共查询到20条相似文献,搜索用时 15 毫秒
1.
Andrei I Ivanov Stanislav N Samarin Moshe Bachar Charles A Parkos Asma Nusrat 《BMC cell biology》2009,10(1):36
Background
Disruption of epithelial cell-cell adhesions represents an early and important stage in tumor metastasis. This process can be modeled in vitro by exposing cells to chemical tumor promoters, phorbol esters and octylindolactam-V (OI-V), known to activate protein kinase C (PKC). However, molecular events mediating PKC-dependent disruption of epithelial cell-cell contact remain poorly understood. In the present study we investigate mechanisms by which PKC activation induces disassembly of tight junctions (TJs) and adherens junctions (AJs) in a model pancreatic epithelium. 相似文献2.
Background
In the Drosophila ovary, germ-line and soma cells are interconnected via gap junctions. The main gap-junction proteins in invertebrates are members of the innexin family. In order to reveal the role that innexins play in cell-cell communication during oogenesis, we investigated the localization of innexins 1, 2, 3 and 4 using immunohistochemistry, and analyzed follicle development following channel blockade. 相似文献3.
Alessandro Pintar Alfredo De Biasio Matija Popovic Neli Ivanova Sándor Pongor 《Biology direct》2007,2(1):19-13
The cytoplasmic tail of Notch ligands drives endocytosis, mediates association with proteins implicated in the organization of cell-cell junctions and, through regulated intra-membrane proteolysis, is released from the membrane as a signaling fragment. We survey these findings and discuss the role of Notch ligands intracellular region in bidirectional signaling and possibly in signal modulation in mammals. 相似文献
4.
Andrei I Ivanov Ingrid C McCall Brian Babbin Stanislav N Samarin Asma Nusrat Charles A Parkos 《BMC cell biology》2006,7(1):12-19
Background
Epithelial tight junction (TJ) and adherens junction (AJ) form the apical junctional complex (AJC) which regulates cell-cell adhesion, paracellular permeability and cell polarity. The AJC is anchored on cytoskeletal structures including actin microfilaments and microtubules. Such cytoskeletal interactions are thought to be important for the assembly and remodeling of apical junctions. In the present study, we investigated the role of microtubules in disassembly of the AJC in intestinal epithelial cells using a model of extracellular calcium depletion. 相似文献5.
Aartjan JW te Velthuis Jeroen F Admiraal Christoph P Bagowski 《BMC evolutionary biology》2007,7(1):129
Background
Development, differentiation and physiology of metazoans all depend on cell to cell communication and subsequent intracellular signal transduction. Often, these processes are orchestrated via sites of specialized cell-cell contact and involve receptors, adhesion molecules and scaffolding proteins. Several of these scaffolding proteins important for synaptic and cellular junctions belong to the large family of membrane-associated guanylate kinases (MAGUK). In order to elucidate the origin and the evolutionary history of the MAGUKs we investigated full-length cDNA, EST and genomic sequences of species in major phyla. 相似文献6.
Background
Adherens junctions consist of transmembrane cadherins, which interact intracellularly with p120ctn, ß-catenin and α-catenin. p120ctn is known to regulate cell-cell adhesion by increasing cadherin stability, but the effects of other adherens junction components on cell-cell adhesion have not been compared with that of p120ctn.Methodology/Principal Findings
We show that depletion of p120ctn by small interfering RNA (siRNA) in DU145 prostate cancer and MCF10A breast epithelial cells reduces the expression levels of the adherens junction proteins, E-cadherin, P-cadherin, ß-catenin and α-catenin, and induces loss of cell-cell adhesion. p120ctn-depleted cells also have increased migration speed and invasion, which correlates with increased Rap1 but not Rac1 or RhoA activity. Downregulation of P-cadherin, β-catenin and α-catenin but not E-cadherin induces a loss of cell-cell adhesion, increased migration and enhanced invasion similar to p120ctn depletion. However, only p120ctn depletion leads to a decrease in the levels of other adherens junction proteins.Conclusions/Significance
Our data indicate that P-cadherin but not E-cadherin is important for maintaining adherens junctions in DU145 and MCF10A cells, and that depletion of any of the cadherin-associated proteins, p120ctn, ß-catenin or α-catenin, is sufficient to disrupt adherens junctions in DU145 cells and increase migration and cancer cell invasion. 相似文献7.
8.
Background
Tight junctions are an intercellular adhesion complex of epithelial and endothelial cells, and form a paracellular barrier that restricts the diffusion of solutes on the basis of size and charge. Tight junctions are formed by multiprotein complexes containing cytosolic and transmembrane proteins. How these components work together to form functional tight junctions is still not well understood and will require a complete understanding of the molecular composition of the junction. 相似文献9.
Ramray Bhat Kenneth M Lerea Hong Peng Herbert Kaltner Hans-Joachim Gabius Stuart A Newman 《BMC developmental biology》2011,11(1):6
Background
The skeletal elements of vertebrate embryonic limbs are prefigured by rod- and spot-like condensations of precartilage mesenchymal cells. The formation of these condensations depends on cell-matrix and cell-cell interactions, but how they are initiated and patterned is as yet unresolved. 相似文献10.
Vishaldeep K Sidhu Frank-Jörg Vorhölter Karsten Niehaus Steven A Watt 《BMC microbiology》2008,8(1):87
Background
Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic E. coli. 相似文献11.
Background
Scaffolding proteins belonging to the membrane associated guanylate kinase (MAGUK) superfamily function as adapters linking cytoplasmic and cell surface proteins to the cytoskeleton to regulate cell-cell adhesion, cell-cell communication and signal transduction. We characterize here a Drosophila MAGUK member, Varicose (Vari), the homologue of vertebrate scaffolding protein PALS2. 相似文献12.
Background
A complex of three cell adhesion molecules (CAMs) Neurexin IV(Nrx IV), Contactin (Cont) and Neuroglian (Nrg) is implicated in the formation of septate junctions between epithelial cells in Drosophila. These CAMs are interdependent for their localization at septate junctions and e.g. null mutation of nrx IV or cont induces the mislocalization of Nrg to the baso-lateral membrane. These mutations also result in ultrastructural alteration of the strands of septate junctions and breakdown of the paracellular barrier. Varicose (Vari) and Coracle (Cora), that both interact with the cytoplasmic tail of Nrx IV, are scaffolding molecules required for the formation of septate junctions. 相似文献13.
Xiao-Kui Ma Li Wang Yu Li Xiang-Ming Yang Pu Zhao HaoTang Ping Zhu Ling Li Zhi-Nan Chen 《BMC cell biology》2010,11(1):27
Background
Acquisition of resistance to "anoikis" facilitates the survival of cells under independent matrix-deficient conditions, such as cells in tumor progression and the production of suspension culture cells for biomedical engineering. There is evidence suggesting that CD147, an adhesion molecule associated with survival of cells in tumor metastasis and cell-cell contacts, plays an important role in resistance to anoikis. However, information regarding the functions of CD147 in mediating cell-cell contacts and anoikis-resistance remains limited and even self-contradictory. 相似文献14.
Martin Gruene Claudia Unger Lothar Koch Andrea Deiwick Boris Chichkov 《Biomedical engineering online》2011,10(1):19
Background
Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities. 相似文献15.
Guo-Feng Ma Simo Miettinen Pauliina Porola Klaus Hedman Jari Salo Yrjö T Konttinen 《BMC microbiology》2009,9(1):55-7
Background
The aim of the study was to investigate expression of ADAMs (A Disintegrin and A Metalloproteinase) of host cell origin during cell-cell fusion induced by human parainfluenza virus type 2 (HPIV2). 相似文献16.
Mallory R Frederick Christina Kuttler Burkhard A Hense Hermann J Eberl 《Theoretical biology & medical modelling》2011,8(1):8
Background
Biofilms are microbial communities encased in a layer of extracellular polymeric substances (EPS). The EPS matrix provides several functional purposes for the biofilm, such as protecting bacteria from environmental stresses, and providing mechanical stability. Quorum sensing is a cell-cell communication mechanism used by several bacterial taxa to coordinate gene expression and behaviour in groups, based on population densities. 相似文献17.
A strategy for enrichment of claudins based on their affinity to Clostridium perfringens enterotoxin
D?rte Lohrberg Eberhard Krause Michael Schümann J?rg Piontek Lars Winkler Ingolf E Blasig Reiner F Haseloff 《BMC molecular biology》2009,10(1):61
Background
Claudins, a family of protein localized in tight junctions, are essential for the control of paracellular permeation in epithelia and endothelia. The interaction of several claudins with Clostridium perfringens enterotoxin (CPE) has been exploited for an affinity-based enrichment of CPE-binding claudins from lysates of normal rat cholangiocytes. 相似文献18.
Birendra Mishra Keiichiro Kizaki Katsuo Koshi Koichi Ushizawa Toru Takahashi Misa Hosoe Takashi Sato Akira Ito Kazuyoshi Hashizume 《Reproductive biology and endocrinology : RB&E》2010,8(1):60
Background
Extracellular matrix metalloproteinase inducer (EMMPRIN) regulates several biological functions involving the modulation of cell behaviors via cell-cell and cell-matrix interactions. According to its diverse functions, we hypothesized that EMMPRIN may play an important role in endometrial remodeling and establishment of pregnancy in cow. 相似文献19.
Neal E Beeman Heidi K Baumgartner Patricia G Webb Jerome B Schaack Margaret C Neville 《BMC cell biology》2009,10(1):85
Background
Occludin is a tetraspanin protein normally localized to tight junctions. The protein interacts with a variety of pathogens including viruses and bacteria, an interaction that sometimes leads to its extrajunctional localization. 相似文献20.
Lasse Evensen David R. Micklem Anna Blois Sissel Vik Berge Niels Aars?ther Amanda Littlewood-Evans Jeanette Wood James B. Lorens 《PloS one》2009,4(6)