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紫膜碎片的电二色性研究 总被引:1,自引:1,他引:0
悬浮在水中的嗜盐菌紫膜碎片,在外电场作用下产主定向排列.在20℃时,568nm的电二色性研究表明:外加电场为2kV/m时取向程度可达60%以上;大于5.5kV/m时,取向作用趋于饱和状态;饱和时简约电二色性为-0.437左右,视黄醛生色团的跃迁矩方向与电偶极矩方向形成60.9°夹角;紫膜的永久偶极短为9.2×10~(-24)C、M,剩余电极化率为3.0×10~(-27)m~2;紫膜的旋转扩散常数为0.53秒~(-1).曲线拟合分析表明,感应偶极对紫膜碎片的定向的贡献应予考虑.本文对紫膜碎片的定向机理进行了讨论. 相似文献
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嵌入紫膜碎片的BLM系统在不同离子强度的介质中产生不同的光电位信号。本文通过在介质中加入离子载体或离子通道剂对紫膜的光电响应信号进行了系统的研究。结果表明:上述光电位信号的差异主要是由紫膜的非质子离子的迁移引起的。与紫膜的质子跨膜迁移相比,这种非质子离子迁移也是由光驱动的,随介质的离子强度的增强而显著增强,但它的迁移速率更快,且产生反向的光电位。用通常方法所获得的光电位信号主要是这两种迁移信号的迭加。三价阳离子(La~(3+))可使紫膜质子泵反向并能抑制质子的吸收,但它似乎并不参与紫膜的非质子离子迁移。 相似文献
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以CAT12为自旋探针,用ESR自旋标记法测量了不同表面pH时酰化前后紫膜表面负电荷密度,以酰化所屏蔽的表面电荷数为标准,计算了表面pH4-11时单位菌紫质紫膜两侧的总负电荷数.结果表明:表面pH5-9时为9,表面pH≥10及表面pH < 5时增大.结果有力支持了膜表面5个二价阳离子结合位点的模型. 相似文献
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温度升高使天然紫膜在可见差分吸收光谱中650nm处出现一吸收峰,这意味着紫膜去离子兰膜的形成,而这在脱氧胆酸处理后的紫膜中并未出现.天然紫膜与脱脂紫膜在630nm处的吸收与温度间的关系进一步表明:只有天然紫膜在温度大于68℃才有膜结合离子的释放,释放的离子结合于移去的膜脂之上.紫膜结合离子的释放改变了天然紫膜中bR的构象.热致离子的释放还受介质pH值及介质中离子的影响.pH5.03时,热致离子释放温度提前至温度46℃,而pH7.00,10mM MgCl_2及pH9.00时都没有观察到紫膜离子释放.温度对紫膜在不同pH值时表面电位影响的不一致性进一步表明这是由于膜表面电荷参与的结果. 相似文献
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本文用吸收光谱和可见圆二色谱研究了不同浓度的山莨菪碱对紫膜中菌紫质结构的影响,并设计了用不同浓度的去垢剂Triton X-100作为脂环境的扰动剂,研究山莨菪碱对菌紫质的影响与膜脂关系的实验.结果表明山莨菪碱不仅影响菌紫质分子本身的构象变化而且扰动了菌紫质分子之间的激子偶联作用.通过吸收差光谱技术表明山莨菪碱对菌紫质结构的影响与膜脂密切相关并指出紫膜中菌紫质的三体结构对膜功能的贡献是不容忽视的. 相似文献
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Structural and spectroscopic characteristics of bacteriorhodopsin in air-water interface films 总被引:1,自引:0,他引:1
A suspension of purple membrane fragments in a solution of soya phosphatidyl-choline in hexane is spread at an air-water interface. Surface pressure and surface potential measurements indicate that the membrane fragments and lipids organize at the interface as an insoluble film. Electron microscopy of shadow-cast replicas of the film reveal that in the bacteriorhodopsin to soya PC weight ratio range of 2:1 to 10:1, these films consist of nonoverlapping membrane fragments which occupy approximately 35% of the surface area and are separated by a lipid monolayer. Furthermore, the membrane fragments are oriented with their intracellular surface towards the aqueous subphase. Nearly all the bacteriorhodopsin molecules at the interface are spectroscopically intact and exhibit visible spectral characteristics identical to those in aqueous suspensions of purple membrane and in intact bacteria. In addition, bacteriorhodopsin in air-dried interface films show spectral changes upon dark-adaptation and upon flash illumination similar to those observed in aqueous suspensions of purple membrane, but with slower kinetics. The kinetics of the spectral changes in interface films can be made nearly the same as in aqueous suspension by immersing the films in water. 相似文献
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Monolayer and multilayer Langmuir-Blodgett (LB) films of 6-O-alkylcelluloses with various chain lengths were prepared and studied. The surface pressure (pi)-area (A) isotherms of 6-O-alkylcelluloses exhibited characteristic behaviors depending on the length of the alkyl chain and temperature. 6-O-Stearylcellulose on the subphase formed a homogeneous monolayer at 10 mN m(-1). By transfer ratio, FT-IR, and contact angle measurements, it was proved that the monolayer of 6-O-stearylcellulose on the water surface was transferred successfully onto a substrate by a vertical dipping method to form a Z-type LB film. The transmission and reflection absorption IR spectrum indicated that the hydrocarbon chains had all-trans rotamers and were oriented nearly perpendicular to the surface in the film. AFM section analysis revealed that the thickness per layer was calculated to be 2.35 nm. These results suggested that the hydrocarbon chains were inclined at an angle of about 25.3 degrees to have high packing density in the alkyl region. 相似文献
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Antibody binding to surface antigens in membranes is the primary event in the specific immune defence of vertebrates. Here we used force microscopy to study the dynamics of antibody recognition of mutant purple membranes from Halobacterium salinarum containing a genetically appended anti-Sendai recognition epitope. Ligation of individual anti-Sendai antibodies to their antigenic epitopes was observed over time. Their increase in number within a small selected area revealed an apparent kinetic on-rate. The membrane-bound antibodies showed many different conformations that ranged from globular to V- and Y-like shapes. The maximum distance of two Fab fragments of the same antibody was observed to be approximately 18 nm, indicating an overall strong intrinsic flexibility of the antibody hinge region. Fab fragments of bound anti-Sendai antibodies were allocated to antigenic sites of the purple membrane, allowing the identification and localization of individual recognition epitopes on the surface of purple membranes. 相似文献
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In this paper, we describe the preparation and characterization of Langmuir and Langmuir-Blodgett (LB) monolayers of the enzyme organophosphorus acid anhydrolase (OPAA). Langmuir films of OPAA were characterized on different subphases, such as phosphate, ammonium carbonate, and bis-tris-propane buffers. Monolayers at the air-water interface were characterized by measuring the surface pressure and surface potential-area isotherms. In situ UV-vis absorption spectra were also recorded from the Langmuir monolayers. The enzyme activity at the air-water interface was tested by the addition of diisopropylfluorophosphate (DFP) to the subphase. LB films of OPAA were transferred to mica substrates to be studied by atomic force microscopy. Finally, a one-layer LB film of OPAA labeled with a fluorescent probe, fluorescein isothiocyanate (FITC), was deposited onto a quartz slide to be tested as sensor for DFP. The clear, pronounced response and the stability of the LB film as a DFP sensor show the potential of this system as a biosensor. 相似文献
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Protein A molecular thin film was fabricated as a platform of antibody-based biosensor. For the immobilization of the protein
A thin film, a viologen multilayer was built up using the Langmuir-Blodgett (LB) technique, and then, protein A was adsorbed
on the viologen LB film by an electrostatic interaction force, which was formed as a hetero-film structure. For the deposition
of viologen, surface pressure area (π-A) isotherm was investigated. The fabricated protein A-viologen hetero LB film was investigated
using atomic force microscopy (AFM). Using the developed molecular film, antibody immobilization and fluorescence measurement
was carried out. 相似文献
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Hidenori Nakamura Akira Tonosaki Dr. Hiroshi Washioka Keiji Takahashi Shoji Yasui 《Cell and tissue research》1985,241(3):523-528
Summary Perfusion fixation via pulmonary trunk was applied to the alveolar lining layer in situ at different lung volumes using a fixative containing tannic acid-ferrocyanide osmium. The monomolecular surface film and hypophasic tubular myelin figures were enhanced. In the range of transpulmonary pressure (1–10 cmH2O), the surface film appeared in the form of a single, electron-dense leaflet, 2.7±0.6 nm (M±SD) in thickness while trilaminar membrane structure was retained in all parts of the tubular myelin figures of the hypophase. The surface film was attached underneath at right angles with trilaminar membranes which formed the outermost parts of the tubular myelin. Such structural continuity was taken to support a view that the phospholipid unit membrane of the tubular myelin figure would be transformed at the hydrophobic phase into a pair of monomolecular leaflets, eventually forming the surface film. 相似文献