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1.
Under anaerobic conditions in the light, active K influx in Hydrodictyon africanum is supported by cyclic photophosphorylation.The use of selective inhibitors shows that, in the presenceof CO 2, a considerable portion of the ATP used by the K pumpis supplied by noncyclic photophosphorylation. The rest of theATP in these conditions comes from cyclic photophosphorylation.This is true under light-limiting as well as light-saturatedconditions. If non-cyclic photophosphorylation is inhibited (by removalof carbon dioxide, by the addition of cyanide which interfereswith the carboxylation reaction, or by inhibition of photosystemtwo with DCMU or supplying only far-red light), the K influxat low light intensities is stimulated, and its characteristicsbecome those of a process powered by cyclic photophosphorylationalone. These results are interpreted in terms of a competitionfor ATP between K influx and CO 2 fixation. Implicit in thisexplanation is a requirement for a switch of excitation energyabsorbed by photosystem one from cyclic photophosphorylationto non-cyclic photophosphorylation whenever conditions (presenceof CO 2and photosystem two activity) allow CO 2 fixation to occur. Further evidence for such a switch of excitation energy absorbedby photosystem one was obtained in experiments in which redand far-red light were applied separately and together. It wasfound that CO 2 fixation showed the Emerson enhancement effect,while K influx (in the presence of CO 2) shows a de-enhancement.This suggests that far-red light alone powers cyclic photophosphorylation;if red light is also present, some of the far-red quanta arediverted to non-cyclic photophosphorylation. The nature of the interaction between cyclic and non-cyclicphotophosphorylation is discussed in relation to these and otherpublished results. 相似文献
2.
The phytochrome mediated enhancement of peroxidase activityin maize leaves was repressed by inhibitors of cytoplasmic proteinsynthesis, whereas inhibitors of RNA and organelle protein synthesiswere ineffective. Continuous far-red light had no effect onthe DNA level in the leaves, but it increased the RNA levelafter a lag of 2 hr. Under continuous far-red light the totalcontent of polyribosomes also increased after a lag of 2 hr.Isolated polyribosomes from far-red grown plants showed an enhancedrate of the in vitro incorporation of amino acids into proteinsas compared to dark grown plants. These results indicate thatthe phytochrome regulation of peroxidase activity occurs atthe translational level.
1Present address: School of Life Sciences, University of Hyderabad,Hyderabad-500001, India (Received July 25, 1979; ) 相似文献
3.
Both the initial and the terminal 1 hr portions of the subjectiveday fraction, namely the L1- arid L2-phases, of a 24 hr daymust be illuminated in order for the day to be perceived asa long day in the min-LD determination by the long-day plant, Lemna gibba G3 ( 9). The light requirement of the L1-phase wassatisfied by a 10 min red light pulse given at the beginningof the phase. The red light effect was erased by a subsequent10 min far-red light, indicating phytochrome-mediated processesoccurring in the L1-phase. The light requirement of the L2-phasewas satisfied by blue or far-red light given during the terminal10 min period of the phase; there was no indication of phytochromeinvolvement. The light action on the L1-phase was replaced by10 5 M of cyclic AMP or 10 7 M of DL-isoproterenol.The isoproterenol action was antagonized by 10 7 M ofDL-propranolol. Cyclic AMP (10 5 M) combined with salicylicacid (10 6 M), which can remove the light requirementof the L2-phase ( 10), rendered a completely dark day physiologicallyequivalent to a long day. Acetylcholine (10 5 M) exertednyctomimetic action on the L1-phase of the second light day.The action of acetylcholine was antagonized by cyclic AMP (10 5M). The L2-phase required no light in the presence of 10 7M of DL-propranolol, and this propranolol action was not affectedby isoproterenol. These findings suggest changes in membranepermeability caused by the light given during the L1- and L2-phases. (Received July 7, 1976; ) 相似文献
4.
Photophosphorylation of spinach chloroplasts was uncoupled bypreincubation at 0°C in the presence of a neutral salt atpH 6.0 to 6.5 ("cold-anion uncoupling"). Preincubation at 20°Ccaused some depression in both photophosphorylation and theHill reaction, but the efficiency of photophosphorylation wasnot depressed much. Low pH values accelerated uncoupling. Theeffectiveness of anions tested as sodium salts in inducing uncouplingwas of the order: SCN->>NO 3>Cl >SO 42There was little difference in effectiveness among monovalentcations; LiCl, NaCl, KCl, RbCl and CsCl. 10 4M ATP orADP largely protected chloroplasts from cold-anion uncoupling.Addition of EDTA-extract or dicyclohexylcarbodiimide to uncoupledchloroplasts partially restored photophosphorylation. Theseobservations suggest that inactivation of chloroplast ATPaseis one cause of cold-anion uncoupling. At low light intensities, the time lag and the depression ofthe efficiency of photophosphorylation were more pronouncedin cold-anion uncoupled chloroplasts than in the control chloroplasts. (Received February 15, 1972; ) 相似文献
5.
Chloroplast development was followed in intact bean leaves illuminatedwith far-red light by extracting chloroplasts at various timesto assay photosynthetic activities. Photochemical activity wasdetected in isolated chloroplasts prior to the times which werepreviously reported for intact leaf discs. Cyclic phosphorylationwas observed in isolated chloroplasts after 8 h of far-red illuminationwhile non-cyclic electron transport and phosphorylation weremeasurable after 12 and 16 h of illumination respectively. TheP/2e ratios were less than 0.5 after 24 h of far-red exposurebut approached a value of 1.0 by 60 h of illumination. Ammoniumchloride (10 3 M) had little effect on electron transportin isolated chloroplasts until after 24 h of far-red illumination.Chlorophyll a accumulated slowly from the onset of far-red illuminationwhile chlorophyll b was not detected until after 48 h of far-redexposure. Leaf fresh weight increased four-fold over the 60h illumination period. Electron microscopy of isolated chloroplasts from far-red-illuminatedleaves indicated the presence of unfused primary thylakoidsby 12 h of exposure and prolamellar bodies throughout the entire60 h illumination period. Grana were not observed in isolatedchloroplasts nor were they induced by a 2 min exposure of thechloroplasts to 172 000 lx of white light. O 2 evolution in leaf discs of far-red-illuminated plants wasmeasurable after 16 h of illumination, attained a maximum valueby 36 h of far-red exposure, and then declined. Net CO 2 fixationwas observed in leaf discs after 8 h of far-red illuminationand the rates remained constant for an additional 16 h, beforeincreasing at least two-fold. 相似文献
6.
Developed pine seedlings synthesize chlorophyll in darkness. Their photosystem II reducing capacity is very low. The development of chloroplast structure and of photophosphorylation ability has been studied in plastids isolated from Pinus nigra (var. austriaca) developed for 14 days under different spectral compositions of light as compared with chloroplasts isolated from seedlings grown under white light or in darkness. Chloroplast structure was studied by electron microscopy. Cyclic and non-cyclic photophosphorylation were studied under white light. The spectral bands which are efficient for the development of granal structures are different from those needed to make photosystem II functional: red light makes photosystem II functional but does not permit the formation of granal structure, and under yellow light the granal structure develops but photosystem II is not fully functional. Orange light alone fulfils both these purposes. The spectral band around 650 nm seems effective in making the photosystem II functional. 相似文献
7.
Levels of ferricyanide reduction, cyclic and non-cyclic photophosphorylation were measured in chloroplasts of two cultivars
of pea and a comparison of their P/2e + ratios were made. No differences were observed in cyclic photophosphorylation or ferricyanide reduction but non-cyclic photophosphorylation
was lower in chloroplasts from the dwarf than the normal cultivar. Thus the P/2e + ratio of the dwarf was lower than the normal. Dwarf seedlings treated with gibberellic acid (GA 3) had similar rates of cyclic photophosphorylation as the untreated dwarf but non-cyclic photophosphorylation was lower as
was ferricyanide reduction. This resulted in P/2e + ratios that were higher in chloroplasts from the GA 3 treated dwarf seedlings than the untreated, and were the same as the untreated normal. Addition of GA 3 directly to the chloroplasts did not alter the activity in any way. Hence gibberellins do not directly affect changes in
chloroplastic activity but may conceivably be involved in a feed-back control system. 相似文献
8.
Based on the observations on the time lag and the low efficiencyof photophosphorylation at low light intensities, a two-stepexcitation kinetics of photophosphorylation of chloroplastswas developed, which could explain some of the hitherto unexplainedfacts concerning photophosphorylation. By analyses of the experimental results along the line of thetheory, it was inferred that the assumed substance (X) responsiblefor ATP formation occurs in a relatively large quantity of about1 mole/520 moles of chlorophyll. It was concluded thatthe high energy intermediate (X **) of ATP formation which wasassumed to accumulate on pre-illuminating the chloroplasts (inthe absence of ADP and Pi) does not represent a side pool ofextra energy, but a substantial intermediate in the normal courseof ATP formation. Alternative possibilities for the explanation of the experimentalfacts were also discussed.
1This paper is submitted to the University of Tokyo to fulfilla part of the requirements for the doctorate of H. SAKURAI. 相似文献
9.
1. Photophosphorylation was measured with spinach grana sampleswashed by 0.8 M Tris buffer at pH 8.0, which no longer catalyzedthe ferricyanide and NADP HILL reactions with water as the electrondonor. The photophosphorylation with the reaction mixture containing2 10 4 M 2,6-dichlorophenol indophenol (DCPI) plus above2 10 3 M ascorbate as the electron donor system insteadof water under anaerobic conditions was, in the most part, dependenton the addition of both PPNR (a nonheme iron protein requisitefor photosynthetic pyridine nucleotide reduction ; spinach ferredoxin)and NADP as the electron acceptor system. However, when ascorbateconcentration only was lowered to 2 10 4 M, the entirephotophosphorylation proceeded, even in the absence of the electronacceptor system. 2. When the NADP added in the reaction mixture had been reducedby glucose-6-phosphate and glucose-6-phosphate dehydrogenasebefore illumination, the photophosphorylation with 2 10 4M DCPI plus 6.7 10 3 M ascorbate decreased to aboutthe same rate as that obtained without NADP. 3. The time course for photophosphorylation in the presenceof NADP was consistent with the time course for the photoreductionof NADP: On the complete reduction of NADP, the photophosphorylationstopped. 4. In the presence of 6.7 10 3 M dichloropheny 1.1,1-dimethylureaor 3 10 4 M o-phenanthroline, non-cyclic photophosphorylationwith 2 10 4 M DCPI plus 6.7 10 3 M ascorbateas the electron donor system decreased to about half that ofthe control, and the remaining activities were hardly affectedeven at higher concentrations of both inhibitors. The P/2e ratios of non-cyclic photophosphorylation in the absence andpresence of ophenanthroline were 0.74 and 0.48, respectively.
1Present address: Department of Biology, University of California,San Diego, La Jolla, California 92037, U. S. A. 相似文献
10.
In non-growing two-celled protonemata of Adiantum capillus-veneris,apical growth was induced most effectively by red light irradiation;half of the samples were induced to grow by 660 nm light ofca. 1.5 J m 2 and the maximum number by ca. 70 J m 2.The reciprocity law was valid in this photoinduction. The growthresumption became detectable 6 hr after the light irradiationand reached a plateau within 24 hr irrespective of given fluences.When non-growing samples were irradiated with red light of 4.6W m 2 for 4 sec or shorter, the effect was fully reversedby a subsequent irradiation with far-red light to the far-redlight control level. But, when the red light was given for 16sec or longer, photoreversibility became partial. An interveningdark period of 2 min between red and far-red light did not significantlyinfluence the photoreversibility so that the escape reactionin the dark may not be attributed to the above-mentioned lossof photoreversibility. By means of a local irradiation with a narrow red light beam(10 µm in width), the apical cell was found to be photosensitivefor the growth induction, but basal cell was not. Photoreceptivesite was not localized in any particular region of the apicalcell, but was rather dispersed in the entire apical cell. (Received January 26, 1981; Accepted March 10, 1981) 相似文献
11.
Tentoxin and, to a lesser extent, dihydrotentoxin (both at 10mmol m 3) reduce stomatal opening in epidermal stripsof Commelina communis in the light but not in darkness. Thiseffect was significantly greater in normal air than in CO 2-freeair. Fusicoccin overcame the tentoxin effect. However, tentoxindid not inhibit stomatal opening in the light in epidermal stripsof Paphiopedilum harrisianum, a species which lacks guard cellchloroplasts. It is concluded that tentoxin exerts its actionon stomata not by an ionophorous effect in the plasmalemma ofguard cells but by the inhibition of photophosphorylation intheir chloroplasts. The effects of DCMU and tentoxin on guardcells are discussed in terms of their effects on chloroplastsand the extent to which energy is supplied from this organelleduring stomatal opening in the light. The results indicate thatneither photophosphorylation nor non-cyclic electron transportin guard cell chloroplasts are essential for stomatal opening. Key words: Commelina, epidermal strips, Paphiopedilum, photophosphorylation, stomata, tentoxin 相似文献
12.
1. The rate of O 2-uptake of Lemna gibba G 3 changed with a dampeddiurnal rhythm under continuous illumination given after shortdays. The rhythm was started by a light-on stimulus with a 6hr lag period and is thought to be under the control of a biologicalclock. 2. The 6 hr lag period was replaceable with a 6 hr dark periodinterrupted twice (at 0 and 3 hr) by a brief illumination withred light. The effect of red light was removed by immediateexposure to far-red light. This effect of far-red was reversedby subsequent red light. The 6 hr lag may involve a phytochrome-mediatedreaction which may be preparatory to the induction of this rhythm. (Received December 13, 1969; ) 相似文献
13.
Summary A simplified extraction procedure was used to study the effect of red and far-red light (683 and 712 nm) on the incorporation of 32P and on the distribution pattern of various phosphate fractions in 8 min-experiments under nitrogen. Nitrogen was used to avoid competition for energy-rich phosphate bonds between CO 2-fixation and the formation of polyphosphates. The total incorporation of 32P was higher in red than in far-red light, and so was the percentage of the acidsoluble organic phosphate. In the absence of oxygen the incorporation in the dark was rather low and mainly confined to orthophosphate. The ratio between the amount of labelling of polyphosphates and that of acid-soluble organic phosphates was higher in far-red light and in the dark than in red light.DCMU, even in a nitrogen atmosphere, produced a servre inhibition in red light. This inhibition increased with increasing light intensity. The labelling of organic phosphates was more affected than that of polyphosphates, while orthophosphate incorporation was least inhibited. In far-red light, DCMU exerted little influence except at a rather high light intensity, showing that cyclic photophosphorylation was proceeding alone.Antimycin A, on the other hand, was almost ineffective in strong red light, but produced a serious inhibition in far-red light. In red light of medium intensity, antimycin effected some inhibition, although much less than DCMU. Under these conditions the effect of the two inhibitors was additive when they were applied together. Labelling of polyphosphates was more sensitive to antimycin A than labelling of acid-soluble organic phosphates.It may be concluded from the data presented that far-red light produces conditions for pure cyclic photophosphorylation, whereas a large proportion of the photophosphorylation taking place in red light in the absence of CO 2 and exogenous oxygen might be regarded as pseudocyclic. The distribution pattern of the phosphorylated fractions under the different conditions suggests that polyphosphate formation in the light is favoured but not exclusively effected by cyclic photophosphorylation.
Herrn Prof. Dr. F. Overbeck zum 70. Geburtstag gewidmet. 相似文献
14.
1. Cyclic photophosphorylation driven by white light, as followed by 14CO 2 fixation by mesophyll chloroplast preparations of the C 4 plant Digitaria sanguinalis, was specifically inhibited by disalicylidenepropanediamine (DSPD), antimycin A, 2,5-dibromo-3-methyl-6-isopropyl- p-benzoquinone (DBMIb), 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide (EDAC), and KCN suggesting that ferredoxin, cytochrome b563, plastoquinone, cytochrome f, and plastocyanin are obligatory intermediates of cyclic electron flow. It was found that 0.2 μM DCMU and 40 μM o-phenanthroline blocked noncyclic electron flow, stimulated cyclic photophosphorylation, and caused a partial reversal (40–100%) of the inhibition by DBMIB and antimycin A, but not DSPD. 2. Cyclic photophosphorylation could also be activated using only far-red illumination. Under this condition, however, cyclic photophosphorylation was much less sensitive to the inhibitors DBMIB, EDAC and antimycin A, but remained completely sensitive to DSPD and KCN. Inhibition in far-red light was not increased by preincubating the chloroplasts with the various inhibitors for several minutes in white light. 3. The striking correspondence between the effects of photosystem II inhibitors, DCMU and o-phenanthroline, on cyclic photophosphorylation under white light and cyclic photophosphorylation under far-red light (in the absence of photosystem II inhibitors) suggests that electrons flowing from photosystem II may regulate the pathway of cyclic electron flow. 相似文献
15.
Irradiation of spores of the fern Mohria caffrorum Sw. witheither red light (67.4 µW cm 2) or far-red light(63.2 µW cm 2) for a period of 24 h induced maximumlevels of germination. Brief irradiations with blue light (127.6µW cm 2) administered before or after photoinductioncompletely nullified the effects of red or far-red light; however,with prolonged exposure to blue light, germination levels roseto near maximum. The similar effects of red and far-red lightin promoting spore germination makes the involvement of phytochromein this process questionable. Based on energy requirements,the promotive and inhibitory phases of blue light appear toinvolve independent modes of action. Mohria caffrorum, ferns, spore germination, photoinduction, phytochrome 相似文献
16.
Intact chloroplasts (about 70% Class I chloroplasts) isolatedfrom spinach leaves incorporated 150 nmoles of [1- 14C] acetateinto fatty acids per mg chlorophyll in 1 hr at pH 8.3, 25°Cand 25,000 lux. On electron and phase-contrast microscopiescombined with hypotonic treatment of chloroplasts, this syntheticactivity was shown to be proportional to the percentage of ClassI chloroplasts in the preparation. Light was necessary for thesynthesis, the activity in the complete reaction mixture inthe dark being only 2% of that in the light. The synthetic activityincreased with increasing intensities of light to reach saturationat 6,000 lux. CoA and ATP were most effective as cofactors,HCO 3, HPO 42, Mg 2$ and Mn 2$ were less effective.ATP could be replaced by ADP in the presence of Pi, suggestingpossible supply of ATP by photophosphorylation. Omission ofthe NADPH-generation system and NADH did not affect the synthesis,indicating sufficient provision of endogenous NADPH and NADHin intact chloroplasts under light. Addition of DTE did notcause recovery of the synthetic activity of intact chloroplastsin the dark.
1 Present address: Radioisotope Centre, University of Tokyo,Yayoi, Bunkyo, Tokyo 113, Japan. (Received August 26, 1974; ) 相似文献
17.
Localization of redox active substance(s) in chloroplast envelopeswas revealed by means of the oxidation of Cyt c by isolatedouter and inner envelope preparations. Irradiated chloroplastsreduced extra-chloroplastic Cyt c probably by an envelope electrontransfer chain. The rate was saturated at a level of about 10µmol (mg Chl) 1 h 1 under weak light of 10µEm 2 s 1. Cyt c photoreduction was inhibited by DCMUbut not by 2,5-dibromo-3-methyl-6-isopropyl- p-benzoquinone (DBMIB)indicating that the plastoquinone site is the junction of photosyntheticelectron transfer chain to envelope redox substance. Completesuppression of the non-cyclic photophosphorylation of thylakoidsby the presence of envelope membranes indicates that there isan alternative electron transfer path-way in envelope membranesthat bypasses over the pH-forming plastoquinone shuttle in thephotosynthetic electron transfer chain.
1 Present address: Photosynthesis Research Laboratory, Instituteof Physical and Chemical Research (RIKEN), Wako, Saitama, 351-0198Japan. 相似文献
18.
The effects of DBMIB on photophosphorylation and glycolysis in Scenedesmus obtusiusculus Chod. were investigated by measuring the uptake of inorganic phosphate. To analyze the effects of DBMIB on the different energy coupling possibilities in open chain and cyclic photophosphorylation, DBMIB was given to the algae in narrow concentration intervals between 10 ?6M to 10 ?4M, either alone, or in combination with DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) or desaspidin. DBMIB inhibits non-cyclic as well as cyclic photophosphorylation in Scenedesmus. However, the DCMU resistant photophosphorylation reactions are less sensitive to DBMIB than the open chain photophosphorylating system in non-DCMU treated cells. Low concentrations of DBMIB even released a part of the DCMU inhibition. Experiments with combinations of DBMIB and desaspidin also indicated that cyclic photophosphorylation is less sensitive to DBMIB than non-cyclic. The inhibition of DCMU resistant cyclic phosphorylation by DBMIB, which is a competitive inhibitor of quinones, indicated a participation of plastoquinones in this type of energy coupling as well as in the non-cyclic and DCMU-sensitive processes. The cyclic and the non-cyclic photophosphorylation pathways probably use different parts of the plastoquinone pool. For the purpose of the experiments, it was necessary to produce data for the effect of DBMIB (10 ?6–10 ?4M) on glycolysis. The highest concentration gave 50% inhibition. 相似文献
19.
Optimal cyclic photophosphorylation with reduced indophenols under anaerobic conditions was shown to require a critical redox balance. Over-reduction inhibited this phosphorylation; addition of oxidizing agents like ferricyanide, air, ferredoxin or ferredoxin plus triphosphopyridine nucleotide relieved the inhibition. When ascorbate and indophenol served as the electron donor couple for TPN+ reduction, only the amount of TPNH formed was dependent on the concentration of TPN+. The phosphorylation observed in this system was dependent only on the concentration of indophenol, and on the ability of reduced indophenol to mediate cyclic photophosphorylation. The cyclic electron flow with reduced indophenol was shown to operate simultaneously with the non-cyclic electron flow to TPN+. It was concluded that there was no phosphorylation site in the non-cyclic electron flow between ascorbate-indophenol and TPN+ and that the phosphorylation observed in this case was due only to cyclic photophosphorylation with the reduced indophenols. In the light of these results, a working hypothesis with two different sites for cyclic and non-cyclic photophosphorylation is suggested. 相似文献
20.
Laminar pulvini of bean ( Phaseolus vulgaris L.) contain numerouschloroplasts in cells of their motor tissue. The quantitativerelationships of the chloroplast pigments, chlorophyll a and b, ß-carotene, lutein, neoxanthin as well as the xanthophyllcycle carotenoids (violaxanthin, antheraxanthin and zeaxanthin)were similar to those of mesophyll chloroplasts from leafletlaminae. Exposure of pulvinules to light caused deepoxidationof violaxanthin to zeaxanthin, showing that the xanthophyllcycle is functioning. Chlorophyll fluorescence analysis of pulvinulesconfirmed that their chloroplasts are capable of both photosyntheticelectron transport and non-photochemical fluorescence quenching,showing that they build up a considerable transthylakoid protongradient in the light. Application of DCMU to excised pulvinulesand laminar discs, as well as to pulvinules of intact, attachedterminal leaflets blocked electron transport and fluorescencequenching. Application of the uncoupler CCCP to intact pulvinulesalso prevented non-photochemical fluorescence quenching. Therate of movement of the low-light-adapted terminal leaflet inresponse to exposure of its pulvinule to overhead red light(500 µmol m 2 s 1) was reduced when the pulvinulewas pretreated with DCMU. The pulvinar response to overhead blue light (50 µmol 2 s 1), which is morepronounced than to red light, was not affected by similar pretreatmentwith DCMU. Pretreatment with CCCP caused a short lag in theresponse to red light, but did not affect its subsequent rate.The results suggest that the pulvinar response to red, but notto blue light, requires non-cyclic electron transport and theresulting generation of ATP Key words: Leaf movements, light, non-cyclic electron transport, Phaseolus, pulvinar chloroplasts 相似文献
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