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HBIG,无环鸟苷,干扰素联合对慢性乙型肝炎抗病毒效应观察 总被引:1,自引:0,他引:1
本文报道血清HBV复制标志阳性的慢乙肝54例,随机分为治疗组及对照组各27例进行HBIG、无环鸟苷、干扰素联合近、远期抗病毒效应观察。治疗组为无环鸟苷第一周按25~20mg/kg/d计后改17~15mg/kg/d×53天,共60天;人白细胞干扰素1×106U肌注每周3次×4周,后改1.0×106U肌注每周2次×6周,共10周;HBIG400U肌注隔日1次,共10周,对照组仅给予一般“保肝”药物。其中治疗组18例,对照组19例进行治后半年到2年追踪观察,结果近、远期HBcAg、DNAP、HBV-DNA阴转率治疗组均高于对照组,其中治疗组近、远期HBcAg,HBV-DNA阴转率均达40%以上,明显高于对照组(P<0.05~0.01),治疗组近、远期各有4例及2例HBsAg阴转,而对照组则无一例阴转,从近、远期综合抗病毒效应观察,治疗组全阴率分别为33.3%、44.4%,而对照组分别为3.79%及0%,P<0.01,治疗组无明显毒副反应。对比单用无环鸟苷,全阴率31.8%;无环鸟苷加干扰素两药联合全阴率37.5%,均有所提高,达到44.4%,值得进一步研究。 相似文献
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菹草(Potamogeton crispus)对水中氮、磷的吸收及若干影响因素 总被引:24,自引:2,他引:22
菹草(Potamogeton crispus)对水中氮、磷的吸收与pH、光照、水温、根/茎生物量比及底泥间隙水与上覆水中营养盐浓度比有关。在pH为8.0-9.5、水温为19-28C的实验条件下,水中NH_4-N浓度低于0.35mg·1左右时;菹草茎、叶优先吸收NO_3-N;水中NH_4-N浓度大于0.35mg·1 ̄(-1)左右时,菹草则优先吸收NN_4-N,这一选择吸收与NH_4-N/NO_3-N比值无关。强光照、高温(30℃)和高pN的协同作用严重影响菹草硝酸盐还原酶活性。在自然条件下,菹草根部主要从底泥中吸收NH_4-N、PO_4-P,对NO_3-N吸收甚微;茎、叶主要从水层中吸收NO_3-N,对PO_4-P吸收甚少。一般情况下,浮游植物和菹草对水体中的氮、磷吸收无多大矛盾。菹草倒伏后腐败分解释放大量营养盐,为浮游植物的增殖创造了条件。 相似文献
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在家兔离体肺内动脉、脑基底动脉环观察了过氧化氢(H2O2)及次黄嘌呤+黄嘌呤氧化酶(HX+XO)对动脉环缺氧反应的影响。结果发现:(1)H2O21×10-5mol/L加强f去内皮肺动脉环(PA-)的缺氧收缩幅度(TIH),而H2O23×10-5mol/L、HX10-4mol/L+XO0.1及0.03U/ml则可抑制内皮完整肺动脉环(PA+)及PA-的TIH(P<0.05);(2)各剂量H2O2及HX+XO对有、无内皮的脑基底动脉环的TIH均无显著影响。 相似文献
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菹草(Potamogeton crispus)对水中氮、磷的吸收及若干影响因素 总被引:34,自引:0,他引:34
菹草(Potamogeton crispus)对水中氮,磷的吸收与PH,光照,水温,根/茎生物量比及底泥间隙水与上覆水中营养盐浓度比有关。在PH为8.0-9.5,水温为19-28℃的实验条件下,水中NH4-N浓度低于0.35mg.1^-1左右时,菹草茎,叶优先吸收NO3-N;水中NH4-N浓度大于0.35mg.1^-1左右时,菹草测优先吸收NH4-N这一选择吸收与NH4-N/NO3-N比值无关,强 相似文献
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在缺硼和高硼条件下,棉花的Imax明显降低,Pi的吸收受抑,高硼大于缺硼,磷由根向地上部的运输也有受抑的趋势,0、5.4、108mmol/L的硼对棉花吸收的Pi的Km值匀无明显,说明H3BO3与H2PO4^-和HPO4^=不存在竞争性抑制。 相似文献
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近年来研究表明组胺及其受体在正常造血调控中起着重要作用。本研究用琼脂半固体培养技术观察了特异性组胺H2受体激动剂英普咪定和拮抗剂西咪替丁对髓系粒-单核细胞白血病干细胞株WEHI3细胞生长的影响。结果表明不同浓度的英普咪定(10-8─10-4mol/L)对集落数呈明显剂量依赖性抑制,与对照组比较p<0.01。10-10─10-9mol/L英普咪定对集落数无明显影响。10-4─6×10-4mol/L的英普咪定对集落产率的抑制作用趋于饱和。最大抑制效能为对照组的54%(p<0.01)。10-4mol/L西咪替丁能完全阻断10-8mol/L英普咪定的集落抑制作用。对≥10-6mol/L英普咪定的作用西咪替丁均有部分阻断作用,与对照组比较P<0.01。单用西咪替丁对WEHI3细胞无明显直接作用。这提示WEHI3细胞株上存在有组胺H2受体,激动H2受体可抑制细胞增殖。 相似文献
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PCR检测中国对虾暴发性流行病毒靶基因的克隆和序列分析 总被引:2,自引:0,他引:2
An explosive epidemic disease of shrimp had occurred in China and the south Pacific coast. The causative agent is a new baculovirus called hypodermal and hematopoietic necrosis baculovirus (HHNBV) in China, and the pathogen is very much similar to Japanese panaeid rod-shaped DNA virus (PRDV) which caused mass mortality of shrimp. In order to distinguish both HHNBV and PRDV at molecular level, we have sequenced the PCR-targeted DNA fragment of HHNBV. One pair of PCR primers were prepared based on the PRDV DNA. The sequence of the PCR-targeted DNA of HHNBV is 975bp in length, and shares 99.7% homology with the PRDV. The sequence of PCR-targeted DNA of HHNBV-XIA differs from PRDV DNA in deleting three nucleotides (CAT) at 510~512 site. These characteristics of the sequence classified HHNBV and PRDV as strains of different genotypes. 相似文献
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PCR法检测对虾皮下和造血器官坏死杆状病毒 总被引:16,自引:0,他引:16
皮下和造血器官坏死杆状病毒(HHNBV)[1]、对虾杆状DNA病毒(PRDV)[2]和白斑杆状病毒(WSBV)[3]是近年来引起全球对虾暴发性死亡的病原。这三种病毒同属杆状病毒属C杆状病毒亚群中的新病毒,在流行病学和病理学特征上十分类似,因此,我们... 相似文献
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Lightner DV 《Journal of invertebrate pathology》2011,106(1):110-130
Penaeid shrimp aquaculture is an important industry in the Americas, and the industry is based almost entirely on the culture of the Pacific White Shrimp, Litopenaeus vannamei. Western Hemisphere shrimp farmers in 14 countries in 2004 produced more than 200,000 metric tons of shrimp, generated more than $2 billion in revenue, and employed more than 500,000 people. Disease has had a major impact on shrimp aquaculture in the Americas since it became a significant commercial entity in the 1970s. Diseases due to viruses, rickettsial-like bacteria, true bacteria, protozoa, and fungi have emerged as major diseases of farmed shrimp in the region. Many of the bacterial, fungal and protozoan caused diseases are managed using improved culture practices, routine sanitation, and the use of chemotherapeutics. However, the virus diseases have been far more problematic to manage and they have been responsible for the most costly epizootics. Examples include the Taura syndrome pandemic that began in 1991-1992 when the disease emerged in Ecuador, and the subsequent White Spot Disease pandemic that followed its introduction to Central America from Asia in 1999. Because of their socioeconomic significance to shrimp farming, seven of the nine crustacean diseases listed by the World Animal Organization (OIE) are virus diseases of shrimp. Of the seven virus diseases of penaeid shrimp, five are native to the Americas or have become enzootic following their introduction. The shrimp virus diseases in the Americas are increasingly being managed by exclusion using a combination of biosecurity and the practice of culturing domesticated specific pathogen-free (SPF) stocks or specific pathogen-resistant (SPR) stocks. Despite the significant challenges posed by disease, the shrimp farming industry of the Americas has responded to the challenges posed by disease and it has developed methods to manage its diseases and mature into a sustainable industry. 相似文献
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双抗体夹心ELISA法检测养殖对虾病毒的研究 总被引:3,自引:0,他引:3
用纯化的长毛对虾球状病毒(PPSV)和日本对虾中肠腺坏死杆状病毒(BMNV)制备新西兰兔抗BMNV和抗PPSV抗血清及Balb/c小鼠抗BMNV和抗PPSV抗血清,建立检测PSV和BMNV的双抗体夹心ELISA检测法,结果表明,双抗体夹心ELISA法具有较高的灵敏度,可以从100μl待测组织匀浆液中检测到50ng的PPSV蛋白,以及100ng的BMNV蛋白。不同病毒抗血清无交叉反应性,用该ELISA技术检测养殖对虾和多种采自养殖虾池及其附近的近海岸生物,发现相当比例的外观正常的对虾和近海岸生物已呈阳性反应 相似文献
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白斑综合征病毒(white spot syndrome virus,WSSV)是危害对虾的主要病原,给全球水产养殖业带来了巨大经济损失,但至今仍未发现有效的防治方法。过去10年来,国内外学者在WSSV侵染和对虾抗病毒免疫的研究方面取得了长足的进展,该文主要介绍这方面的研究进展。 相似文献
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There are five different viruses which are currently being studied for their impact on commercial farming of the black tiger prawn (Penaeus monodon) in Thailand. Some of these viruses cause disease in other penaeid shrimp species and even other crustacean species. Some occur not only in cultivated shrimp in other Asian countries, but also in those from Australia and the western hemisphere. In descending order from greatest to least economic impact on the Thai shrimp industry, the five viruses are: white-spot baculovirus, yellow-head virus, hepatopancreatic parvo-like virus, infectious hypodermal and hematopoeitic necrosis virus and monodon baculovirus. The purpose of this review is to summarize recent work on these viruses and to suggest future directions of research that may be useful in the effort to develop a sustainable shrimp industry. 相似文献
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