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K. INGENLOFF R. GARLAPATI D. POCHÉ M. I. SINGH J. L. REMMERS R. M. POCHÉ 《Medical and veterinary entomology》2013,27(1):10-18
Three rodent feed‐through studies were conducted to evaluate the efficacy of insecticides to control Phlebotomus argentipes (Diptera: Psychodidae). The initial test evaluated diflubenzuron, eprinomectin, fipronil and ivermectin as feed‐through treatments in Rattus rattus (Rodentia: Muridae). In the preliminary trial, all four insecticides yielded 100% mortality of P. argentipes larvae within 20 days of exposure to treated rodent faeces. Based upon the initial results, fipronil was evaluated further as a feed‐through utilizing Bandicota bengalensis (Rodentia: Muridae). The B. bengalensis trial evaluated fipronil against both adult and larval sandflies at 250 p.p.m., 100 p.p.m. and 50 p.p.m. The results showed the fipronil treatment to have 100% efficacy against larvae up to 20 days post‐treatment and over 74% efficacy against adult sandflies presented with B. bengalensis faeces up to 10 days post‐treatment at all three dosage levels. The results of the three studies suggest that all four insecticides may be useful tools with which to control Leishmania vector populations. 相似文献
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H. Bakhshi M.A. Oshaghi M.R. Abai Y. Rassi A.A. Akhavan Z. Sheikh F. Mohtarami Z. Saidi H. Mirzajani M. Anjomruz 《Experimental parasitology》2013
A molecular study was carried out to incriminate sand fly vectors of cutaneous leishmaniasis (CL) in rural areas of Sarakhs district, Khorassane-Razavi Province, northeastern Iran, in 2011. Sand flies of Sergentomyia with three species and Phlebotomus with six species respectively comprised 73.3% and 26.7% of the specimens. Phlebotomus papatasi was the most common Phlebotomine species in outdoor and indoor resting places. Leishmania infection was found at least in 17 (22%) specimens including Ph. papatasi (n = 9 pool samples), Phlebotomus caucasicus (n = 6), Phlebotomus alexandri (n = 1), and Sergentomyia sintoni (n = 1). The parasites were found comprised Leishmania major (n = 5), Leishmania turanica (n = 10), and Leishmania gerbilli (n = 4). Infection of Ph. papatasi with both L. major and L. turanica supporting the new suggestion indicating that it is not restricted only with L. major. Circulation of L. major by Ph. alexandri, and both L. gerbilli and L. turanica by Ph. caucasicus, in addition to previous data indicating the ability of Ph. alexandri to circulate Leishmania infantum and Leishmania donovani, and Ph. caucasicus to circulate L. major, suggests that these two species can be permissive vectors. The results suggest that Ph. papatasi and Ph. alexandri are the primary and secondary vectors of CL where circulating L. major between human and reservoirs, whereas Ph. caucasicus is circulating L. turanica and L. gerbilli between the rodents in the region. 相似文献
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Ido Tsurim Gideon Wasserberg Alon Warburg Ibrahim Abbasi Gil Ben Natan Zvika Abramsky 《Medical and veterinary entomology》2023,37(1):4-13
Systemic control uses the vertebrate hosts of zoonotic pathogens as “Trojan horses,” killing blood-feeding female vectors and short-circuiting host-to-vector pathogen transmission. Previous studies focused only on the effect of systemic control on vector abundance at small spatial scales. None were conducted at a spatial scale relevant for vector control and none on the effect of systemic control on pathogen transmission rates. We tested the application of systemic control, using Fipronil-impregnated rodent baits, in reducing Leishmania major (Kinetoplastida: Trypanosomatidae; Yakimoff & Schokhor, 1914) infection levels within the vector, Phlebotomus papatasi (Diptera: Psychodidae; Scopoli, 1786) population, at the town-scale. We provided Fipronil-impregnated food-baits to all Psammomys obesus (Mammalia:Muridae; Cretzschmar, 1828), the main L. major reservoir, burrows along the southern perimeter of the town of Yeruham, Israel, and compared sand fly abundance and infection levels with a non-treated control area. We found a significant and substantial treatment effect on L. major infection levels in the female sand fly population. Sand fly abundance was not affected. Our results demonstrate, for the first time, the potential of systemic control in reducing pathogen transmission rates at a large, epidemiologically relevant, spatial scale. 相似文献
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Samir S. Sawalha Asad Ramlawi Ramzi M. Sansur Ibrahim Mohammad Salem Zuhair S. Amr 《Journal of vector ecology》2017,42(1):120-129
The diversity, ecology, and seasonality for sand flies from two localities in Jenin District, the Palestinian Territories, were studied. A total of 12,579 sand flies (5,420 Phlebotomus and 7,159 Sergentomyia) were collected during the study period. The genera Phlebotomus and Sergentomyia are represented by 13 and nine species and subspecies, respectively. Species account was given for all collected species. CDC light traps yielded 7,649 (60.8%) of the total captured sand flies, while sticky traps and aspirators contributed to 36.4 and 2.8% of the total collected specimens, respectively. Phlebotomus sergenti and P. syriacus showed two peaks, one in July and one in October. Phlebotomus tobbi showed one peak towards the end of the summer in September and August, while P. papatasi showed a bimodal peaks pattern, one in June and one in October. Phlebotomus canaaniticus showed a peak in August. P. perfiliewi transcaucasicus and P. neglectus showed a peak in October. Sergentomyia dentata showed one peak in August and increasing numbers from June to August, declining afterwards. Other species, such as S. theodori, had one peak in June, S. taizi had steady numbers across the summer, and S. christophersi had a peak in August. 相似文献
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The species composition and seasonal abundance of sand flies (Diptera: Phlebotominae) were studied in the years 2000-2002 in the Sanliurfa region, which is the largest focus of cutaneous leishmaniasis in south-eastern Turkey. Sixteen species were identified among 29 771 specimens collected at 17 different sites by light traps, sticky papers and aspirators. The most common species were Phlebotomus papatasi (Scopoli) (45.4%), P. perfiliewi Parrot (21.9%), and P. sergenti Parrot (19.4%). The other species found were P. major Adler & Theodor (3%), P. neglectus Leger & Pesson (2.2%), P. brevis Theodor & Mesghali (2%), P. alexandri Sinton (1.9%), P. galilaeus Theodor (1.6%), P. halepensis Theodor (0.84%), Sergentomyia adleri Theodor (0.78%), S. dentata Sinton (0.49%), S. minuta Rondani (0.42%), S. theodori Parrot (0.16%), P. kazeruni Theodor & Mesghali (0.001%) and P. mascitti Grassi (0.001%) and one unidentified Phlebotomus species. Among these species P. galilaeus, S. minuta and S. dentata are the first records for this area. All species showed seasonal fluctuations, with the period of highest abundance between May and October. 相似文献
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Kárin Rosi Reinhold‐Castro Jaqueline de Carvalho Gasparotto Herintha Coeto Neitzke‐Abreu Ueslei Teodoro 《Journal of vector ecology》2015,40(2):269-276
We report the results of an investigation of natural larval sand fly habitats in the Recanto Marista, Doutor Camargo municipality, Paraná state, Brazil, from May, 2010 to August, 2012. We used Alencar emergence traps (AT), experimental traps (ET), and soil samples incubated in a biochemical oxygen demand incubator. Eight sand flies were collected with ATs. One specimen was collected with an ET and 21 were collected in soil samples. The collected species were Brumptomyia brumpti, Micropygomyia ferreirana, Migonemyia bursiformis, Migonemyia migonei, Nyssomyia neivai, Nyssomyia whitmani, and Pintomyia pessoai. The laval habitats of sand flies were located in the Recanto Marista, especially between tree roots, but the number of adults that emerged in the traps and soil samples was small despite the high density of sand flies that has been recorded in the Recanto Marista. 相似文献
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Arthur Kocher Jean‐Charles Gantier Pascal Gaborit Lucie Zinger Helene Holota Sophie Valiere Isabelle Dusfour Romain Girod Anne‐Laure Bañuls Jerome Murienne 《Molecular ecology resources》2017,17(2):172-182
Phlebotomine sand flies are haematophagous dipterans of primary medical importance. They represent the only proven vectors of leishmaniasis worldwide and are involved in the transmission of various other pathogens. Studying the ecology of sand flies is crucial to understand the epidemiology of leishmaniasis and further control this disease. A major limitation in this regard is that traditional morphological‐based methods for sand fly species identifications are time‐consuming and require taxonomic expertise. DNA metabarcoding holds great promise in overcoming this issue by allowing the identification of multiple species from a single bulk sample. Here, we assessed the reliability of a short insect metabarcode located in the mitochondrial 16S rRNA for the identification of Neotropical sand flies, and constructed a reference database for 40 species found in French Guiana. Then, we conducted a metabarcoding experiment on sand flies mixtures of known content and showed that the method allows an accurate identification of specimens in pools. Finally, we applied metabarcoding to field samples caught in a 1‐ha forest plot in French Guiana. Besides providing reliable molecular data for species‐level assignations of phlebotomine sand flies, our study proves the efficiency of metabarcoding based on the mitochondrial 16S rRNA for studying sand fly diversity from bulk samples. The application of this high‐throughput identification procedure to field samples can provide great opportunities for vector monitoring and eco‐epidemiological studies. 相似文献
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B. M. El Sawaf;N. Helmy;H. A. Kamal;A. Osman;M. Shehata 《Parasite (Paris, France)》2016,66(3):134-136
Soil analysis was carried out on samples obtained from breeding sites of the sanflies Phlebotomus langeroni Nitzulescu and Phlebotomus papatasi (Scopoli) and compared to non-breeding sites.Soil characterized by relatively high moisture content, high organic matter and derivatives, such as phosphorous potassium and nitrogen, high percent of silt and slightly alkaline pH, constitutes peculiar conditions which favour immature development of both species.https://doi.org/10.1051/parasite/1991663134 相似文献
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[目的]为提高水晶梨病虫害防治工作效率,进一步提升病虫害的预测效果和精度。[方法]深入研究了灰色模型(GM),利用GM对水晶梨环境因子数据进行建模得到病虫害预测公式,通过差分方程推导出时间响应式和参数估计,建立了优化初始值的灰色模型(OIVGM),将OIVGM与BP神经网络预测模型(BP)进行组合,建立了优化初始值的灰色BP神经网络预测组合模型(OIVGM-BP)。[结果]通过单位根检验法测量模型的稳定性,OIVGM-BP一阶差分处理后,T统计量(-5.487654)小于5%临界值(-2.878073),数据序列表明平稳,OIVGM-BP可以稳定进行预测。通过白噪声检验方法测量OIVGM-BP的适应性,OIVGM-BP的残差P值从第二阶开始,均大于0.05,说明OIVGM-BP的适应性较好,各阶均通过了白噪声检验。LRM、GM、TSM、BP、OIVGM-BP对梨锈病、白粉病、腐烂病、梨黄粉蚜、梨二叉蚜、梨木虱6种病虫害的预测准确率的平均值分别为70.81%、70.09%、69.74%、65.64%、83.01%,OIVGM-BP的预测准确率优于其他4种预测模型。[结论]OIVGM-BP能够对水晶梨病虫害进行有效预测,能够更好地指导农业生产。 相似文献
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A 3‐chamber in‐line olfactometer designed for use with sand flies is described and tested as a high‐throughput method to screen honeys for attractiveness to Phlebotomus papatasi (four geographic isolates), P. duboscqi (two geographic isolates), and Lutzomyia longipalpis maintained in colonies at the Walter Reed Army Institute of Research. A diversity of unifloral honey odors were evaluated as a proxy for the natural floral odors that sand flies may use in orientation to floral sugar sources in the field. In the 3‐chamber in‐line olfactometer, the choice modules come directly off both sides of the release area instead of angling away as in the Y‐tube olfactometer. Of the 25 honeys tested, five had a significant attraction for one or more of the sand fly isolates tested. This olfactometer and high‐throughput method has utility for evaluating a diversity of natural materials with unknown complex odor blends that can then be down‐selected for further evaluation in wind tunnels and/or field scenarios. 相似文献
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Nilka Luisa Díaz Olga Zerpa Félix Jacobo Tapia 《Memórias do Instituto Oswaldo Cruz》2013,108(4):446-452
American cutaneous leishmaniasis (ACL) presents distinct activeclinical forms with different grades of severity, known as localised (LCL),intermediate (ICL) and diffuse (DCL) cutaneous leishmaniasis. LCL and DCL areassociated with a polarised T-helper (Th)1 and Th2 immune response,respectively, whereas ICL, or chronic cutaneous leishmaniasis, is associatedwith an exacerbated immune response and a mixed cytokine expression profile.Chemokines and chemokine receptors are involved in cellular migration and arecritical in the inflammatory response. Therefore, we evaluated the expression ofthe chemokines CXCL10, CCL4, CCL8, CCL11 and CXCL8 and the chemokine receptorsCCR3, CXCR3, CCR5 and CCR7 in the lesions of patients with different clinicalforms of ACL using immunohistochemistry. LCL patients exhibited a high densityof CXCL10+, CCL4+ and CCL8+ cells, indicating an important role for thesechemokines in the local Th1 immune response and the migration of CXCR3+ cells.LCL patients showed a higher density of CCR7+ cells than ICL or DCL patients,suggesting major dendritic cell (DC) migration to lymph nodes. Furthermore, DCLwas associated with low expression levels of Th1-associated chemokines andCCL11+ epidermal DCs, which contribute to the recruitment of CCR3+ cells. Ourfindings also suggest an important role for epidermal cells in the induction ofskin immune responses through the production of chemokines, such as CXCL10, bykeratinocytes. 相似文献
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B Alexander L. A. Agudelo F. Navarro F. Ruiz J. Molina G. Aguilera M. L. Quiñones 《Medical and veterinary entomology》2001,15(4):364-373
The phlebotomine sandfly fauna of traditional (shaded) and intensified (unshaded) coffee plantations in Colombia was sampled by a variety of methods and the species composition and density under the two systems compared. Twenty species of Lutzomyia sandflies (Diptera: Psychodidae: Phlebotominae) were collected, of which eight were found only in the 'Coffee Axis' ('Eje Cafetero') of the departments of Caldas, Risaralda and Quindio, six were exclusive to the department of Norte de Santander and six occurred in both regions. Four species were collected only in traditional plantations and two exclusively in intensified ones. At least 13 species occurred in both plantation types. Fifteen species are opportunistic man-biters and eight are suspected vectors of leishmaniasis caused by Le. braziliensis, Le. panamensis or Le. mexicana. Seven species were collected inside houses and may be involved in intradomiciliary transmission of Leishmania. The dominant species in Norte de Santander was Lu. spinicrassa, which made up 93.8% of all the sandflies collected in this department. This species was absent from the Eje Cafetero and a number of others among the 15 recorded there might be responsible for Leishmania transmission in this region, including Lu. trapidoi, Lu. yuilli, Lu. gomezi, L. hartmanni and Lu. ovallesi. Sandfly population densities were significantly higher in traditional plantations than in intensified ones. Residents of traditional plantations were able to describe sandflies in significantly more detail than those of intensified plantations, based on seven basic characteristics related to the appearance and biting behaviour of the insects. 相似文献
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Vectors of cutaneous leishmaniasis in north-central Venezuela 总被引:1,自引:0,他引:1
M. DORA FELICIANGELI NORIS RODRIGUEZ ARTURO BRAVO FREDDY ARIAS BERNARDO GUZMAN 《Medical and veterinary entomology》1994,8(4):317-324
Abstract. An entomological survey was undertaken from January 1991 to February 1992 in El Ingenio, Miranda State, Venezuela, an endemic area of cutaneous leishmaniasis: prevalence of 10.7 cases per 100,000 inhabitants.
A total of 4863 female sandflies (Phlebotominae) of fourteen species were collected in Shannon traps, then dissected and examined for leishmanial infections.
Lutzomyia ovallesi (85.4%) and Lu. gomezl (11.2%) were the predominant anthropophilic species of sandfly. Fifty-one (1.19%) Lu. ovallesi and two(0.47%) Lu. gomezi had natural infection with Leishmania promastigotes. Identification of the parasites was done by using the polymerase chain reaction (PCR) and DNA hybridization. Two isolates from Lu. gomezi and forty-nine from Lu. ovallesi were typed as Leishmania braziliensis and three of the latter reacted with Le. mexicana also. This is the first report of Lu. gomezi with parasites typed as Le. braziliensis.
We concluded that Lu. ovallesi is the primary vector of cutaneous leishmaniasis in the north-central area of Venezuela and Lu. gomezi should be regarded as an additional vector. 相似文献
A total of 4863 female sandflies (Phlebotominae) of fourteen species were collected in Shannon traps, then dissected and examined for leishmanial infections.
Lutzomyia ovallesi (85.4%) and Lu. gomezl (11.2%) were the predominant anthropophilic species of sandfly. Fifty-one (1.19%) Lu. ovallesi and two(0.47%) Lu. gomezi had natural infection with Leishmania promastigotes. Identification of the parasites was done by using the polymerase chain reaction (PCR) and DNA hybridization. Two isolates from Lu. gomezi and forty-nine from Lu. ovallesi were typed as Leishmania braziliensis and three of the latter reacted with Le. mexicana also. This is the first report of Lu. gomezi with parasites typed as Le. braziliensis.
We concluded that Lu. ovallesi is the primary vector of cutaneous leishmaniasis in the north-central area of Venezuela and Lu. gomezi should be regarded as an additional vector. 相似文献
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芽胞杆菌防治烟草病虫害的研究进展 总被引:1,自引:0,他引:1
《氨基酸和生物资源》2016,(3):7-11
烟草病虫害严重影响烟草产业的可持续发展,更为安全的生物防治方法已成为烟草病虫害防治研究的热点领域。芽胞杆菌属(Bacillus)是一类比较理想的生防微生物,在植病生物防治领域显示出了广阔的应用前景。本文论述了芽胞杆菌属细菌的生物学特性及其在烟草黑胫病、赤星病、青枯病、炭疽病、根黑腐病、花叶病、白粉病、烟草斜纹夜蛾和甲虫等烟草病虫害防治中的应用。 相似文献