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1.
在宁夏六盘山香水河小流域,选择建立了4种典型森林的样地,测定了2011年生长季(5月24日—10月20日)大气降水(724.3 mm)、穿透水、干流、枯落物渗透水和主根系层(0—30 cm)土壤渗透水的无机氮(NH~+_4-N、NO~-_3-N)浓度及相应通量的变化。结果表明,在林外降水转化林下降水中,各样地林下降水携带的生长季NH~+_4-N通量(kg/hm~2)(华北落叶松人工林2.40、华山松次生林2.37、野李子灌丛2.29、桦木次生林2.09)均明显低于林外降水(3.04),NO~-_3-N通量(kg/hm~2)(华北落叶松人工林2.15、桦木次生林2.14、野李子灌丛2.09、华山松次生林1.92)更显著低于林外降水(4.27)。整体看来,阔叶林林冠的无机氮吸附(吸收)作用稍高于针叶林。在4种森林样地的枯落物层渗透水中,无机氮浓度变化在0.68—0.88 mg/L,稍高于林冠穿透水的无机氮浓度;4个样地的枯落物渗透水的无机氮通量(kg/hm~2)(野李子灌丛4.10、桦木次生林3.24、华山松次生林3.22、华北落叶松人工林2.77)均低于林下降水。在华山松次生林和华北落叶松人工林的主根系层(0—30 cm)土壤渗透水中,无机氮浓度均高于枯落物渗漏水;因土壤淋出作用,华山松次生林和华北落叶松人工林的土壤渗透水的无机氮输出通量分别为16.34 kg/hm~2和18.93 kg/hm~2,均显著高于枯落物渗透水的输入通量。整体来看,林外降水在林地无机氮输入中占有重要地位,在研究年份生长季,林冠层和枯落物层的吸附(吸收)作用使降水输入的无机氮通量明显降低,但主根系层土壤淋出作用显著增大了土壤渗透水携带的无机氮输出通量,表现为土壤层氮素流失。  相似文献   

2.
赤红壤植蔗坡地坡面径流及溶解态氮磷流失特征   总被引:4,自引:0,他引:4  
为探究南方高强度、高频次降雨下赤红壤区坡耕地土壤侵蚀及氮磷养分流失的特征,基于野外径流小区原位观测试验,通过测定自然降雨下赤红壤植蔗坡地坡面径流和溶解态氮磷流失量,探讨自然降雨下甘蔗种植对赤红壤坡面径流及溶解态氮磷流失的影响。结果表明:(1)2019年和2020年,径流量分别为1111.3 m~3/hm~2和3269.4 m~3/hm~2,硝态氮(NO~-3-N)流失量分别为1.39 kg/hm~2和15.60 kg/hm~2,铵态氮(NH~+4-N)流失量分别为0.37 kg/hm~2和1.02 kg/hm~2,可溶性磷流失量分别为0.20 kg/hm~2和0.27 kg/hm~2。2019年和2020年植蔗坡地径流及溶解态氮磷流失量均集中在6月份,占流失总量的45%以上,硝态氮(NO~-3-N)是径流氮素流失的主要形式,占79%以上。此外,2019年和2020年5月至8月,侵蚀性降雨场次分别为18次和23次,侵蚀性降雨量分别为407.8 mm和668.0 mm。(2)不同侵蚀性降雨条件下,植蔗坡地溶解态氮磷流失量及其...  相似文献   

3.
In semi-arid grassland ecosystems, soil biogeochemical processes are controlled by seasonal and inter-annual rainfall variation and temperature, which may override the long-term impact of grazers on N availability and N dynamics. In a three-year (2004?C2006) case study of an Inner Mongolian grassland, we analysed time-integrated (ion-exchange resins) and instantaneous (soil mineral N extractions) inorganic N availability at three sites of varying grazing intensities and combined these data with information on soil water content (SWC), aboveground net primary productivity (ANPP) and plant N uptake. Additionally, the effects of rainfall and grazing on N-form availability (NO 3 ? -N, NH 4 + -N) were considered. Grazing had less impact on N availability compared to seasonal and annual rainfall distribution. One of the three study years (2004) showed a grazing effect with higher resin-N availability at the ungrazed site compared to the heavily grazed site. Inorganic N availability was low in the driest year (2005) and highest in a year of average rainfall amount and favourable distribution (2004). In general, we found a positive relationship between inorganic N availability and both plant productivity and plant N uptake. Rainfall also controlled the plant available NO 3 ? -N and NH 4 + -N pools; NH 4 + -N dominated the available inorganic N-form in times of low SWC, while the available NO 3 ? -N increased with SWC. We observed N availability and plant productivity in a temporal synchronized pattern. Increased rainfall variability and land-use practices affecting SWC will likely alter N availability dynamics (and the relation of N-forms) and, therefore, important processes of semi-arid natural grassland carbon and N cycling.  相似文献   

4.
安娟  郑粉莉  李桂芳  王彬 《生态学报》2011,31(24):7579-7590
目前,土壤水分饱和和壤中流条件下,雨滴打击对养分流失的影响尚不清楚.通过3个近地表土壤水文条件(自由入渗、土壤水分饱和与壤中流)下,土槽上方架设与不架设尼龙纱网模拟降雨对比试验,研究雨滴打击对黑土坡面侵蚀过程及NO3-N、NH4-N与PO4-P随径流和侵蚀泥沙迁移的影响.结果表明,纱网覆盖消除雨滴打击后坡面侵蚀量和泥沙浓度分别减少59.4%-71.6%和57.3% -73.0%,不同水文条件下减少量的排序为:自由入渗>壤中流>土壤水分饱和.消除雨滴打击后养分随径流流失的减少仅在自由入渗条件下体现较明显,该水文条件下NO3-N、NH4-N与PO4-P流失分别减少33.3%、23.1%和40.7%;3种水文条件下,消除雨滴打击均明显减少养分随泥沙的流失,其中自由人渗条件下减少效果最明显,该水文条件下,NO3-N和NH4-N流失分别减少20.9% -54.9%和25.0% -62.3%,而PO4-P流失减少在74.6%以上.雨滴打击增大了NO3-N的淋失,但对NH4-N与PO4-P的淋失几乎无影响.消除雨滴打击后,自由人渗条件下养分的等效径流迁移深度减少26.7%-42.6%,而土壤水分饱和与壤中流条件下基本无变化.以上研究结果为有效防治坡面土壤侵蚀和农业非点源污染提供科学理论依据,尤其是在壤中流出现的地方.  相似文献   

5.

Background and aims

Climate warming, nitrogen (N) deposition and land use change are some of the drivers affecting ecosystem processes such as soil carbon (C) and N dynamics, yet the interactive effects of those drivers on ecosystem processes are poorly understood. This study aimed to understand mechanisms of interactive effects of temperature, form of N deposition and land use type on soil C and N mineralization.

Methods

We studied, in a laboratory incubation experiment, the effects of temperature (15 vs. 25 °C) and species of N deposition (NH4 +-N vs. NO3 ?-N) on soil CO2 efflux, dissolved organic C (DOC) and N (DON), NH4 +-N, and NO3 ?-N concentrations using intact soil columns collected from adjacent forest and grassland ecosystems in north-central Alberta.

Results

Temperature and land use type interacted to affect soil CO2 efflux, concentrations of DON, NH4 +-N and NO3 ?-N in most measurement times, with the higher incubation temperature resulted in the higher CO2 efflux and NH4 +-N concentrations in forest soils and higher DON and NO3 ?-N concentrations in grassland soils. Temperature and land use type affected the cumulative soil CO2 efflux, and DOC, DON, NH4 +-N and NO3 ?-N concentrations. The form of N added or its interaction with the other two factors did not affect any of the C and N cycling parameters.

Conclusions

Temperature and land use type were dominant factors affecting soil C loss, with the soil C in grassland soils more stable and resistant to temperature changes. The lack of short-term effects of the deposition of different N species on soil C and N mineralization suggest that maybe there was a threshold for the N effect to kick in and long-term experiments should be conducted to further elucidate the species of N deposition effects on soil C and N cycling in the studied systems.  相似文献   

6.
Zhou X Q  Wang Y F  Hao Y B 《农业工程》2012,32(4):180-183
In the Three Gorges Reservoir Region of China, periodic flooding has led to plant destruction, causing much ecological damage. Re-vegetation with submergence-tolerant species is a possible solution to this problem. At present, many submergence-tolerant species have been selected for such restoration efforts, but it is unclear why these species can survive complete submergence while other species cannot. In this study, we investigated the response of two species – submergence-tolerant Salix variegata Franch. and submergence-intolerant Cinnamomum camphora (L.) Presl. – to flooding. Plants were submerged to 2 m for 3, 9, 15, and 30 days, after which malondialdehyde (MDA) (a membrane injury product) and superoxide anion content, as well as superoxide dismutase (SOD) and peroxidase (POD) activity, was measured. We found that (1) MDA levels increased in submerged C. camphora seedlings but remained constant in S. variegata; (2) superoxide anion content and SOD activity in the two species responded similarly to submergence; and (3) POD activity in S. variegata seedlings was much higher than in C. camphora. These results demonstrate that plant tolerance to submergence is related to membrane stability, and that POD activity is an important factor in this tolerance.  相似文献   

7.
天山林区不同类型群落土壤氮素对冻融过程的动态响应   总被引:1,自引:0,他引:1  
季节性冻融过程对北方温带森林土壤氮素的转化与流失具有重要影响,但不同类型群落对冻融过程响应的差异尚不明确。通过在林地、草地、灌丛上设置系列监测样地,采用原位培养的方法,利用林冠遮挡形成的自然雪被厚度差异,监测分析了冻融期天山林区不同群落表层土壤(0—15 cm)的氮素动态及净氮矿化速率间的差异。结果表明:(1)不同类型群落土壤的铵态氮(NH+4-N)含量、微生物量氮(MBN)含量基本与土壤(5 cm)温度呈正相关,深冻期林地土壤铵态氮含量低于其他群落类型而硝态氮含量高于其他群落类型;(2)硝态氮(NO-3-N)为天山林区季节性冻融期间土壤矿质氮的主体,占比达78.4%。灌丛土壤硝态氮流失风险较大,融化末期较融化初期灌丛土壤硝态氮含量下降了64.6%;(3)冻融时期对整体氮素矿化速率影响显著,群落类型对氨化速率影响显著;(4)天山林区土壤氮素在冻结期主要以氮固持为主。通过揭示不同类型群落土壤氮素对冻融格局的响应,能够助益于对北方林区冬季土壤氮素循环的认识。  相似文献   

8.
川西北高寒草地沙化过程中土壤氮素变化特征   总被引:7,自引:0,他引:7  
蒋双龙  胡玉福  蒲琴  舒向阳  袁铖铭  余倩 《生态学报》2016,36(15):4644-4653
草地沙化是我国最严重的环境问题之一,但关于草地沙化过程中氮素变化特征的研究报道多集中于干旱半干旱地区,而半湿润地区的相关报道还比较缺乏。通过野外调查,研究了川西北半湿润地区高寒沙质草地沙化过程中土壤氮素变化特征。结果表明,草地沙化对0—100cm土层土壤氮素具有显著影响,全氮、碱解氮、铵态氮(NH_4~+-N)、硝态氮(NO_3~--N)和微生物量氮(MBN)均呈现极显著下降的变化特征,极度沙化阶段较未沙化阶段分别减少了73.95%、77.72%、76.75%、79.77%和84.12%。其中,0—20cm土层变化最显著,全氮、碱解氮、NH_4~+-N、NO_3~--N和MBN含量分别减少了86.43%、83.52%、82.11%、88.82%和91.77%。随着土层深度增加,不同程度沙化草地土壤氮素含量及其变化量逐渐减少;草地沙化过程中,不同沙化阶段土壤氮素损失数量不尽相同,其中,以轻度沙化阶段氮素损失最严重,全氮、碱解氮、NH_4~+-N、NO-3-N和MBN含量分别降低了41.18%、35.17%、46.74%、43.46%和46.88%。草地沙化过程中,土壤全氮、碱解氮、NH_4~+-N、NO_3~--N和MBN含量与土壤粉粒、粘粒含量和植被群落盖度均呈极显著正相关特征,与土壤沙粒含量呈极显著负相关特征。研究区土壤氮素损失与风蚀选择性吹蚀土壤粉粒、粘粒及地表植物覆盖状况逐渐变差密切相关,因此该区域治沙的关键是采取措施降低风蚀对地表土壤吹蚀作用,提高沙化草地地表植被覆盖。同时,还应及时对沙化前期阶段及潜在沙化的草地进行生态治理,从而避免草地沙化继续恶化。  相似文献   

9.
滇西北高原纳帕海湿地土壤氮矿化特征   总被引:4,自引:4,他引:4  
解成杰  郭雪莲  余磊朝  许静 《生态学报》2013,33(24):7782-7787
采用树脂芯原位培育法,研究了纳帕海沼泽、沼泽化草甸和草甸土壤氮的矿化特征。结果表明,铵态氮(NH4+-N)为沼泽、沼泽化草甸土壤中无机氮的主要存在形式,分别占无机氮含量的96.76%和75.24%,而硝态氮(NO3--N)为草甸土壤中无机氮的主要存在形式,占无机氮含量的58.77%。植物生长期内,纳帕海湿地土壤的净氮矿化速率表现为沼泽化草甸 > 草甸 > 沼泽,表明干湿交替的土壤环境更利于土壤氮矿化作用的进行,土壤中氮素有效性和维持植物可利用氮素的能力更强。整个生长季,沼泽和草甸土壤氮矿化为硝化作用,而沼泽化草甸土壤氮矿化为氨化作用。土壤硝态氮含量、有机质含量、碳氮比和含水量均对纳帕海沼泽、沼泽化草甸和草甸土壤的氮矿化产生显著影响。  相似文献   

10.
Understanding the factors influencing water and nutrient transport through soil profile is important for the efficient management of nutrient and irrigation to minimize nutrient leaching below the rootzone. Transport of NO3-N and NH4-N was studied in a Candler fine sand following a heavy loading of a liquid fertilizer containing ammonium nitrate. Both NO3-N and NH4-N transported quite rapidly (within 3?d) and accumulated above the clay layer at about depth of 2.7?m. The concentrations of NH4-N and NO3-N approached background levels throughout the soil profile by 184?d. More than 50% of ammonium and nitrate contained in the spilled solution leached from the entire depth of soil profile sampled during the first 95?d. The cumulative amount of rainfall during this period was 329?mm, which accounted for 65% of the total rainfall for the entire study period. The concentrations of NH4-N and NO3-N at various depths within the entire soil profile reasonably predicted by the Leaching Estimation and Chemistry Model (LEACHM) and compared favorably with the measured concentrations, however, there are few places with high concentrations. The cumulative amount of leachate at the bottom of the soil profile predicted by LEACHM represented 90% of total rainfall that occurred during the study period. This demonstrated a substantial potential for leaching of soluble nutrients through the sandy soil profile.  相似文献   

11.

Background and aims

Physical and chemical soil properties determine local plant conditions and resources, affecting plants’ ability to respond to disturbances. In alpine grasslands, wild boar disturbances occur at different intensities, what may affect differently their soil properties. Alpine soils from five contrasted plant communities were explored within and outside disturbances, accounting for an overall and community scale effect. Additionally, we analysed the effect of disturbance intensity on soil NO3 --N and NH4 +-N.

Methods

Soils were analyzed for physical (bulk density, moisture content and electrical conductivity), and chemical properties (pH, total N and C, oxidizable C, C:N ratio, available K, P, Ca2+, Na+ and Mg2+). Resin bags were used to compare the effect of the disturbance occurrence and intensity on soil NO3 --N and NH4 +-N.

Results

Bulk density, total N and NO3 --N concentration were significantly higher in disturbed areas, while soil moisture, C:N, NH4 +-N, Na+, Mg2+ and Ca2+ concentrations were significantly lower. However, low disturbance intensity reduced NO3 --N and increased NH4 +-N concentrations.

Conclusions

Wild boar occurrence and intensity strongly alter physical and chemical conditions of alpine soils, increasing soil compaction, and altering the availability of N forms. These changes may affect most plant species, thus affecting the structure and dynamics of alpine plant communities.  相似文献   

12.
李天阳  何丙辉  张海香  袭培栋 《生态学报》2023,43(10):3894-3905
明确典型农作措施对紫色土坡耕地径流氮(N)流失的影响可为优化面源污染防控措施提供科学依据。基于2019—2021年沙溪庙组发育的壤质紫色土坡耕地不同降雨等级次降雨产流事件,分析了常规施肥(CK),优化施肥(T1)和优化施肥+秸秆还田(T2)措施下径流、径流中总氮(TN)、硝态氮(NO3-N)及铵态氮(NH4-N)流失浓度和流失量的变化特征。结果表明,与CK相比,T1和T2的径流深分别减小了4.24%、12.71%,但减小程度不显著(P>0.05);CK与T1的径流系数相等,且比T2增加了12.5%(P>0.05);相比CK,T1和T2的TN浓度增加了19.35%、25.8%(P>0.05),TN流失量则均增加了11.54%(P>0.05),表明T1和T2的施用也有增加土壤N流失的潜在风险。与中雨、大雨和大暴雨事件相比,暴雨事件的径流深分别增加了6.5%—191.11%(P<0.05),而TN、NO3-N及NH4-N的流失量分别增加106.38%—177.14%(P>...  相似文献   

13.
Runoff quantity and quality from a 248 m2 extensive green roof and a control were compared in Connecticut using a paired watershed study. Weekly and individual rain storm samples of runoff and precipitation were analyzed for TKN, NO3 + NO2-N, NH3-N, TP, PO4-P, and total and dissolved Cu, Pb, Zn, Cd, Cr, and Hg. The green roof watershed retained 51.4% of precipitation during the study period based on area extrapolation. Overall, the green roof retained 34% more precipitation than predicted by the paired watershed calibration equation. TP and PO4-P mean concentrations in green roof runoff were higher than in precipitation but lower than in runoff from the control. The green roof was a sink for NH3-N, Zn, and Pb, but not for TP, PO4-P, and total Cu. It also reduced the mass export of TN, TKN, NO3 + NO2-N, Hg, and dissolved Cu primarily through a reduction in stormwater runoff. Greater than 90% of the total Cu, Hg, and Zn concentrations in the green roof runoff were in the dissolved form. The growing media and slow release fertilizer were probable sources of P and Cu in green roof runoff. Overall, the green roof was effective in reducing stormwater runoff and overall pollutant loading for most water quality contaminants.  相似文献   

14.
康希睿  张涵丹  王小明  陈光才 《生态学报》2020,40(19):6958-6968
森林群落在净化空气、截留沉降污染物、改善地表水质等方面具有重要作用。本研究以北亚热带地区3种典型森林群落(毛竹林、杉木林、青冈阔叶林)为研究对象,通过分析沉降污染物(NH4+-N、NO3--N、NO2--N、TP和SO42-)在大气降水、林内穿透雨、树干茎流、枯透水和地表径流中的浓度和通量变化特征,探讨不同森林群落对氮、磷、硫的截留净化作用和分配特征。结果表明,该区域大气降水中NH4+-N、NO3--N、NO2--N、TP和SO42-年均浓度分别为1.06、0.61、0.04、0.07、1.84 mg/L,其年均pH为5.88;各森林群落林冠层能够调升降雨的pH且全年稳定,对TP和NH4+-N均有吸附作用,截留率分别为79.09%-84.68%和30.88%-69.36%;而枯落物层则是林下氮、磷、硫的主要释放源,对NH4+-N、NO3--N、TP和SO42-均具有淋溶作用;此外,由地表径流(输出)与大气降水(输入)的对比分析可知,各林地对沉降污染物中氮、磷、硫的截留率均超过98%;3种森林群落对沉降污染物中氮、磷、硫的截留能力依次为:青冈阔叶林 > 毛竹林 > 杉木林,阔叶林对沉降污染物的净化能力要高于毛竹林及针叶的杉木林。  相似文献   

15.
Arbuscular mycorrhizal (AM) fungi have beneficial effects on host plants, but their growth is influenced by various factors. This study was carried out to analyze the variation of AM fungi in soils and roots of peach (Prunus persica L. var. Golden Honey 3, a yellow-flesh variety) trees in different soil layers (0–40 cm) and their correlation with soil properties. The peach tree could be colonized by indigenous AM fungi (2.2–8.7 spores/g soil and 1.63–3.57 cm hyphal length/g soil), achieving 79.50–93.55% of root AM fungal colonization degree. The mycorrhizal growth, root sugars, soil three glomalins, NH4+-N, NO3-N, available P and K, and soil organic matter (SOM) had spatial heterogeneity. Soil spores, but not soil hyphae contributed to soil glomalin, and soil glomalin also contributed to SOM. There was a significant correlation of soil hyphae with spore density, soil NO3-N, and SOM. Root mycorrhiza was positively correlated with spore density, NH4+-N, NO3-N, and easily extractable glomalin-related soil protein. Notably, spore density positively correlated with NO3-N, available K, SOM, and root fructose and glucose, while negatively correlated with available P and root sucrose. These findings concluded that mycorrhiza of peach showed spatial distribution, and soil properties mainly affected/altered based on the soil spore density.  相似文献   

16.
氮素类型和剂量对寒温带针叶林土壤N2O排放的影响   总被引:1,自引:0,他引:1  
大气氮沉降输入会增加森林生态系统氮素有效性,进而改变土壤N_2O产生与排放,然而有关不同氮素离子(氧化态NO_3~--N与还原态NH_4~+-N)沉降对土壤N_2O排放的影响知之甚少。以大兴安岭寒温带针叶林为研究对象,构建了3种类型(NH_4Cl、KNO_3、NH_4NO_3)和4个施氮水平(0、10、20、40 kg N hm~(-2)a~(-1))的增氮控制试验,利用流动化学分析仪和静态箱-气相色谱法4次/月测定凋落物层和矿质层土壤无机氮含量、土壤-大气界面N_2O净交换通量以及相关环境因子,分析施氮类型和剂量对土壤氮素有效性、土壤N_2O通量的影响探讨氮素富集条件下土壤N_2O通量的环境驱动机制。结果表明:施氮类型和剂量均显著影响土壤无机氮含量,土壤NH_4~+-N的积累效应显著高于NO_3~--N。施氮一致增加寒温带针叶林土壤N_2O排放,NH_4NO_3促进效应最为明显,增幅为442%-677%,高于全球平均水平(134%)。土壤N_2O通量与土壤温度、凋落物层NH_4~+-N含量正相关,且随着施氮水平增加而增加。结果表明大气氮沉降短期内不会导致寒温带针叶林土壤NO_3~--N大量流失,但会显著促进土壤N_2O的排放。此外,外源性NH_4~+和NO_3~-输入对土壤N_2O排放的促进作用具有协同效应,在未来森林生态系统氮循环和氮平衡研究中应该区分对待。  相似文献   

17.
A telescopic method for photographing within 8×8 cm minirhizotrons   总被引:1,自引:0,他引:1  
The volatile organic compounds produced during a sequence of soil incubations under controlled conditions, with either added NH4 +-N or NO3 --N, were collected and identified. The nature and relative amounts of the volatile organic compounds produced by the microorganisms in the soils were remarkably reproducible and consistent.  相似文献   

18.
绿色屋面降雨径流水质及消减污染负荷研究   总被引:5,自引:0,他引:5  
2011年5月至11月对12场降雨时段的屋面径流和干湿沉降进行采样监测,比较了屋面径流(绿色屋面和沥青屋面)、干湿总沉降和降雨中污染物的浓度和污染负荷,并运用多元统计方法分析了降雨特征对绿色屋面径流水质的影响。结果表明,从径流水质层面上分析,绿色屋面是总悬浮物(TSS)的汇,对p H值有较好的中和作用,对于总磷(TP)、溶解态铜(DCu)和溶解态锌(DZn)是非源非汇,是电导率(EC)、总氮(TN)、氨态氮(NH+4-N)、硝态氮(NO-3-N)、化学需氧量(COD)、5日生化需氧量(BOD5)和溶解态铅(DPb)的源;从污染负荷的角度分析,绿色屋面是NH+4-N、TSS和BOD5的汇,对于TN、DPb、DCu、DZn、TP和COD是非源非汇,是NO-3-N的源;与控制屋面相比,绿色屋面可以消减TSS、TP、BOD5、COD、NH+4-N、DZn和DPb的污染负荷,分别消减了90.53%、49.38%、41.31%、36.48%、35.45%、28.27%和14.20%;但是增加了NO-3-N和TN的污染负荷,分别增加了821.02%和275.48%;绿色屋面径流污染物的浓度与降雨量、降雨历时和降雨强度呈负相关关系,而与降雨间隔呈正相关关系。研究结果为绿色屋面的科学设计及正确评价绿色屋面对径流水质的影响提供依据。  相似文献   

19.
The dynamics of inorganic N are important in soil, and this applies particularly to the saline–alkaline soils of the former lake Texcoco in Mexico with high pH and salinity where a forestation program was started in the 1970s. In soils of lake Texcoco, in Mexico, more than 50% of applied N could not be accounted for one day after application of 200 mg kg–1 soil along with glucose amendment. It was not clear whether this was due to abiotic or biotic processes, the form of inorganic N applied or the result of applying an easily decomposable substrate. We investigated this by adding glucose and 200 mg kg–1 soil as (NH4)2SO4-N or KNO3-N to sterilized and unsterilized soil. The changes in inorganic and ninhydrin N, microbial biomass C and production of CO2 were then monitored. Between the time of applying N and extraction with 0.5 M K2SO4, i.e., after ca 2 h, approximately 110 mg NH4 +-N kg–1 dry soil could not be accounted for in the unsterilized and sterilized soil and that remained so for the entire incubation in the sterilized soil. After 1 day this increased to 140 mg NH4 +-N kg–1 dry soil in the unsterilized control and 170 mg NH4 +-N kg–1 dry soil in C amended soil. Volatilization of NH3 accounted for 56 mg NH4 +-N kg–1 so the rest appeared to be adsorbed on the soil matrix. The NH3 volatilization and NH4 + fixed in the soil matrix remained constant over time and no oxidation to NO2 or NO3 had occurred, so unaccounted N in unsterilized soil was probably incorporated into the microbial biomass in excess of what was required for metabolic activity. The unaccounted N was ca 70 mg NO3 –N in nitrate amended soil after 3 days and 138 NO3 –N when glucose was additionally added. Losses through abiotic processes were absent as inferred from changes in sterilized soil and the aerobic incubation inhibited possible losses through denitrification. It was inferred that NO3 that could not be accounted for was taken up by micro-organisms in excess of what was required for metabolic activity.  相似文献   

20.
左倩倩  王邵军  王平  曹乾斌  赵爽  杨波 《生态学报》2021,41(18):7339-7347
蚂蚁作为生态系统工程师能够调节土壤微生物及理化环境,进而对热带森林土壤有机氮矿化速率及其时间动态产生显著影响。以西双版纳白背桐热带森林群落为研究对象,采用室内需氧培养法测定土壤有机氮矿化速率,比较蚁巢和非蚁巢土壤有机氮矿化速率的时间动态,揭示蚂蚁筑巢活动引起土壤无机氮库、微生物生物量碳及化学性质改变对有机氮矿化速率时间动态的影响。结果表明:(1)蚂蚁筑巢显著影响土壤有机氮矿化速率(P<0.01),相较于非蚁巢,蚁巢土壤有机氮矿化速率提高了261%;(2)土壤有机氮矿化速率随月份推移呈明显的单峰型变化趋势,即6月最大(蚁巢1.22 mg kg-1 d-1、非蚁巢0.41 mg kg-1 d-1),12月最小(蚁巢0.82 mg kg-1 d-1、非蚁巢0.18 mg kg-1 d-1);(3)两因素方差分析表明,不同月份及不同处理对土壤有机氮矿化速率、NH4-N及NO3-N产生显著影响(P<0.05),但对NO3-N的交互作用不显著;(4)蚂蚁筑巢显著提高了无机氮库(NH4-N与NO3-N)、微生物生物量碳、有机质、水解氮、全氮及易氧化有机碳等土壤养分含量,而降低了土壤pH值;(5)回归分析表明,铵态氮和硝态氮对土壤有机氮矿化速率产生显著影响,分别解释87.89%、61.84%的有机氮矿化速率变化;(6)主成份分析表明NH4-N、微生物生物量碳及有机质是影响有机氮矿化速率时间动态的主要因素,而全氮、NO3-N、易氧化有机碳、水解氮及pH对土壤有机氮矿化速率的影响次之,且pH与土壤有机氮矿化速率呈显著负相关。总之,蚂蚁筑巢活动主要通过影响土壤NH4-N、微生物生物量碳及有机质的状况,进而调控西双版纳热带森林土壤有机氮矿化速率的时间动态。研究结果将有助于进一步提高对土壤氮矿化生物调控机制的认识。  相似文献   

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