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Renze J Plath M Ducho C Balzarini J De Clercq E Meier C 《Nucleosides, nucleotides & nucleic acids》2001,20(4-7):931-934
Synthetic routes to benzyl-functionalized cycloSal-d4T monophosphates (7CH2X-cycloSal-d4TMP) have been developed. Their hydrolytic behavior in basic aqueous solution (pH = 7.3) was studied and their hydrolysis half-lives were determined. It turned out that two different degradation pathways are leading to different products: beside the formation of the expected d4TMP and a styrene type derivative, a phenyl-d4T-phosphodiester was obtained as well. The product distribution was specified. 相似文献
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J. M. Young 《The Biochemical journal》1970,118(5):719-731
1. Mitochondria from ox heart and rat liver catalysed a slow cyanide-sensitive oxidation of 2,3-dimethylnaphthaquinol monophosphate, duroquinol monophosphate, menadiol 1-phosphate and menadiol 4-phosphate. 2. The release of P(i) was concomitant with oxygen uptake. 3. The oxidation was somewhat stimulated by Ca(2+) and P(i), and weakly inhibited by 2,4-dinitrophenol. 4. The quinol monophosphates effected a rapid reduction of free cytochrome c, and consequently addition of cytochrome c greatly increased the rate of the mitochondrial oxidation of 2,3-dimethylnaphthaquinol monophosphate. 5. This quinol phosphate interacts with the electron-transport chain at the level of cytochrome c. 6. Polylysine promoted an interaction between 2,3-dimethylnaphthaquinol monophosphate and cytochrome oxidase. Thus, although polylysine blocks mitochondrial oxidations via reduced cytochrome c, the oxidation of the quinol phosphate was strongly stimulated. 7. This stimulation was most effective in the most intact mitochondrial preparations and was inhibited by ADP and by P(i). 8. The implications of these results for factors limiting the rate of quinol phosphate oxidation, the mode of action of stimulators and the mechanism of P(i) formation are discussed. 相似文献
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Tautomerism in cytidine 总被引:1,自引:0,他引:1
The large change in the electronic absorption spectrum of cytidine on going from an aqueous to an aprotic medium is attributed to the existence of a hydrogen bonded solvent-solute complex in solvents capable of donating a proton. The spectrum of cytidine in a variety of solvents and the spectra of a number of nontautomerising model compounds are presented. The tautomerisin of cytidine and its biological implications are discussed. 相似文献
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Inositol monophosphates from the mycelia ofPholiota nameko were analyzed by gas chromatography. Mycelia were found to contain inositol-1-phosphate and inositol-2-phosphate isomers
in a proportion of 7∶1, the amounts being 50 and 7.2 pmol/mg dry weight, respectively. We assume that inositol-2-phosphate
is a hydrolysis product of the phytase reaction with phosphatase. It was also found that the amount of inositol monophosphates
in the mycelia was affected by the concentration of inorganic phosphate in the medium. 相似文献
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A contaminant compound has been isolated from commercial samples of CTP by ion-exchange chromatography on a Dowex-1 column. It has been characterized as cytidine 5'-tetraphosphate from its ultraviolet spectrum, labile and total phosphate content, and periodate consumption. It is present in proportions from 0.3 to 3.9%, apparently regardless of the method of preparation, age of sample, or commericial source of CTP. 相似文献
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Swiatek-Kozlowska J Brasuń J Dobosz A Sochacka E Glowacka A 《Journal of inorganic biochemistry》2003,93(3-4):119-124
Potentiometric and spectroscopic data obtained for the complexes of two thio-substituted uridine-monophosphates with Cu(2+), Ni(2+) and Cd(2+) ions have shown that both thionucleotide are more effective ligands than their nucleoside analogues. The basic binding site for all metal ions is the sulfur atom. The chelation by adjacent N(3) donor is also likely, although unfavorable four-membered chelate ring is formed. 相似文献
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A comparative analysis of the migration of phosphoamino acids and nucleoside monophosphates in three different two-dimensional systems is presented. The three phosphoamino acids studied are phosphoserine, phosphothreonine, and phosphotyrosine, which are the residues most commonly occurring in proteins phosphorylated by protein kinases. Their migration properties have been compared to those of UMP, AMP, CMP, GMP, and TMP, which are the basic components of nucleic acids. Special attention has been paid to the behavior of UMP, which has previously been reported to often co-migrate with phosphoamino acids. Also, the migration of inorganic orthophosphate and ribose monophosphate, which are frequently present in samples derived from macromolecule hydrolysis, has been analyzed. The following separating systems have been used: double chromatography, electrophoresis followed by chromatography, and double electrophoresis. The latter is shown to have the best resolving power and to be the most convenient system. 相似文献
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Purification and characterization of cytidine 5''-triphosphate:cytidine 5''-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase 总被引:5,自引:4,他引:1
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Cytidine 5'-triphosphate:cytidine 5'-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) was purified 2,300-fold from frozen Escherichia coli B cells. The enzyme catalyzed the formation of CMP-KDO, a very labile product, from CTP and KDO. No other sugar tested could replace KDO as an alternate substrate. Uridine 5'-triphosphate at pH 9.5 and deoxycytidine 5'-triphosphate at pH 8.0 and 9.5 could be used as alternate substrates in place of CTP. CMP-KDO synthetase required Mg2+ at a concentration of 10.0 mM for optimal activity. The pH optimum was determined to be between 9.6 and 9.3 in tris(hydroxymethyl)aminomethane-acetate or sodium-glycine buffer. This enzyme had an isoelectric point between pH 4.15 and 4.4 and appeared to be a single polypeptide chain with a molecular weight of 36,000 to 40,000. The apparent Km values for CTP and KDO in the presence of 10.0 mM Mg2+ were determined to be 2.0 X 10(-4) and 2.9 X 10(-4) M, respectively, at pH 9.5. Uridine 5'-triphosphate and deoxycytidine 5'-triphosphate had apparent Km values of 8.8 X 10(-4) and 3.4 X 10(-4) M. respectively, at pH 9.5. 相似文献
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Fluorescent adenosine and cytidine derivatives 总被引:33,自引:0,他引:33
J R Barrio J A Secrist N J Leonard 《Biochemical and biophysical research communications》1972,46(2):597-604
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The thin-layer chromatography of dinucleoside monophosphates on plates coated with DEAE-cellulose powder and on plates coated with cellulose impregnated with polyethyleneimine, together with their ionophoretic mobilities on DEAE-cellulose paper, is described. It is shown that the 2′→5′ dinucleoside monophosphates can be separated from the corresponding 3′→5′ isomers by ion-exchange thin-layer chromatography and paper ionophoresis. The method is very sensitive and can replace the commonly used enzymic hydrolysis for analyzing the nature of the phosphodiester linkage of a given dinucleoside monophosphate. 相似文献
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Wang Q Scheigetz J Gilbert M Snider J Ramachandran C 《Biochimica et biophysica acta》1999,1431(1):14-23
A series of novel fluorescein monophosphates aimed as substrates for protein tyrosine phosphatases (PTPs) were synthesized and evaluated against fluorescein diphosphate (FDP), the currently used fluorescent substrate for PTPs. In contrast to FDP, which is dephosphorylated to monophosphate and then to fluorescein in a sequential reaction, these monophosphates are dephosphorylated in a single step. This eliminates the complication in assaying PTPs due to the cleavage of the second phosphate group. The kinetic studies of these substrates with PTPs were performed and Michaelis-Menten parameters were obtained. These designed substrates have Km 0.03-0. 35 mM, kcat/Km of 3-100 mM-1 s-1 with CD45 and PTP1B. The results showed that the substrates with negative charge groups on the fluorescein have higher affinities for PTP1B, which are consistent with other observations. In this series, fluorescein monosulfate monophosphate (FMSP) was the best substrate observed. Since FMSP showed large increases in both absorption and fluorescence upon dephosphorylation by PTPs at pH>6.0, it is one of the most sensitive, stable and high affinity substrates reported for PTPs. 相似文献