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Rudrapatnam N. Tharanathan Akira Yokota Heike Rau Hubert Mayer 《Archives of microbiology》1993,159(5):445-452
Lipopolysaccharides (LPS), isolated from four Mycoplana species, i.e. the type strains of M. bullata, M. segnis, M. ramosa and M. dimorpha, were characterized onto their chemical composition and their respective lipid A-types. Those of M. bullata and M. segnis showed on DOC-PAGE an R-type character and had lipid A's of the Lipid ADAG-type which exclusively contained 2,3-diamino-2,3-dideoxy-d-glucose as lipid A sugar. LPS's of M. ramosa and M. dimorpha showed, although only weakly expressed, ladder-like patterns on DOC-PAGE indicating some S-type LPS's and lipid A of the d-glucosamine type (Lipid AGlcN). M. bullata LPS contained mannose and glucose in major amounts and additionally l-glycero-d-mannoheptose, whereas M. segnis LPS was composed of rhamnose, mannose and glucose together with both, d-glycero-d-manno- and l-glycero-d-manno-heptoses in a molar ratio of 1:2. All LPS's contained 2-keto-3-deoxy-octonic acid (Kdo), phosphate and an unidentified acidic component X. In addition to X, M. segnis LPS contained glucuronic and galacturonic acids, whereas M. ramosa LPS contained only galacturonic acid. Acetic acid hydrolysis of the LPS resulted in splitting off lipid A moieties, very rich in 3-hydroxy fatty acids, in particular in 3-OH-12:0 (in Lipid ADAG), or in 3-OH-14:0 (in Lipid AGlcN). Analysis of the 3-acyloxyacyl residues revealed major amounts of amide-linked 3-OH(3-OH-13:0)12:0 in lipid A of M. bullata and 3-OH(12:0)12:0 in lipid A of M. segnis. The rare 4-oxo-myristic acid (4-oxo-14:0) was observed only in M. bullata LPS, where it is ester-linked. Amide linked diesters could not be traced in M. ramosa and M. dimorpha. All four lipid A's lacked erster-bound acyloxyacyl residues.Non-standard abbreviations DAG
2,3-diamino-2,3-dideoxy-d-glucose
- Kdo
2-keto-3-deoxy-octonate
- LPS
lipopolysaccharide
- PITC
phenyl isothiocyanate
- NANA
N-acetyl neuraminic acid 相似文献
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I. S. Sergeeva I. N. Bolotov Yu. V. Bespalaya A. A. Makhrov A. L. Bukhanova V. S. Artamonova 《Biology Bulletin》2008,35(1):102-105
The shells of Pearl mussels from the basins of the Solza, Keret’, and Umba rivers flowing into the White Sea have been measured to determine the ratio of shell convexity to its maximum height. This ratio is the main character that, according to Bogatov et al. (2003), allows one to distinguish between three species of the genus Margaritifera: M. margaritifera, M. elongata, and M. borealis. It has been found that the above ratio gradually increases as the shell grows. Therefore, this character is unsuitable for species diagnosis, the more so that no hiatus in it between the three forms of pearl mussels has been revealed in any of the samples studied. On this basis, it may be concluded that Northern Europe, including Russia, is inhabited by only one species of pearl mussels, M. margaritifera. 相似文献
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《BMJ (Clinical research ed.)》1965,1(5441):1060-1062
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Comparative tissue explant requirements of Columbia-SK,C(M) and M.M. viruses,and of Theiler's encephalomyelitis virus 总被引:1,自引:0,他引:1
SHEAN DB SCHULTZ EW 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1950,73(4):622-625
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Jee WS 《Journal of musculoskeletal & neuronal interactions》2006,6(2):113-121
The author described how and when he first met Harold M. Frost, M.D., that began a journey from the Henry Ford Hospital to the University of Utah and Sun Valley Hard TissueWorkshops that sequentially developed the technology of dynamic cancellous bone histomorphometry, the ever-evolving mechanostat hypothesis and the Utah Paradigm for Bone Physiology. 相似文献
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《BMJ (Clinical research ed.)》1962,1(5286):1198-1201
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Rupert C. Barneby 《Brittonia》1985,37(1):85-87
Acacia guilandinae DC. is recognized asMimosa guilandinae (DC.) Barneby, endemic to French Guiana and adjoining Amapá, Brazil;M. pseudo-obovata is subordinated as a Brazilian var.pseudo-obovata (Taub.) Barneby to CaribbeanM. ceratonia L.; andM. laticifera is found synonymous withM. obovata Benth. Diagnostic morphological characters of each taxon are presented in key form. 相似文献
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《BMJ (Clinical research ed.)》1962,1(5287):1268-1270