Pyrosequencing™

Nucleotide sequencing is an established method for gaining information relating to partial gene, whole gene, or whole genome sequence. Here we describe some of the background leading to the advent of modern nucleotide sequencing and how it has led to the development of Pyrosequencing™, a relatively new method for real-time nucleotide sequencing. In particular, we describe how this method can be used for typing bacterial pathogens.     

PIMWalker™

This article reports on PIMWalker, a free and interactive tool for visualising protein interaction networks. PIMWalker handles the unified molecular interaction (MI) format defined by members of the Proteomics Standards Initiative (the PSI MI format), and it is thus directly and easily usable by bench biologists. PIMWalker also comes with a documented, open-source Javatrade mark application programming interface allowing the bioinformatic programmer to easily extend the functions. AVAILABILITY: PIMWalker is available under a free license from http://pim.hybrigenics.com/pimwalker.     

The ProtoChip™ immunoassay biochip

ProtoPharm makes use of the ProtoChip™, a high-density immunoassay system, to construct epitope “fingerprints” of complex protein mixtures. Unlike all other protein biochip methods, the ProtoChip does not require the immobilization of proteins or antibodies on a solid support nor does the ProtoChip require the purification of large numbers of proteins. The ProtoChip may be used to profile complex protein mixtures, identify panels of diagnostic immunoassays, identify vaccine candidates, and provide leads for antibody therapeutics.