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1.
叙述了生物信息学中途径的研究背景;综述了近几年来相关途径及生物化学数据库及其特点;介绍了有关的途径分析方法.同时对于途径研究应用作了展望. 相似文献
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远程教育培训给生物教学带来新的生机。夯实了专业知识,梳理了生物知识,使我认识到应该如何把握生物课堂教学,提高了课堂教学设计能力,凝聚了生物教师的力量,使老师们学到了很多知识和教学技能,解决了平时教学中的一些难题,提升了生物的教学水平。 相似文献
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《小哥白尼(野生动物画报)》2011,(5)
哎呀呀,这个世界乱套了!大人变成了小孩,小孩变成了大人。现在,终于轮到各位小酷想家来当家做主了,快来看看他们都有什么出色的表现吧!看到爸妈变成了小孩,我立马学着他们的样子,下达了第一道命令:写作业去!我想他们一定会哭的,因为老师留的作业实在是太多了。新浪YOYO 相似文献
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综述了近年来乌龙茶品质的研究进展,介绍了乌龙茶的感官品质特征,阐述了乌龙茶的理化品质特征和相关药理作用,分析了影响乌龙茶品质的主要因素,概述了乌龙茶品质研究技术,提出乌龙茶品质下一步的研究重点方向。 相似文献
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亚热带丘陵茶园间作白三叶的土壤环境调控效果 总被引:15,自引:1,他引:15
在亚热带丘陵区1年生幼龄茶园通过连续4年间作白三叶大田试验,研究了其对茶园土壤环境及茶叶品质、产量的影响,结果表明,茶园间作白三叶降低了土壤容重,提高了土壤孔隙度、有机质、全氮、水解氮的含量及钾的活性,消耗了部分有效磷;增加了土壤关键层次(0~20cm)和关键时期(4~6月)的水分含量,延缓和缩短了夏季高温干旱与秋季持续干旱时间;获得了土壤降温时增温、保温与升温时降温的双向动态调控效果.增加了同一层次土壤温度的稳定性,延缓了高温和低温的出现时间,缩短了过度高温时间。从而促进了茶树生长,改善了茶叶品质,显著增加了茶叶产量。与清耕茶园相比,茶园间作白三叶后,春秋茶的酚氨比分别下降了17.10%和30.90%,产量提高了32.65%。 相似文献
7.
通过设计通用荧光PCR引物并结合DNA测序系统建立了小鼠的多重STR分型方案.实验针对小鼠基因组设计了两对不同的通用引物序列,标记了FAM荧光的通用序列和"加尾"的位点特异性引物共同用于小鼠的多重PCR的STR基因分型.本研究优化了通用引物和特异性引物间的比例,优化了多重STR-PCR的反应条件,并最终利用该技术方案实现了五重STR分型.实验验证了该方案在多重STR分型中的可行性.与传统的荧光检测PCR产物方案相比,应用通用方案完成多重PCR反应大大节省了实验时间与经费. 相似文献
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Research on the influence of sea level variations on the benthic faunas have been carried out in the Upper Ordovician of Sardinia. Study of the depositional facies and sequence analysis of the upper part (Lower Ashgill) of the Portixeddu Formation led to the identification of the sedimentary environments. Cystoids and crinoids are associated to bryozoans and brachiopods in most levels. The numerical analysis of associations and megaguilds shows that crinoids and cystoids have a higher frequency in the proximal and median facies of the upper offshore. The columnal association characterized by Conspectocrinus celticus and the coronoid Mespilocystites tregarvanicus has been discovered in the upper part of the formation. This material and complementary samples from Upper Ordovician of Sardinia and Kabylia (Algeria) bring additional data on the systematic and show the wide distribution of this fauna outside of the Ibero-armorican domain. The distribution of this echinoderm association supports a palaeogeographical position of the Ibero-armorican domain and Sardinia within the north gondwanan margin during the Lower Palaeozoic. 相似文献
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Foliar sclereids in the Magnoliaceae 总被引:4,自引:0,他引:4
SHIRLEY C. TUCKER 《Botanical journal of the Linnean Society. Linnean Society of London》1977,75(4):325-356
The foliar sclereids in 136 species representing 11 of the 12 genera of Magnoliaceae were studied and compared. Sclereids occur in four different cell assemblages within the leaf: diffuse idioblasts, mesophyll, dermal system, and the vein sheath including terminal elements. Tropical members tend to have the most highly sclerified leaves. In species of Manglietia the leaves have sclerified spongy mesophyll and either sclerified epidermis or hypodermis. In Talauma , sclerification affects the vein sheath and terminal cells of veinlets, with a specialized thick marginal vein in the Asian taxa but not in the American ones. Liriodendron and the magnolias native to the north temperate zone have only minimal sclerification, which usually is confined to the vein sheath of the midrib and the main lateral veins, and as idioblastic sclereids in the petiole and the midrib near the base of the blade. The two largest genera Magnolia and Michelia are heterogeneous and include species which vary as to their combinations of sclerified elements among the four possible types. 相似文献
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Mitsuhiro Yoneda Alfredo A. Molinolo Jerrold M. Ward Shioko Kimura Robert A. Goodlad 《Journal of visualized experiments : JoVE》2015,(104)
Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe the use of a simple device to cut and ‘roll’ mouse intestines to rapidly prepare whole mount preparations of superior and uniform quality to that which can be achieved by hand. The device comprises a base that holds 4 stainless steel rods and a top, which acts a cutting guide. The rods are inserted into the lumen of the small intestine [divided into thirds] and the colon. The rods and samples are then placed over a piece of filter paper or card into the holding slots in the base of the device. The top of the device is then positioned and serves as a cutting guide. The two angled sections in the center of the top piece are used to guide a knife or scalpel and cut the intestines longitudinally on the top of the rods. Once the intestinal sections have been cut, the top is removed and the card, tissue and rods gently removed from the device and placed on the bench. The rods are then gently rolled sideways to flatten and stick the intestinal segments onto the underlying piece of filter paper or card. The final preparation can then be examined or fixed and stored for later analysis. The preparations are invaluable for the study of intestinal changes in normal or genetically modified mouse models. The preparations have been used for the study and quantification of the effects of inflammation (colitis), damage, pre-cancerous lesions (aberrant crypt foci (ACFs) and mucin depleted foci (MDFs)) and polyps or tumors. 相似文献
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Silvestrini G Ballanti P Leopizzi M Gualtieri N Sardella D Monnazzi P Simeoni S Sebastiani M Bonucci E Patacchioli FR 《Journal of molecular histology》2007,38(3):215-226
We have studied the effects of the treatment with corticosterone (CORT), parathyroid hormone (PTH), or both (CORT + PTH),
and of their withdrawal (CORT-rec and CORT + PTH-rec), on the osteoprotegerin (OPG) and receptor activator of nuclear factor-kB
ligand (RANKL) localization and expression and on histomorphometric parameters in primary and secondary spongiosa of rat femur
and tibia metaphyses. In the secondary spongiosa of the CORT group, the bone remodeling and the OPG/RANKL mRNA ratio decreased.
In the PTH group, the bone turnover and the structural and connectivity indices increased, and the OPG/RANKL mRNA ratio fell;
this ratio rose, however, in the primary spongiosa. In the CORT + PTH group, remodeling values intermediate between those
of the CORT and PTH groups, were detected in the secondary spongiosa, where OPG and RANKL mRNA rose. Return towards control
values was found in the recovery groups. The Cartilage Growth Plate Width was reduced in the CORT and CORT + PTH groups and
returned to normal values in the recovery groups, while it was not affected by PTH. Independently of treatments, both OPG
and RANKL mRNA and proteins were co-localized in the same cartilage and bone cells and in several bone marrow cells. In conclusion,
the catabolic effects induced by CORT treatment occur together with an OPG fall and a RANKL rise. In the PTH group in which
the bone turnover increase, the OPG and RANKL mRNA expressions differ in the primary and secondary spongiosa, confirming that
the bone tissue in these sites can have different metabolic trends. 相似文献
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Environmental pollution from trace elements has been increasing in recent decades and has become an important concern for environmental agencies. The trace elements arsenic, cadmium, chromium, copper, mercury, and lead are among the elements that cause the greatest environmental impact and carry the highest risk to human health. Electrothermal atomic absorption spectrometry (ETAAS) has long been employed in trace element determination. In the last few years the main constraints of spectroscopy absorption methods have been overcome. These advances have increased the possibilities and the utility of ETAAS for trace element determination at μg L?1 levels in difficult matrices such as soils and sediments, giving greater accuracy and precision, lower economic cost, and easier sample pretreatment than other methods. The main advances come from sample manipulation for matrix destruction and preconcentration, the use of new lab-on-valve FIA systems, the solid sampling, the use of new, more efficient modifiers and in situ trapping methods for analyte stabilization and pre-concentration, and the progress in the capacity to control the atomization temperature and to correct background spectral interferences. All of them have permitted an improvement in the sensitivity, decreasing the detection limits and manipulation process, and increasing the accuracy and precision of the analyses.
Moreover, the new technology in the optic and detector systems have given rise to high-resolution continuum source ETAAS (HR-CS ETAAS) spectrometers that solve most of the constraints presented by the more conventional line source ETAAS (LS ETAAS) spectrometers. HR-CS ETAAS enables a rapid detection of several elements at once, facilitates direct determination from solid sampling, and reduces the matrix interferences and background noise. Here we give an overview of the recent advances and the different possibilities of using ETAAS, drawing on studies from the last decade on methods to analyze As, Cd, Cu, Hg, and Pb in soils and sediments. 相似文献
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种子蛋白质与蛋白质组的研究 总被引:11,自引:1,他引:10
综述了种子蛋白质与蛋白质组的研究,主要介绍了种子发育与形成、种子休眠与萌发、种子保存与活力以及种子与环境相互作用的蛋白质与蛋白质组的研究.同时阐述了当今蛋白质组学在种子研究中的应用以及所取得的成果,并展望了种子蛋白质组学的发展方向,种子生物学的研究将从基因水平走向整体水平,因此环境因子与种子蛋白质的相互作用是研究的重点.运用蛋白质组学将能揭示蛋白质的功能并明晰种子的生命机制. 相似文献
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从古至今,气候变化特别是周期性气候变化,一直深刻影响着人类社会的变革和发展,从旧石器时代人类起源迁移、新石器时代文化文明演变、历史时期王朝兴衰更替,到工业化以来社会经济发展动荡等,无不留下周期性气候变化影响的烙印。本文依据近年来古气候、古人类、环境考古等研究的新证据、新进展,从周期性气候变化的角度审视人类社会各个发展阶段、关键节点的气候特征;通过典型案例,介绍和分析旧石器、新石器、历史时期不同时空尺度周期性气候变化和人类活动之间复杂的相互作用关系,讨论自然科学和人文社会科学对气候变化与人类活动关系认识的异同,阐述在学科交叉背景下研究气候与人类活动关系的新范式。 相似文献
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In 2000 there was an oil spill at the Getúlio Vargas Refinery (REPAR/PETROBRÁS) in Paraná, Brazil. Nearly five years after contamination and the use of bioremediation, a study was carried out to identify the effects of the contaminated soil and the bioremediated soil on the germination and initial growth of Mimosa pilulifera seedlings. The experiment consisted of three treatments: petroleum-contaminated soil, bioremediated soil and uncontaminated soil, with five repetitions each. The following measurements were taken after 30, 60 and 90 days of planting: the percentage of germination, biomass and leaf area of the eophylls, biomass and length of the shoot and the roots in addition to the shoot/root ratio. The percentage of germination and the root biomass were not affected by the contaminated soil or by the bioremediated soil. On both the contaminated soil and the bioremediated soil biomass and leaf area of the eophyll were reduced. Plant length and shoot biomass were lower in the contaminated soil. Furthermore, the effect of the contaminated soil and the bioremediated soil was greater in the shoot than in the root system, since the bioremediation reduced the toxicity of the petroleum-contaminated soil. 相似文献
19.
M. Rubio P. Martínez‐Gómez A. Marais J.A. Sánchez‐Navarro V. Pallás T. Candresse 《The Annals of applied biology》2017,171(2):125-138
The stone fruit genus Prunus, within the family Rosaceae, comprises more than 230 species, some of which have great importance or value as ornamental or fruit crops. Prunus are affected by numerous viruses and viroids linked to the vegetative propagation practices in many of the cultivated species. To date, 44 viruses and three viroids have been described in the 9 main cultivated Prunus species. Seven of these viruses and one viroid have been identified in Prunus hosts within the last 5 years. This work addresses recent advances and prospects in the study of viruses and viroids affecting Prunus species, mostly concerning the detection and characterisation of the agents involved, pathogenesis analysis and the search for new control tools. New sequencing technologies are quickly reshaping the way we can identify and characterise new plant viruses and isolates. Specific efforts aimed at virus identification or data mining of high‐throughput sequencing data generated for plant genomics‐oriented purposes can efficiently reveal the presence of known or novel viruses. These technologies have also been used to gain a deeper knowledge of the pathogenesis mechanisms at the gene and miRNA expression level that underlie the interactions between Prunus spp. and their main viruses and viroids. New biotechnological control tools include the transfer of resistance by grafting, the use of new sources of resistance and the development of gene silencing strategies using genetic transformation. In addition, the application of next generation sequencing and genome editing techniques will contribute to improving our knowledge of virus–host interactions and the mechanisms of resistance. This should be of great interest in the search to obtain new Prunus cultivars capable of dealing both with known viruses and viroids and with those that are yet to be discovered in the uncertain scenario of climate change. 相似文献
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The pollen analysis of sediments of two boreholes located in the northeastern Morocco (Nador 1) and in the southeastern Spain (Andalucia G1) has allowed shedding a new light on the vegetation and climate of the North African littoral plains, the Rif Massif and the Betic Range during the Pliocene. The vegetation around the Alboran Sea was open and xeric during the Zanclean, dominated by herbs including subdesertic elements as Calligonum, Lygeum, Nitraria and Neurada. This type of vegetation indicates a dry and hot climate. The southwestern Mediterranean steppes have therefore a climatic character; they existed before the presence and the heavy pressure of Man on the environment. From the Piacenzian, the development, at Andalucia as well as at Nador, of Artemisia and the appearance of some altitude trees such as Cedrus and Cathaya indicate a vegetation change linked to a climatic change. Modifications in the vegetation observed during the same period in northwestern Mediterranean seem to indicate that the vegetation changes observed at Andalucia and Nador are controlled by the appearance of the first arctic glacial-interglacial cycles. The cedar tree appears at Nador only at the end of the Piacenzian, at time of the first cooling, while it has been found punctually in Messinian and early Pliocene sediments of Habibas in Algeria and in Messinian sediments of the Bou Regreg section at Salé in Morocco. The regular presence of Cedar at Bou Regreg during the Messinian, allows supposing that it was present in the Middle Atlas Mountains and that its development was favoured by colder conditions. 相似文献