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1.
Cytotoxic compounds from the marine actinobacterium Streptomyces corchorusii AUBN1/71 总被引:1,自引:0,他引:1
G. Adinarayana M. R. Venkateshan V. V. S. N. K. Bapiraju P. Sujatha J. Premkumar P. Ellaiah A. Zeeck 《Russian Journal of Bioorganic Chemistry》2006,32(3):295-300
We isolated a bioactive streptomycete from marine sediment samples collected at Bay of Bengal, India, during our systematic
study of marine actinobacteria. The taxonomic studies indicated that the isolate is related to Strepomyces corchorusii. However, it differed in certain aspects, and, hence, was designated as S. corchorusii AUBN1/7. A solvent extraction followed by a chromatographic purification helped obtain from the isolate two cytotoxic compounds,
which were identified as resistomycin, a quinone-related antibiotic, and tetracenomycin D, an anthraquinone antibiotic, on
the basis of spectral data of pure compounds. They demonstrated in vitro a potent cytotoxic activity against cell lines HMO2
(gastric adenocarcinoma) and HePG2 (hepatic carcinoma) and also exhibited weak antibacterial activities against Gram-positive
and Gram-negative bacteria.
Published in Russian in Bioorganicheskaya Khimiya, 2006, Vol. 32, No. 3, pp. 328–334.
The text was submitted by the authors in English. 相似文献
2.
Marine sediment samples from Visakhapatnam coast of Bay of Bengal, India, were investigated as a source of actinomycetes to screen for the production of antibiotics and cytotoxic compounds. Actinomycete strain DVR D4 with interesting bioactivity profile was isolated during our systematic study of marine actinomycetes. Based on biochemical properties and 16S rDNA analysis the isolate DVR D4 was identified as a strain of Amycolatopsis alba. A solvent extraction followed by a chromatographic purification helped to isolate a cytotoxic compound, which was identified as 1(10-aminodecyl) Pyridinium salt antibiotic, on the basis of spectral data. The compound showed potent cytotoxic activity against cancer cell lines of cervix (HeLa), breast (MCF-7) and brain (U87MG) in vitro and also exhibited antibacterial activities against Gram-positive and Gram-negative bacteria. 相似文献
3.
Gorajana A Venkatesan M Vinjamuri S Kurada BV Peela S Jangam P Poluri E Zeeck A 《Microbiological research》2007,162(4):322-327
In our systematic screening programme for marine actinomycetes, a bioactive Streptomycete was isolated from marine sediment samples of Bay of Bengal, India. The taxonomic studies indicated that the isolate belongs to Streptomyces chibaensis and it was designated as S. chibaensis AUBN1/7. The isolate yielded a cytotoxic compound. It was obtained by solvent extraction followed by the chromatographic purification. Based on the spectral data of the pure compound, it was identified as quinone-related antibiotic, resistoflavine (1). It showed a potent cytotoxic activity against cell lines viz. HMO2 (Gastric adenocarcinoma) and HePG2 (Hepatic carcinoma) in vitro and also exhibited weak antibacterial activities against Gram-positive and Gram-negative bacteria. 相似文献
4.
Red to red - the marine bacterium Hahella chejuensis and its product prodigiosin for mitigation of harmful algal blooms 总被引:2,自引:0,他引:2
Kim D Kim JF Yim JH Kwon SK Lee CH Lee HK 《Journal of microbiology and biotechnology》2008,18(10):1621-1629
Harmful algal blooms (HABs), commonly called red tides, are caused by some toxic phytoplanktons, and have made massive economic losses as well as marine environmental disturbances. As an effective and environment-friendly strategy to control HAB outbreaks, biological methods using marine bacteria capable of killing the harmful algae or algicidal extracellular compounds from them have been given attention. A new member of the gamma-Proteobacteria, Hahella chejuensis KCTC 2396, was originally isolated from the Korean seashore for its ability to secrete industrially useful polysaccharides, and was characterized to produce a red pigment. This pigment later was identified as an alkaloid compound, prodigiosin. During the past several decades, prodigiosin has been extensively studied for its medical potential as immunosuppressants and antitumor agents, owing to its antibiotic and cytotoxic activities. The lytic activity of this marvelous molecule against Cochlodinium polykrikoides cells at very low concentrations (1 ppb) was serendipitously detected, making H. chejuensis a strong candidate among the biological agents for HAB control. This review provides a brief overview of algicidal marine bacteria and their products, and describes in detail the algicidal characteristics, biosynthetic process, and genetic regulation of prodigiosin as a model among the compounds active against red-tide organisms from the biochemical and genetic viewpoints. 相似文献
5.
A new compound (1), named diaporthelactone, together with two known compounds (2 and 3) were isolated from the culture of Diaporthe sp., a marine fungus growing in the submerged rotten leaves of Kandelia candel in the mangrove nature conservation areas of Fugong, Fujian Province of China. The new compound was elucidated to be 1,3-dihydro-4-methoxy-7-methyl-3-oxo-5-isobenzofuran-carboxyaldehyde (1), which showed cytotoxic activity against KB and Raji cell lines (IC50 6.25 and 5.51 microg mL(-1), respectively). Two known compounds, 7-methoxy-4,6-dimethyl-3H-isobenzofuran-1-one (2) and mycoepoxydiene (3), were also demonstrated to exhibit cytotoxic activities for the first time. All three compounds were assessed for antimicrobial activity. 相似文献
6.
Elmutasim O. Ibnouf 《Saudi Journal of Biological Sciences》2021,28(8):4619-4625
Actinomycetes have been identified as an origin of many secondary metabolites, antibiotics and active components that impact microbial growth. Mediated mutations using UV in practice for the breeding of organisms. The objective of this study is to analyses the impact of UV radiation on the (O-7) Actinomycete isolate. This was a prospective analytical study of a several of actinomycetes. The isolates were screened for antimicrobial efficacy against multiple Gram-positive, Gram-negative bacteria, yeast, and fungi. Various factors such as UV, temperature, pH, light, agitation, fermentation durations and aeration have also been boosted for optimal antimicrobial production. The isolate (O-7) Actinomycete has been recognized as a highly bioactive producing organism. The isolate was exposed to various wavelengths, times under numerous growth conditions. It was found that 4% concentration of glucose as a carbon source is significantly optimal for the production of antibiotic for (O-7) UV exposed strain, however, concentration of 1% of lactose is significantly optimal for the production of antibiotic for (O-7) UV exposed strain. Yeast extract at a concentration of 1% was found to be the best source of nitrogen for (O-7) UV exposed, while pH 7.0 was found to be the most suitable for the same isolate. From the temperature optimization study, it was observed that (O-7) exposed strain showed good growth and maximum antibiotic production at 28 °C. The soil-isolated biological compounds (O-7) were effective against certain types of bacteria and fungi, and the research also demonstrated that exposure to UV radiation enhanced the production of these compounds. 相似文献
7.
为从广西北部湾的泥样和植物中分离海洋放线菌,筛选具有抑菌活性的菌株,分离活性化合物。研究采用普通稀释法分离菌株,对发酵产物进行抑菌活性测试,利用活性追踪分离活性化合物,并通过波谱方法确定化合物结构。结果表明从6个海泥样品和3个植物样品中共分离73株放线菌,筛选得到具有抑香蕉枯萎病和金黄色葡萄球菌活性的菌株7株,并从其中的1株链霉菌 Streptomyces sp.MDCW-126的次级代谢产物中分离鉴定了星形孢菌素。从广西北部湾分离的药用活性菌株资源具有开发和深入研究价值。 相似文献
8.
One solution to the global crisis of antibiotic resistance is the discovery of novel antimicrobial compounds for clinical
application. Marine organisms are an attractive and, as yet, relatively untapped resource of new natural products. Cell extracts
from the marine diatom, Phaeodactylum tricornutum, have antibacterial activity and the fatty acid, eicosapentaenoic acid (EPA), has been identified as one compound responsible
for this activity. During the isolation of EPA, it became apparent that the extracts contained further antibacterial compounds.
The present study was undertaken to isolate these additional antibacterial factors using silica column chromatography and
reverse-phase high-performance liquid chromatography. Two antibacterial fractions, each containing a pure compound, were isolated
and their chemical structures were investigated by mass spectrometry and nuclear magnetic resonance spectroscopy. The antibacterial
compounds were identified as the monounsaturated fatty acid (9Z)-hexadecenoic acid (palmitoleic acid; C16:1 n-7) and the relatively unusual polyunsaturated fatty acid (6Z, 9Z, 12Z)-hexadecatrienoic acid (HTA; C16:3 n-4). Both are active against Gram-positive bacteria with HTA further inhibitory to the
growth of the Gram-negative marine pathogen, Listonella anguillarum. Palmitoleic acid is active at micro-molar concentrations, kills bacteria rapidly, and is highly active against multidrug-resistant
Staphylococcus aureus. These free fatty acids warrant further investigation as a new potential therapy for drug-resistant infections. 相似文献
9.
目的:药用植物内生真菌是一类重要的微生物资源,其代谢产物有着广泛的生物学活性。该研究拟从野葛(Pueraria lobata(Willd.)Ohwi)中分离有细胞毒活性的内生真菌。方法:组织块改良法分离内生真菌;细胞毒活性测定采用Alamar blue法;结合形态学和分子生物学方法进行菌种鉴定。结果:获得的34株内生真菌中菌株KLBMP f0027对HepG2、HO-8910、NCI-H460、SGC-7901等细胞株均有显著活性,ID50分别为437.4、460.0、542.5、771.2。而且活性产物相对稳定,对温度、pH变化及蛋白酶不敏感。代谢过程研究显示菌株KLBMP f0027的活性产物合成与细胞生长属于部分耦联关系类型。形态学和ITS-rDNA序列分析鉴定菌株为Aspergillus ostianus strain KLBMP f0027。结论:野葛内生真菌A.ostianus strain KLBMP f0027可作为细胞毒活性候选菌株进行深入研究。 相似文献
10.
Many marine bacteria demonstrate antibiotic activity against organisms of terrestrial origin. Low molecular weight antibiotics have been extracted and, in some cases, purified, but few attempts have been made to isolate high molecular weight antibiotics produced by marine bacteria. In the study reported here, a high molecular weight antibiotic was extracted from whole cells ofAlteromonas strain P18 (NCMB 1890) grown on 2216E medium. Purification included ammonium sulfate precipitation, ultracentrifugation, chromatography on DEAE cellulose, and gel filtration on Ultrogel. A rapid method for measuring specific activity of the antibiotic was developed. 相似文献
11.
Aims: The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, and to investigate the apoptosis effects of its metabolite on colon cancer cells.
Method and Results: We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic activity on three colon cancer cells (HT-29, SW480 and HCT116). Biochemical tests and 16S rDNA sequencing of this strain allowed us to identify BIT-33 as a strain of Bacillus vallismortis . The cytotoxic compound from B. vallismortis BIT-33 was purified by reverse-phase high-performance liquid chromatography. Direct cytotoxic effect of the compound was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide assay. The compound induced apoptosis of colon cancer cells, as indicated by DNA fragmentation of agarose gel electrophoresis, flow cytometric analysis (sub-G1 method) and annexin V staining.
Conclusion: The cytotoxic compound from B. vallismortis BIT-33 was purified, and the compound showed direct cytotoxic and apoptotic effects on colon cancer cells in a dose- and time-dependent manner.
Significance and Impact of the Study: Taken together, our results suggest that the compound from B. vallismortis BIT-33 could be a candidate for the development of apoptosis-specific anti-tumour agents. This study indicated that marine bacteria could be an important source of cytotoxic metabolites. 相似文献
Method and Results: We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic activity on three colon cancer cells (HT-29, SW480 and HCT116). Biochemical tests and 16S rDNA sequencing of this strain allowed us to identify BIT-33 as a strain of Bacillus vallismortis . The cytotoxic compound from B. vallismortis BIT-33 was purified by reverse-phase high-performance liquid chromatography. Direct cytotoxic effect of the compound was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide assay. The compound induced apoptosis of colon cancer cells, as indicated by DNA fragmentation of agarose gel electrophoresis, flow cytometric analysis (sub-G
Conclusion: The cytotoxic compound from B. vallismortis BIT-33 was purified, and the compound showed direct cytotoxic and apoptotic effects on colon cancer cells in a dose- and time-dependent manner.
Significance and Impact of the Study: Taken together, our results suggest that the compound from B. vallismortis BIT-33 could be a candidate for the development of apoptosis-specific anti-tumour agents. This study indicated that marine bacteria could be an important source of cytotoxic metabolites. 相似文献
12.
Brahic C Darro F Belloir M Bastide J Kiss R Delfourne E 《Bioorganic & medicinal chemistry》2002,10(9):2845-2853
4-Substituted-7H-pyrido-[4,3,2-de][1,8] or [1,9]-phenanthroline-7-ones and 9-methyl-1,4-diazanaphtacene-3,10-dione, analogues of the marine pyridoacridine amphimedine were synthesised from isoquinoline-5,8-dione. The first compounds were obtained starting from a Diels-Alder reaction whereas the synthesis of the last compound was initiated by a reaction of condensation with 2-aminoacetophenone. The different tetra- and pentacyclic compounds were evaluated for in vitro cytotoxic activities against six distinct human cancer cell lines. All the compounds exhibit cytotoxic activity with IC(50) values (i.e., the drug concentration inhibiting the mean growth value of the six cell lines by 50%)<10(-7)M for two of them. 相似文献
13.
José A. Gutiérrez-Barranquero F. Jerry Reen María L. Parages Ronan McCarthy Alan D. W. Dobson Fergal O'Gara 《Microbial biotechnology》2019,12(5):1049-1063
In recent years, the marine environment has been the subject of increasing attention from biotechnological and pharmaceutical industries. A combination of unique physicochemical properties and spatial niche-specific substrates, in wide-ranging and extreme habitats, underscores the potential of the marine environment to deliver on functionally novel bioactivities. One such area of ongoing research is the discovery of compounds that interfere with the cell–cell signalling process called quorum sensing (QS). Described as the next generation of antimicrobials, these compounds can target virulence and persistence of clinically relevant pathogens, independent of any growth-limiting effects. Marine sponges are a rich source of microbial diversity, with dynamic populations in a symbiotic relationship. In this study, we have harnessed the QS inhibition (QSI) potential of marine sponge microbiota and through culture-based discovery have uncovered small molecule signal mimics that neutralize virulence phenotypes in clinical pathogens. This study describes for the first time a marine sponge Psychrobacter sp. isolate B98C22 that blocks QS signalling, while also reporting dual QS/QSI activity in the Pseudoalteromonas sp. J10 and ParacoccusJM45. Isolation of novel QSI activities has significant potential for future therapeutic development, of particular relevance in the light of the pending perfect storm of antibiotic resistance meeting antibiotic drug discovery decline. 相似文献
14.
Surface Attachment Induced Production of Antimicrobial Compounds by Marine Epiphytic Bacteria Using Modified Roller Bottle Cultivation 总被引:1,自引:0,他引:1
A modified roller bottle culture method elicited the production of antimicrobial compounds from 2 epibiotic marine bacterial
strains, EI-34-6 and II-111-5, isolated from the surface of the marine alga Palmaria palmata. These isolates, tentatively
identified as Bacillus species, were grown as a biofilm on the surface of nutrient glycerol ferric agar (NGFA) and marine
Columbia glycerol agar (MCGA) on the inside of a rolling bottle. The biofilm was shown to be stable, and the cells were difficult
to remove from the agar surface. The culture supernatant exhibited a different antibiotic spectrum when the strains were grown
using the agar roller bottle method compared with shake flask cultures or nonagar roller bottle cultures. These results suggest
that biofilm formation is an important factor in the production of antimicrobial compounds by these 2 strains, and roller
bottle cultivation also allowed production of these compounds to be increased. The methodology used here has the potential
to allow increased production of useful secondary metabolites such as antibiotics from marine epibiotic bacteria. 相似文献
15.
Manmadhan Kanagasabhapathy Shinichi Nagata 《World journal of microbiology & biotechnology》2008,24(5):687-691
Four antibiotic producing bacteria were isolated from the surface of the marine sponge Pseudoceratina purpurea and exposed to living cells of two human pathogenic bacteria as well as some marine fouling bacteria to induce the production
of antimicrobial activity. Experimental results showed that these four marine epibiotic bacteria enhanced their antibacterial
production, when exposed to these test strains. The highest induction was exhibited by the sponge isolate PS79 against fouling
bacterium FB-9 (from 3 mm to 7 mm inhibition zone). All the four strains were induced and showed increased activity specifically
against the challenged pathogenic or fouling bacteria tested. Specific induction by these species suggests that the induction
might be attributed to the response to the chemical signals received from potential challenger strains. 相似文献
16.
O Jansen M Tits L Angenot JP Nicolas P De Mol JB Nikiema M Frédérich 《Malaria journal》2012,11(1):289
ABSTRACT: BACKGROUND: Natural products could play an important role in the challenge to discover new anti-malarial drugs. In a previous study, Dicoma tomentosa (Asteraceae) was selected for its promising anti-plasmodial activity after a preliminary screening of several plants traditionally used in Burkina Faso to treat malaria. The aim of the present study was to further investigate the antiplasmodial properties of this plant and to isolate the active anti-plasmodial compounds. METHODS: Eight crude extracts obtained from D. tomentosa whole plant were tested in vitro against two Plasmodium falciparum strains (3D7 and W2) using the p-LDH assay (colorimetric method). The Peters' four-days suppressive test model (Plasmodium berghei-infected mice) was used to evaluate the in vivo anti-plasmodial activity. An in vitro bioguided fractionation was undertaken on a dichloromethane extract, using preparative HPLC and TLC techniques. The identity of the pure compound was assessed using UV, MS and NMR spectroscopic analysis. In vitro cytotoxicity against WI38 human fibroblasts (WST-1 assay) and haemolytic activity were also evaluated for extracts and pure compounds in order to check selectivity. RESULTS: The best in vitro anti-plasmodial results were obtained with the dichloromethane, diethylether, ethylacetate and methanol extracts, which exhibited a high activity (IC50 [less than or equal to] 5 mug/ml). Hot water and hydroethanolic extracts also showed a good activity (IC50 [less than or equal to] 15 mug/ml), which confirmed the traditional use and the promising anti-malarial potential of the plant. The activity was also confirmed in vivo for all tested extracts. However, most of the active extracts also exhibited cytotoxic activity, but no extract was found to display any haemolytic activity. The bioguided fractionation process allowed to isolate and identify a sesquiterpene lactone (urospermal A-15-O-acetate) as the major anti-plasmodial compound of the plant (IC50 < 1 mug/ml against both 3D7 and W2 strains). This was also found to be the main cytotoxic compound (SI =3.3). While this melampolide has already been described in the plant, this paper is the first report on the biological properties of this compound. CONCLUSIONS: The present study highlighted the very promising anti-plasmodial activity of D. tomentosa and enabled to identify its main active compound, urospermal A-15-O-acetate. The high antiplasmodial activity of this compound merits further study about its anti-plasmodial mechanism of action. The active extracts of D. tomentosa, as well as urospermal A 15-Oacetate, displayed only a moderate selectivity, and further studies are needed to assess the safety of the use of the plant by the local population. 相似文献
17.
A new hirsutane sesquiterpenoid, hirsutanol E (1), together with the known compounds hirsutanol A (2) and hirsutanol F (3; gloeosteretriol) were obtained from AcOEt extract of the marine fungus Chondrostereum sp., which was isolated from the soft coral Sarcophyton tortuosum. The structures were elucidated mainly on the basis of the NMR, MS, and X-ray single-crystal diffraction data. Hirsutanol A (2) exhibited potent cytotoxic activities against various cancer cell lines. 相似文献
18.
Govindam SV Choi BK Yoshioka Y Kanamoto A Fujiwara T Okamoto T Ojika M 《Bioscience, biotechnology, and biochemistry》2012,76(5):999-1002
A library of extracts established from hundreds of marine organisms was screened by a cytotoxicity test. The active organic extract of an Okinawan marine sponge of the genus Dysidea was subjected to bioassay-guided fractionation to give three new polyoxygenated steroids dysideasterols F-H (1-3), together with two known related compounds (4 and 5). Their structures were confirmed by NMR and mass spectroscopic analyses. A characteristic structural feature of 2, 4 and 5 is an allylic epoxide, whereas this epoxide undergoes ring-opening by a neighbouring hydroxyl group to give a tetrahydrofuran ring in 1 and 3. All compounds 1-5 exhibited a similar cytotoxic effect with IC50 values of 0.15-0.3 μM against human epidermoid carcinoma A431 cells, demonstrating that the allylic epoxide moiety was not responsible for this cytotoxic effect. 相似文献
19.
Nodularia harveyana, a dinitrogen-fixing cyanobacterial isolate from the Mediteranean Sea, grown in an outdoor photobioreactor,
was assayed for its bioactive compounds. The active substance(s) were lypophilic and showed strong allelopathic actvity against
other axenic cyanobacteria, antibiotic activity against Gram-positive pathogenic bacteria and antifungal activity against
two plant pathogens. The extracts were toxic (LC50 at 30 μL) for grazers such as a rotifer (Brachionus calyciflorus) and a crustacean (Thamnocephalus platyurus).
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
20.
Three novel compounds with spiro-5, 6-lactone ring skeleton has been isolated from the fermentation broth of Massrison sp. which could be isolated repeatedly from wild Rehmannia glutinosa. Psetariae oryza P-2b was applied to guide fractionation of bioactive compounds produced by Massrison sp. The molecular structures were established by a variety of one- and two-dimensional NMR experiments and the compounds with similar skeleton were reported for the first time from endophytic fungi of terraneous plant. Antifungal and cytotoxic activities of the compounds were tested, compounds 2 and 3 displayed stronger antifungal and cytotoxic activities. The compounds have the potential to be antibiotic against fungal pathogens and tumor cells. 相似文献