共查询到20条相似文献,搜索用时 31 毫秒
1.
Georgina Tonarelli Javier Lottersberger Jorge L. Salvetti Saul Jacchieri Rosemeire A. Silva-Lucca Leila M. Beltramini 《Letters in Peptide Science》2000,7(4):217-224
In order to study the correlation between secondarystructure and bioaffinity of long and modified sequences ofp24 protein from HIV-1, three peptides containing the minimalsize epitope from region 208-217 (EAAEWDRVHP) were prepared.It was found that peptide p24-1n, an elongated nativesequence, has the lowest KD and a predominant-helix structure in the presence of trifluoroethanol. Three peptides containing another epitope from region 293-302 (FRDYVDRFK)were also synthesized. We have observed that modifications onthe native sequence p24-2n (region 287-308) increased the -helix content and this was correlated with an improvement of the recognition by antibodies in ELISA. 相似文献
2.
Sabrina Deroo Philippe Fournier Dietmar Theisen Nicolas H.C. Brons Hans Deckmyn Claude P. Muller 《International journal of peptide research and therapeutics》1998,5(2-3):159-162
Summary Phage displayed random-6-mer libraries were screened with a monoclonal antibody specific for a minimized ‘linear’ 7-mer epitope
of the measles virus hemagglutinin protein. No clone with the wild-type sequence was selected and most clones contained a
sequence motif not found in the wild-type sequence. Two mimotopes (LYMPQLS, SEMPQLP) were synthesized which inhibited binding
to the measles virus 95–135 times better than a wild-type peptide. Sequence comparison of proteins with known 3D-structure
indicates that the epitope corresponds to an α-helix, while the best mimotopes have no predicted helix propensity. The proline
is thought to be required for inducing a turn neccesary for mimicking part of the α-helix. The higher intrinsic stability
of such a mimotope may explain its improved binding and may be more suitable in immunogenicity experiments. 相似文献
3.
Sergei Andreev Igor Andreev Elena Nikolaeva Anna Petrukhina Vladimir Zemskov Mariam Vafina 《International journal of peptide research and therapeutics》1998,5(2-3):63-66
Summary The V3 loop from HIV-1 envelope glycoprotein gp120 is involved in viral entry and determines the cellular tropism and HIV-1-induced
cell-cell fusion. Earlier we have shown that V3 loop peptides representing the sequences of syncytia-inducing HIV strains
have high membranotropic activity. These peptides caused the lysis of liposomes of various lipid compositions, could fuse
negatively charged liposomes and induced hemolysis of erythrocytes. In contrast, peptides mimicking the sequences of non-syncytia-inducing
viruses showed no lytic or fusion activities at the same concentrations. Now we have found that the V3 loop synthetic peptides
containing the conserved GPGR region, derived from T-lymphotropic strains (BRU and MN), as opposed to peptides containing
the GPGQ region, are able to cause a pronounced membrane permeabilization (dissipation of the ΔpH and the Δψ) of human peripheral
blood lymphocytes, erythrocytes and plasma membrane vesicles at micromolar concentrations with a dose-dependent kinetics.
Analysis of the secondary structures of the peptides by circular dichroism revealed conformational changes in V3 loop peptides
depending on solvent hydrophobicity: from random coil in water to an α-helix/β-sheet conformation in trifluoroethanol. Such
structural changes of the V3 loop together with the membrane insertion of the gp41 N-terminal fusion peptide may promote the
formation of the fusion pore during virus-cell fusion. 相似文献
4.
Clippingdale Andrew B. He Wei-Lan Macris Mary Wade John D. Barrow Colin J. 《International journal of peptide research and therapeutics》1999,6(5-6):289-293
Summary Modifications to secondary structure and fibril formation caused by multiple acetylHmb backbone amide protection of Alzheimer's
disease Aβ(1–40) were investigated using circular dichroism spectroscopy and electron microscopy. Penta(acetylHmb) Aβ(1–40)
was observed to have a reduced ability to form α-helix and β-sheet structures under the same solution conditions as the native
peptide, with α-helical propensity being reduced more significantly than β-sheet propensity. Further, acetylHmb backbone protection
was found to alter Aβ(1–40) interaction with SDS-micelles by preventing α-helix formation. Aβ fibril formation, a characteristic
property of this peptide, was also not observed for penta(acetylHmb) Aβ(1–40). 相似文献
5.
The members of the PKA regulatory subunit family (PKA-R family) were analyzed by multiple sequence alignment and clustering
based on phylogenetic tree construction. According to the phylogenetic trees generated from multiple sequence alignment of
the complete sequences, the PKA-R family was divided into four subfamilies (types I to IV). Members of each subfamily were
exclusively from animals (types I and II), fungi (type III), and alveolates (type IV). Application of the same methodology
to the cAMP-binding domains, and subsequently to the region delimited by β-strands 6 and 7 of the crystal structures of bovine
RIα and rat RIIβ (the phosphate-binding cassette; PBC), proved that this highly conserved region was enough to classify unequivocally
the members of the PKA-R family. A single signature sequence, F–G–E–[LIV]–A–L–[LIMV]–x(3)–[PV]–R–[ANQV]–A, corresponding to
the PBC was identified which is characteristic of the PKA-R family and is sufficient to distinguish it from other members
of the cyclic nucleotide-binding protein superfamily. Specific determinants for the A and B domains of each R-subunit type
were also identified. Conserved residues defining the signature motif are important for interaction with cAMP or for positioning
the residues that directly interact with cAMP. Conversely, residues that define subfamilies or domain types are not conserved
and are mostly located on the loop that connects α-helix B′ and β strand 7.
Received: 2 November 2000/Accepted: 14 June 2001 相似文献
6.
Tam James P. Dong Xiang Chao Wu Cui Rong 《International journal of peptide research and therapeutics》1999,6(5-6):265-273
Summary This paper describes a convenient oxidative refolding method that exploits the dual property of dimethylsulfoxide (DMSO) as
an oxidant and a solvent ‘chaperon’ in assisting disulfide formation in peptides. Synthetic peptides with preferred secondary
structures were used as models. For β-sheet peptides, an active fragment of fibroblast growth factor containing the tetrapeptide
Arg-Ser-Arg-Arg at a reverse turn was used. A disulfide scan consisting of 16 analogs is designed in which different pairs
of amino acids on the antiparallel β-sheets flanking the active determinants are replaced with cysteine. DMSO in aqueous buffer
at pH 6 or 8 was found to minimize aggregation due to β-sheet formation in all 16 β-stranded peptides and provided monocyclic
disulfide peptides within 7 h. In contrast, air oxidation required a significantly longer duration for completion under similar
conditions, and only 8 of 16 peptides formed intramolecular disulfides. Rate accelerations at pH 8 were found in exo-disulfide
formation involving peptides with amino terminal cysteine, irrespective of oxidation conditions. The exo-disulfide effect
in accelerating disulfide formation may be useful for regiospecific disulfide formation. For α-helix peptides, the twodisulfide
endothelin (ET) was used as a model. DMSO in combination with trifluoroethanol (TFE) was found to favor the desired bicyclic
1,4-disulfide bridged ET (1,4-ET) over the incorrectly folded 1,3-ET. Under aqueous conditions at pH 5–11, 1,4-ET to 1,3-ET
was formed in the ratio of approximately 3:1, while the use of DMSO and TFE increased the ratio to 11:1. This solvent combination
may stabilize an α-helical stretch found in ET and contribute to enhanced selectivity. Thus, our results show that DMSO in
disulfide formation in an aqueous or helix-promoting solution may serve as an oxidant and a ‘chaperon’ solvent system to provide
regiospecificity for oxidative disulfide formation. 相似文献
7.
Transgenic technologies provide a promising means by which desirable traits can be introduced into cultured fish species within
a single generation thus accelerating the production of genetically superior broodstock for aquaculture. However, before such
fish are allowed to be marketed as food they must receive government regulatory approval. Two pivotal regulatory requirements
are: (1) complete characterization of the genomically integrated transgene and, (2) demonstration that the transgene remains
stable over multiple generations. We have generated a stable line of growth hormone (GH) transgenic Atlantic salmon (Salmo salar) using an “all fish” gene construct (opAFP-GHc2) containing a growth hormone cDNA from chinook salmon whose expression is
regulated by the 5′ promoter and 3′ termination regions derived from an ocean pout antifreeze protein (AFP) gene. In this
study we show that a reorganized form of the opAFP-GHc2 construct (termed EO-1α) integrated as a single functional copy into
a 35 bp repeat region of the genomic DNA. PCR based mapping revealed that the linear sequence of the EO-1α integrant was organized
as follows: base pairs 1580–2193 of the ocean pout promoter region followed by the intact chinook salmon GH cDNA, the complete
ocean pout antifreeze 3′ region, and the first 1678 bp of the ocean pout antifreeze 5′ region. Sequence analysis of the EO-1α
integrant and genomic flanking regions in F2 and F4 generation salmon revealed that they were identical. In addition, apart from the disruption at the integration sites, the
consensus sequences of the integrant in these two generations of salmon were identical to the sequence of the opAFP-GHc2 construct.
These results indicate that the EO-1α transgene codes for the chinook salmon GH, and that the transgene and the integration
site have remained stable over multiple generations. 相似文献
8.
Mauricio Urquiza Johanna Melo-Cardenas Tatiana Guevara Ignacia Echeverria Isabel C. Rodriguez Magnolia Vanegas Mario Amzel Manuel E. Patarroyo 《Amino acids》2010,39(5):1507-1519
We tested the hypothesis that stabilizing α-helix of Epstein–Barr virus gH-derived peptide 11438 used for binding human cells
will increase its biological activity. Non-stable α-helix of peptide 11438 was unfolded in an entropy-driven process, despite
the opposing effect of the enthalpy factor. Adding and/or changing amino acids in peptide 11438 allowed the designing of peptides
33207, 33208 and 33210; peptides 33208 and 33210 displayed higher helical content due to a decreased unfolding entropy change
as was determined by AGADIR, molecular dynamics and circular dichroism analysis. Peptides 33207, 33208 and 33210 inhibited
EBV invasion of peripheral blood mononuclear cells and displayed epitopes more similar to native protein than peptide 11438;
these peptides could be useful for detecting antibodies induced by native gH protein since they displayed high reactivity
with anti-EBV antibodies. Anti-peptide 33207 antibodies showed higher reactivity with EBV than anti-peptide 11438 antibodies
being useful for inducing antibodies against EBV. Anti-peptide 33210 antibodies inhibit EBV invasion of epithelial cells better
than anti-peptide 11438 antibodies. Peptide 33210 bound to normal T lymphocytes and Raji cells stronger than peptide 11438
and also induced apoptosis of monocytes and Raji cells but not of normal T cells in a similar way to EBV-gH. Peptide 33210
inhibited the monocytes’ development toward dendritic cells better than EBV and peptide 11438. In conclusion, stabilizing
the α-helix in peptides 33208 and 33210 designed from peptide 11438 increased the antigenicity and the ability of the antibodies
induced by peptides of inhibiting EBV invasion of host cells. 相似文献
9.
V. D. Lazareva N. E. Bocharova K. E. Volynski T. M. Volkova E. V. Grishin 《Russian Journal of Bioorganic Chemistry》2000,26(4):271-276
α-Latrotoxin (α-LTX) binding sites to functionally active monoclonal antibodies (MA) A4 and A24 were localized using three
approaches: hydrolysis of the toxin followed by theN-terminal sequencing of immunoreactive peptides; the study of antibody interaction with several recombinant α-LTX fragments;
Western immunoblotting of synthetic overlapping peptides (6–8 aa) whose structures correspond to that of the immunoreactive
α-LTX fragment. It was shown that the MA A4 epitope is located within the F234-M294 protein fragment and that MA A24 interacts with the fragment347FDKDIT352. 相似文献
10.
Jasionowski Marek Łankiewicz Leszek Wiczk Wiesław Gwizdała Elwira Grzonka Zbigniew 《International journal of peptide research and therapeutics》1998,5(5-6):371-374
Summary Conformational features of the neuropeptide pituitary adenylate cyclase activating polypeptide (1–27) (PACAP(1–27)) and its
shorter fragments (1–5), (7–11) and (14–27) were studied by circular dichroism (CD) and fluorescence spectroscopy. The obtained
CD spectra revealed that only PACAP(1–27) and the fragment (14–27) possess some content of an organized structure — the α-helix.
This C-terminal, helical part of the peptides is important for receptor binding as it provides a stable structure that can
reside in the ordered lipid region of the receptor site in the membrane, while the primary biological function of the hormone
resides in the N-terminal, disordered part. Fluorescence studies have revealed that the tyrosine residue located in the helical
region of PACAP has a higher quantum yield and a longer average lifetime than the tyrosine in the N-terminus, probably due
to a ‘shielding’ effect of the hydrophobic cluster around Tyr22. 相似文献
11.
Peter R. Andreana Przemyslaw Kowal Adam J. Janczuk Peng George Wang 《Glycoconjugate journal》2003,20(2):107-118
Galactose oxidase (EC 1.1.3.9, GAO) was used to convert the C-6′ OH of Galβ(1 → 4)Glcβ–OBn (5) to the corresponding hydrated
aldehyde (7). Chemical modification, through dehydratative coupling and reductive amination, gave rise to a small library
of Galβ(1 → 4)Glcβ–OBn analogues (9a–f, 10, 11). UDP-[6-3H]Gal studies indicated that α1,3-galactosyltransferase recognized the C-6′ modified Galβ(1 → 4)Glcβ–OBn analogues (9a–f,
10, 11). Preparative scale reactions ensued, utilizing a single enzyme UDP-Gal conversion as well as a dual enzymatic system
(GalE and α1,3GalT), taking full advantage of the more economical UDP-Glc, giving rise to compounds 6, 15–22. Galα(1 → 3)Galβ(1
→ 4)Glcβ–OBn trisaccharide (6) was produced on a large scale (2 g) and subjected to the same chemoenzymatic modification as
stated above to produce C-6″ modified derivatives (23–30). An ELISA bioassay was performed utilizing human anti-αGal antibodies
to study the binding affinity of the derivatized epitopes (6, 15–30). Modifications made at the C-6′ position did not alter
the IgG antibody's ability to recognize the unnatural epitopes. Modifications made at the C-6″ position resulted in significant
or complete abrogation of recognition. The results indicate that the C-6′ OH of the αGal trisaccharide epitope is not mandatory
for antibody recognition. Published in 2004.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
12.
The genes of the heavy and light chain variable region (VH, VL) of Z12 antibody against hTNF-α were cloned, and according
to the translated sequence of amino acids, the spatial structures of VH and VL domains were modeled by using homology-based
modeling method, followed by constructing the whole three-dimensional structure of Fv fragment. The complex model of Fv interacting
with hTNF-α was gained with computer-guided molecular docking method, based on which, it was predicted that the epitope recognized
by Z12 was from 141 to 146 of hTNF-α. hTNF-α molecule was divided into two fragments of N-terminal region from 1 to 91 and
C-terminal region from 92 to 157 with prokaryotic expression. The measured results suggested that the antigenic epitope recognized
by Z12 antibody was located in the C-terminal region 92–157 of hTNF-α, proving the predicted result reliable preliminarily.
Further experimental results showed that after hTNF-α 141–146 residues were deleted, Z12 antibody almost lost the ability
to recognize the mutant, suggesting that the amino acid residues from 141 to 146 of hTNF-α were specially recognized by Z12
antibody. 相似文献
13.
Zinovia Spyranti Theodore Tselios George Deraos John Matsoukas Georgios A. Spyroulias 《Amino acids》2010,38(3):929-936
Myelin basic protein peptide 83–99 (MBP83–99) is the most immunodominant epitope playing a significant role in the multiple
sclerosis (MS), an autoimmune disease of the central nervous system. Many peptide analogues, linear or cyclic have been designed
and synthesized based on this segment in order to inhibit the experimental autoimmune encephalomyelitis, the best well-known
animal model of MS. In this study, the solution structural motif of MBP83–99 has been performed using 2D 1H-NMR spectroscopy in dimethyl sulfoxide. A rather extended conformation, along with the formation of a well defined α-helix
spanning residues Val87–Phe90 is proposed, as no long-range NOE are presented. Moreover, the residues of MBP peptide that are important for T-cell receptor
recognition are solvent exposed. The spatial arrangement of the side chain all over the sequence of our NMR based model exhibits
great similarity with the solid state model, while both TCR contacts occupy the same region in space. 相似文献
14.
ON Rogacheva BF Shchegolev VE Stefanov GA Zakharov EV Savvateeva-Popova 《Biochemistry. Biokhimii?a》2012,77(5):456-464
Protein-ligand docking and molecular dynamics studies have shown that the key event initiated by 3′:5′-AMP binding to the
A- and B-domains of protein kinase A Iα regulatory subunit is formation of a hydrogen bond between 3′:5′-AMP and A202(A326)
(the residue in parentheses being from the B-domain). The A202(A326) amide group movement associated with the bond formation
leads to reorganization of the phosphate binding cassette (PBC) (the short 310-helix becomes the long α-helix). This process results in L203(L327) displacement and finally causes hinge (B-helix) rotation.
The L203(L327) displacement and packing into the hydrophobic pocket formed by the PBC and β2β3-loop also depends on the β2β3-loop
conformation. The correct conformation is maintained by R, I, E, but not K at position 209(333) of the A- and B-domains. So,
the R209K and R333K mutants have problems with reaching B-conformation. The apo-form of the 3′:5′-AMP-binding domain also
undergoes transition from H- to B-conformation. In this case, the movement of A202(A326) amide group seems to be a result
of reorganization of the PBC into a more stable α-helix. 相似文献
15.
Carolin Heggemann Carsten Budke Benjamin Schomburg Zsuzsa Majer Marco Wißbrock Thomas Koop Norbert Sewald 《Amino acids》2010,38(1):213-222
Antifreeze glycoproteins enable life at temperatures below the freezing point of physiological solutions. They usually consist
of the repetitive tripeptide unit (-Ala-Ala-Thr-) with the disaccharide α-d-galactosyl-(1–3)-β-N-acetyl-d-galactosamine attached to each hydroxyl group of threonine. Monoglycosylated analogues have been synthesized from the corresponding
monoglycosylated threonine building block by microwave-assisted solid phase peptide synthesis. This method allows the preparation
of analogues containing sequence variations which are not accessible by other synthetic methods. As antifreeze glycoproteins
consist of numerous isoforms they are difficult to obtain in pure form from natural sources. The synthetic peptides have been
structurally analyzed by CD and NMR spectroscopy in proton exchange experiments revealing a structure as flexible as reported
for the native peptides. Microphysical recrystallization tests show an ice structuring influence and ice growth inhibition
depending on the concentration, chain length and sequence of the peptides. 相似文献
16.
Induction of human cytotoxic T lymphocytes that preferentially recognise tumour cells bearing a conformational p53 mutant 总被引:1,自引:0,他引:1
McArdle SE Rees RC Mulcahy KA Saba J McIntyre CA Murray AK 《Cancer immunology, immunotherapy : CII》2000,49(8):417-425
The tumour-suppressor gene p53 is pivotal in the regulation of apoptosis, and point mutations within p53 are the commonest genetic alterations in human cancers. Cytotoxic T lymphocytes (CTL) recognise peptide-MHC complexes on
the surface of tumour cells and bring about lysis. Therefore, p53-derived peptides are potential candidates for immunisation
strategies designed to induce antitumour CTL in patients. Conformational changes in the p53 protein, generated as a result
of point mutations, frequently expose the 240 epitope, RHSVV (amino acids 212–217), which may be processed differently from
the wild-type protein resulting in an altered MHC-associated peptide repertoire recognised by tumour-specific CTL. In this
study 42 peptides (37 overlapping nonameric peptides, from amino acids 193–237 and peptides 186–194, 187–197, 188–197, 263–272,
264–272, possessing binding motifs for HLA-A2) derived from the wild-type p53 protein sequence were assayed for their ability
to stabilise HLA-A2 molecules in MHC class I stabilisation assays. Of the peptides tested, 24 stabilised HLA-A2 molecules
with high affinity (fluorescence ratio>1.5) at 26 °C, and five (187–197, 193–200, 217–224, 263–272 and 264–272) also stabilised
the complexes at 37 °C. Peptides 188–197, 196–203 and 217–225 have not previously been identified as binders of HLA-A2 molecules
and, of these, peptide 217–225 stabilised HLA-A2 molecules with the highest fluorescence ratio. Peptide 217–225 was chosen
to generate HLA-A2-restricted CTL in vitro; peptide 264–272 was used as a positive control. The two primary CTL thus generated
(CTL-217 using peptide 217–225; and CTL-264 using peptide 264–272) were capable of specifically killing peptide-pulsed T2
or JY cells. In order to determine whether these peptides were endogenously processed and to test the hypothesis that mutants
expressing different protein conformations would generate an alternative peptide repertoire at the cell surface, a panel of
target cells was generated. HLA-A2+ SaOs-2 cells were transfected with p53 cDNA containing point mutations at either position 175 (R → H) or 273 (R → H) (SaOs-2/175
and SaOs-2/273). Two HLA-A2-negative cell lines, A431 and SKBr3, naturally expressing p53 mutations at positions 273 and 175
respectively, were transfected with a cDNA encoding HLA-A2. The results showed that primary CTL generated in response to both
peptides were capable of killing SaOs-2/175 and SKBr3-A2 cells, which possess the same mutation, but not SaOs-2/273, A431-A2
or SKBr3 cells transfected with control vector. This suggests that these peptides are presented on the surface of SaOs-2/175
and SKBr3-A2 cells in a conformation-dependent manner and represent potentially useful target peptides for immunotherapy.
Received: 23 March 2000 / Accepted: 22 June 2000 相似文献
17.
Andrea Caporale Mattia Sturlese Elisabetta Schievano Stefano Mammi Evaristo Peggion 《Amino acids》2010,39(5):1369-1379
The N-terminal 1–34 fragment of parathyroid hormone (PTH) is fully active in vitro and in vivo and it can reproduce all biological
responses characteristic of the native intact PTH. Recently, analogues of PTH(1–11) fragments with helicity-enhancing substitutions
have been demonstrated to yield potent analogues of PTH(1–34). The work describes the synthesis, biological activity and structure
of analogues of the best modified PTH sequence H-Aib-Val-Aib-Glu-Ile-Gln-Leu-Nle-His-Gln-Har-NH2 (I). In particular, the effect of the Ala/Aib substitution at positions 1 and 3 as well as of the replacement of Nle in position
8 with d-Nle, l-(αMe)-Nle and d-(αMe)-Nle was studied. The resulting peptides were characterized structurally by CD spectroscopy, solution NMR and MD, and
in vitro for activity with respect to the cognate receptor, parathyroid hormone receptor. 相似文献
18.
T. Aerts J. Clauwaert P. Haezebrouck E. Peeters H. Van Dael 《European biophysics journal : EBJ》1997,25(5-6):445-454
We have studied the quaternary structure of α-crystallin in the presence of increasing concentrations of amphiphilic and neutral detergents using gel filtration, light-scattering,
boundary and equilibrium sedimentation. We observed a continuous reduction of the molar mass of the polymeric α-crystallin on increasing the concentration of sodium dodecyl sulphate from 0.1 mM to 5 mM, ending up with the monomeric peptides.
Dodecyltrimethylammonium bromide also disrupts the oligomeric structure of α-crystallin but the interaction appears to be cooperative: in the sharp transition region (for a 1 mg/ml protein solution)
from 3 to 8 mM of the detergent, only the native protein and a mixture of monomeric and dimeric peptide-DTAB complexes can
be observed. Concomitant studies of the circular dichroism in the far UV revealed a substantial decrease of the β-sheet and increase of the α-helix secondary structure. The latter can be related to the presence of amphiphilic polypeptide sequences in the constituent
αA and αB peptides. These studies reveal for the first time a direct relation between changes in the secondary structure of the αA and αB peptides and the formation of the oligomeric α-crystallin structure: the binding of the amphiphilic detergent reduces the β-sheet content, induces the formation of α-helix secondary structure and reduces the tendency of the peptide to form large aggregates. The different mechanisms for
reducing the oligomeric size by anionic and cationic detergents with identical apolar parts stresses the importance of charge
interactions. Our findings support some aspects of the micelle model of α-crystallin and can be related to its chaperone activity.
Accepted: 18 October 1996 相似文献
19.
The anti-IgG properties of two dual-specific (anti-dsDNA and anti-IgG) monoclonal NZB/NZW F1-derived autoantibodies, BV 17–45
and BV 16–13, were studied to resolve the location and possible commonality of the IgG epitope. To determine if BV 17–45 and
BV 16–13 recognized the same IgG epitope, the relative temperature sensitivity of the conformational IgG epitopes were evaluated
using the conformational sensitive immunoassay. Comparison of the temperature sensitivity of the conformational immunoglobulin
epitopes over a temperature range of 25–100°C suggested that the epitope recognized by BV 17–45 was the same as the IgG epitope
recognized by BV 16–13. Further studies with papain- and pepsin-generated F(ab′)2, Fab, and Fc fragments of BV 17–45 and BV 16–13 revealed that the dual-specific autoantibodies BV 17–45 and BV 16–13 both
bound an epitope in the hinge region of the IgG molecule. The potential correlation between these studies and the pathogenic
nature of dual-specific autoantibodies is discussed. 相似文献
20.
The anti-IgG properties of two dual-specific (anti-dsDNA and anti-IgG) monoclonal NZB/NZW F1-derived autoantibodies, BV 17–45
and BV 16–13, were studied to resolve the location and possible commonality of the IgG epitope. To determine if BV 17–45 and
BV 16–13 recognized the same IgG epitope, the relative temperature sensitivity of the conformational IgG epitopes were evaluated
using the conformational sensitive immunoassay. Comparison of the temperature sensitivity of the conformational immunoglobulin
epitopes over a temperature range of 25–100°C suggested that the epitope recognized by BV 17–45 was the same as the IgG epitope
recognized by BV 16–13. Further studies with papain- and pepsin-generated F(ab′)2, Fab, and Fc fragments of BV 17–45 and BV 16–13 revealed that the dual-specific autoantibodies BV 17–45 and BV 16–13 both
bound an epitope in the hinge region of the IgG molecule. The potential correlation between these studies and the pathogenic
nature of dual-specific autoantibodies is discussed. 相似文献