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1.
The complete mitochondrial genome sequence of Solen grandis that lives in sub-tidal waters and being buried in muddy to fine sand substrates, is described in this paper. The mitogenome (16,794?bp) consists of 12 protein-coding genes (loss of ATPase subunit 8), 22 tRNA genes, 2 rRNA genes and 1 putative control region. It is the typical bivalve mitochondrial gene composition. 相似文献
2.
Molluscs in general, and bivalves in particular, exhibit an extraordinary degree of mitochondrial gene order variation when
compared with other metazoans. The complete mitochondrial genome of Solen grandis (Bivalvia: Solenidae) was determined using long-PCR and genome walking techniques. The entire mitochondrial genome sequence
of S. grandis is 16,784 bp in length, and contains 36 genes including 12 protein-coding genes (atp8 is absent), 2 ribosomal RNAs, and 22 tRNAs. All genes are encoded on the same strand. Compared with other species, it bears
a novel gene order. Besides these, we find a peculiar non-coding region of 435 bp with a microsatellite-like (TA)12 element, poly-structures and many hairpin structures. In contrast to the available heterodont mitochondrial genomes from
GenBank, the complete mtDNA of S. grandis has the shortest cox3 gene, and the longest atp6, nad4, nad5 genes. 相似文献
3.
M. A. VARELA A. GONZLEZ‐TIZ
N M. FRANCISCO‐CANDEIRA A. MARTÍNEZ‐LAGE 《Molecular ecology resources》2007,7(2):221-222
Five polymorphic microsatellite loci in the razor clam Ensis siliqua are described. A collection consisting of 34 individuals from Finisterre, Spain, was analysed. Loci were isolated from the sequences of intersimple sequence repeat (ISSR) markers. Detailed analysis of 42 ISSR markers led to the design of 16 primer pairs. Five of these yielded consistent and polymorphic products. The number of alleles ranged from five to 23 per locus with the observed heterozygosity ranging from 0.46 to 0.94. Linkage equilibrium was observed in all loci and three of them showed significant deviations from Hardy–Weinberg equilibrium. 相似文献
4.
The xishishe clam Coelomactra antiquata is a commercially important bivalve species, but has been suffering from severe population decline due to over-exploitation
and the deterioration of environmental conditions. To investigate the genetic variation and structures of this clam, nine
polymorphic microsatellites were developed. The number of alleles ranged from two to 28 per locus with the observed heterozygosity
ranging from 0.083 to 0.921. This set of microsatellites will be useful for the study of the genetic structures of C. antiquata. 相似文献
5.
Freshwater mussels are among the most endangered fauna in the world, while little is known about their genetic diversity. We isolated 15 polymorphic microsatellite loci in a threatened freshwater mussel, Solenaia oleivora. The characteristics of these loci were tested with 46 individuals. The number of alleles per locus ranged from 2 to 15, and the PIC value ranged from 0.250 to 0.880. The observed and expected heterozygosity ranged from 0.167 to 0.929 and from 0.297 to 0.900, with an average of 0.562 and 0.647, respectively. Eight loci deviated significantly from Hardy–Weinberg equilibrium. These polymorphic microsatellites will be useful for the assessment of population genetic diversity and resource conservation of S. oleivora. Cross-species amplification demonstrated that six of 15 microsatellite loci amplified successfully in two other unionid species. 相似文献
6.
R. JOEL DUFF WALTER R. HOEH DOUG COOK STEPHEN C. WEEKS 《Molecular ecology resources》2004,4(3):397-399
Thirteen polymorphic microsatellite loci were isolated and characterized from the clam shrimp Eulimnadia texana. In analyses of 20–50 individuals from two populations the number of alleles ranged from two to seven with observed heterozygosity ranging between 0.00 and 0.37. The low values for heterozygosity were not unexpected for a group characterized by its unusual androdioecious mating system, in which males compete with self‐compatible hermaphrodites for offspring production. These microsatellites are likely to be useful for further evolutionary investigations of this rare mating system in these crustaceans. 相似文献
7.
The boll weevil (Anthonomus grandis Boheman) is a major insect pest of cotton in North America. Dispersal activity poses a threat to ongoing eradication efforts in the US, but little is known about the frequency of long‐distance migration. Nuclear molecular markers are needed to assess gene flow in relation to geographical distance. A biotin‐enrichment strategy was employed to develop microsatellite markers for the boll weevil. Of 23 loci isolated, 14 were polymorphic with three to 10 alleles per locus. Twelve of the polymorphic loci showed Mendelian inheritance and are likely to be useful in population genetics studies. 相似文献
8.
Isolation and characterization of microsatellite loci in Alasmidonta heterodon (Bivalvia: Unionidae)
KRISTINE M. SHAW TIM L. KING WILLIAM A. LELLIS MICHAEL S. EACKLES 《Molecular ecology resources》2006,6(2):365-367
We developed 13 species‐specific microsatellite markers for the federally endangered Atlantic slope unionid Alasmidonta heterodon. Four to 18 alleles per locus were observed among 30 individuals. Observed heterozygosity throughout the loci ranged from 26.9 to 86.2% and averaged 63.6%. Estimates of individual pairwise genetic distances indicated that levels of genetic diversity among loci were sufficient to produce unique multilocus genotypes for all animals surveyed. Randomization tests showed that genotypes for this collection were consistent with Hardy–Weinberg expectations, and no significant linkage disequilibrium was observed between loci. These loci therefore appear suitable for population surveys, kinship assessment and other such applications. 相似文献
9.
10.
Siripunkaw C Kongrit C Faries KM Monello RJ Gompper ME Eggert LS 《Molecular ecology resources》2008,8(1):199-201
We report the isolation and characterization of 17 polymorphic microsatellite loci in the North American raccoon (Procyon lotor). These loci exhibit high levels of allelic diversity, with between four and 13 alleles per locus, and heterozygosity, with observed values of 0.500-1.000 in a sample of 20 individuals. All genotypes conformed to Hardy-Weinberg expectations and there were no instances of linkage disequilibrium detected. 相似文献
11.
We identified 11 informative microsatellite loci in Tetratheca ericifolia from an (AG)n‐enriched library. Using these loci on 32 individuals from two populations (16 individuals from each), we detected an average of 11.3 alleles per locus (range of five to 21, average expected heterozygosity of 0.728), of which 56% were unique to one population or the other. All loci were amplifiable in seven to 12 of a further 12 species of Tetratheca under the same reaction conditions. The markers will be useful tools for evolutionary studies of this Australian endemic group. 相似文献
12.
Nine yellowcheek (Elopichthys bambusa) microsatellite loci were isolated using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Three
to eight alleles per locus were detected in 29 samples collected from five populations of E. bambusa. The mean number of alleles was 5.6 ± 1.9 and the level of observed heterozygosities ranged from 0.415 to 0.843. These are
the first microsatellite loci characterized from E. bambusa that can be used for estimating genetic diversity, population structure and parentage analysis. 相似文献
13.
Wilsonia backhousei is a clonal saltmarsh plant restricted to the southern latitudes of Australasia and threatened in New South Wales. We have identified eight informative microsatellite loci in the species from (AG)n‐ and (AC)n‐enriched libraries. In 48 samples from two populations we detected an average of five alleles per locus (range 2–8, average HE = 0.45), of which 72% were unique to one population or the other. Six of the eight loci were also amplifiable in Wilsonia rotundifolia under the same reaction conditions. The markers will be excellent tools for use in the management and conservation of both species. 相似文献
14.
W. J. Kim K. K. Kim J. H. Lee D. W. Park J. Y. Park J. Y. Lee 《Molecular ecology resources》2003,3(4):491-493
We described the isolation and characterization of 27 new microsatellite loci from olive flounder, Paralichthys olivaceus. All loci were found to be polymorphic, and had between five and 22 alleles with observed heterozygosity ranging from 0.161 to 1.0 in 31 individuals examined. These micorsatellite makers are likely to be useful for studies of genome mapping, mating systems and population genetics in this species. 相似文献
15.
A. Rubini F. Topini C. Riccioni F. Paolocci S. Arcioni 《Molecular ecology resources》2004,4(1):116-118
Tuber spp. are fungi that establish symbiosis with several trees and shrubs. Some of these fungal species produce edible ascomata, also known as truffles, which are highly appreciated for their taste and odour. We isolated and characterized eight polymorphic microsatellite loci from Tuber magnatum, the finest white truffle species, and assessed their variability in 370 individuals collected from all over the species range of distribution. Although two to 18 alleles per locus were found, no heterozygous individuals were observed. The availability of simple sequence repeat loci provides valuable tools for assessment of the genetic structure and population dynamics in this species. 相似文献
16.
Wei JQ Chen ZY Wang ZF Tang H Jiang YS Wei X Li XY Qi XX 《American journal of botany》2010,97(10):e89-e90
? Premise of the study: Microsatellite markers were developed in Camellia nitidissima for further population genetic studies. ? Methods and Results: Eight microsatellite markers were newly developed from C. nitidissima and 7 were transferred from other Camellia species. Two to 13 alleles per locus were identified for these microsatellites. Observed and expected heterozygosities ranged from 0.040 to 0.909, and 0.184 to 0.916, respectively. Four loci showed a significant deviation from Hardy-Weinberg equilibrium and five locus pairs displayed linkage disequilibrium. ? Conclusions: These microsatellite markers will be useful to assess the genetic variation and genetic structure of C. nitidissima. 相似文献
17.
Edward J. Heist Jennifer L. Jenkot Devon B. Keeney Ryan L. Lane Gregory R. Moyer Benjamin J. Reading Noelle L. Smith 《Molecular ecology resources》2003,3(1):59-61
We report on the development of nine polymorphic microsatellite loci in nurse shark (Ginglymostoma cirratum) using a combination of enriched and unenriched subgenomic libraries. Based on the small percentage of positive clones in the unenriched library (0.4%) it appears that microsatellites are very scarce in nurse shark genomes. Numbers of alleles at polymorphic loci ranged from two to 15; observed heterozygosity ranged from 0.17 to 0.90. We expect these loci to be useful for studies of breeding structure and paternity. 相似文献
18.
Wuchang bream (Megalobrama amblycephala) is an economically important fish in China. From a (GT)13‐enriched genomic library, 20 microsatellites were developed. Nine of these 20 loci were polymorphic in a test population with allele numbers ranging from two to four, and the observed and expected heterozygosities ranging from 0.2609 to 0.7826 and from 0.3739 to 0.7546, respectively. In the cross‐species amplifications, six of these nine loci were also polymorphic in white amur bream (Parabramis pekinensis). These polymorphic microsatellite loci are potentially useful for population genetics of Wuchang bream and its closely related species. 相似文献
19.
The pen shell Atrina pectinata is a commercially important and over-exploited bivalve. In this survey, the first set of 13 polymorphic microsatellite loci
for the pen shell was developed and characterized. The number of alleles ranged from 6 to 28 per locus with the observed and
expected heterozygosities ranging from 0.640 to 1.000 and from 0.731 to 0.970, respectively. These informative microsatellite
markers will be useful in studies of population and conservation genetics for this species. 相似文献
20.
N. ALVAREZ C. BORN A.‐M. RISTERUCCI P. SOURROUILLE B. BENREY M. HOSSAERT‐MCKEY 《Molecular ecology resources》2004,4(4):683-685
Six microsatellite loci were isolated from the bruchid Acanthoscelides obtectus Say (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from 3 to 18. We found high levels of within‐population variation at most loci, with heterozygosities ranging from 0 to 0.75. Cross‐species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obvelatus Bridwell and A. argillaceus Sharp. 相似文献