including Reid), PA (group A germplasm derived from modern U.S. hybrids in China), PB (group B germplasm
derived from modern U.S. hybrid in China), Lan (Lancaster Surecrop), LRC (derivative lines from Lvda Reb Cob, a Chinese landrace)
and SPT (derivative lines from Si-ping-tou, a Chinese landrace). Forty lines, which formerly had an unknown and/or miscellaneous
origin and pedigree record, were assigned to the appropriate group. Relationship estimates based on SSR marker data were quantified
in a Q matrix, and this information will inform breeder’s decisions regarding crosses. Extensive inter- and intra-chromosomal
LD was detected between 70 microsatellite loci for the investigated maize lines (2109 loci pairs in LD with D′ > 0.1 and 93 out of them at P < 0.01).This suggests that rapidly evolving microsatellites may track recent population structure. Interlocus LD decay among
the diverse maize germplasm indicated that association studies in QTLs and/or candidate genes might avoid nonfunctional and
spurious associations since most of the LD blocks were broken between diverse germplasm. The defined population structure
and the LD analysis present the basis for future association mapping.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
7.
Longjiang Fan Liyan Quan Xiaodong Leng Xingyi Guo Weiming Hu Songlin Ruan Huasheng Ma Mengqian Zeng 《Molecular breeding : new strategies in plant improvement》2008,22(3):329-338
Waxy maize was first reported in China in 1909 and is mainly used in food production in Asia. The evidence for strong domestication
selection in the Waxy locus of rice and a selective sweep around its genomic region make us to wonder whether there has been similar selection
in Waxy in glutinous maize. To address this issue, DNA sequences of Waxy, three flanking genes and an unlinked gene (Adh1) of 30 accessions sampled from Chinese waxy maize accessions, including representative landraces and inbred lines, were determined
in this study. Sharp reduction of nucleotide diversity and significant neutrality tests (Tajima’s D and Fu and Li’s F*) were observed in the Waxy locus in Chinese waxy maize but not in nonglutinous maize; comparison with the unlinked gene confirmed that this pattern
was different to Waxy. Sequence analysis across a 143 kb genomic segment centered on the Waxy locus revealed patterns consistent with a selective sweep in the upstream region of Waxy. The selective sweep detected based on current limited genomic sequences exceeded over 50 kb, indicating strong selection
in this or a bigger region. However, No sweep effect was detected in the repetitive downstream region of Waxy. Phylogenetic analysis indicated that Chinese waxy maize was domesticated from the cultivated flint maize (Zea mays ssp. mays) that was introduced from the new world. At least two independent deletions in exon 7 (30 bp) and 10 (15 bp) were identified
in the Chinese accessions respectively. These findings demonstrate a similar pattern of domestication selection in the Waxy genomic region in both glutinous maize and rice, suggesting that this pattern in the rise of glutinous phenotype is likely
in other cereal crops. 相似文献
8.
Menkir A Kling JG Badu-Apraku B Ingelbrecht I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,110(6):1145-1153
Striga-resistant maize inbred lines are of interest to maize breeding programs in the savannas of Africa where the parasitic weed is endemic and causes severe yield losses in tropical maize. Assessment of the genetic diversity of such inbred lines is useful for their systematic and efficient use in a breeding program. Diversity analysis of 41 Striga-resistant maize inbred lines was conducted using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers to examine the genetic relationships among these lines and to determine the level of genetic diversity that exists within and between their source populations. The two marker systems generated 262 and 101 polymorphic fragments, respectively. Genetic similarity (GS) values among all possible pairs of inbred lines varied from 0.45 to 0.95, with a mean of 0.61±0.002 for AFLPs, and from 0.21 to 0.92, with a mean of 0.48±0.003, for SSRs. The inbred lines from each source population exhibited a broad range of GS values with the two types of markers. Both AFLPs and SSRs revealed similar levels of within population genetic variation for all source populations. Cluster and principal component analysis of GS estimates with the two markers revealed clear differentiation of the Striga-resistant inbred lines into groups according to their source populations. There was clear separation between early- and late-maturing Striga-resistant inbred lines. Considering the paucity of germplasm with good levels of resistance to Striga in maize, the broad genetic diversity detected within and among source populations demonstrates the genetic potential that exists to improve maize for resistance to Striga. 相似文献
9.
Xingxing Cai Jing Fan Zhuxi Jiang Barbara Basso Francesco Sala Alberto Spada Fabrizio Grassi Bao-Rong Lu 《PloS one》2013,8(11)
The characterization of genetic divergence and relationships of a set of germplasm is essential for its efficient applications in crop breeding and understanding of the origin/evolution of crop varieties from a given geographical region. As the largest rice producing country in Europe, Italy holds rice germplasm with abundant genetic diversity. Although Italian rice varieties and the traditional ones in particular have played important roles in rice production and breeding, knowledge concerning the origin and evolution of Italian traditional varieties is still limited. To solve the puzzle of Italian rice origin, we characterized genetic divergence and relationships of 348 rice varieties from Italy and Asia based on the polymorphisms of microsatellite fingerprints. We also included common wild rice O. rufipogon as a reference in the characterization. Results indicated relatively rich genetic diversity (H
e = 0.63-0.65) in Italian rice varieties. Further analyses revealed a close genetic relationship of the Italian traditional varieties with those from northern China, which provides strong genetic evidence for tracing the possible origin of early established rice varieties in Italy. These findings have significant implications for the rice breeding programs, in which appropriate germplasm can be selected from a given region and utilized for transferring unique genetic traits based on its genetic diversity and evolutionary relationships. 相似文献
10.
We examined the genetic diversity of 80 inbred waxy maize lines using 22 SSR molecular markers that could be used to achieve heterosis in waxy maize. Eighty inbred waxy maize lines with different phenotypes, 40 yellow, 25 white, 13 black, and two red lines were analyzed by SSR molecular marker fingerprint and cluster analysis. Using a standard genetic distance of 0.55, the 80 waxy maize inbred lines were clustered into nine groups. Among them, group II, group V, groups VII and VIII, and group IX were divided into three subgroups at a genetic distance of 0.46, into two subgroups at 0.49, into two subgroups at 0.46, and into four subgroups at 0.493, respectively. All but one of the yellow waxy maize inbred lines were clustered in groups VI, VII, VIII, and IX. Group IX (30 lines) contained 28 yellow lines; the other 11 yellow lines were distributed among groups VI, VII and VIII. Among the 25 white lines, 21 were clustered in groups III, V, VI and the third subgroup of group II. The black line N72 was in a group of its own. The black lines N75, N76 and N78 were distributed in groups VII, VIII and IX, respectively. The other nine black lines were clustered in group II. The red lines were distributed in the second subgroup of group II and there was no difference in genetic distance between them. In conclusion, there were considerable genetic differences among waxy maize inbred lines of different colors. The mean genetic distance of inbred lines of the same color was significantly less than that of lines of different colors. Therefore, we concluded that it was more accurate to determine the difference between the populations using the highly stable DNA genetic markers. 相似文献
11.
B M Prasanna 《Journal of biosciences》2012,37(5):843-855
Maize (Zea mays L.) is not only of worldwide importance as a food, feed and as a source of diverse industrially important products, but is also a model genetic organism with immense genetic diversity. Although it was first domesticated in Mexico, maize landraces are widely found across the continents. Several studies in Mexico and other countries highlighted the genetic variability in the maize germplasm. Applications of molecular markers, particularly in the last two decades, have led to new insights into the patterns of genetic diversity in maize globally, including landraces as well as wild relatives (especially teosintes) in Latin America, helping in tracking the migration routes of maize from the centers of origin, and understanding the fate of genetic diversity during maize domestication. The genome sequencing of B73 (a highly popular US Corn Belt inbred) and Palomero (a popcorn landrace in Mexico) in the recent years are important landmarks in maize research, with significant implications to our understanding of the maize genome organization and evolution. Next-generation sequencing and high-throughput genotyping platforms promise to further revolutionize our understanding of genetic diversity and for designing strategies to utilize the genomic information for maize improvement. However, the major limiting factor to exploit the genetic diversity in crops like maize is no longer genotyping, but high-throughput and precision phenotyping. There is an urgent need to establish a global phenotyping network for comprehensive and efficient characterization of maize germplasm for an array of target traits, particularly for biotic and abiotic stress tolerance and nutritional quality. ??Seeds of Discovery?? (SeeD), a novel initiative by CIMMYT with financial support from the Mexican Government for generating international public goods, has initiated intensive exploration of phenotypic and molecular diversity of maize germplasm conserved in the CIMMYT Gene Bank; this is expected to aid in effective identification and use of novel alleles and haplotypes for maize improvement. Multi-institutional efforts are required at the global level to systematically explore the maize germplasm to diversify the genetic base of elite breeding materials, create novel varieties and counter the effects of global climate changes. 相似文献
12.
Experimental evaluation of the potential of tropical germplasm for temperate maize improvement 总被引:4,自引:0,他引:4
S. P. Tallury M. M. Goodman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):54-61
Commercial maize (Zea mays L.) in the USA has a restricted genetic base as newer hybrids are largely produced from crosses among elite inbred lines
representing a small sample (predominantly about 6- to 8-base inbreds) of the Stiff stalk and Lancaster genetic backgrounds.
Thus, expansion of genetic diversity in maize has been a continuous challenge to breeders. Tropical germplasm has been viewed
as a useable source of diversity, although the integration of tropical germplasm into existing inbred line and hybrid development
is laborious. The present study is an evaluation of the potential of tropical germplasm for temperate maize improvement. All
possible single-, three-way-, and double-cross hybrids among three largely temperate and three temperate-adapted, all-tropical
inbred lines were evaluated in yield-trial tests. Single-cross hybrids containing as much as 50–60% tropical germplasm produced
8.0 t ha-1 of grain yield, equivalent to the mean yield of the commercial check hybrids. On the other hand, three-way and double-cross
hybrids with the highest mean yield contained lower amounts of tropical germplasm, 10–19% and 34–44%, respectively. Overall,
hybrids containing 10–60% tropical germplasm yielded within the range of the commercial hybrid checks. Hybrids with more than
60% tropical germplasm had significantly lower yields, and 100% tropical hybrids yielded the least among all hybrids evaluated.
The results indicate that inbred lines containing tropical germplasm are not only a useful source to expand the genetic diversity
of commercial maize hybrids, but they, also are competitive in crosses with temperate materials, producing high-yielding hybrids.
These experimental hybrids exhibited good standability (comparable to the commercial check hybrids) but contained 1–2% higher
grain moisture, leading to delayed maturity. Recurrent selection procedures are being conducted on derivatives of these materials
to extract lines with superior yield, good standability, and reduced grain moisture which can be used for commercial exploitation.
Received: 26 January 1998 / Accepted: 14 July 1998 相似文献
13.
Sherry A. Flint-Garcia Anastasia L. Bodnar M. Paul Scott 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(6):1129-1142
All crop species have been domesticated from their wild relatives, and geneticists are just now beginning to understand the consequences of artificial (human) selection on agronomic traits that are relevant today. The primary consequence is a basal loss of diversity across the genome, and an additional reduction in diversity for genes underlying traits targeted by selection. An understanding of attributes of the wild relatives may provide insight into target traits and valuable allelic variants for modern agriculture. This is especially true for maize (Zea mays ssp. mays), where its wild ancestor, teosinte (Z. mays ssp. parviglumis), is so strikingly different than modern maize. One obvious target of selection is the size and composition of the kernel. We evaluated kernel characteristics, kernel composition, and zein profiles for a diverse set of modern inbred lines, teosinte accessions, and landraces, the intermediate between inbreds and teosinte. We found that teosinte has very small seeds, but twice the protein content of landraces and inbred lines. Teosinte has a higher average alpha zein content (nearly 89% of total zeins as compared to 72% for inbred lines and 76% for landraces), and there are many novel alcohol-soluble proteins in teosinte relative to the other two germplasm groups. Nearly every zein protein varied in abundance among the germplasm groups, especially the methionine-rich delta zein protein, and the gamma zeins. Teosinte and landraces harbor phenotypic variation that will facilitate genetic dissection of kernel traits and grain quality, ultimately leading to improvement via traditional plant breeding and/or genetic engineering. 相似文献
14.
Brigitte Gouesnard Sandra Negro Amélie Laffray Jeff Glaubitz Albrecht Melchinger Pedro Revilla Jesus Moreno-Gonzalez Delphine Madur Valérie Combes Christine Tollon-Cordet Jacques Laborde Dominique Kermarrec Cyril Bauland Laurence Moreau Alain Charcosset Stéphane Nicolas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(10):2165-2189
Key message
Genotyping by sequencing is suitable for analysis of global diversity in maize. We showed the distinctiveness of flint maize inbred lines of interest to enrich the diversity of breeding programs.Abstract
Genotyping-by-sequencing (GBS) is a highly cost-effective procedure that permits the analysis of large collections of inbred lines. We used it to characterize diversity in 1191 maize flint inbred lines from the INRA collection, the European Cornfed association panel, and lines recently derived from landraces. We analyzed the properties of GBS data obtained with different imputation methods, through comparison with a 50 K SNP array. We identified seven ancestral groups within the Flint collection (dent, Northern flint, Italy, Pyrenees–Galicia, Argentina, Lacaune, Popcorn) in agreement with breeding knowledge. Analysis highlighted many crosses between different origins and the improvement of flint germplasm with dent germplasm. We performed association studies on different agronomic traits, revealing SNPs associated with cob color, kernel color, and male flowering time variation. We compared the diversity of both our collection and the USDA collection which has been previously analyzed by GBS. The population structure of the 4001 inbred lines confirmed the influence of the historical inbred lines (B73, A632, Oh43, Mo17, W182E, PH207, and Wf9) within the dent group. It showed distinctly different tropical and popcorn groups, a sweet-Northern flint group and a flint group sub-structured in Italian and European flint (Pyrenees–Galicia and Lacaune) groups. Interestingly, we identified several selective sweeps between dent, flint, and tropical inbred lines that co-localized with SNPs associated with flowering time variation. The joint analysis of collections by GBS offers opportunities for a global diversity analysis of maize inbred lines.15.
Lu H Li JS Liu JL Bernardo R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):119-126
Maize (Zea mays L.) is one of the most-important food crops in southwestern China. The diversity of maize populations from southwestern China
has been evaluated on the basis of agronomic and morphological data, but not on marker data. Our objectives were to evaluate
the allozyme polymorphism of these populations, and group the populations on the basis of allozyme data. We analyzed 27 maize
populations from southwestern China and two populations [BS13(S)C2 and Lancaster] from the USA for genetic variation at 18
allozyme loci. We found a total of 69 alleles at 18 allozyme loci with an average of 3.8 alleles per locus. Compared with
inbreds, hybrids, and populations from the U.S. Corn Belt, the 27 Chinese populations had a significantly higher (p<0.01) number of allozyme alleles per locus. Maize populations from southwestern China have accumulated abundant genetic diversity,
and might be valuable germplasm for broadening the genetic base of U.S. Corn Belt breeding germplasm. The analyses of allele-frequency
distributions and the expected heterozygosity also reflected the differences between the Chinese and the U.S. germplasm. The
Chinese populations might be valuable germplasm for complementing U.S. Corn Belt breeding germplasm. The analysis of gene
diversity showed that 77% of the allozyme variation resided within populations and 23% between populations. This result suggested
that breeders should identify one or a few Chinese populations with the best agronomic performance, and exploit the genetic
variation within these selected populations. Cluster analysis classified the 29 populations into four main groups. Groupings
based on allozyme data could be useful for classifying the populations into different heterotic groups and, consequently,
exploiting them in hybrid breeding.
Received: 12 October 2000 / Accepted: 13 March 2001 相似文献
16.
Analysis of genetic diversity in maize populations is a very important step for understanding genetic structure and subsequently
for genetic manipulations in maize breeding. Sh2, Bt2, Sh1, Wx1, Ae1 and Su1 involved in starch biosynthesis are important genes associated with yield and quality traits in maize breeding programs.
In this study, genetic diversity of these six genes in 67 Chinese elite maize inbred lines was measured using single-nucleotide
amplified polymorphisms (SNAPs). The results indicated that the number of haplotypes of each gene and population was far less
than theoretically expected 2
n
(n = the number of the SNAPs). Phenetic clustering analysis showed that the kernel phonetic (semi-) dent and (semi-) flint lines
were belong to distinct subclusters based on haplotypes of SNAPs, with a few exceptions. In addition, the genetic origin of
these maize inbred lines was associated with the clustered subgroups. Intragenic linkage disequilibrium (LD) was observed
in some of the SNAPs in Bt2, Sh1 and Ae1, while intergenic LD was observed in some of the SNAPs in Bt2, Sh1 and Su1. Association study of kernel phenotypes and SNAP haplotypes showed that the (semi-) dent and (semi-) flint lines had the
common haplotype of TA and CC at two SNAP sites in Bt2 (Bt2-2 and Bt2-5), respectively. Two haplotypes of ATGT and GTGC at four SNAP sites in Sh1 (Sh1-2, Sh1-3, Sh1-4 and Sh1-5) were associated with temperature and tropical origin of the maize inbred lines, respectively. 相似文献
17.
玉米重要自交系的肿囊腐霉茎腐病抗性鉴定与评价 总被引:5,自引:0,他引:5
由肿囊腐霉菌(Pythium inflatum Matthews)引起的玉米茎腐病是影响玉米产量的一种重要病害。为进一步拓展可利用的抗源,于2010-2011年在田间采用人工接种方法对287份重要的玉米自交系种质进行了玉米茎腐病的抗性鉴定评价。结果表明,287份鉴定材料中有171份自交系对茎腐病的抗性达到中抗以上水平,占鉴定材料的59.58%,其中高抗自交系共43份,占鉴定材料总数的14.98%;感病类型自交系共116份,占鉴定材料的40.42%,其中高感自交系共95份,占鉴定材料总数的33.10%。Lancaster、Reid及P群种质中具有丰富的茎腐病抗源,而塘四平头种质群中茎腐病抗源相对缺乏,多为感病类型。该研究结果可为今后我国玉米茎腐病抗性种质的引进和改良提供重要参考。 相似文献
18.
Fine genetic characterization of elite maize germplasm using high-throughput SNP genotyping 总被引:2,自引:0,他引:2
Xun Wu Yongxiang Li Yunsu Shi Yanchun Song Tianyu Wang Yubi Huang Yu Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(3):621-631
To investigate the genetic structure of Chinese maize germplasm, the MaizeSNP50 BeadChip with 56,110 single nucleotide polymorphisms (SNPs) was used to genotype a collection of 367 inbred lines widely used in maize breeding of China. A total of 41,819 informative SNPs with minor allele number of more than 0.05 were used to estimate the genetic diversity, relatedness, and linkage disequilibrium (LD) decay. Totally 1,015 SNPs evenly distributed in the genome were selected randomly to evaluate the population structure of these accessions. Results showed that two main groups could be determined i.e., the introduced germplasm and the local germplasm. Further, five subgroups corresponding to different heterotic groups, that is, Reid Yellow Dent (Reid), Lancaster Sure Crop (Lancaster), P group (P), Tang Sipingtou (TSPT), and Tem-tropic I group (Tem-tropic I), were determined. The genetic diversity of within subgroups was highest in the Tem-Tropic I and lowest in the P. Most lines in this panel showed limited relatedness with each other. Comparisons of gene diversity showed that there existed some conservative genetic regions in specific subgroups across the ten chromosomes, i.e., seven in the Lancaster, seven in the Reid, six in the TSPT, five in the P, and two in the Tem-Tropical I. In addition, the results also revealed that there existed fifteen conservative regions transmitted from Huangzaosi, an important foundation parent, to its descendants. These are important for further studies since the outcomes may provide clues to understand why Huangzaosi could become a foundation parent in Chinese maize breeding. For the panel of 367 elite lines, average LD distance was 391 kb and varied among different chromosomes as well as in different genomic regions of one chromosome. This analysis uncovered a high natural genetic diversity in the elite maize inbred set, suggesting that the panel can be used in association study, esp. for temperate regions. 相似文献
19.
Yang Xiao Wei Liu Yaohong Dai Cheng Fu Yinbing Bian 《World journal of microbiology & biotechnology》2010,26(3):527-536
In this study, microsatellite markers were utilized to reveal the genetic diversity of 56 strains of Lentinula edodes grown in China. A total of 224 DNA bands were detected through 25 primer pairs, of which, 223 bands (99.6%) were polymorphic between two or more strains. The variation in SSR (Simple Sequence Repeat) DNA band patterns and average genetic similarity among the wild strains of L. edodes obtained from the same region uncovered a vast genetic diversity in the natural germplasm found in China. Compared with L. edodes strains from other areas, the genetic diversity of those from the Yunnan Plateau, Hengduanshan mountains, Taiwan, and south China was significantly greater. Based on cluster analysis and principal coordinate analysis, the results indicated that all L. edodes strains could be divided into three major groups. These results effectively displayed the differences between the strains from North and South China, and those from the same or adjoining regions could cluster preferentially into small groups in most cases, suggesting the positive correlation between the cluster results and the geographical origin for the natural germplasm of Chinese L. edodes. To our knowledge, this is a pioneering report on the utilization of the SSR marker technique in analyzing L. edodes’ genetic diversity. 相似文献
20.
Patterns of genomic variation in Chinese maize inbred lines and implications for genetic improvement 总被引:2,自引:0,他引:2
Renyu Zhang Gen Xu Jiansheng Li Jianbing Yan Huihui Li Xiaohong Yang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(6):1207-1221