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1.
Electrochemical determination of N-oxidized procainamide metabolites and functional assessment of effects on murine cells in vitro 总被引:1,自引:0,他引:1
J F Wheeler C E Lunte W R Heineman L Adams E V Hess 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1988,188(3):381-386
Because of the implication of N-oxidized metabolites of procainamide in the induction of drug-related lupus, we have studied the electrochemical behavior of these metabolites and developed an electrochemical synthesis of nitrosoprocainamide. This synthesis was developed using procainamide hydroxylamine as the starting material which was oxidized to the nitroso species at an applied potential of 700 mV vs Ag/AgCl using a carbon packed bed bulk electrolysis flow cell. Conversion efficiencies of greater than 95% were achieved with this method. Subsequent studies with a chemically diverse series of biocompounds were used to investigate possible reactions between the procainamide hydroxylamine and nitroso species and these selected molecules. Only antioxidants such as cysteine, glutathione and ascorbic acid were found to react with the nitroso compound as determined by electrochemical methods, and this reaction was characterized as primarily a simple redox reaction at physiological pH. Animal studies conducted with murine spleen cells incubated with mitogens and various procainamide compounds demonstrated that the N-oxidized metabolites are the active immunopharmacologic agents. 相似文献
2.
R. M. Reeve 《American journal of botany》1959,46(3):210-217
Reeve , R. M. (U.S.D.A., Albany, California.) Histological and histochemical changes in developing and ripening peaches. I. The catechol tannins. Amer. Jour. Bot. 46(3) : 210-217. Illus. 1959.—Selective histochemical tests for catechol derivatives revealed marked changes in tannin contents during the development and ripening of peaches. The principal test used was a nitroso reaction found to be colorimetrically selective for catechol and derivatives such as chlorogenic acid. Intensities of color produced by this test were photometrically measured. Increases in catechol derivatives, so indicated in situ, were associated with early maturation and lignification of the endocarp sclereids and particularly with cessation of cell divisions and early cell enlargement of the mesocarp parenchyma. Characteristic progressive localizations of tannins in patches of enlarging mesocarp parenchyma cells, as revealed by staining with iron salts as well as by the nitroso reaction, were observed in green fruits approaching maturity. The insoluble tannin color complexes produced by these tests remained coarsely granular and intense in green fruit. Upon ripening of the fruit color intensity produced by the nitroso reaction decreased and the insoluble tannin complexes were more finely divided. The association of phenols with cellular differentiation, lignification and suberization are briefly discussed. 相似文献
3.
K A Holm 《Analytical biochemistry》1978,84(2):522-532
An automated method for the determination of cellulase activity has been developed. The enzyme incubation with the cellulose substrate is stopped at alkaline pH, after which the reaction mixture is dialyzed. The concentration of reducing sugar in the dialyzer recipient is determined by means of the colorimetric ferricyanide-molybdoarsenic acid reaction. In this reaction the ferrocyanide formed by the ferricyanide reaction is coupled with molybdoarsenic acid during formation of a molybdenum-blue color. An optimization of the analytical conditions and the reagent concentrations has been carried out. The result of the automation is an analysis with high precision (mean = 36.4; SD = 0.7%) and accuracy as well as high rate of analysis. The sensitivity is 5 mg of glucose/liter. 相似文献
4.
Siddabasave Gowda B. Gowda Seigo Usuki Mostafa A. S. Hammam Yuta Murai Yasuyuki Igarashi Kenji Monde 《Journal of lipid research》2016,57(2):325-331
Sphingoid base derivatives have attracted increasing attention as promising chemotherapeutic candidates against lifestyle diseases such as diabetes and cancer. Natural sphingoid bases can be a potential resource instead of those derived by time-consuming total organic synthesis. In particular, glucosylceramides (GlcCers) in food plants are enriched sources of sphingoid bases, differing from those of animals. Several chemical methodologies to transform GlcCers to sphingoid bases have already investigated; however, these conventional methods using acid or alkaline hydrolysis are not efficient due to poor reaction yield, producing complex by-products and resulting in separation problems. In this study, an extremely efficient and practical chemoenzymatic transformation method has been developed using microwave-enhanced butanolysis of GlcCers and a large amount of readily available almond β-glucosidase for its deglycosylation reaction of lysoGlcCers. The method is superior to conventional acid/base hydrolysis methods in its rapidity and its reaction cleanness (no isomerization, no rearrangement) with excellent overall yield. 相似文献
5.
A colorimetric method is described which can be used for the routine determination of primary and secondary N-hydroxylamino compounds in drug metabolism studies using microsomal preparations. The assay is carried out on the supernatant obtained after protein precipitation of the incubation mixture. The method is based on the reduction of ferric ion by the hydroxylamino moiety with the resulting ferrous ion being quantitated by coupling with 2,4,6-tripyridyl-s-triazine to form a purple color with a maximum absorbance at 595 nm. The method is specific to primary and secondary hydroxylamino compounds and calibration curves can be obtained in the range of concentrations of 1 to 20 μg/ml. Hydroxamic acid, amido, amino, phenolic, nitro, nitroso, oxime, nitrone, aldehyde, and ketone compounds do not produce any color reaction when analyzed by the same method. The method is simple, rapid, and many samples can be analyzed in a short period of time. 相似文献
6.
Toshinori Suzuki 《Nitric oxide》2007,16(2):266-273
Uric acid is a well-established scavenger of reactive oxygen and nitrogen species such as hydroxyl radical and peroxynitrite. However, little attention has been paid to the relationship between uric acid and nitric oxide. This paper reports the identification and characterization of a reaction product of uric acid induced by nitric oxide. When uric acid was treated with nitric oxide gas in a neutral solution under aerobic conditions, uric acid was consumed, yielding an unknown product. The product was identified as nitrosated uric acid from mass spectrometric data, although the position of the nitroso group on the molecule was not determined. The nitrosated uric acid decomposed to several compounds including uric acid with a half-life of 2.2 min at pH 7.4 and 37 degrees C. The incubation of nitrosated uric acid with glutathione resulted in the formation of S-nitrosoglutathione. Nitrosated uric acid was also formed in the reaction with nitric oxide donors, but not with peroxynitrite. Nitrosated uric acid was detected in human serum and urine by in vitro treatment with a nitric oxide donor. In the reaction of glutathione with the nitric oxide donor, the addition of uric acid caused an increase in the yield of S-nitrosoglutathione. These results indicate that under aerobic conditions nitric oxide can convert uric acid into its nitroso derivative, which can give a nitroso group to glutathione. Uric acid may act as a vehicle of nitric oxide in humans. 相似文献
7.
Dimethyl carbonate as potential reactant in non-catalytic biodiesel production by supercritical method 总被引:2,自引:0,他引:2
In this study, the non-catalytic supercritical method has been studied in utilizing dimethyl carbonate. It was demonstrated that, the supercritical dimethyl carbonate process without any catalysts applied, converted triglycerides to fatty acid methyl esters with glycerol carbonate and citramalic acid as by-products, while free fatty acids were converted to fatty acid methyl esters with glyoxal. After 12 min of reaction at 350 degrees C/20 MPa, rapeseed oil treated with supercritical dimethyl carbonate reached 94% (w/w) yield of fatty acid methyl ester. The by-products from this process which are glycerol carbonate and citramalic acid are much higher in value than glycerol produced by the conventional process. In addition, the yield of the fatty acid methyl esters as biodiesel was almost at par with supercritical methanol method. Therefore, supercritical dimethyl carbonate process can be a good candidate as an alternative biodiesel production process. 相似文献
8.
By the use of EPR spectroscopy, it has been shown that acyl nitroso compounds can act as spin traps for short-lived radicals with the formation of acyl aminoxyl radicals. The reaction was studied for the system benzohydroxamicacid[Ph-C (= O)N(H)] - dimethyl sulfoxide - hydrogen peroxide. The acyl aminoxyl radicals appeared almost immediately when the reaction mixture was irradiated in situ in the EPR cavity with UV light. The trapping reaction involved two photochemical reactions, i.e. the oxidation of the hydroxamic acid to the acyl nitroso compound Ph-C (= O)NO, and the formation of methyl radicals from dimethyl sulfoxide. The EPR spectra are superpositions of the spectra of two species of acyl aminoxyl radicals, i.e. the radicals Ph-C (= O)N(O·)H formed by oxidation of the parent benzohydrox-amic acid, and the radical Ph-C (= O)N(O·)CH3, formed by trapping of methyl radicals. 相似文献
9.
The oxidation mechanism of V(IV)/V(V) in the presence of N-hydroxyacetamide (acetohydroxamic acid, HL) in aqueous solution has been investigated using density functional theory (DFT) calculations aiming to contribute to the understanding of this process at a molecular level. The mechanism proposed involves formation of the *OH, *OOH, H2O2 radicals and complexes formed from the interaction of these species with VOL2 complex. The Gibbs free energy of each step of the mechanism has been evaluated. The solvation energy has been estimated by means of united atoms Hartree-Fock/polarizable continuum method (UAHF/PCM). The Gibbs free energy of the global reaction of the V(IV)/V(V) oxidation has been estimated and compared with the available experimental equilibrium constant. The difference between the calculated and experimental estimates for the reaction energy of the global equation is about 1.5 kcal mol(-1). The thermodynamic profile of the reaction mechanism has been provided and discussed in terms of the possible intermediates. The influence of the ligand and the reaction rate in terms of the steady-state approximation has been briefly discussed. 相似文献
10.
A method for the determination of acetic acid in presence of a large amount of sulfuric acid has been developed. The method consists of the following procedures. The sample is neutralyzed by barium carbonate. Barium sulfate and excess of barium carbonate are filtered off. On addition of sulfuric acid, acetic acid is extracted with n-butanol from the filtrate. By the reaction of acetic and sulfuric acids in butanol layer with aniline and furfural, a red color is produced. The color produced by sulfuric acid is bleached by treating with barium carbonate powder and the absorbancy of the color produced by acetic acid is measured in a photometer. Acetic acid determination by this method is disturbed by some other acids which give soluble barium salts but the acids which give insoluble barium salts do not disturb. 相似文献
11.
L. T. Chang E. L. McGrory R. P. Elander D. J. Hook 《Journal of industrial microbiology & biotechnology》1991,7(3):175-179
Summary Penicillin V (phenoxymethyl penicillin) is produced by industrial strains ofPenicillium chrysogenum in the presence of phenoxyacetic acid (POAc), a side-chain precursor for the penicillin V molecule. The wild-type strain ofP. chrysogenum produces an undesirable penicillin byproduct,para-hydroxypenicillin V (p-OH penicillin V), in addition to penicillin V, viapara-hydroxylation of POAc and subsequent incorporation of thep-OH phenoxyacetic acid into the penicillin molecule. Most of thep-OH penicillin V is produced late in cycle when the POAc concentration in the medium is nearly depleted. The level ofp-OH penicillin V produced by the control strain ranges up to 10–15% of the total penicillins produced. 3-Phenoxypropionic acid andp-bromophenylacetic acid partially inhibit the formation ofp-OH penicillin V with a minimal effect on penicillin V productivity. Mutants deficient in their ability to hydroxylate POAc were found to produce lower levels ofp-OH penicillin V. Multi-step mutation and screening, starting with the wild-type strain, have culminated in isolation of mutants which producep-OH penicillin V as 1% of the total penicillins with no adverse effect on penicillin V productivity. 相似文献
12.
Upon electrolytic reduction of a range of nitro-aromatic complexes (including imidazoles. benzenoids. furans and pyrazoles) an associated oxidation-reduction process is observed at more positive potentials with respect to nitro group reduction when using repeat scan cyclic voltammetry. This new couple has been identified as the reversible first reduction of the nitroso derivative for chloramphenicol, by the addition of a genuine sample of nitrosochloramphenicol to the electrochemical cell. We have failed to observe formation of the new redox-active species for five 5-nitroimidazoles examined.
Possible reaction schemes for nitroso formation under electrolytic reduction conditions and the importance of the nitroso redox couple with respect to the cytotoxic action of the parent drug are discussed. The applicability of nitrosochloramphenicol as a model for the behaviour of nitroso-heterocycles in general is shown. 相似文献
Possible reaction schemes for nitroso formation under electrolytic reduction conditions and the importance of the nitroso redox couple with respect to the cytotoxic action of the parent drug are discussed. The applicability of nitrosochloramphenicol as a model for the behaviour of nitroso-heterocycles in general is shown. 相似文献
13.
A new and efficient method for the preparation in one step of water-soluble cellulose acetate sulfate derivatives (CAS) is reported. Acetylation and sulfation were carried out simultaneously, using a mixture of acetic anhydride and sulfuric acid in glacial acetic acid. The reaction time and the amount of acetic anhydride were optimized and the method provided water-soluble esters, with a degree of acetylation in the range 1.6 and 2.4 and a degree of sulfation of 0.3. This method has been successfully applied to pure cellulose and to cellulose-enriched materials obtained from agricultural by-products. The product exhibited a high viscosity in aqueous solution suggesting interesting rheological properties. 相似文献
14.
Uchida K Kudo T Suzuki KI Nakase T 《The Journal of General and Applied Microbiology》1999,45(2):49-56
The glycolate test is a method to discriminate N-acyl groups of muramyl residue in peptidoglycan of bacterial cell walls by color reaction without purification of the cell walls. The glycolyl residue presents red purple color by heating with 0.02% 2,7-dihydroxynaphthalene (DON) dissolved in concentrated H(2)SO(4). Instead of the previous column methods for quantitative analysis, a qualitative method by solvent works was developed to simplify and to miniaturize the analysis. In this method, solvents played two roles, removal of interfering materials and extraction of glycolic acid from the cell hydrolysates. Of several solvent systems tested, diethyl ether was studied in detail on such properties as the efficiency of glycolic acid extraction under several conditions, the ability of removing various interfering compounds, and the advantage on evaporation procedure of the solvent from extracts. DON reaction of the second diethyl ether extract from cell hydrolysate of "Micromonospora nigra" JCM 3328 showed a clear red purple color of a strong absorbance at 530 nm, which is the same as that of authentic glycolic acid. The solvent method was applied to 20 strains of typical actinomycete species whose acyl types have already been known (Uchida and Seino, 1997). All glycolate test positive strains showed the clear red purple color mentioned above, whereas acetyl type strains revealed no apparent color by the same procedures. Additional experiments indicated that the glycolate test could be determined with less than 1 mg of actinomycete cells by using a smaller amount of DON reagent and ordinary polypropylene tubes. The new method was discussed for advantages in the identification of actinomycetes and for possible applications to other fields. 相似文献
15.
《Free radical research》2013,47(6):327-332
Upon electrolytic reduction of a range of nitro-aromatic complexes (including imidazoles. benzenoids. furans and pyrazoles) an associated oxidation-reduction process is observed at more positive potentials with respect to nitro group reduction when using repeat scan cyclic voltammetry. This new couple has been identified as the reversible first reduction of the nitroso derivative for chloramphenicol, by the addition of a genuine sample of nitrosochloramphenicol to the electrochemical cell. We have failed to observe formation of the new redox-active species for five 5–nitroimidazoles examined.Possible reaction schemes for nitroso formation under electrolytic reduction conditions and the importance of the nitroso redox couple with respect to the cytotoxic action of the parent drug are discussed. The applicability of nitrosochloramphenicol as a model for the behaviour of nitroso-heterocycles in general is shown. 相似文献
16.
Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(6):689-706
Some factors affecting the skatole-hydrochloric acid reaction for fructose were studied. Especially, the stability of the chromogen to light, the effect of the amount of chloroform for complete extraction of the chromogen from the aqueous phase, and the time course of color development at various temperatures were studied in some detail. The time course of color development in the β-indolylacetic acid-hydrochloric acid reaction for fructose was also investigated. Based on the results obtained from these observations, some modifications to both the skatole-hydrochloric acid and β-indolylacetic acid-hydrochloric acid reactions were proposed.A modification of the resorcinol-thiourea-hydrochloric acid method of Roe et al.7) for the determination of fructose is described. The modification is based on the observation that by lowering the incubation temperature to 70°C, a greater color intensity ratio (fructose color/glucose color) is obtained, thus increasing the specificity of the method for fructose. By applying the principle of the two-point determination of Mokrasch12) to the modified procedure, fructose in the presence of 100-fold excess of glucose can be determined with an error of about 10%.A modified procedure of the cysteine-carbazole reaction for the determination of fructose is described. By incubating the components of the color reaction at 40°C for 1 hr, fructose is determined with good sensitivity (the millimolar absorbance value of 29.3) and specificity (the color intensity ratio of fructose to glucose is about 240). When the principle of the two-point determination is applied to this modified procedure (1 hr and 3 hr at 40°C), fructose as small an amount as 0.4 μg in the presence of 250-fold excess of glucose can be determined with an error of about 10 %. 相似文献
17.
Torres-Guzmán R de la Mata I Torres-Bacete J Arroyo M Castillón MP Acebal C 《Biochemical and biophysical research communications》2002,291(3):593-597
The kinetic parameters of several substrates of penicillin acylase from Streptomyces lavendulae have been determined. The enzyme hydrolyses phenoxymethyl penicillin (penicillin V) and other penicillins with aliphatic acyl-chains such as penicillin F, dihydroF, and K. The best substrate was penicillin K (octanoyl penicillin) with a k(cat)/K(m) of 165.3 mM(-1) s(-1). The enzyme hydrolyses also chromogenic substrates as NIPOAB (2-nitro-5-phenoxyacetamido benzoic acid), NIHAB (2-nitro-5-hexanoylamido benzoic acid) or NIOAB (2-nitro-5-octanoylamido benzoic acid), however failed to hydrolyse phenylacetil penicillin (penicillin G) or NIPAB (2-nitro-5-phenylacetamido benzoic acid) and penicillins with polar substituents in the acyl moiety. These results suggest that the structure of the acyl moiety of the substrate is more determinant than the amino moiety for enzyme specificity. The enzyme was inhibited by several organic acids and the extent of inhibition changed with the hydrophobicity of the acid. The best inhibitor was octanoic acid with a K(i) of 0.8 mM. All the results, taking together, point to an active site highly hydrophobic for this penicillin acylase from Streptomyces lavendulae. 相似文献
18.
The kinetics of oxidation of some aldoses by vanadium(V) in perchloric acid media have been investigated. Each reaction is first order with respect to both [Vanadium(V)] and [Aldose]. The reactions are catalysed by acid. The addition of sodium perchlorate accelerates the rate of reaction. Kinetic evidence for the formation of an intermediate compound between vanadium(V) and aldoses is insignificant, and a mechanism is suggested in which vanadium(V) reacts with the aldoses by a fast step to form a transition state, followed by the decomposition of the latter to give the products of reaction in a slow step. The formation of free-radical intermediates has been demonstrated, and one-electron reduction of vanadium(V) by aldoses seems to be the most plausible mechanism. The oxidation rates follow the order: xyloses arabinose galactose mannose. The activation parameters are reported. 相似文献
19.
Jian Zhang 《AAPS PharmSciTech》2015,16(6):1495-1499
A color reaction of aspirin with ferrous gluconate was studied by UV-Vis spectrophotometry and HPLC-MS. It was found that the UV-Vis spectra of the two drugs were different before and after they were mixed in water at about 0.3 M (diluted by >20 times for analysis), indicating that a complexation reaction took place. The drug-iron complex dissociated when the reacting solution was diluted by 400 times. The by-products of the reaction identified by HPLC-MS were salicylic acid, acetylated gluconic acid, salicylate-gluconic acid conjugate, and an oxidized product of salicylic acid that was complexed with iron with a molecular weight of 212. This reaction may be used as an important consideration to optimize the dosing regime of the two drugs and to help explain some pharmacological reactions between aspirin and biomolecules.KEY WORDS: aspirin, ferrous gluconate, reaction 相似文献
20.
Hydrolysis of penicillins and related compounds by the cell-bound penicillin acylase of Escherichia coli 总被引:9,自引:4,他引:5
M. Cole 《The Biochemical journal》1969,115(4):733-739
1. A method is given for the preparation of penicillin acylase by using Escherichia coli N.C.I.B. 8743 and a strain selected for higher yield. The enzyme is associated with the bacterial cells and removes the side chains of penicillins to give 6-amino-penicillanic acid and a carboxylic acid. 2. The rates of penicillin deacylation indicated that p-hydroxybenzylpenicillin was the best substrate, followed in diminishing order by benzyl-, dl-alpha-hydroxybenzyl-, 2-furylmethyl-, 2-thienylmethyl-, d-alpha-aminobenzyl-, n-propoxymethyl- and isobutoxymethyl-penicillin. Phenylpenicillin and dl-alpha-carboxybenzylpenicillin were not substrates and phenoxymethyl-penicillin was very poor. 3. Amides and esters of the above penicillins were also substrates for the deacylation reaction, as were cephalosporins with a thienylmethyl side chain. 4. For the deacylation of 2-furylmethylpenicillin at 21 degrees the optimum pH was 8.2. The optimum temperature was 60 degrees at pH7. 5. By using selection A of N.C.I.B. 8743 and determining reaction velocities by assaying yields of 6-amino-penicillanic acid in a 10min. reaction at 50 degrees and pH8.2, the K(m) for benzylpenicillin was found to be about 30mm and the K(m) for 2-furylmethylpenicillin, about 10mm. The V(max.) values were 0.6 and 0.24mumole/min./mg. of bacterial cells respectively. 相似文献