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1.
The effects of different levels of fertilization with nitrogen and potassium (NK), height within the canopy and plant age of Lycopersicon hirsutum f. glabratum (PI 134417) C. H. Mull and L. esculentum on the density of foliar cristalliferous idioblasts and trichomes, and on the levels of 2- tridecanone (2-TD) and 2-undecanone (2-UD) and their influence on the resistance to Myzus persicae (Sulzer) were investigated. NK levels were inversely related to densities of trichomes and cristalliferous idioblasts in L. esculentum, but only to densities of cristalliferous idioblasts in L. hirsutum. Trichome density increased with increasing height within the canopy and with plant age in L. esculentum and L. hirsutum. Density of cristalliferous idioblasts in L. hirsutum increased with canopy height reaching a maximum at mid-height within the canopy in opposition to L. esculentum in which a small decrease in density with height within the canopy was found. There was also a small decrease of idioblast density with increasing plant age for L. esculentum, while a greater and opposite variation took place in L. hirsutum. L. esculentum has mainly non- glandular leaf trichomes (90%), while L. hirsutum has mainly glandular trichomes (97%). The main glandular trichomes of L. hirsutum are producers of 2-TD and 2-UD, whose leaf content increases with increase of trichome density. A negative effect of 2-TD concentration and cristalliferous idioblast density on the survival and longevity of M. persicae was observed. Since L. hirsutum presented much higher levels of 2-TD and cristalliferous idioblast density than L. esculentum, L. hirsutum seems a promising source of resistance to M. persicae for tomato breeding programs.  相似文献   

2.
Putrescine uptake and translocation were studied by feeding [3H] putrescine to roots of tomato seedlings ( Lycopersicon esculentum Miller, cv. Earlypak 7) at the stage of expanded cotyledons, of maize seedlings ( Zea mais L.) at the coleoptile stage, and of one year old pines ( Pinus pinea L.). Putrescine translocation was rapid as radioactivity appeared in the upper part of the seedlings within 30 min, continuing to increase up to 24 h, while it decreased in roots. The putrescine supplied was partly metabolized to spermidine and spermine in the course of 24 h. The transport was temperature-dependent as it increased with increasing temperature from 4°C to 30°C. In plants kept in 100% relative humidity the transport decreased by 27% compared to controls kept in 50% relative humidity. The existence of basipetal transport was assessed by feeding labeled putrescine to cotyledons or to a primary leaf of tomato plants at different stages of growth. The influence of ringing at the hypocotyl level on polyamine translocation in pine plants was studied in order to exclude cortical parenchyma and phloem from transport. Radioactivity decreased in the hypocotyl just above the ring and in the upper parts (epicotyls with needles), but long-distance transport was low affected indicating xylem transport. It is suggested that polyamine transport is not polar, and that it occurs mainly through xylem vessels.  相似文献   

3.
In order to establish differences in the chilling sensitivity of domestic and wild Lycopersicon species, galactolipase (EC 3.1.1.26) activity, free fatty acid (FFA) level and Hill reaction activity were measured in chloroplasts isolated from control and cold treated leaves of L. esculentum Mill., cv. Norton, L. hirsutum Humb. and Bonpl., L. peruvianum var. glandulosum Mill. Galactolipase activity was higher in chloroplasts from Lycopersicon species with high chilling sensitivity than in chloroplasts of more chilling-resistant ones. A similar relationship was observed for FFA level in chloroplasts from both cold-stored and control leaves. Decrease in Hill reaction activity due to cold stress was greater in chloroplasts of more chilling-sensitive species. The changes are accompanied by a decline of photochemical activity. Considering the changes in the three parameters noted above, an increasing order of chilling tolerance was established: L. esculentum < L. hirsutum (700 m) < L. hirsutum (3100 m) < L. peruvianum (3400 m). It is suggested that measurements of galactolipase activity and FFA may be useful in an evaluation of differences in resistance to chilling injury of closely related species.  相似文献   

4.
Listeria monocytogenes is a food-borne pathogenic bacterium that can be found in softcheese. At the beginning of cheese ripening, the pH is about 4·85–4·90. The aimof this work was to study the influence of temperature, preincubation temperature (temperature atwhich the inoculum was cultivated) and initial bacterial concentration on the survival of L.monocytogenes (strain Scott A) at pH 4·8. It was demonstrated in an earlier study thatthese factors did influence growth kinetics. Survival studies of L. monocytogenes weredone in a laboratory broth simulating cheese composition. Four test temperatures (2, 6, 10 and14°C) and two preincubation temperatures were studied (30°C or the test temperature). Listeria monocytogenes (strain Scott A) was unable to grow at pH 4·8 under allconditions tested. The time for 10% survival was about 11 and 2 d, at 2°C with preincubationat 2°C and 30°C, respectively; 9 d at 6°C with preincubation at 6°C; 4 d at 6°Cwith preincubation at 30°C; and 1 d at 14°C with preincubation at 14°C or at 30°C.The results show that survival of L. monocytogenes (strain Scott A) at pH 4·8 is notdependent on initial bacterial concentration but on both the test and preincubation temperatures.  相似文献   

5.
By using immunolocalization and differential extraction methods we show that only apoplastic invertase, but not vacuolar invertase, was present in the mature, sucrose-accumulating L. hirsutum pericarp. In contrast, in the hexose-accumulating L. esculentum fruit, both the apoplastic and vacuolar invertase activities and protein content increase in the mature fruit. Quantitative expression studies of the soluble invertase gene (TIV1) and the apoplastic invertase genes (LINs) showed that only TIV1 gene expression could account for the species and developmental differences of both soluble and insoluble enzyme activity of the pericarp. The expression of the LIN genes encoding for apoplastic tomato invertases was unrelated to the differences in bound enzyme activity and could not account for the rise in bound invertase activity in the mature L. esculentum fruit. Evidence is presented that the bound invertase activity of tomato fruit is also the TIV1 gene product. The presence of apoplastic invertase in the mature sucrose-accumulating L. hirsutum fruit suggests a hydrolysis-resynthesis mechanism of sucrose uptake. In order to test this hypothesis, we studied short- and long-term uptakes of asymmetrically labelled 3H-fructosyl-sucrose accompanied by compartmental analysis of the sugars in attached whole fruits of L. hirsutum and L. esculentum. The results indicate that hydrolysis-resynthesis is slow in the sucrose-accumulating fruit but is not an integral part of an uptake and compartmentation mechanism.  相似文献   

6.
A chilling episode of a few hours damaged root ammonium absorption in a cultivated tomato ( Lycopersicon esculentum cv. T-5), but not in a wild congener from high altitudes ( Lycopersicon hirsutum LA1778). In the cultivar, ammonium influx was strongly temperature dependent and showed the residual effects of chilling, whereas ammonium efflux was nearly temperature invariant and showed no persistent effects. A 2 h exposure to 5 °C significantly depressed subsequent ammonium absorption at 20 °C, and about 12 h at 20 °C was required for recovery. For both the cultivated and wild species, rerooted cuttings were slightly less sensitive to chilling than seedlings. The relative inhibition (mean ± SE) of ammonium absorption before and after chilling was 58·4 ± 2·5% for the cultivated species and 29·0 ± 9·1% for the wild species. The F1 hybrid between the species showed a relative inhibition of 52·4 ± 3·6%, suggesting that chilling sensitivity may be dominant. In a backcross of the hybrid to L. esculentum , the phenotypic distribution of the relative inhibition of ammonium absorption indicated that this trait is segregating.  相似文献   

7.
Hypocotyl and root growth elongation of etiolated seedlings was measured non-destructively for the wild tomato accessions LA 460 ( Lycopersicon chilense Dun.), PI 126435, PI 127831 and PI 127832 ( L. peruvianum Mill.) and controls PI 120256 and T3 ( L. esculentum Mill.) on slant boards at 10, 15 and 20°C. Both hypocotyl and root elongation over time were fitted by a logistic growth function with three parameters estimated for each seedling by non-linear least squares regression. Analysis of variance of these equation parameters indicated linear decreases of both hypocotyl and root growth rate parameters with temperature. All four wild accessions maintained greater hypocotyl growth rate parameters at 10°C than the fast-germinating cultivated accession PI 120256, but not significantly greater than T3. Hypocotyl growth rates of the wild accessions were less inhibited at 10°C relative to 20°C than were either cultivated accession. These results suggest that these wild accessions have greater chilling tolerance than cultivated controls for early seedling growth, and may have potential use for genetically improving emergence times for tomatoes sown in cold soil.  相似文献   

8.
Cultivated tomato Lycopersicon esculentum (L.) Mill. cv. P-73 and its wild salt tolerant relative L. pennellii (Correll) D'Arcy accession PE-47, were grown during spring-summer 1989 under unheated plastic greenhouse conditions. Plants were submitted to two different salt treatments using 0 and 140 mM NaCI irrigation water. In both tomato species, salinity caused a proportionally larger reduction in leaf area than in leaf weight and, in L. esculentum , a proportionally larger decrease in stem weight than in leaf weight. Daily variations in leaf water potential (Ψ1) were fundamentally due to changes in the evaporative demand of the atmosphere. Reductions in Ψ1 due to salinity were consistent only in L. esculentum . In all the conditions studied, leaf turgor was maintained. Leaf conductance (g1)was higher in L. esculentum than in L. pennellii .Salinity induced a clear reduction in g1 levels in L. esculentum whereas, in L. pennellii , this reduction was noted only in May. In both species the Ψos (leaf osmotic potential at full turgor) levels were reduced by salinity. The bulk modulus of elasticity (E) and relative water content at turgor loss point (RWCtlp) were not affected by salinity. The RWCtlp values in L. pennellii seem to be controlled by E values.  相似文献   

9.
Mature green tomato fruit ( Lycopersicon esculentum cv. Caruso) were stored at 1°C or 20°C and analyzed on day 0, 18 and 22 for electrolyte leakage, ripening-associated changes in pigmentation and phospholipid fatty acid composition. Chilled fruit were also analyzed 4 days after they were returned to 20°C. Fruit did not ripen significantly during chilling and subsequent storage at 20°C, and showed visible chilling injury symptoms only at 20°C. Electrolyte leakage increased in control and chilled fruit, indicating enhanced membrane permeability during both ripening and chilling. Returning the fruit to ambient temperature gave an apparent decrease in electrolyte leakage. Phospholipid and linolenic acid content and double bond index decreased during ripening at 20°C. The small changes in phospholipid fatty acid composition during chilling cannot account for the enhanced membrane permeability. The significant decrease in percentage of linolenic acid and in double bond index in the total lipids, but not in the phospholipids, upon returning the fruit to 20°C suggests loss of galactolipid polyunsaturated fatty acids  相似文献   

10.
Abstract  Trichome-based host plant resistance of Lycopersicon species to potato moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), was examined in a laboratory bioassay. Neonate P. operculella were placed on the foliage of accessions of L. hirsutum , L. hirsutum f. glabratum , L. pennellii , L. cheesmanii f. minor and L. esculentum for 48 h . At the end of this period, larval mortality, the numbers of larvae emigrating from, and mining into, the leaf surface were recorded as were leaf area and the densities of trichomes. Analysis by categorical logistic regression found accessions differed in levels of resistance to P. operculella ( P  < 0.001). Further analysis using generalised linear models showed mortality was associated with high densities of type VI and low densities of type V trichomes; emigration from the leaf was associated with high densities of type I and type VII trichomes; and the numbers of mines associated with low densities of type I and type VI trichomes and increased leaf area. Although results indicate that certain accessions of L. hirsutum and L. hirsutum f. glabratum may be most appropriate for inclusion in a breeding program aimed at introducing trichome-based host plant resistance of wild Lycopersicon species into the tomato, further research is necessary to fully understand the mechanisms.  相似文献   

11.
Six inbred lines of maize ( Zea mays L.) from cool temperate regions (C) and from warm regions (W) were grown at 14, 22, 30 and 38°C up to the same physiological age, the full expansion of the third leaf. Generally, plants developed smaller shoot dry weights and leaf areas at extreme temperatures. The shoot:root ratio was lowest at 22°C and highest at 30°C. Most lines had a minimum for specific leaf dry weight at 30°C, but W lines had a second lower minimum at 14°C. Phosphofructokinase activity scarcely reacted to temperature between 22° and 38°C; at 14°C one C line and all W lines had rather low activities. Generally, the chlorophyll content increased steeply from 14 to 22°C and decreased somewhat from 30 to 38°C. In C lines the carotenoid level decreased from 14 to 38°C. No uniform temperature response was found for PEP carboxylase activity, but the highest activity was mostly attained at 38°C. RuBP carboxylase activity increased considerably from 14 to 22°C and remained comparatively constant at higher temperatures. The highest activity of NADP malate dehydrogenase was found at 22°C, with a decrease up to 38°C and with second lowest values at 14°C. C lines possessed larger leaf areas, shoot dry weights and higher shoot:root ratios than W lines at 14 and 22°C, and higher specific leaf dry weights over the whole temperature range. The genotypic pattern of shoot dry weight at 14°C corresponded reasonably well with that of phosphofructokinase activity. A better adaptation of C lines to suboptimal temperatures was mostly clearly indicated for photosynthetic traits which have a well proven relationship with the chloroplast membranes: chlorophyll, carotenoids and RuBP carboxylase. The least distinct effects of origin were observed at 38°C; a tendency prevailed for a better performance of C lines with regard to phosphofructokinase, carotenoids, RuBP carboxylase and NADP malate dehydrogenase.  相似文献   

12.
Tomato fruits are sensitive to low temperature and develop chilling injury, while at nonchilling temperatures they ripen rapidly. Previously, a hot-air treatment was found to reduce the sensitivity of the fruit to low temperatures. In the present study hot air was compared to hot water and their effects on reducing chilling injury and fungal decay were investigated. Tomatoes ( Lycopersicon esculentum cv. Daniella) at the breaker stage were subjected to hot air, 48 h at 38°C, or various hot water dips, 30 min at 40°C or 2 min at 46, 48 or 50°C, before holding at 2°C. The unheated tomatoes developed chilling injury and fungal infections at 2°C, but not at 12°C. All the heat treatments reduced chilling injury and decay in tomatoes held for 3 weeks at 2°C. The outer pericarp tissue of heated tomatoes had higher phospholipid and lower sterol contents than unheated tomatoes. Heated tomatoes also had less saturated fatty acids than unheated tomatoes held at 2°C, but not at 12°C. Scanning electron micrograph observations showed that all the fruits had microcracks in their surface, but the unheated chilled tomatoes had also fungal growth in the cracks, while those of the heated tomato fruit did not. In the areas of chilling injury collapsed cells were present under the peel and could also support pathogen development. It is suggested that the heat treatment institutes a response to high temperature stress in the fruit tissue that leads to strengthened membranes. This prevents the loss of function and cell collapse which was found in the chilling-injured areas of affected fruit.  相似文献   

13.
Abstract. A simulation of the quantitative influence of altitude on photosynthetic CO2 uptake capability (AP) included the effects of predicted changes (1) in air temperature (lapse rate) and (2) leaf temperature, (3) ambient pressure and CO2 concentration, and (4) the diffusion coefficient for CO2 in air. When a dry lapse rate (0.01°C m−1) in air temperature was simulated, significant declines (up to 14%) in AP were predicted from sea level to 4km altitude. A moist lapse rate of 0.003°C m−1 resulted in less than a 4% decrease in AP over the same altitude range. When natural leaf temperatures (predicted from heat balance analyses) were simulated, AP was significantly greater (∼20%) than when leaf temperatures were considered equal to air temperature for all lapse conditions. There was virtually no change in AP with altitude when predicted leaf temperatures and moist lapse conditions were simulated. There was a significant (∼10%) increase in AP with altitude when leaf temperature was held constant at 30°C (regardless of altitude) under moist lapse conditions. Future studies evaluating the effects of elevation on photosynthesis could benefit from the above considerations of the effects of natural leaf temperature regimes and prevailing lapse conditions on CO2 uptake potential.  相似文献   

14.
Non-grafted tomato plants ( Lycopersicon esculentum L. cv. Tmknvf2) and grafted tomato plants ( L. esculentum L. cv. Tmknvf2 ×  L. esculentum L. cv. RX-335) were grown for 30 days at three different temperatures (10°C, 25°C and 35°C). In the leaves of these plants, the enzymatic activities of SOD, GPX, CAT, APX, DHAR and GR were analysed, as were the concentrations of total H2O2, ascorbate and glutathione as well as foliar DW. Regardless of whether the plant was grafted or not, our results indicate that the thermal stress occurred mainly at 35°C, with the following effects: (1) high SOD activity; (2) H2O2 accumulation; (3) foliar-biomass reduction; (4) low GPX, CAT, APX, DHAR and GR activities; and (5) high concentrations of ascorbate and glutathione. In addition, our data show these effects to be much weaker in grafted than in non-grafted plants, directly reflected in greater biomass production. Therefore, the use of grafted plants under excessively high temperatures may offer an advantage over non-grafted plants in terms of resistance against thermal shock.  相似文献   

15.
The objective of this work was to study the effect of plant canopy height on the resistance of the tomato species Lycopersicon hirsutum to the tomato leafminer Tuta absoluta . Determination of the levels of tridecan-2-one (2-TD) and undecan-2-one (2-UD) in L. hirsutum , as well as the leaf area, density and types of trichomes present in L. hirsutum and L. esculentum were made on apical, medium and basal parts of plant canopy. Correlation of these data were made with the following biological characteristics of T. absoluta : rates of oviposition and egg hatching; length of egg, larval and pupal stages; mortality of the larval and pupal stages; pupal weight; and sex ratio. Levels of 2-TD on leaves of L. hirsutum increased from bottom to top of canopy. Tuta absoluta had higher oviposition and egg hatching, as well as lower mortalities and smaller larval and pupal periods in L. esculentum than in L. hirsutum . Longer pupal period and greater number of small and large mines of T. absoluta were verified in the apical and medium parts of L. hirsutum plants. On the other hand, higher pupal mortality and longer larval periods were obtained in the apical and basal parts of the L. hirsutum plants. Larval mortality was higher in the basal part of those plants. In L. esculentum , leaves from the apical part of the plant promoted lower mortality, longer larval period and greater number of large mines of T. absoluta . However, a shorter pupal period was verified on leaves of the basal part. It appears that an increase in glandular trichome density in L. hirsutum leads to an increase in the levels of 2-TD, which, in turn slows down larval development.  相似文献   

16.
The uptake of radioactive glucose and sucrose by protoplasts isolated from pericarp and placenta tissues of tomato ( Lycopersicon esculentum cv. Counter) fruit was investigated in relation to the dry matter accumulation rates of these tissues. Uptake of glucose by protoplasts isolated from pericarp tissue was highest in fruit of around 20 g fresh weight or 25 days after anthesis. Sucrose uptake by pericarp protoplasts was lower than that of glucose and did not show a peak of uptake. The maximum rate of glucose uptake by protoplasts from the pericarp was at the time when the tomato fruit was accumulating dry matter at the highest rate. Glucose uptake by placenta protoplasts was lower and at a similar level as sucrose.
Protoplast uptake of glucose, but not of sucrose, was partially inhibited by (1) p -chloromercuribenzene sulphonic acid, a sulphydryl group modifier; (2) erythrosin B, an H+-ATPase inhibitor; and (3) valinomycin, a K+-ionophore, suggesting that membrane transport of glucose by tomato fruit sink cells may be a carrier-mediated, energy-dependent process.
The main route of carbohydrate accumulation by tomato fruit during the period of rapid fruit growth may be by cleavage of sucrose by apoplastic acid invertase prior to hexose transport across the plasma membrane.  相似文献   

17.
Comparing heat stress effects on male-fertile and male-sterile tomatoes   总被引:13,自引:2,他引:11  
To separate the effects of heat stress on male and female reproductive tissues, male-sterile (MSs) and male-fertile tomatoes (MFs) were placed in growth chambers at 12 h day/12 h night temperatures of 28/22, 30/24 or 32/26 °C from flower appearance to seed maturation (daily mean temperatures of 25, 27 or 29 °C). Pollen from MFs was applied individually to MS flowers. As MFs were self-pollinated, heat stress was experienced by both male and female tissues. At growth temperatures of 29 °C fruit number, fruit weight per plant, and seed number per fruit were only 10%, 6·4% and 16·4%, respectively, compared with those at 25 °C. Heat stress also adversely affected fruitset in MSs, especially when experienced by donor pollen. No fruit at all developed on MSs receiving pollen produced at 29 °C, even when ovule development, pollen germination and subsequent embryo development all took place at 25 °C. Effects on fruitset in MSs were reduced if donor pollen had not experienced heat stress. MSs grown at 29 °C but receiving pollen developing at 25 °C produced 73% as much fruit (both on number and weight basis), had 40% as high fruitset and produced 87% of the seed per fruit as MSs grown at 25 °C. This use of male-sterile and male-fertile lines of tomato provides new evidence that impairment of pollen and anther development by elevated temperature will be an important contributing factor to decreased fruit set in tomato, and possibly other crops, with global warming.  相似文献   

18.
Abstract We have studied adhesion and uptake of C. trachomatis serovar E in McCoy cells under various infection conditions. Adhesion and uptake of chlamydiae was completed about 3 h after the initiation of stationary infection at 37°C, but ingestion of cell membrane-attached organisms was finished within 0.5 h at 37°C. Reincubated chlamydiae, not attached after 3 h at 37°C, attached readily to fresh McCoy cell monolayers, but to a lesser extent than the original inoculum. Our results indicate that the lack of further attachment after 3 h incubation at 37°C under stationary infection conditions has complex causes, involving both host cell and parasite. Centrifugation did not affect the uptake of chlamydiae already bound to the cell membrane, suggesting that the uptake phase of C. trachomatis serovar E by McCoy cells is unaffected by centrifugation.  相似文献   

19.
A growth chamber experiment was carried out to investigate the influence of day length and temperature on the development of flowering in eight varieties of the three grain lupin species Lupinus albus (Wat and C3396), L. angustifolius (Gungurru, Polonez and W26) and L. luteus, (Juno, Radames and Teo). The plants were grown at two temperatures, 10°C and 18°C, in combination with five daylength regimes: 10, 14, 18, 24 h day at full light intensity and 10 h full light extended with 8 h low intensity light. Increased daylength decreased days from sowing to flowering in all varieties, but had little effect on thermal time to flowering in most varieties. However, C3396, W26 and Radames had a significantly longer thermal time to flowering at high, non‐vernalising temperature (18°C) at short daylengths. Low light intensity daylength extension did not significantly influence thermal time to flowering. For flower initiation, measured as number of leaves on the main stem three types of response were found. All varieties formed fewer leaves on the main stem at 10°C than at 18°C, although the two thermo‐neutral varieties of L. luteus, Juno and Teo, gave only a small response to temperature and daylength. In Polonez, Gungurru and Wat, low temperature decreased leaf number, but there was only a small response to changes in daylength. Three varieties, C3396, W26 and Radames, showed longer thermal time to flowering at 18°C with short daylengths. This could be explained by a greater number of main stem leaves formed at short daylength at non‐vernalising temperatures. Increased daylength decreased leaf number in these varieties, but never to a smaller number than for plants grown at 10°C. In these varieties, low intensity extension of the daylength had a similar (W26, Radames) or decreased (C3396) effect compared to full light extension. The hastening of time to flowering by long days could be separated into two effects: a high light energy effect hastened development by increasing the rate of leaf appearance in all varieties, while low light energy in thermo‐sensitive varieties was able to substitute for vernalisation by decreasing leaf number.  相似文献   

20.
Plants of Lycopersicon esculentum Mill. P. I. 120262 show an increased phosphate uptake rate per unit dry weight of root after as little as one day of growth in solutions lacking phosphate. The reversibility of this response in plants experiencing various degrees of phosphate stress was investigated by measuring the depletion of phosphate from solutions over 3-h intervals. Measurements were made at three times in the first 30 h after phosphate was resupplied. Reversibility decreased as the level of phosphate stress increased. The phosphate uptake rate was returned to the level of controls after 30 h of phosphate resupply in plants grown without phosphate for one or three days. Plants grown without phosphate for five or seven days had uptake rates 26 and 40% higher than controls, respectively, after the same period of phosphate resupply. Internal phosphate concentrations after 30 h of phosphate resupply were equal to or greater than the controls in all plants. These results are consistent with a simple reversible feedback of phosphate status on phosphate transport in slightly stressed plants, but such a mechanism seems inadequate to explain the responses observed in more severely stressed plants.  相似文献   

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