头花马先蒿和管花马先蒿的化学成分

从头花马先蒿全草中发现13个化合物,经波谱分析鉴定为：（±）-松脂素（1）、金合欢素（2）、木犀草素（3）、7．去氧栀子新苷（4）、yuheinoside（5）、euphroside（6）、mussaenosidic acid（7）、莫桑苷（8）、桃叶珊瑚苷（9）、角胡麻苷（10）、异角胡麻苷（11）、贞桐苷A（12）和开德苷元（13）。从管花马先蒿全草中发现4个化合物,经鉴定为：（＋）．dehydrovomifoliol（14）、催吐萝芙木醇（15）、ω-hydroxypropioguaiacone（16）和3-hydroxy-1-（4-hydroxy-3,5-dimethoxyphenyl）-1-propanone（17）。所有化合物均为首次在该种中发现。     

栗柄金粉蕨的黄酮类成分

从栗柄金粉蕨（ Ｏｎｙｃｈｉｕｍ ｌｕｃｉｄｕｍ ） 地上部分的甲醇抽提物中分到１０ 个成分, 经详细的一维、二维核磁数据分析, 它们被鉴定为： 木犀草甙（１） , ３ , ７ － 二甲基槲皮素（２） , 高山甙Ｂ （３） , 金粉蕨素（４） , 栗柄醇（５） , 金粉蕨醇Ｂ（６） , β－ 谷甾醇（７） , 胡萝卜甙（８） ,齐墩果酸（９） 和蔗糖（１０） 。栗柄醇系新成分。１ , ２, ９ 系首次由金粉蕨属分到。     

Constituents with tyrosinase inhibitory activities from branches of Ficus erecta var. sieboldii King

Phytochemical investigation of the branches of Ficus erecta var. sieboldii King resulted in the isolation of eight constituents: p-hydroxybenzoic acid (1), methyl p-hydroxybenzoate (2), vanillic acid (3), methyl vanillate (4), syringic acid (5), β-sitosterol (6), α-amyrin acetate (7), and ethyl linoleate (8). Their chemical structures were identified via spectroscopic means as well as by comparing their data with literature values. Studies on tyrosinase inhibition activities were conducted for the isolated compounds. Among them, p-hydroxybenzoic acid (1) and methyl p-hydroxybenzoate (2) were identified as active tyrosinase inhibitors with IC(50) values of 0.98?±?0.042 and 0.66?±?0.025?mM, respectively, showing comparable activities to that of arbutin (IC(50)?=?0.32?±?0.015?mM), a standard control. Inhibition kinetics, as analyzed by Lineweaver-Burk plots, indicated that compounds 1 and 2 were competitive inhibitors of diphenolase of mushroom tyrosinase. Notably, isolated compounds 1-8 were reported for the first time as constituents of F. erecta.     

Acylated iridoid glucosides from Veronica anagallis-aquatica

Three new (1-3) and four known iridoid glucosides (4-7) as well as a known phenylethanoid glycoside (8) were isolated from the aerial parts of Veronica anagallis-aquatica and their structures were determined as 6'-O-benzoyl-8-epiloganic acid named aquaticoside A (1), 6'-O-p-hydroxybenzoyl-8-epiloganic acid named aquaticoside B (2), 6'-O-benzoyl-gardoside named aquaticoside C (3), veronicoside (4), catalposide (5), verproside (6), verminoside (7) and martynoside (8) on the basis of 1D and 2D NMR spectral analysis.     

Two non-identical heads of myosin molecules

Four different preparations of skeletal subfragment-1, denoted in this report as S1(Aa), S1(Ab), S1(Ba), and S1(Bb), and two different preparations of cardiac subfragment-1, denoted as S1(A) and S1(B), were obtained as described in our recent report (J. Biochem. 97, 965, 1985). (i) The four preparations were obtained from chicken breast myosin trinitrophenylated with 2,4,6-trinitrobenzene sulfonate in the absence of inorganic pyrophosphate (-PPi), and they were all shown to be trinitrophenylated. Addition of PPi caused change in the absorption spectra of trinitrophenyl(TNP)-S1(Aa) and TNP-S1(Ba), but not in those of TNP-S1(Ab) and TNP-S1(Bb). (ii) The two preparations of S1 were obtained from cardiac myosin trinitrophenylated either in the absence (-) or presence (+) of PPi. S1(B) was trinitrophenylated, whereas S1(A) was not. Specifically emphasized is the observation that the yield of cardiac S1(A) was practically equal to that of cardiac S1(B). On the basis of these results, we propose the hypothesis of "two iso-myosins with non-identical heads," which is essentially a combination of the hypothesis of isoenzymes and that of non-identical heads.     

Triterpene saponins from Randia formosa

Seven new triterpenoid saponins, randiasaponins I (1), II (2), III (3), IV (4), V (5), VI (6) and VII (7) as well as two known ones, ilexoside XXVII (8) and ilexoside XXXVII (9), were isolated from the methanolic extract of the leaves of Randia formosa. The structures of the new saponins were established as 3-O-alpha-L-arabinopyranosyl-3 beta,19 alpha,23-trihydroxyursa-12,20(30)-dien-28-oic acid 28-beta-D-glucopyranosyl ester (1), 3-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranosyl rotundic acid (2), 3-O-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranosyl pomolic acid 28-beta-D-glucopyranosyl ester (3), 3-O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl pomolic acid 28-beta-D-glucopyranosyl ester (4), 3-O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranosyl siaresinolic acid 28-beta-D-glucopyranosyl ester (5), 3-O-alpha-L-arabinopyranosyl ilexosapogenin A 28-beta-D-glucopyranosyl ester (6), and 3-O-beta-D-glucopyranosyl ilexosapogenin A 28-beta-D-glucopyranosyl ester (7), based on spectral and chemical evidence. Besides the saponins, two common flavonoids kaempferol 3-O-rutinoside and rutin were also isolated.     

荆三棱化学成分的研究（I）

我们前期从红花锦鸡儿(Caragana rosea)中发现两个具有抗HIV活性成分seirpusin A和B(含量仅约百万分之一),为了寻找富含该化合物的新的植物资源,我们对产于黑龙江省的荆三棱(Scirpus yagara)的化学成分进行了研究,从其乙醇提取物的乙酸乙酯萃取部位分离到6个化合物.通过1H NMR分析及与对照品TLC比较,分别鉴定为:白桦醇(1),对羟基桂皮酸(2),scirpusin A(3),白藜芦醇(4),piceatannol(5)及scirpusin B(6).其中,化合物scirpusin A和B,在该植物中含量丰富(含量分别为0.017%和0.013%).     

川芎内生菌Pseudeurotium ovale代谢产物研究

通过大孔树脂柱层析、凝胶柱层析和高效液相色谱等方法对川芎中分离得到的内生菌Pseudeurotium ovale的代谢物进行分离纯化,从中分离得到5个化合物,并通过核磁共振和质谱等波谱学技术手段确认所有化合物的结构,其中包含一个新的化合物4-methoxy-3-methyl-6-(1 E,3 E)-1,3-pentadien-1-yl-2 H-pyran-2-one(1)和四个首次从中分离得到的已知化合物:(2 E,4 E)-1-(2,6-dihydroxy-3,5-dimethylphenyl)-2,4-hexadien-1-one(2)、2-deoxy-sohirnone C(3)、trichodimerol(4)、3,7-dihydroxy-1,9-dimethyl-dibenzofuran(5)。体外抗炎活性测试表明化合物1可抑制经脂多糖(LPS)处理的RAW 264.7细胞中NO、IL-6和TNF-α等炎症因子的分泌。     

马尾伸筋草化学成分及抗骨质疏松活性研究

为研究马尾伸筋草(Lycopodium fargesii Hert.)的化学成分及其抗骨质疏松活性,采用硅胶、MCI、RP-18、Sephadex LH-20柱色谱及半制备高效液相色谱对马尾伸筋草的乙醇提取物进行系统分离,从乙酸乙酯部位中得到18个化合物,综合利用NMR、HR-ESI-MS等技术,分析鉴定化合物的结构,分别鉴定为α-芒柄蜡素(1)、26-去甲-8-氧化-α-芒柄蜡素(2)、serrat-14-en-3β,21α,24-triol(3)、serrat-14-en-3β,21β,24-triol(4)、1,20-eicosanediol(5)、二十三烷(6)、glycerolmonolinoleate(7)、glyceryl linolentate(8)、3-(4′-formylphenoxy)-4-methoxybenzaldehyde(9)、芹菜素(10)、对羟基苯甲醛(11)、香草酸(12)、3-羟基-4-甲氧基苯甲酸(13)、2-羟基-1-(4-羟基-3-甲氧基-苯基)-丙基-1-酮(14)、3-甲氧基-4-羟基桂皮醛(15)、8-acetoxy-5-hydroxyumbelliprenin(16)、trans-4-hydroxy-2-nonenoic acid(17)和正十烷硫醇(18)。化合物1~18均为首次从马尾伸筋草中分离得到,其中化合物16对破骨细胞活性具有显著的抑制作用,其IC₅₀为1.98μM。     

南海链霉菌SCSIO 1635中抗霉素类化合物的分离鉴定(英文)

我们从南海海底沉积环境分离了一株放线菌SCSIO1635,经16S rDNA的序列分析将该株菌鉴定为链霉菌属。我们从该菌的发酵液中分离得到了4个化合物,经质谱和核磁共振波谱解析,确定为抗霉素类化合物:异构体antimycin A1a和A1b(1)、deisovalerylblastmycin(2)、kitamycin A(3)和antimycin A9(4)。     

大红袍中单宁化学成分的研究

从大红袍中分离出5个单宁化合物,通过光谱分析确定其结构分别为:epicatechin(?),procyanidin B-1(?),procyanidin B-2(?),procyanidin B-5(4)和 procyanidin C-1(5).上述化合物均为首次从该植物中分离得到。     

Studies on the Chemical Constituents of the Leaves of WTBX]Pseudotaxus chienii (Cheng) Cheng

Fourteen compounds were isolated from the leaves of Pseudotaxus chienii (Cheng) Cheng which is uniquely indigenous to China, and their structures were identified mainly by spectrum analyses. Among them, 13 known compounds were determined as: 2-guaiacylpropane-1, 3-diol (1), vanillic acid (2), 3-methoxy-4-hydroxy cinnamic acid (3). 3. 5-dimethoxy-phenol (4), taxicatin (5), 5-oxymaltol (6), quercetin-3-rhamnoside (7), (±)-catechin (8), ecdysterone (9), β-sitosterol (10), D-glucose (11), (±)-10-nonacosanol (12) and octacosanoic acid (13). All these compounds butβ-sitosterol were isolated from this plant for the first time. Compounds 1 and 6 have never been reported in plant kingdom. Another compound was tentatively established as a new compound, named pseudotaxlactone (14).     

Potent inhibition of bacterial neuraminidase activity by pterocarpans isolated from the roots of Lespedeza bicolor

Bacterial neuraminidase has been highlighted as a key enzyme for pathogenic infection and sepsis. Six pterocarpans displaying significant levels of neuraminidase inhibitory activity were isolated from the root bark of Lespedeza bicolor. The isolated compounds were identified as three new pterocarpans (1-3) together with known compounds erythrabyssin II (4), lespebuergine G4 (5), and 1-methoxyerythrabyssin II (6). The new compounds were characterized as bicolosin A (1), bicolosin B (2), and bicolosin C (3). All compounds inhibited bacterial neuraminidase in a dose-dependent manner with significant inhibition (IC(50)=0.09-3.25 μM). All neuraminidase inhibitors screened were found to exhibit noncompetitive kinetics. The three most potent neuraminidase inhibitors (1, 3 and 6) feature a methoxy substitution on C-1.     

Strain-rate dependent material properties of the porcine and human kidney capsule

This study was performed to characterize the mechanical properties of the kidney capsular membrane at strain-rates associated with blunt abdominal trauma. Uniaxial quasi-static and dynamic tensile experiments were performed on fresh, unfrozen porcine and human renal capsules at deformation rates ranging from 0.0001 to 7 m/s (strain-rates of 0.005-250 s(-1)). Single stroke, dynamic tests were performed on samples of porcine renal capsule at strain-rates of 0.005 s(-1) (n = 33), 0.05 s(-1) (n = 17), 0.5 s(-1) (n = 38), 2 s(-1) (n = 10), 4 s(-1) (n = 10), 50 s(-1) (n = 21), 100 s(-1) (n = 18), 150 s(-1) (n = 17), 200 s(-1) (n = 10), and 250 s(-1) (n = 17). Due to limited availability of human tissues, only quasi-static tests were performed (0.005 s(-1), n = 25). Porcine renal capsule properties were found to match the material properties of human capsular tissue sufficiently well such that porcine tissue material can be used as a human test surrogate. The apparent elastic modulus and breaking stress of the porcine renal capsule were observed to increase significantly with increasing strain-rate (p < 0.01). Breaking strain was inversely related to strain-rate (p < 0.01). The effect of increasing strain-rate on material properties diminished appreciably at rates exceeding 150 s(-1). Empirically derived mathematical models of constitutive behavior were developed using a hyperelastic/viscoelastic Ogden formulation, as well as a Cowper-Symonds law material curve multiplication.     

Chemical synthesis of peptide fragments of the hormone-specific beta-subunit of human follicle-stimulating hormone

In order to determine the specific antigenic determinants of human follicle-stimulating hormone (hFSH), hFSH-beta peptides with amino acid residues 33-49 (V2), 95-118 (V3), 76-118 (V3 + 1/2 C2), 1-33 (V1 + C1), 22-33 (1/2C1), and 95-107 (V3 + 1/4C2) according to the nomenclature of Stewart and Stewart [Stewart, M., & Stewart, F. (1977) J. Mol. Biol. 116, 175] as well as additional peptides with the residues 93-107, 91-107, 89-107, 87-107, and 85-107 were chemically synthesized. The peptides were examined in radioimmunoassay systems of FSH, luteinizing hormone (LH), or human chorionic gonadotropin (hCG). V3 + 1/2C2 and V1 + C1 showed immunological activity, whereas the other peptides did not. Antibodies were raised in rabbits against these peptides and examined for specific binding with hFSH, LH, thyroid-stimulating hormone (TSH), and hCG. V3 + 1/2C2 as well as V1 + C1 produced antisera, which specifically bound hFSH, hLH, and hTSH, indicating that the amino acid sequences contained in hFSH-beta peptides V3 + 1/2C2 and V1 + C1 share common antigenic sites with hLH and hTSH. Antisera were produced in rabbits against hFSH-beta, against reduced and S-aminoethylated hFSH-beta (AE-FSH-beta), and against AE-FSH-beta coupled to hemocyanin. Reduced and S-aminoethylated beta-subunit of FSH-beta coupled with hemocyanin produced antisera in rabbits that specifically bound only hFSH and not hLH, hTSH, or hCG.     

桂枝化学成分研究北大核心CSCD

桂枝是临床常用中药,本课题组前期发现桂枝乙醇提取物具有抑制程序性细胞坏死的生理活性。为进一步阐明桂枝的化学成分和更好地开发利用桂枝药用资源,该文采用大孔吸附树脂、硅胶柱色谱、Sephadex LH-20柱层析、制备型高效液相色谱等多种方法对桂枝75%乙醇提取物进行了研究。此次报道从中得到的13个单体化合物,它们的结构经波谱数据分析及文献对照鉴定为脱落酸(1)、蚱蜢酮(2)、2,3-二羟基-1-(4-羟基-3,5-二甲氧基苯基)-1-丙酮(3)、赤型-1,2,3-三羟基苯丙烷(4)、1-苯基-1,3-丙二醇(5)、香豆素(6)、肉桂酸(7)、对羟基肉桂酸(8)、邻羟基肉桂酸(9)、邻甲氧基肉桂酸(10)、肉桂醛(11)、阿魏酸(12)、咖啡酸乙酯(13)。其中1-5、12和13为首次从桂枝中分离得到。     

Triterpene saponins from Cylicodiscus gabunensis.

Four new saponins were isolated from the alcoholic extract of the bark of Cylicodiscus gabunensis by means of flash chromatography. They were characterized on the basis of spectral and chemical data as 3-O-beta-[alpha-L-arabinopyranosyl (1----2),alpha-L-arabinopyranosyl(1----3),beta-D-glucopyranosyl(1- ---)] maslinic acid-28-[beta-D-glucopyranosyl(1----6),beta-D-glucopyranosyl(1----2),alp ha-L- rhamnopyranosyl(1----)] ester; 3-O-beta-[alpha-L-arabinopyranosyl(1----2),alpha-L-arabinopyran osyl (1----3),beta-D-glucopyranosyl(1----)]maslinic acid-28-[beta-D-glucopyranosyl(1----2),alpha-L-rhamnopyranosyl(1----)] ester, 3-O-beta-[alpha-L-arabinopyranosyl(1----2),alpha-L-arabinopyran osyl (1----3),beta-D-glucopyranosyl(1----)]maslinic acid and 3-O-beta(alpha-L-arabinopyranosyl(1----3),beta-D-glucopyranosyl (1----)] cylicodiscic acid.     

棒柄花的化学成分研究

从棒柄花(Cleidion brevipetiolatum)的乙醇提取物中分离得到6个化合物,经波谱分析确定其结构为：benzyl-1-O-β-D-glumpyranoside(1),chavicol-1-O-β-D-glumpyranoside(2),3,4,5-trimethoxyphenol(3),3,4,5-trimethoxyphenol-1-O-β-D-glucopyranoside(4),β-sitosterol(5),daumsterol(6),以上化合物均为首次从该植物中分离得到。     

New Withanolides from Nicandra physaloides (Solanaceae)

Two new withanolides, named nicandrenone methyl ether (1), 26S nicandrenone methyl ether (2), together with ten known compounds were isolated from the whole plants of Nicandra physaloides (Solanaceae). Their chemical structures were deduced on the basis of spectroscopic analysis. Ten known compounds were identified as nicandrenone (3), Nic-7 (4), nicaphysalin E (5), pinosylvin monomethyl ether (6), 2S-pinocembrin (7), (1S, 2R)-1, 2-bis (4-hydroxy-3-methoxyphenyl)-1, 3-propanediol (8), vanillin (9), indole-3-carboxylic acid (10), vanillic acid (11) and drummondol (12).     

Secondary metabolites from the roots of Engelhardia roxburghiana and their antitubercular activities

Engelharquinone (1), engelharquinone epoxide (2), engelharolide (3), and engelhardic acid (4), were isolated as naturally occurring products from a plant source, Engelhardia roxburghiana together with 20 previously known compounds, four of which were hitherto not known as plant constituents. Their structures were identified by means of spectroscopic analysis. A biological evaluation showed that three of the previously isolated antitubercular constituents [(-)-4-hydroxy-1-tetralone, 3-methoxyjuglone and engelhardione] and engelharquinone (1) exhibited moderate antitubercular activity against Mycobacterium tuberculosis 90-221387.