共查询到20条相似文献,搜索用时 15 毫秒
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Mutant tyrosine transfer ribonucleic acids 总被引:11,自引:0,他引:11
J N Abelson M L Gefter L Barnett A Landy R L Russell J D Smith 《Journal of molecular biology》1970,47(1):15-28
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Crystallization of transfer ribonucleic acids 总被引:6,自引:0,他引:6
A compilation of crystallization experiments of tRNAs published in literature as well as original results are given and discussed in this paper. Up to now 17 different tRNA species originating from Escherichia coli and from the yeast Saccharomyces cerevisiae have been crystallized. All structural tRNA families are represented, namely the tRNAs with large or small extra-loops and among them the initiator tRNAs. The tRNAs with small variable loops (4 to 5 nucleotides), e.g. tRNAAsp and tRNAPhe, yield the best diffracting crystals. Crystalline polymorphism is a common feature; about 100 different crystal forms have been observed, but only 6 among them enabled structure determination studies by X-ray diffraction. Crystallization strongly depends upon experimental parameters such as the presence of polyamines and magnesium as well as upon the purity and the molecular integrity of the tRNAs. Crystals are usually obtained by vapour diffusion methods using salts (e.g. ammonium sulfate), organic solvents (e.g. isopropanol, dioxane or 2-methyl-2,4-pentane diol) or polyethylene glycol as precipitants. A methodological strategy for crystallyzing new tRNA species is described. 相似文献
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More mutant tyrosine transfer ribonucleic acids 总被引:27,自引:0,他引:27
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Irreversible heat inactivation of transfer ribonucleic acids 总被引:8,自引:0,他引:8
T Lindahl 《The Journal of biological chemistry》1967,242(8):1970-1973
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B J Ortwerth 《Biochemistry》1971,10(23):4190-4197
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The structures of two serine transfer ribonucleic acids 总被引:12,自引:0,他引:12
H G Zachau D Dütting H Feldmann 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1966,347(4):212-235