Laboratory Hints from the Literature

Chaze, J. Sur le mode de formation et la detection des alcaloides dans la plantule de tabac. Bull. d'Histol. Appl., 5, 253. 1928.

Nan, Yao. Sur un fixateur cytologique particulièrement adapté aux tissues des insectes. Bull. d'Histol. Appl., 4, 71, 1927.

Reichardt, H., and Wetzel, A. Paraffineinbettungsmethode nach vorhergegangener Zelloidindurchtränkung unter Vermeidung der härtenden Intermedien, Xylol, Benzol, Chloroform. Zeit. f. wiss. Mikr., 45, 476-479. 1928.

Erb, N. M. A rapid stain for direct miroscopic examination of milk. J. Lab. & Clin. Med., 14, 377. 1929.

Galesesco, P., and Bratiano, S. Coloration des graisses par l'extrait alcoolique de Daucus carota. Compt. Rend. Soc. Biol., 99, 1460. 1928.

Goldner, J' Résultats obtenus chez la seiche par l'emploi de divers colorants vitaux Comp. Rend. Soc. Biol., 99, 1323. 1928.

Hadjioloff, A. Une modification rapide de la méthode de Weigert-Pal. Bull. d'Histol. Appl., 5, 431-434. 1928.

Hausdorf, G. Farbung zur Darstellung reifer Samenzellen im Hodenschnittpraparat. Zeit. f. wiss. Mikr., 44, 327-328. 1927.

Houcke, E. Emploi des colorants mixtes en technique histologique. Comp. Rend. Soc. Biol., 99, 783. 1928.

Houcke, E. Emploi des mélanges de fuchsines et de blues basiques pour la coloration histologique. Comp. Rend. Soc. Biol., 99, 786. 1928.

Houcke, E. Emploi du mélange rhodamine-blue de méthylène dans la coloration des tissus splénique et lymphcïde. Comp. Rend. Soc. Biol., 99, 788. 1928.

Lillie, R. D. Erne Schnellmethode zur Toluidinblau-Schleimfarbung. Zeit. f. wiss. Mikr., 45, 381. 1928.

Ochs, Georg Wilhelm. Über den Einfluss der Temperatur auf die Färbung von Blutausstrich-Präparaten. Folia Hematologica, 37, 241-257. 1928.

Verne, Jean. Une nouvelle coloration élective de la myeline. Bull. d'Histol. Appl., 5, 223-224. 1928.     

Contribuzione alla conoscenza della flora dell'Africa Orientale I Centuria

Summary

Determination of one century of East Africa's plants, collected by several students (Milchersich R., Romagnoli M., Cappelletti F., Nastasi V., Candusio R., Chiuderi A. Saccardo D., Capuano D., Jannone G. e Previeri O.) in Eritrea, Abyssinia and Italian Somalia during the yars from 1937 to 1951 and preserved in Erbario Coloniale at Florence.     

Microscopic Slides of Cat Testis

DYES AND THEIR BIOLOGICAL USES Copley, A. L., and Whitney, D. V. The standardization and assay of heparin by the toluidine blue and azure A reactions. A correction. J. Lab. &; Clin. Med., 29, 117.

Finkelstein, Jacob. N-substituted sulfonamides. J. Amer. Chem. Soc., 66, 407. 1944.

Fraenkel-Conrat, H., and Cooper, M. The use of dyes for the determination of acid and basic groups in proteins. J. Biol. Chem., 154, 239. 1941.

Gilman, Henry, and Shirley, David A. Some derivatives of pheno-thiazine. J. Amer Chem. Soc., 66, 888. 1944.

Gilman, Henry, and Spatz, Sydney M. Some quinolines patterned as “open models” of atabrine. J. Amer. Chem. Soc., 66, 621. 1944.

Klotz, Irving M. The mode of action of sulfonamides. J. Amer. Chem. Soc., 66, 459. 1944.

Mueller, Albert C. and Hamilton, Cliff S. Some derivatives of 7-methoxy- and 10-methoxybenzoquinoline. J. Amer. Chem. Soc., 66, 860. 1944.

Peterson, Osler L. Therapeutic effects of forbisen and of toluidine blue on experimental typhus. Proc. Soc. Exp. Biol. &; Med., 55, 155-7. 1944.

ANIMAL MICROTECHNIC Ballantyne, E. N. A staining method for frozen sections. Canad. J. Med. Techn., 2, 65-7. 1940.

Bodian, David, and Mellors, Robert C. Phosphatase activity in chromatolytic nerve cells. Proc. Soc. Exp. Biol. &; Med., 55, 248-5. 1844.

Forbes, J. Glycerine jelly mounting medium for frog eggs and early embryos. Trans. Amer. Micr. Soc., 62, 325-6. 1943.

Hughes, R. F. Laboratory hints. Canad. J. Med. Techn., 3, 25-6. 1940.

Krajian, Aram A. Elastic fibre stains. Canad. J. Med. Techn., 3, 207. 1941.

Kupperman, H. S., and Noback, C. R. A rapid iron hematoxylin tissue stain for laboratory use. Science, 98, 591-2. 1948.

Laws, S. G. A method of staining blood films. Canad. J. Med. Techn., 3, 68. 1941.

Lillie, R. D. Studies on the decalcification of bone. Amer. J. Path., 20, 291-6. 1944.

Moore, Margaret E. Twenty-hour schedule for routine tissue sections. Canad. J. Med. Techn., 2, 70-1. 1940.

Paul, Pauline, Lowe, B., and McClurg, B. R. Changes in histological structure and palatability of beef during storage. Food, Research, 9, 221-33.

Pinkus, Hermann. Acid orcein Giemsa stain (modification of Unna-Taenzer method). A useful routine stain for dermatologic sections. Arch. Dermat. &; Sypk., 49, 355-6. 1944.

Pugsley, Marion L. Reticulocyte staining. Canad. J. Med. Techn., 3, 16-7. 1940.

Slavkin, Alice E. Quick paraffin method for small biopsies. J. Lab. and Clin. Med., 29, 74. 1944.

PLANT MICROTECHNIC Stuart, Neil W., and Emsweller, S. L. Use of enzymes to improve cytological techniques. Science, 98, 569-70. 1943.

Wittlake, Eugene B. Permanent prestaining in botanical microtechnic. Ohio J. of Sci., 44, 36-8. 1944.

MICROÖRGANISMS Alexander-Jackson, Eleanor. A differential triple stain for demonstrating and studying non-acid-fast forms of the tubercle bacillus in sputum, tissue and body fluids. Science, 99, 307-8. 1944.

Barritt, M. M. An improved Pappenheim stain for gonococci. Brit. Med. J., 494, 4344. 1944.

Bartholomew, J. W., and Umbreit, W. W. Ribonucleic acid and the Gram stain. J. Bad., 47, 415. 1944.

Bauer, William H. Tooth buds and jaws in patients with congenital syphilis. Amer. J. Path., 20, 897-319. 1944.

Begg, A. M., Fulton, F., and Van Den Ende, M. Inclusion bodies in association with typhus rickettsiae. J. Path. &; Bact., 56, 109-13. 1944.

Cherewick, W.J. Studies on the biology of Erysiphe graminis DC. Canad. J. Research, 22, 58-85. 1944.

Dissmann, Edwin. Erfahrungen mit der karbolnachtblaufärbung der Tuberkelbazillen nach Hallberg. Zentbl. Bald., I Abt. Orig., 150, 268-75. 1943.

Hunt, George A. A study of the Pappenheim stain. A stable modification. J. Lab. &; Clin. Med., 20, 207-10. 1944.

Kirsh, David, and Schenken, John R. A comparison of the Ziehl-Neelsen and the Moss cold carbol fuchsia stains for acid-fast bacilli. New Orleans Med. and Surg. J., 96, 394-6. 1944.

Lee, H. I. Comparison of procedures for staining tubercle bacilli in fluorescent microscopy. J. Lab. &; Clin. Med., 29, 218-21. 1944.

Noble, Glenn A. A five-minute method for staining fecal smears. Science, 100, 87-8. 1944.

HISTOCHEMISTRY Dische, Zachakias. Two characteristic and sensitive color reactions between sulfhydryl compounds and thymonucleic acid. Proc. Soc. Exp. Biol. &; Med., 55, 217-8. 1944.

Wilmer, Harry A. Failure to demonstrate alkaline phosphatase activity in inclusion bodies by the bistochemical technic. Proc. Soc. Exp. Biol. &; Med., 55,206-7. 1944.     

Book reviews

The Art of Primitive Peoples. J. T. Hooper and C. A. Burland. (168 pp., 68 pls. The Fountain Press, 46–47 Chancery Lane, London W.C.2. 1953. Cloth bound 42/—).

The God of the Witches. Margaret A. Murray. (2nd ed. Faber & Faber, London 1952).

Magic Books from Mexico. C. A. Burland. (31 pp., 16 colour pls. King Penguin Books, Harmondsworth, Middlesex, England 1953. Bound 4/6 d).

Outline of South American Cultures. George P. Murdock. (Behavior Science Outlines, Vol. II, Human Relations Files, New Haven 1951. Price: $ 2.50).

Kunst im Reiche der Inca. Heinrich Ubbelohde Doering. (66 pp., 240 pls., 4 colour pls., one map. Verlag Ernst Wasmuth, Tübingen 1952. Cloth bound DM. 42).

Tiahuanacu, Atacama und Araukaner. Walter Ruben. (262 pp., 70 ill., 3 maps. Otto Harrassowitz Verlag, Leipzig 1952. Paper wrapper DM 15.80, bound DM 17.20).

Kunapipi. Ronald M. Berndt. (223 pp. Melbourne 1951), and Djanggawul. (230 pp. London 1952).     

Laboratory Hints from the Literature

Fry, H. J. A paraffin method for serially sectioning a minute object in a known plane. Anat. Rec., 34, 245-252. 1927.

Kisser, J. and Anderson, B. B. Method of preparing thin cross and longitudinal sections of cotton fibers and its importance in cell wall research. Am. J. Bot., 15, 437. 1928.

Sweany, H. C. System of sputum analysis for the presence of acid-fast bacilli. J. Lab. & Clin. Med., 14, 547-557. 1929.

Terry, B. T. Improvement in technic and results made in examining microscopically by the razor section method 2000 malignant tissues. J. Lab. & Clin. Med., 14, 519. 1929.

Belling, J. A method for the study of chromosomes in pollen-mother-cells. Univ. of Cal. Publs, in Bot., 14, 293-299. 1928.

Cartwright, K. St. G. A satisfactory method of staining fungal mycelium in wood sections. Ann. Bot., 43, 412. 1929.

Dolfini, G. Su un nuovo metodo di colorazione dei grassi. Bull. d'Histol. Appl., 6, 137. 1929.

Doubrow, S. Un procédé rapide de coloration des bacilles de Koch dans les coupes histologiques. Bull. d'Histol. Appl., 6, 142. 1929.

Hadjioloff, A. Coloration des graisses par quelques pigments naturels. Bull. d'Histol. Appl., 5, 183. 1929.

Joyet-Lavergne, Ph. La recherche qualitative du glutathion. Bull. d'Histol. Prac., 5, 331-349.

Levine, M. A method for staining connective tissue mast cells. J. Lab. & Clin. Med., 14, 172. 1928.

Martens, P. Les structures nucléaires et chromosomiques dans la cellule vivante et dans la cellule fixée. Bull. d'Histol. Appl., 5, 229-252. 1928.

Parker, F. P. A technic for staining red blood cells for measurement of cell diameters by the projection method. J. Lab. & Clin. Med., 14, 663. 1929.

Parker, F. P. and Lewis, G. T. Microscopic projections in the measurements of erythrocytes. J. Lab. & Clin. Med., 14, 664. 1929.

Pergola, M. Substrati al tellurito e colorasione ridotta nella ricerca e dimostrazione del bacillo difterico. Bull, dell' 1st. Sieroterapico Milanese, 7, 585-598. 1928.

Volkonsky, M. Sur une nouvelle modification de la technique d'Altman. Bull. d'Histol. Appl., 5, 220-222. 1928.

Webber, J. M. Smear method for the study of chromosomes. Univ. of Cal. Publs. in Bot., 14, 345-352. 1929.     

Expression of multiple Src family kinases in sea urchin eggs and their function in Ca release at fertilization

Egg activation at fertilization in deuterostomes requires a rise in intracellular Ca²⁺, which is released from the egg's endoplasmic reticulum. In sea urchins, a Src Family Kinase (SpSFK1) is necessary for the PLCγ-mediated signaling event that initiates this Ca²⁺ release (Giusti, A.F., O'Neill, F.J., Yamasu, K., Foltz, K.R. and Jaffe, L.A., 2003. Function of a sea urchin egg Src family kinase in initiating Ca2+ release at fertilization. Dev. Biol. 256, 367-378.). Annotation of the Strongylocentrotus purpuratus genome sequence led to the identification of additional, predicted SFKs (Bradham, C.A., Foltz, D.R., Beane, W.S., Amone, M.I., Rizzo, F., Coffman, J.A., Mushegian, A., Goel, M., Morales, J., Geneviere, A.M., Lapraz, F., Robertson, A.J., Kelkar, H., Loza-Coll, M., Townley, I.K., Raisch, M., Roux, M.M., Lepage, T., Gache, C., McClay, D.R., Manning, G., 2006. The sea urchin kinome: a first look. Dev. Biol. 300, 180-193.; Roux, M.M., Townley, I.K., Raisch, M., Reade, A., Bradham, C., Humphreys, G., Gunaratne, H.J., Killian, C.E., Moy, G., Su, Y.H., Ettensohn, C.A., Wilt, F., Vacquier, V.D., Burke, R.D., Wessel, G. and Foltz, K.R., 2006. A functional genomic and proteomic perspective of sea urchin calcium signaling and egg activation. Dev. Biol. 300, 416-433.). Here, we describe the cloning and characterization of these 4 additional SFKs and test their function during the initial Ca²⁺ release at fertilization using the dominant-interfering microinjection method coupled with Ca²⁺ recording. While two of the new SFKs (SpFrk and SpSFK3) are necessary for Ca²⁺ release, SpSFK5 appears dispensable for early egg to embryo transition events. Interestingly, SpSFK7 may be involved in preventing precocious release of Ca²⁺. Binding studies indicate that only SpSFK1 is capable of direct interaction with PLCγ. Immunolocalization studies suggest that one or more SpSFK and PLCγ are localized to the egg cortex and at the site of sperm-egg interaction. Collectively, these data indicate that more than one SFK is involved in the Ca²⁺ release pathway at fertilization.     

Analysis of the high- and low-spin Soret bands of horse-heart metmyoglobin complexes

Interest in the thermodynamics of the iron-binding site in hemoproteins has increased in recent years due to refinements in x-ray crystallographic studies of hemoproteins [see Deathage, J. F., Lee, R. S., Anderson, C. M. & Moffat, K. (1976) J. Mol. Biol. 104 , 687–706; Heidner, E. J., Ladner, R. C. & Perutz, M. F. (1976) J. Mol. Biol. 104 , 707–722; Deathage, J. F., Lee, R. S. & Moffat, K. (1976) J. Mol. Biol. 104 , 723–728; Ladner, R. C., Heidner, E. J. & Perutz, M. F. (1976) J. Mol. Biol. 114 , 385–414; Fermi, G. & Perutz, M. F. (1977) J. Mol. Biol. 114 , 421–431; Takano, T. (1977) J. Mol. Biol. 110 , 537–568 and 569–589], the synthesis and x-ray analysis of model heme compounds [see Scheidt, W. R. (1977) Acc. Chem. Res. 10 , 339–345; Kastner, M. E., Scheidt, W. R., Mashino, T. & Reed, C. A. (1978) J. Am. Chem. Soc. 100 , 666–667; Mashiko, T., Kastner, M. E., Spartalian, K., Scheidt, W. R. & Reed, C. A. (1978) J. Am. Chem. Soc. 100 , 6354–6362; Hill, H. A. O., Skite, P. P., Buchler, J. W., Luchr, H., Tonn, M., Gregson, A. K. & Pellizer, G. (1979) Chem. Commun. 4 , 151–152; and Scheidt, W. R., Cohen, I. A. & Kastner, M. E. (1979) Biochemistry 18 , 3546–3556], and the numerous data on heme–protein interactions that account for the differences observed in ligand binding between the various species of animals. Numerous probes have been used and provide information about the structure and thermodynamics of the binding site, but no single probe can provide the complete picture [see Iizuka, T. & Yonetani, T. (1970) Adv. Biophys. 1 , 157–182; Smith, D. W. & Williams, R. J. P. (1970) Struct. Bond. 7 , 1–45; and Spiro, T. G. (1975) Biochim. Biophys. Acta 416 , 169–189].     

Items from the Literature

Conn, H. J. Biological Stains. 7th ed., 355 pages, 27 figures, 9 × 6 inches, hard covers. The Williams &;: Wilkins Company, Baltimore 2, Maryland. $9.00. 1961.

Electron Microscopy Reimer, L., and GADACZ, H. Zur Schichtdickenkontrolle bei der elektronenmikroskopischen Schrägbeschattungsmethode. Z. wiss. Mikr., 65, 105-17. 1961.

Dyes and Their Biological Uses Macconaill, M. A., and GURR, E. The faviolic acid group of stains in histology. Irish J. Med. Sci., 1962, 1-12. 1962.

Animal Microtechnic Berres, H. H. Zur Darstellung peripherer Nervenfasem in der Haut. Z. wiss. Mikr., 65, 63-9. 1961.

Elias H., Henning A., and ELIAS P. M. Contributions to the geometry of sectioning. V. Some methods for the study of kidney structure. Z. wiss. Mikr., 65, 70-82. 1961.

Ewen, A. B. An improved aldehyde-fuchsin staining technique for neurosecretary products in insects. Tr. Amer. Micr. Soc, 81, 94-6. 1962.

Movat, Henry Z., STEINER, JAN W., and HUHN, DIETER. The fine structure of the glomerulus in acute glomerulonephritis. Lab. Investig., 11, 117-35. 1962.

Shaver, Evelyn L. The chromosomes of the opossum, Didelphis virginiana. Canad. J. Genet. Cytol. 4, 62-8. 1962.

Microorganisms Laird, Marshall. Trichodinids and other parasitic protozoa from the intertidal zone at Nanaimo, Vancouver Island. Canad. J. Zool., 39, 833-44. 1961.

Histochemistry Burstone, M. S. New histochemical techniques for the demonstration of tissue oxidase (cytochrome oxidase). I. Hisiochem. Cytochem., 7, 112-22. 1959.

Glenner, G. G., and LILLIE, R. D. Observations on the diazotization-coupling reaction for the histochemical demonstration of tyrosine: metal chelation and formazan variants. J. Histochem. Cytochem., 7, 416-22. 1959.

Hess, R., and Pearse, A. G. E. Histochemical demonstration of uridine diphosphate glucose dehydrogenase. Experientia, 15, 317-8. 1961.

LOVE, R., and LILES, R. H. Differentiation of nucleoproteins by inactivation of protein-bound amino groups and staining with toluidine blue and ammonium molybdate. J. Histochem. Cytochem., 7, 164-81. 1959.

Zimmerman, H., and Pearse, A. G. E. Limitations in the histochemical demonstration of pyridine nucleotide-linked dehydrogenases (“nothing dehydrogenase”). J. Histochem. Cytochem. 7, 271-5. 1959.     

PpAtg9 Trafficking During Micropexophagy in Pichia pastoris

PpAtg9 is essential for the selective degradation of peroxisomes (e.g., pexophagy) in Pichia pastoris. This integral membrane protein is synthesized in the endoplasmic reticulum (ER) and transported to a unique peripheral compartment (Atg9-PC). A putative ER exit motif has been identified and when deleted results in the accumulation of PpAtg9 within the ER. Upon the onset of micropexophagy, PpAtg9 transits from the Atg9-PC to perivacuolar structures (PVS) and sequestering membranes (SM) that arise from the vacuole to engulf the peroxisomes. In this article, we will discuss the transport pathways of PpAtg9 and those factors responsible for its trafficking.

Addenda to:

PpATG9 Encodes a Novel Membrane Protein that Traffics to Vacuolar Membranes which Sequester Peroxisomes during Pexophagy in Pichia pastoris

T. Chang, L.A. Schroder, J.M. Thomson, A.S. Klocman, A.J. Tomasini, P.E. Strømhaug and W.A. Dunn, Jr.

Mol Biol Cell 2005; 16:4941-53     

Ricerche Sul Fitoplancton del Mare Ligure

Riassunto

Viene completato l'elenco sistematico dei Ceratium viventi nelle acque di Sanremo, descrivendone una nuova specie: Ceratium Brunellii Rampi n. sp., e se ne stabilisce in linca di massima il comportamento fenologico.

Il raffronto del quadro feno-biologico dei Cerazi di Sanremo con quello di altre località mediterranee, sembra indicare che la distribuzione superficiale e la fenologia di questi microplanctonobi, è sensibilmente identica per tutto il bacino mediterraneo e che il gruppo afanotermo delle acque di Sanremo è ad apparizione precoce nei confronti di altre località mediterrance     

Book Review

A DEPARTMENT DEVOTED TO ABSTRACTS OF BOOKS AND PAPERS FROM OTHER JOURNALS DEALING WITH STAINS AND MICROSCOPIC TECHNIC IN GENERAL

RILEY, JAMES F. The Mast Cells. 182 pp., 65 figures. Size 81/2 by 10 inches. Price $6.75. E. and S. Livingstone Ltd., Edinburgh and London. 1959. Agent in the U. S. A., The Williams & Wilkins Co., Baltimore 2, Md.

MICROSCOPE AND OTHER APPARATUS GARIN, A., and THELLIER, M. Méthode de microdétermination et de microdosage de Bore dans une solution ou un tissu végétal par activation aux neutrons et examen microscopique des autoradiographies. Bull. d'Micr. Appl., Ser. 2, 8, 129-48. 1958.

KUHL, W., and FISCHER, H. Vertikal-, Horizontal-, und Umkehrmikroskop, in Verbindung mit einer Filmkamera, mit schnellem Funktionswechsel und geringem Gewicht. Zschr. wiss. Mikr. 64, 73-83. 1959.

MAI, G., and HEINE, U. Beschreibung einer Mikrofilmeinrichtung mit Zeitraffung. Zschr. wiss. Mikr. 64, 65-72. 1959.

TERTIAN, ROBERT, and TRILLAT, JEAN-JACQUES. Les applications du titane en microscopie électronique. Bull. d'Micr. Appl., Ser. 2, 8, 1-6. 1958.

MICROTECHNIC IN GENERAL HIRSCH, TH. v., and BOELLAARD, J. W. Methacrylsäureester als Einbettungsmittel in der Histologie. Zschr. wiss. Mikr. 64, 24-9. 1958.

PIEKARSKI, G. Über mikroskopisch erfassbare Rückstände in einigen sog. flüchtigen organischen Fixierungsmitteln. Zschr. wiss. Mikr. 63, 499-502. 1958.

WALTER, FRIEDRICH. Die Wirkungsweise der Fliessbewegung von Polymethacrylate bei der Herstellung von Ultradünnschnitten. Zschr. wiss. Mikr. 64, 106-10. 1959.

ARVY, LUCIE. Mise en évidence simultanée des lipides figués et des substances métachromatique. Bull. Micr. Appl., Ser. 2, 8, 120-124. 1958.

DE GROODT, M., LAGASSE, A., and SEBRUYNS, M. Vesikulationsvorgänge der Blut-Luftschranke der Lunge. Zschr. wiss. Mikr. 64, 90-4. 1959.

JUSTER, M. Contribution a l'étude du tissue osseux non dimeneralisé. Bull. Micr. Appl., Ser. 2, 9, 34-36. 1959.

PONLOT, ROBERT. L'intérět du noir Soudan B en histologie des os. Bull. Micr. Appl., Ser. 2, 8, 125-126. 1958.

WUNDERLICH, H. Blutzählkammern mit fluoreszierender Netzteilung für Fluoreszenzuntersuchungen. Zschr. wiss. Mikr. 64, 47-9. 1958.

PLANT MICROTECHNIC HEIMBURGER, M. Cytotaxonomic studies in the genus Anemone. Canad. J. Bot. 37, 587-612. 1959.

MORRISON, J. W. Cytogenetic studies in the genus Hordeum. I. Chromosome morphology. Canad. J. Bot. 37, 527-38. 1959.

MICROORGANISMS BORZANI, W., and VAIRO, M. L. R. Quantitative adsorption of crystal violet by dead microorganisms. J. Bact., 77, 757-9. 1959.     

Black leadership in the United States: Douglass,Garvey and King compared

Eric J. Sundquist (ed.), FREDERICK DOUGLASS: NEW LITERARY AND HISTORICAL ESSAYS, Cambridge: Cambridge University Press, 1990, 295 pp., £30.00.

Judith Stein THE WORLD OF MARCUS GARVEY: RACE AND CLASS IN MODERN SOCIETY, Baton Rouge, LA: Louisiana State University Press, 1986, 294 pp., £27.50.

Clayborne Carson, Ralph E. Luker, Penny A. Russell, (eds), THE PAPERS OF MARTIN LUTHER KING, JR., VOLUME I: CALLED TO SERVE, JANUARY 1929‐JUNE 1951, Berkeley, CA: University of California Press, 1992, 484 pp., $35.00.     

Updated Protocols and Comments on the Purification without Use of Activating Ligands of the Coupling Proteins Ns and Ni of the Hormone Sensitive Adenylyl Cyclase

Abstract

N_s and N_i have been purified without using NaF and Mg as stabilizing agents (Codina, J., Hildebrandt, J.D., Sekura, R.D., Birnbaumer, M., Bryan, J., Manclark, C.R. and Birnbaumer, L. [1984] J. Biol. Chem. 259, in press). Since the submission of that report, several modifications have been introduced to the purification procedure and additional fractions have been processed from which N proteins are obtained. This article describes the updated protocols and presents methodological details not included in the previous publication. The final products are N_s, the stimulatory N, N_i the inhibitory N, both of subunit structure αβγ, and a M_r=40,000 protein of βγ composition. They are obtained from human erythrocytes.     

Sulla presenza di micromiceti cellulosolitici in carta e cartone di varia provenienza

Riassunto L'A. si è interessato di ricercare in carta e cartoni da imballaggio provenienti da vari Paesi la presenza di microrganismi carticoli.Ha trovato che il 74% di campioni esaminati ospitano specie diChaetomium o di altri funghi (Aspergillus flavipes, Stachybotrys spp.,Memnoniella echinata, etc.) nonchè batteri cellulosololitici riferibili si generiCytophaga eCellvibrio.

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Summary The author has investigated microorganisms occurring on wrapping paper and cardboard. He found that 74% of the examined samples were containing species ofChaetomium and/or other fungi (Aspergillus flavipes, Stachybotrys spp.,Memnoniella echinata, etc.), as well as cellulosolytic Bacteria referable to generaCytophaga andCellvibrio.

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Sperimentatore presso il Laboratorio di Cartotecnica Speciale dell Ente Nazionale per la Cellulosa e per la Carta.     

Osservazioni ultrastrutturali sulle cellule di Leydig di Lacerta s. sicula Raf. in esemplari di gennaio e di maggio

Riassunto Gli autori descrivono gli aspetti ultrastrutturali delle cellule di Leydig di Lacerta s. sicula Raf`. in esemplari in letargo del mese di Gennaio (I gruppo) e in altri in periodo degli amori del mese di Maggio (II gruppo).Negli animali del primo gruppo le cellule di Leydig sono poco numerose, piccole, con scarso sviluppo del reticolo endoplasmatico, e mitocondri di dimensioni ridotte e prevalentemente con creste lamellari.Gli esemplari del secondo gruppo presentano cellule di Leydig numerose ed ipertrofiche con un R.E. liscio sviluppatissimo in forma di tubuli e di vescicole; i mitocondri sono numerosi, ipertrofici, con creste prevalentemente tubulari e con la parete spesso interrotta; sono presenti gocce lipidiche e vari lisosomi.La presenza dei caratteri ultrastrutturali propri delle cellule steroidogenetiche nelle cellule di Leydig degli esemplari di Lacerta s. sicula Raf`. in periodo degli amori, tende a confermare la loro partecipazione al metabolismo degli steroidi sessuali.

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On the fine structure of leydig cells in january and may specimens of Lacerta s. sicula Raf.

Summary In order to study the ultrastructure of the Leydig cells in the lizard Lacerta s. sicula Raf., the AA. examined two groups of animals, namely: January specimens in hibernation and May specimens in the mating period.In the animals of the first group, the Leydig cells were scarce, small and possessed a poorly developed E.R. and small mitochondria usually presenting laminar cristae.In the specimens of the second group the interstitial cells were large and possessed a very well developed smooth E.R. arranged in a system of anastomosing tubules and vesicles; the mitochondria were numerous and large, with prevailingly tubular cristae and often with a discontinuous wall. Lipid droplets and lysosomes were also present.The observation on the ultrastructure of the Leydig cells in May specimens of Lacerta s. sicula Raf`. seems to confirm the opinion that these elements participate in the metabolism of the sexual steroids; in fact they possess ultrastructural features that are typical of steroidogenetic cells.

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Lavoro eseguito con il contributo n.115.1121.1245. della Impresa di Endocrinologia del Consiglio Nazionale delle Ricerche, e svolto, per le osservazioni al M.E., presso il Centro di Studio di Microscopia Elettronica della Facoltà di Scienze dell'Università di Napoli.     

Il Gametofito Femmineo di Alcune Inuleae (Asteraceae)

Riassunto

È descritto lo sviluppo del gametofito secondo lo schema del « tipo normale » in Evax pygmaea Brot., Phagnalon rupestre DC., Helichrysum arenarium Moench., Inula Helenium L., Puliearia dysenterica Bernh. e ne è tratta occasione per una analisi delle fasi di vacuolizzazione e di polarizzazione. E considerato poi il valore sistematico dei caratteri del gametofito adulto.     

Embriologia Del “Buphthalmum Salicifolium„ L. (Asteraceae)

Riassunto

Sono descritte quattro forme morfologiche dello Sphenophyllum oblongifolium G. et K. della flora palcozoica del M. Pisano. E dimostrato una forte correlazione fra i seguenti caratteri: lunghezza dell'internodio, grado di anisofillia del verticillo, angolo di apertura fra le due paia di foglioline laterali sul verticillo stesso.     

Contributo All'Embriologia Delle Plumbaginaceae (con 103 figg. nel testo)

Riassunto

L'A. stabilisce che in Plumbago capensis Thunb., accanto allo sviluppo secondo lo schema del tipo Plumbago, si riscontra, con bassissima frequenza, un nuovo tipo di sviluppo di gametofiti 7-nucleati bipolarizzati con oosfera di natura sporiale.

Stabilisce inoltre che in Statice Limonium L. lo sviluppo del gametofito femminile avviene esclusivamente secondo il tipo Euphorbia dulcis, mentre in Armeria vulgaris W. var. maritima (Miller) Willd. esiste associazione dei due tipi di sviluppo Adoxa ed Euphorbia dulcis nel rapporto approssimativo del 3%.

Mette in evidenza che la triploidia dei nuclei calazali, caratteristica dei gametofiti di tipo Euphorbia dulcis, può determinarsi secondo due distinte modalità : o per coalescenza dei tre fusi calazali durante la terza divisione dello sviluppo (Statice Limonium), o per un processo di vera e propria cariogamia dei tre nuclei sporiali quiescenti durante lo stadio di polarizzazione 1+3 con conscguente formazione durante la storia dello sviluppo di uno stadio binucleato secondario (Armeria vulgaris var. maritima).

Riconferma infine per questa ultima pianta il numero aploide dei cromosomi n = 9.     

Saggi Sul Comportamento Della Cellula Vegetale di Fronte al Reattivo di Schiff

Riassunto

Sono state fatte diverse prove per la reazione di Schiff, di Feulgen e di Bauer su tessuti vegetali. Diversi tipi di membrane, fra cui quelle lignificate, sono risultati Schiff positivi. I nuclei sono risultati Feulgen positivi, con l'eccezione dei nuclei del gametofito maturo di Crinum sp., i quali sono tutti Feulgen negativi, meno quelli antipodali. L'amido e tutte le membrane sono risultati Bauer positivi.