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1.
Taxonomic studies were performed on three strains isolated from Cheonho reservoir in Cheonan, Korea. The isolates were Gram-negative,
aerobic, rod-shaped, non-motile, catalase-positive, and oxidase-positive. Colonies on solid media were cream-yellow, smooth,
shiny, and circular. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belong to the genus
Flavobacterium. The strains shared 98.6–99.4% sequence similarity with each other and showed less than 97% similarity with members of the
genus Flavobacterium with validly published names. The DNA-DNA hybridization results confirmed the separate genomic status of strains ARSA-42 T, ARSA-103 T, and ARSA-108 T. The isolates contained menaqui-none-6 as the predominant menaquinone and iso-C 15:0, iso-C 15:0 3-OH, iso-Ci 15:1 G, and iso-C 16:0 3-OH as the major fatty acids. The genomic DNA G+C content of the isolates were 31.4–33.2 mol%. According to the phenotypic
and genotypic data, these organisms are classified as representative of three novel species in the genus Flavobacterium, and the name Flavobacterium koreense sp. nov. (strain ARSA-42 T =KCTC 23182 T =JCM 17066 T =KACC 14969 T), Flavobacterium chungnamense sp. nov. (strain ARSA-103 T =KCTC 23183 T =JCM 17068 T =KACC 14971 T), and Flavobacterium cheonanense sp. nov. (strain ARSA-108 T =KCTC 23184 T =JCM 17069 T =KACC 14972) are proposed. 相似文献
2.
A novel bacterial strain, TRM F109 T, was isolated from hypersaline habitat in Sichuan Province, China. It was a Gram-positive, aerobic, non-motile, halotolerant, filamentous bacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.4 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain TRM F109 T within the genus Isoptericola. The low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain TRM F109 T represents a novel species of the genus Isoptericola, for which the name Isoptericola salitolerans sp. nov. is proposed. The type strain is TRM F109 T (=JCM 15901 T=KCTC 19617 T). 相似文献
3.
Four strains, designated as C-2, C-17 T, C-39 T and Ch-15, were isolated from farmed rainbow trout samples showing clinical signs during an investigation for a fish-health screening study. The pairwise 16S rRNA gene sequence analysis showed that strain C-17 T shared the highest identity level of 98.1 % with the type strain of Chryseobacterium piscium LMG 23089 T while strains C-2, C-39 T and Ch-15 were closely related to Chryseobacterium balustinum DSM 16775 T with an identity level of 99.3 %. A polyphasic approach involving phenotypic, chemotaxonomic and genome-based analyses was employed to determine the taxonomic provenance of the strains. The overall genome relatedness indices including dDDH and ANI analyses confirmed that strains C-2, C-17 T, C-39 T and Ch-15 formed two novel species within the genus Chryseobacterium. Chemotaxonomic analyses showed that strains C-17 T and C-39 T have typical characteristics of the genus Chryseobacterium by having phosphatidylethanolamine in their polar lipid profile, MK-6 as only isoprenoid quinone and the presence of iso-C 15:0 as major fatty acid. The genome size and G + C content of the strains ranged between 4.4 and 5.0 Mb and 33.5 – 33.6 %, respectively. Comprehensive genome analyses revealed that the strains have antimicrobial resistance genes, prophages and horizontally acquired genes in addition to secondary metabolite-coding gene clusters. In conclusion, based on the polyphasic analyses conducted on the present study, strains C-17 T and C-39 T are representatives of two novel species within the genus Chryseobacterium, for which the names Chryseobacterium turcicum sp. nov. and Chryseobacterium muglaense sp. nov. with the type strains C-17 T (=JCM 34190 T = KCTC 82250 T) and C-39 T (=JCM 34191 T = KCTC 822251 T), respectively, are proposed. 相似文献
5.
A novel bacterial strain, designated ARSA-111 T, was isolated from a freshwater reservoir in Cheonan, Korea. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the isolate belonged to the genus Flavobacterium of phylum Bacteroidetes. The 16S rRNA gene sequence of strain ARSA-111 T showed a high degree of sequence similarity to those of Flavobacteium cheonanense KACC 14972 T (97.3%), F. aquatile JCM 20475 T (97.1%), and other type strains of the genus Flavobacterium (< 97.0%). The phylogenetic tree and network analysis (i.e. median-joining) based on 16S rRNA gene sequences showed that strain ARSA-111 T is most closely related to F. aquatile JCM 20475 T. DNA-DNA hybridization experiment revealed 70% of genomic relatedness among strain ARSA-111 T, F. aquatile JCM 20475 T and F. cheonanense KACC 14972 T. The isolate had iso-C 15:1, iso-C 15:0, and iso-C 15:0 3-OH as predominant cellular fatty acids and MK-6 as a predominant menaquinone. The genomic DNA G+C content of the isolate was 35.6 mol%. On the basis of these data, strain ARSA-111 T is considered to be a novel species of the genus Flavobacterium, for which the name Flavobacterium aquaticum sp. nov. is proposed. The type strain is strain ARSA-111 T (=KACC 14973 T =KCTC 23185 T = JCM 17070 T). 相似文献
6.
Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702 T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum Omega T is an obligate aerobic proteolytic microorganism from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles and mesophiles. Strain Z-1702 T ferments glucose, maltose, fructose, mannose, sorbose, galactose, cellobiose, N-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and pullulan. Strain Omega T is strictly proteolytic utilizing a range of proteins and peptones. The main polar lipid fatty acid in both strains is iso-C 15:0, while other major components are various C 16 and C 17 isomers. According to pairwise sequence alignments using BLAST Gracilimonas was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702 T and strain Omega T as two different genera in a deep-branching clade of the new family level within the order Balneolales with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702 T it was proposed to represent a novel genus and species Natronogracilivirga saccharolityca gen. nov., sp. nov. (= DSMZ 109061 T =JCM 32930 T =VKM B 3262 T). Furthermore, phylogenetic and phenotypic parameters of N. saccharolityca and C. proteinivorum gen. nov., sp. nov., strain Omega T (=JCM 31662 T, =UNIQEM U979 T), make it possible to include them into a new family with a proposed designation Cyclonatronaceae fam. nov.. 相似文献
7.
Bartonella is one of the noncore bacterial genera in the honey bee (Apis mellifera) gut. So far, only one species, Bartonella apis, has been described from the honey bee gut. Previous analyses based on the genomic information of isolates and metagenome-assembled genomes suggested the existence of multiple Bartonella species in the bee guts. Here, 10 strains were isolated and characterized from the gut of A. mellifera from Jilin Province, China. New isolates shared >95% 16S rRNA gene sequence similarity with other species of the genus Bartonella. Phylogenetic analysis revealed that new isolates clustered with other type strains of Bartonella, and the bee gut Bartonella could be classified into three clades. The in silico DDH and average nucleotide identity values between strains of different clusters from the honey bee gut are 29.1–32.5% and 87.6–89.3%, all below the recommended 70.0% and 95% cutoff points. Cells are Gram-staining-negative rods and can grow on the surface of Brain Heart Infusion agar plates supplemented with defibrinated sheep blood in an aerobic environment with 5% CO 2 at 35–37 °C. Strains from different species varied in both phenotypic and chemotaxonomic characterizations. Comparative genomic analysis indicated that B. choladocola had unique sets of genes encoding invasin, representing the potential for this species to both live as a gut symbiont and also as an erythrocytic pathogen. Thus, we propose two novel species Bartonella choladocola sp. nov. whose type strain is W8125 T(=JCM 35030 T = ACCC 62057 T), and Bartonella apihabitans sp. nov. whose type strain is W8097 T(=JCM 35029 T = ACCC 62056 T). 相似文献
8.
A novel Gram-staining positive, aerobic, rod-shaped, non-motile and yellow-pigmented actinobacterium, designated strain WY83T, was isolated from a marine sediment of Indian Ocean. Strain WY83T grew optimally at 30–35 °C, pH 7–8 and with 0–3% (w/v) NaCl. The predominant menaquinones were MK-10, MK-11 and MK-12, and the major fatty acids were C19:1 ω9c/C19:1 ω11c, anteiso-C15:0, C17:0 3OH, and iso-C16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The cell-wall peptidoglycan contained lysine as a diamino acid. The DNA G?+?C content was 72.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and ninety-two bacterial core genes indicated that strain WY83T formed an evolutionary lineage with Chryseoglobus frigidaquae JCM 14730T, Chryseoglobus indicus CTD02-10-2T, Yonghaparkia alkaliphila JCM 15138T, Microcella alkaliphila DSM 18851T and Microcella putealis DSM 19627T within the radiation enclosing members of the family Microbacteriaceae. All pairwise percentage of conserved proteins between strain WY83T and the closely related phylogenetic neighbors were greater than 65%. The average nucleotide identity and in silico DNA–DNA hybridization values were both below the thresholds used for the delineation of a new species. On the basis of the evidence presented, strains WY83T, Y. alkaliphila JCM 15138T, C. frigidaquae JCM 14730T, M. alkaliphila DSM 18851T and M. putealis DSM 19627T should belong to different species of the same genus. Strain WY83T represents a novel species of the genus Microcella, for which the name Microcella flavibacter sp. nov. is proposed. The type strain is WY83T (=?KCTC 39637T?=?MCCC 1A07099T). Furthermore, Chryseoglobus frigidaquae, Chryseoglobus indicus, and Yonghaparkia alkaliphila were reclassified as Microcella frigidaquae comb. nov., Microcella indica nom. nov., and Microcella alkalica nom. nov., respectively. 相似文献
9.
A novel Gram-stain positive, aerobic, short rod-shaped, non-motile bacterium, designated strain CHO1 T, was isolated from rhizosphere soil from a ginseng agriculture field. Strain CHO1 T was observed to form yellow colonies on R2A agar medium. The cell wall peptidoglycan was found to contain alanine, glycine, glutamic acid, d-ornithine and serine. The cell wall sugars were identified as galactose, mannose, rhamnose and ribose. Strain CHO1 T was found to contain MK-11, MK-12, MK-13 as the predominant menaquinones and anteiso-C 15:0, iso-C 16:0, and anteiso-C 17:0 as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, an unidentified phospholipid and three unidentified glycolipids were found to be present in strain CHO1 T. Based on 16S rRNA gene sequence analysis, strain CHO1 T was found to be closely related to Microbacterium mangrovi DSM 28240 T (97.81 % similarity), Microbacterium immunditiarum JCM 14034 T (97.45 %), Microbacterium oryzae JCM 16837 T (97.33 %) and Microbacterium ulmi KCTC 19363 T (97.10 %) and to other species of the genus Microbacterium. The DNA G+C content of CHO1 T was determined to be 70.1 mol %. The DNA–DNA hybridization values of CHO1 T with M. mangrovi DSM 28240 T, M. immunditiarum JCM 14034 T, M. oryzae JCM 16837 T and M. ulmi KCTC 19363 T were 46.7 ± 2, 32.4 ± 2, 32.0 ± 2 and 29.2 ± 2 %, respectively. On the basis of genotypic, phenotypic and phylogenetic properties, it is concluded that strain CHO1 T represents a novel species within the genus Microbacterium, for which the name Microbacterium rhizosphaerae sp. nov. is proposed. The type strain of M. rhizosphaerae is CHO1 T (= KEMB 7306-513 T = JCM 31396 T). 相似文献
10.
Two bacterial strains, P0211 T and P0213 T, were isolated from a sea cucumber culture pond in China. The strains were able to resist high copper levels. These two strains were characterized at the phenotypic, chemotaxonomic, and genomic level. They were completely different colors, but the 16S rRNA genes showed 99.30% similarity. Phylogenetic analysis based on the sequences of the 16S rRNA gene and five housekeeping genes ( dnaK, sucC, rpoB, gyrB, and rpoD) supported the inclusion of these strains within the genus Alteromonas, and the two isolated strains formed a group separated from the closest species Alteromonas aestuariivivens KCTC 52655 T. Genomic analyses, including average nucleotide identity (ANIb and ANIm), DNA–DNA hybridization (DDH), and the percentage of conserved proteins (POCP), clearly separated strains P0211 T and P0213 T from the other species within the genus Alteromonas with values below the thresholds for species delineation. The chemotaxonomic features (including fatty acid and polar lipid analysis) of strains P0211 T and P0213 T also confirmed their differentiation from the related taxa.The results demonstrated that strains P0211 T and P0213 T represented two novel species in the genus Alteromonas, for which we propose the names Alteromonas flava sp. nov., type strain P0211 T (= KCTC 62078 T = MCCC 1H00242 T), and Alteromonas facilis sp. nov., type strain P0213 T (= KCTC 62079 T = MCCC 1H00243 T). 相似文献
11.
Nine bacterial strains designated MT3-5-12 T, MT3-5-27, MTV1-9, S-DT1-15 T, S-DT1-34, MTV5-3 T, MTV4-17, MTV5-12 and MTV5-13 were isolated from the upper layer (1–5 cm in depth) of tidal flat sediment in Quanzhou Bay, China. The 16S rRNA gene of these strains shared maximum sequence similarities with Aestuariivivens insulae KCTC 42350 T of 94.9–97.1%. Phylogenetic analyses based on 16S rRNA gene sequences and 120 conserved concatenated proteins placed these strains in three novel phylogenetic clades affiliated to the genus Aestuariivivens of the family Flavobacteriaceae. Strains MT3-5-12 T, MT3-5-27 and MTV1-9 were phylogenetically close to A. insulae KCTC 42350 T. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains MT3-5-12 Tand MTV1-9 and A. insulae KCTC 42350 T were estimated to be 78.5-78.7% and 22.5%, respectively. Strains S-DT1-15 T and S-DT1-34 formed a distinctly separated clade from A. insulae KCTC 42350 T. The ANI and dDDH values between strains S-DT1-15 T and S-DT1-34 and A. insulae KCTC 42350 T were 76.3–76.4% and 20.4–20.5%, respectively. The other four strains MTV5-3 T, MTV4-17, MTV5-12 and MTV5-13, formed a third novel clade, distinctly separated from A. insulae KCTC 42350 T. The ANI and dDDH values between strains MTV5-3 T and MTV4-17 and A. insulae KCTC 42350 T were 74.7% and 19.1–19.2%, respectively. The phylogenetic analyses and whole genomic comparisons, combined with phenotypic and chemotaxonomic features, strongly supported the nine strains could be classified as three novel species within the genus Aestuariivivens, for which the names Aestuariivivens marinum sp. nov. MT3-5-12 T, Aestuariivivens sediminis sp. nov. S-DT1-15 T, and Aestuariivivens sediminicola sp. nov. MTV5-3 T are proposed. 相似文献
12.
A Gram-stain negative, aerobic, oxidase and catalase positive, non-flagellated, pink coloured bacterium with gliding motility, designated as strain UDD1 T was isolated from soil. The bacterium lacked flexirubin-type pigments. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain UDD1 T formed a lineage within the family Cytophagaceae of the phylum Bacteroidetes, and forms a distinct clade with type strains of the closely related genus Pontibacter with similarities of 91.36–93.62%. Strain UDD1 T contained MK-7 as the predominant menaquinone and summed feature 4 (iso-C 17:1 I and/or anteiso-C 17:1 B) and iso-C 15:0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified glycolipid. The DNA G+C content of strain UDD1 T was 49 mol%. On the basis of phenotypic, genotypic and phylogenetic analyses, the strain UDD1 T represents a novel species of a new genus in the family Cytophagaceae, for which the name Edaphorhabdus rosea gen. nov., sp. nov., is proposed. The type strain of Edaphorhabdus rosea is UDD1 T (=?KCTC 62117 T =?JCM 32366 T). The Digital Protologue Database Taxon number for strain UDD1 T is GA00058. 相似文献
13.
Bifidobacterium is one of the dominating bacterial genera in the honey bee gut, and they are the key degrader of diet polysaccharides for the host. Previous genomic analysis shows that they belong to separate phylogenetic clusters and exhibited different functional potentials in hemicellulose digestion. Here, three novel strains from the genus Bifidobacterium were isolated from the guts of the honey bee (Apis mellifera). Phylogenomic analysis showed that the isolates could be grouped into four phylogenetic clusters. The average nucleotide identity values between strains from different clusters are <95%, while strains in Cluster IV belong to the characterized species Bifidobacterium asteroides. Carbohydrate-active enzyme annotation confirmed that the metabolic capacity for carbohydrates varied between clusters of strains. Cells are Gram-positive rods; they grew both anaerobically and in a CO 2-enriched atmosphere. All strains grew at a temperature range of 20–42 °C, with optimum growth at 35 °C. The pH range for growth was 5–9. Strains from different phylogenetic clusters varied in multiple phenotypic and chemotaxonomic characterizations. Thus, we propose three novel species Bifidobacterium apousia sp. nov. whose type strain is W8102 T (=CGMCC 1.18893 T = JCM 34587 T), Bifidobacterium choladohabitans sp. nov., whose type strain is B14384H11 T (=CGMCC 1.18892 T = JCM 34586 T), and Bifidobacterium polysaccharolyticum sp. nov. whose type strain is W8117 T (=CGMCC 1.18894 T = JCM 34588 T). 相似文献
14.
A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK1-5 T, was isolated from the junction between the North Pacific Ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK1-5 T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DSSK1-5 T fell within the clade comprising Loktanella species, clustering consistently with the type strains of Loktanella hongkongensis and Loktanella cinnabarina, with which it exhibited 98.9 and 98.4 % sequence similarity values, respectively. Sequence similarities to the type strains of the other recognized Loktanella species were 94.0–96.2 %. Strain DSSK1-5 T was found to contain Q-10 as the predominant ubiquinone and C 18:1 ω7 c as the major fatty acid. The major polar lipids of strain DSSK1-5 T were identified as phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content of strain DSSK1-5 T was determined to be 67.6 mol% and its mean DNA–DNA relatedness values with L. hongkongensis JCM 12479 T and L. cinnabarina JCM 18161 T were 19 and 23 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain DSSK1-5 T is separated from other Loktanella species. On the basis of the data presented, strain DSSK1-5 T is proposed to represent a novel species of the genus Loktanella, for which the name Loktanella soesokkakensis sp. nov. is proposed. The type strain is DSSK1-5 T (= KCTC 32425 T = CECT 8367 T). 相似文献
16.
The taxonomic positions of five Gram-negative, non-spore-forming and non-motile bacterial strains isolated from the rhizosphere of sand dune plants were examined using a polyphasic approach. The analysis of the 16S rRNA gene sequence indicated that all of the isolates fell into four distinct phylogenetic clusters belonging to the genus Chryseobacterium of the family Flavobacteriaceae. The 16S rRNA gene sequence similarities of isolates to mostly related type strains of Chryseobacterium ranged from 97.5% to 98.5%. All strains contained MK-6 as the predominant menaquinone, and iso-C 15:0, iso-C 17:0 3-OH and a summed feature of iso-C 15:0 2-OH and/or C 16:1 ω7c as the dominant fatty acids. Combined phenotypic, genotypic and chemotaxonomic data supported that they represented four novel species in the genus Chryseobacterium, for which the names Chryseobacterium hagamense sp. nov. (type strain RHA2-9 T=KCTC 22545 T=NBRC 105253 T), Chryseobacterium elymi sp. nov. (type strain RHA3-1 T=KCTC 22547 T=NBRC 105251 T), Chryseobacterium lathyri sp. nov. (type strain RBA2-6 T=KCTC 22544 T=NBRC 105250 T), and Chryseobacterium rhizosphaerae sp. nov. (type strain RSB3-1 T=KCTC 22548 T=NBRC 105248 T) are proposed. 相似文献
17.
A Gram-staining-negative, non-motile, catalase- and oxidasepositive bacterium, designated strain I-15 T, was isolated from a crab of the Yellow Sea, Korea. On the basis of a 16S rRNA gene sequence analysis, strain I-15 T was shown to belong to Bacteroidetes, related to the genus Aestuariibaculum. Sequence similarity between strain I-15 T and the only type strain of the genus Aestuariibaculum, Aestuariibaculum suncheonense SC17 T, was 96.7%. Strain I-15 T grew at 0.5–6.0% (w/v) NaCl, at 10–42°C and at pH 4.5–8.0. It could hydrolyze starch and Tweens 80. Menaquinone-6 was the only respiratory quinone, and summed features 3 (C 16:1 ω7 c/C 16:1 ω6 c) (16.4%), iso-C 15:0 (15.6%), and iso-C 15:1 G (12.6%) were the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content was 39.0 mol%. Polyphasic data allowed genotypic and phenotypic distinction of strain I-15 T from the only validly published Aestuariibaculum species. Therefore, the organism is considered a novel species of the genus Aestuariibaculum, for which the name Aestuariibaculum scopimerae sp. nov. is proposed. The type strain is I-15 T (=KCTC 32459 T =JCM 19486 T). 相似文献
18.
A novel Gram-negative, orange pigmented, strictly aerobic bacterium, designated strain IP9 T, was isolated from seawater at the sea shore of Incheon Eulwang-ri beach, South Korea. Cells of strain IP9 T were observed to be straight or slightly curved rods and colonies to be round and convex. Strain IP9 T was found to be catalase and oxidase positive, and non-motile. Growth was observed in the temperature range of 10–37 °C (optimum at 30 °C), pH range of 6–10 (optimum at pH 7–8) and salt concentration range of 0–7% (w/v) NaCl (optimum at 0–1%). On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, strain IP9 T was found to be related to the members of the family Flavobacteriaceae, being closely related to Hwangdonia seohaensis KCTC 32177 T (95.3% sequence similarity). The DNA G?+?C content of the novel strain was determined to be 39.1 mol%. The major polar lipids were found to be phosphatidylethanolamine, three unidentified aminoglycolipids and two unidentified glycolipids. The major fatty acids (>?10%) were identified as iso-C 15:0 and iso-C 17:0 3-OH. The predominant quinone was found to be menaquinone 6 (MK-6). Based on the biochemical, phylogenetic and physiological data, we conclude that strain IP9 T (=?KCTC 52523 T?=?JCM 31732 T) represents the type species of a novel genus of the family Flavobacteriaceae for which the name Thalassorhabdus aurantiaca gen. nov., sp. nov. is proposed. 相似文献
19.
A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5 T, was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5 T belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979 T (98.4 %) and Deinococcus daejeonensis KCTC 13751 T (97.5 %). Strain N5 T exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D 10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C 16:1 ω7c (25.25 %), C 15:1 ω6c (19.77 %), C 17:1 ω6c (11.87 %), and C 17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5 T as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. nov. is proposed. The type strain is N5 T (=KCTC 33153 T = JCM 19176 T). 相似文献
20.
Strain S22 T, a novel cellulolytic bacterium was isolated from the rhizosphere of pine trees. This isolate was Gram-reaction positive,
motile and rods, and formed terminal or subterminal ellipsoidal spores. S22 T represented positive activity for catalase, oxidase, esterase (C4), esterase lipase (C8), β-galactosidase, leucine arylamidase,
and hydrolysis of esculin. It contained meso-diaminopimelic acid as the diagnostic dia-mino acid in the cell-wall. The predominant
isoprenoid quinone was menaquinone 7 (MK-7), and the major cellular fatty acids were anteiso-C 15:0 (52.9%), iso-Ci 16:0 (11.3%), and iso-C 15:0 (10.0%). The DNA G+C content was 43.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that this isolate
belonged to the family Paenibacillaceae. S22 T exhibited less than 97.0% 16S rRNA gene similarity with all relative type strains in the genus Paenibacillus, and the most closely related strains were Paenibacillus anaericanus MH21 T and Paenibacillus ginsengisoli Gsoil 1638 T, with equal similarities of 95.8%. This polyphasic evidence suggested that strain S22 T should be considered a novel species in the genus Paenibacillus, for which the name, Paenibacillus pini sp. nov., is proposed. The type strain is S22 T (=KCTC 13694 T =KACC 14198 T =JCM 16418 T) 相似文献
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