共查询到20条相似文献,搜索用时 78 毫秒
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从基因组学到蛋白质组学 总被引:12,自引:0,他引:12
介绍了蛋白质的概念,产生背景,发展过程;比较了蛋白质组学与基因组学研究之间的差异;对结构蛋白质组学和功能蛋白质组学研究的内容以及相应的主要研究手段与技术加以简单评述,阐述了蛋白质组学研究的理论意义,应用前景及对国计民生的影响。 相似文献
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人类基因组计划的机遇和挑战:Ⅰ.从结构基因组学到功能基因组学 总被引:23,自引:0,他引:23
人类基因组计划的机遇和挑战:Ⅰ.从结构基因组学到功能基因组学陈竺*李伟俞曼熊慧陈赛娟(上海第二医科大学附属瑞金医院、血液学研究所、卫生部暨上海市人类基因组研究重点实验室*上海人类基因组研究中心、上海生命科学研究中心)关键词人类基因组计划结构基因组学功... 相似文献
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功能基因组学的研究内容与方法 总被引:37,自引:0,他引:37
基因组学的研究已从结构基因组学转向功能基因组学.综述了功能基因组学研究的内容和方法,主要包括应用微点阵、基因表达系列分析(SAGE)、蛋白质组、生物信息学等方法来研究基因组表达概况、基因组多样性、模式生物体等. 相似文献
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基因组学在基因组计划中的作用 总被引:4,自引:0,他引:4
导论科研人员正在为确定基因组研究的应用途径作着巨大努力。人类基因组计划(HGP)的诞生导致了对人类基因结构和功能的理解[1]。在20世纪末期,生命科学的主要焦点就是要解译和确定人类整个基因组的成分。对构成细胞活动基础的基因组DNA的理解,将有助于对各... 相似文献
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功能基因组学研究概述 总被引:2,自引:0,他引:2
21世纪是生物时代和信息时代,基因组学的研究已从结构基因组学转向功能基因组学,功能基因组学时代对于基因功能的研究也由单一基因转向大规模、批量分析。对功能基因组学及相关学科的概念作了概述,综述了功能基因组学的研究内容与方法,主要包括应用差异显示反转录PCR、基因表达序列分析(SAGE)、微点阵、蛋白质组学和生物信息学等方法来研究基因组表达概况、基因组多样性和模式生物学等。 相似文献
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水稻功能基因组学研究 总被引:4,自引:0,他引:4
水稻是迄今为止第一个被测序的农作物。随着水稻基因组测序计划的完成,以功能基因组学研究为标志的后基因组时代已经到来。综述了水稻功能基因组学的工作进展与方法,主要包括:表达序列标签(EST)c、DNA微阵列和DNA芯片、蛋白质组学、生物信息学和反向遗传学等新方法。 相似文献
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蛋白质组与蛋白质组学 总被引:4,自引:0,他引:4
王大东 《国外医学:分子生物学分册》2000,22(3):129-133
1994年澳大利有针对后基因组时代研究趋势,提出了蛋白质组和蛋白质组学的新概念。即把基因组所编码的所有蛋白为研究对象,直接探讨基因、蛋白的功能。其核心技术包括双向电泳、质谱分析、生物信息学等。该技术在探索疾病发生,寻找新药等方面取得越来越广泛的应用。 相似文献
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表面等离子体共振(surface plasmon resonance,SPR)依据光学—介质相互作用原理建立,属于实时和非标记的测试方法。SPR方法在研究分子间相互作用方面具有其独特的优势,其非标记和实时检测以及可以进行动力学分析的特点,给研究生物大分子的相互作用提供了诱人的解决方案。近来,随着SPR成像技术和SPR芯片制备技术的进展,将为功能基因组学和蛋白质组学研究提供重要的新的技术平台。 相似文献
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From genomics to proteomics 总被引:1,自引:0,他引:1
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化学基因组学和化学蛋白质组学作为后基因组时代的新技术,是以化学小分子为工具,对细胞的生理过程进行精确干扰,研究有机体和细胞的功能,同时也是新药开发的重要手段。本文综述了化学基因组学和化学蛋白质组学征自噬相关靶点的特异性小分子的发现,及小分子存自噬机理研究中的应用。 相似文献
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Jonathan Astin Alyce Merry Jeena Rajan Patricia E Kuwabara 《Briefings in Functional Genomics and Prot》2004,3(1):26-34
The nematode Caenorhabditis elegans is widely used as a model organism for studying many fundamental aspects of development and cell biology, including processes underlying human disease. The genome of C. elegans encodes over 19,000 protein-coding genes and hundreds of non-coding RNAs. The availability of whole genome sequence has facilitated the development of high throughput techniques for elucidating the function of individual genes and gene products. Furthermore, attempts can now be made to integrate these substantial functional genomics data collections and to understand at a global level how the flow of genomic information that is at the core of the central dogma leads to the development of a multicellular organism. 相似文献
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小麦的比较基因组学和功能基因组学 总被引:12,自引:1,他引:11
小麦是异源多倍体植物,具有大的染色体组,并且基因组中重复序列所占比例较高,这些特征限制了小麦基因组研究的进展。比较基因组学方法为运用模式植物进行小麦基因组学研究提供了一个操作平台。功能基因组学的研究集中于基因组中转录表达的部分,基因功能的确定是功能基因组学研究的主要内容。对比较基因组学在小麦基因组研究中的应用和小麦功能基因组学的研究内容和方法进行了综述。 相似文献
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Wiemann S Bechtel S Bannasch D Pepperkok R Poustka A;German cDNA Network 《Journal of structural and functional genomics》2003,4(2-3):87-96
Among the greatest challenges facing biology today is the exploitation of huge amounts of genomic data, and their conversion into functional information about the proteins encoded. For example, the large-scale cDNA sequencing project of the German cDNA Consortium is providing vast numbers of open reading frames (ORFs) encoding novel proteins of completely unknown function. As a first step towards their characterization we have tagged over 500 of these with the green fluorescent protein (GFP), and examined the subcellular localizations of these fusion proteins in living cells. These data have allowed us to classify the proteins into subcellular groups which determines the next step towards a detailed functional characterization. To make further use of these GFP-tagged constructs, a series of functional assays have been designed and implemented to assess the effect of these novel proteins on processes such as cell growth, cell death, and protein transport.Functional assays with such a large set of molecules is only possible by automation. Therefore, we have developed, and adapted, functional assays for use by robotic liquid handling stations and reading stations. A transport assay allows to identify proteins which localize to distinct organelles of the secretory pathway and have the potential to be new regulators in protein transport, a proliferation assay helps identifying proteins that stimulate or repress mitosis. Further assays to monitor the effects of the proteins in apoptosis and signal transduction pathways are in progress. Integrating the functional information that is generated in the assays with data from expression profiling and further functional genomics and proteomics approaches, will ultimately allow us to identify functional networks of proteins in a morphological context, and will greatly contribute to our understanding of cell function. 相似文献
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Zolnai Z Lee PT Li J Chapman MR Newman CS Phillips GN Rayment I Ulrich EL Volkman BF Markley JL 《Journal of structural and functional genomics》2003,4(1):11-23
A computing infrastructure (Sesame) has been designed to manage and link individual steps in complex projects. Sesame is being developed to support a large-scale structural proteomics pilot project. When complete, the system is expected to manage all steps from target selection to data-bank deposition and report writing. We report here on the design criteria of the Sesame system and on results demonstrating successful achievement of the basic goals of its architecture. The Sesame software package, which follows the client/server paradigm, consists of a framework, which supports secure interactions among the three tiers of the system (the client, server, and database tiers), and application modules that carry out specific tasks. The framework utilizes industry standards. The client tier is written in Java2 and can be accessed anywhere through the Internet. All the development on the server tier is also carried out in Java2 so as to accommodate a wide variety of computer platforms. The database tier employs a commercial database management system. Each Sesame application module consists of a simple user interface in the client tier, corresponding objects in the server tier, and relevant data stored in the centralized database. For security, access to stored data is controlled by access privileges. The system facilitates both local and remote collaborations. Because users interact with the system using Java Web Start or through a web browser, access is limited only by the availability of an Internet connection. We describe several Sesame modules that have been developed to the point where they are being utilized routinely to support steps involved in structural and functional proteomics. This software is available to parties interested in using it and assisting to guide its further development.Deceased, 30 August 2000 相似文献
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Takeshi Ishihara Kiyoshi Kakiya Koji Takahashi Hiroto Miwa Masatomo Rokushima Tomoyo Yoshinaga Yoshikazu Tanaka Takaomi Ito Hiroko Togame Hiroshi Takemoto Maho Amano Norimasa Iwasaki Akio Minami Shin-Ichiro Nishimura 《Biochimica et Biophysica Acta (BBA)/General Subjects》2014