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1.
E. I. Samarova V. V. Zhukov K. A. Sudoplatov 《Journal of Evolutionary Biochemistry and Physiology》2005,41(2):169-175
The study was performed on effects of serotonin and its antagonists (mianserin, propranolol, and metergoline) on efferent electrical activity in nerves cervicalis superior, cervicalis inferior, and columellaris innervating muscles withdrawing body of Lymnaea stagnalis into the shell. Serotonin had a dual effect on the off-reactions caused by rhythmical light stimulation of mollusc skin. The number of responses to series of stimuli increased at serotonin concentrations of about 10−8-10−7 M and decreased at its higher concentrations. In many cases, serotonin antagonists also had a dual effect depending on their concentration. All studied substances slightly affected duration and latent period of individual off-responses. Serotoninergic regulation is suggested to participate in central chains of the pond snail defensive shadow reflex.__________Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 2, 2005, pp. 134–138.Original Russian Text Copyright © 2005 by Samarova, Zhukov, Sudoplatov. 相似文献
2.
Responses of rabbit visual cortical neurons to single and repetitive intracortical electrical stimulation were investigated. The stimulating electrode was located 0.7–1.2 mm away from the recording electrode. Response thresholds to single stimulation were as a rule 150–180 µA, whereas to series of stimuli they were 30–60 µA. The latent period to the first spike averaged 5–15 msec but the probability of the initial discharge was very low, namely 3–6%. With an increase in current intensity the duration of the initial inhibitory pause was increased in half of the neurons responding to it, whereas in the rest it was unchanged. After presentation of series of stimuli spontaneous activity was enhanced for a short time (4–6 sec). In about half of the cells the same kinds of discharge dynamics were observed in response to repetitive stimulation (frequency 0.25 Hz) as in responses to light, but more neurons with sensitization of discharge and fewer "habituating" neurons took part in responses to electrical stimulation. It is postulated that stimulation of a given point of the visual cortex evokes excitation of a local neuron hypercolumn and inhibition of neighboring cell columns.M. V. Lomonosov Moscow State University. Translated from Neirofiziologiya, Vol. 15, No. 4, pp. 412–419, July–August, 1983. 相似文献
3.
Yu. I. Arshavskii M. B. Berkinblit L. B. Popova O. I. Fukson V. S. Yakobson 《Neurophysiology》1979,11(6):438-444
The effect of the central analgesic fentanyl on evoked potentials and responses of single Purkinje cells in the cerebellar cortex to stimulation of the sensomotor cortex and of somatic nerves was investigated in unanesthetized cats. Injection of fentanyl in an analgesic dose (10–30 µg/kg, intravenously) had no appreciable effect on evoked potentials and led only to very slight changes in spontaneous activity and responses of Purkinje cells. As a rule fentanyl, in a dose of 30 µg/kg, depressed, but in a dose of 10 µg/kg, facilitated responses and spontaneous activity of Purkinje cells. This effect of fentanyl is negligible compared with the action of barbiturate anesthetics on responses of cerebellar cortical neurons. Consequently, fentanyl can be used for analgesia during electrophysiological investigations of the cerebellar cortex in unanesthetized cats.Institute for Problems in Information Transmission, Academy of Sciences of the USSR, Moscow. M. V. Lomonosov Moscow State University. Translated from Neirofiziologiya, Vol. 11, No. 6, pp. 585–592, November–December, 1979. 相似文献
4.
Activity of 112 neurons of the precruciate motor cortex in cats was studied during a forelimb placing reaction to tactile stimulation of its distal parts. The latent period of response of the limb to tactile stimulation was: for flexors of the elbow (biceps brachii) 30–40 msec, for the earliest reponses of cortical motor neurons about 20 msec. The biceps response was observed 5–10 msec after the end of stimulation of the cortex with a series of pulses lasting 25 msec. Two types of excitatory responses of the neurons were identified: responses of sensory type observed to each tactile stimulation of the limb and independent of the presence or absence of motion, and responses of motor type, which developed parallel with the motor response of the limb and were not observed in the absence of motion. The minimal latent period of the responses of motor type was equal to the latent period of the sensory responses to tactile stimulation (20±10 msec). Stimulation of the cortex through the recording microelectrode at the site of derivation of unit activity, which increased during active flexion of the forelimb at the elbow (11 stimuli at intervals of 2.5 msec, current not exceeding 25 µA), in 70% of cases evoked an electrical response in the flexor muscle of the elbow.M. V. Lomonosov Moscow State University. Translated from Neirofiziologiya, Vol. 9, No. 2, pp. 115–123, March–April, 1977. 相似文献
5.
Y. Hosokawa J. Horikawa M. Nasu I. Taniguchi 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,181(6):607-614
Spatio-temporal patterns of binaural interaction in the guinea pig auditory cortex (AC) were observed using optical recording
with a 12 × 12 photodiode array and a voltage-sensitive dye. The amplitudes of the sound-induced light signals from the cortex
were transformed into sequential two-dimensional images every 0.58 ms. Binaural sound stimuli evoked an excitatory response
followed by a strong inhibition, and contralateral stimuli evoked a strong excitatory response followed by a weak inhibition.
Ipsilateral sound stimuli evoked a weak response. Binaural stimulation induced two types of ipsilateral inhibition: a fast
binaural inhibition which was detected only after the contralateral and ipsilateral responses were subtracted from the binaural
responses, and which appeared 12–25 ms after the onset of stimulation, and a slow binaural inhibitory effect which was clearly
observed in the binaural responses themselves, appearing 70–95 ms after the onset of stimulation. The fast binaural inhibition
was observed in the same area as the contralateral excitatory response. The inhibited area became stronger and more widespread
with increasing intensity of ipsilateral stimulation. We did not observe the specialized organization of binaural neurons
as electrophysiologically found in the cat AC, in which binaural neurons of the same binaural response type are clustered
together and alternate with clusters of other response types.
Accepted: 14 August 1997 相似文献
6.
Combined presentation of food and noxious electrical stimulation produced no response in snails injected 6–16 days previously with 5,7-dihydrooxytryptamine, which produces degeneration of serotoninergic nerve terminals and reduced serotonin synthesis, although a defense (aversive) response was observed in the control group. Application of serotonin to a preparation of the snail central nervous system (contained in a bath) was used as reinforcement during neurophysiological experiments. The amplitude of synaptic response to nerve stimulation increased significantly in preparations in which stimulation was paired with serotonin application. After 3–7 sets of twin stimuli consisting of serotonin application and applying a drop of juice to the chemoreceptive surface area of the skin, a spike response to the latter stimulus was produced. No such effects were seen in response to unpaired stimuli. It was deduced that serotonin makes a major contribution to establishing conditioned aversive reactions in the snail.Institute of Higher Nervous Activity and Neurophysiology, Academy of Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 18, No. 3, pp. 291–298, May–June, 1986. 相似文献
7.
Single unit responses in the visual cortex of the waking rabbit to repetitive photic stimulation at a frequency of once every 2.5 sec were studied. Depending on the total number of spikes in the response, the dynamics of the responses could be divided into two types: "fast" and "slow." From 5 to 15 stimuli were required to establish a stable level of response with changes of the first type, but 50 to 100 stimuli were needed for the response with changes of the second type. About 50% of all neurons did not change the characteristics of response. In the group of neurons with changing responses, partial habituation was found in 55–59% of cells; there were 25% of neurons with sensitization of discharge and 17–20% with a humpbacked type of response dynamics. A "slow" dynamics of unit responses in most cases was accompanied by changes in the duration of inhibitory pauses in the response; negative correlation of these values was observed in 65% of neurons.M. V. Lomonosov Moscow State University. Translated from Neirofiziologiya, Vol. 10, No. 5, pp. 451–459, September–October, 1978. 相似文献
8.
Neuronal firing response in the sensorimotor cortex to tactile (non-conditioned) and acoustic (conditioned) stimuli was investigated in trained cats before and after iontophoretic application of serotonin and lysergide. Three functionally distinct groups of neurons were identified from the response produced by presenting tactile and acoustic stimuli. Applying serotonin was found to facilitate preliminary and residual spike response induced by tactile stimulation; it also facilitates and modulates response in many cortical neurons to conditioned stimuli. Facilitation takes the form of reduced latency of response and increased numbers of spikes in response to conditioned stimulus presentation, especially at the initial phase of response to sound and immediately after the onset of conditioned reflex motion. Additional neurons formerly unresponsive to acoustic stimuli joined in the reaction under the effects of serotonin. Changed response patterns often evolve following minor fluctuations in background activity level. It is suggested that facilitation of response following iontophoretic serotonin application in the neocortex is associated with activation of excitatory serotonin receptors (S2). The lysergide-induced increase in background and evoked activity noted during experimentation can apparently be put down to blockade of inhibitory serotonon (S1B) receptors.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 22, No. 3, pp. 337–347, May–June, 1990. 相似文献
9.
Summary Cultured epithelial cells (Intestine 407) derived from fetal human small intestine exhibited spontaneous oscillations of membrane potential between the resting level of about –20 mV and the activated level of about –75mV. The cells were hyperpolarized to the latter level in response to mechanical or electrical stimuli. The hyperpolarizing responses were also elicited by the application of intestinal secretagogues: acetylcholine, histamine, serotonin and vasoactive intestinal polypeptide (VIP). The spontaneous oscillation of membrane potential became prominent and long-lasting in the presence of acetylcholine, histamine, serotonin or VIP. These secretagogue-induced responses were mediated by individual independent receptors on the cell membrane. Muscarinic receptors were responsible for the acetylcholine response, and H1-receptors for the histamine response. The cells also responded with a slow hyperpolarization to calcium ionophore A23187, which is known to induce intestinal secretion. The spontaneously occurring hyperpolarizing responses and those induced by stimuli were both due to an increase in the K+ conductance of the cell membrane. Since acetylcholine, histamine, serotonin and A23187 are known to promote mobilization of cellular Ca2+ ions in intestinal secretory cells, it is hypothesized that these electrical activities of the cell are closely related to the receptor stimulation which leads to the Ca2+-mediated intestinal secretion. 相似文献
10.
Ancient DNA analysis identifies marine mollusc shells as new metagenomic archives of the past
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Clio Der Sarkissian Vianney Pichereau Catherine Dupont Peter C. Ilsøe Mickael Perrigault Paul Butler Laurent Chauvaud Jón Eiríksson James Scourse Christine Paillard Ludovic Orlando 《Molecular ecology resources》2017,17(5):835-853
Marine mollusc shells enclose a wealth of information on coastal organisms and their environment. Their life history traits as well as (palaeo‐) environmental conditions, including temperature, food availability, salinity and pollution, can be traced through the analysis of their shell (micro‐) structure and biogeochemical composition. Adding to this list, the DNA entrapped in shell carbonate biominerals potentially offers a novel and complementary proxy both for reconstructing palaeoenvironments and tracking mollusc evolutionary trajectories. Here, we assess this potential by applying DNA extraction, high‐throughput shotgun DNA sequencing and metagenomic analyses to marine mollusc shells spanning the last ~7,000 years. We report successful DNA extraction from shells, including a variety of ancient specimens, and find that DNA recovery is highly dependent on their biomineral structure, carbonate layer preservation and disease state. We demonstrate positive taxonomic identification of mollusc species using a combination of mitochondrial DNA genomes, barcodes, genome‐scale data and metagenomic approaches. We also find shell biominerals to contain a diversity of microbial DNA from the marine environment. Finally, we reconstruct genomic sequences of organisms closely related to the Vibrio tapetis bacteria from Manila clam shells previously diagnosed with Brown Ring Disease. Our results reveal marine mollusc shells as novel genetic archives of the past, which opens new perspectives in ancient DNA research, with the potential to reconstruct the evolutionary history of molluscs, microbial communities and pathogens in the face of environmental changes. Other future applications include conservation of endangered mollusc species and aquaculture management. 相似文献
11.
We compared gastric acid secretion in response to various stimuli in normal and streptozotocin (STZ)-induced diabetic rats, in an attempt to characterize the alteration of acid secretory response in diabetic conditions. Animals were injected STZ (70 mg x kg(-1), i.p.) and used after 5 weeks of diabetes with blood glucose > 350 mg x dL(-1). Under urethane anesthesia, a rat stomach was mounted on an ex vivo chamber, perfused with saline and acid secretion was measured at pH 7.0 using a pH-stat method and by adding 100 mM NaOH. The acid secretion was stimulated by i.v. infusion of either histamine (4 mg x kg(-1) x h(-1)), pentagastrin (60 microg x kg(-1) x h(-1)) or carbachol (20 microg x kg(-1) x h(-1)) or i.v. injection of YM-14673 (0.3 mg x kg(-1)), an analog of thyrotropin-releasing hormone, or vagal electrical stimulation (2 ms, 3 Hz, 0.5 mA). In normal rats, gastric acid secretion was increased in response to either histamine, pentagastrin, carbachol, YM-14673 or electrical vagal stimulation. In STZ diabetic rats, however, changes in acid secretion varied depending on the stimuli; the acid secretory responses to histamine remained unchanged, those to YM-14673 and vagal electrical stimulation significantly decreased, but the responses to both pentagastrin and carbachol were significantly enhanced as compared to normal rats. Luminal release of histamine in response to both pentagastrin and carbachol was increased in STZ-diabetic rats as compared to normal animals. The altered acid secretory responses in STZ diabetic rats were partially reversed by daily injection of insulin with amelioration of high blood glucose levels. These results suggest that STZ-diabetic rats showed different changes in gastric acid secretory responses to various stimuli; no change in response to histamine, a decrease to both YM-14673 and vagal electrical stimulation and an increase to both pentagastrin and carbachol. The increased acid secretory response may be associated with an enhanced release of mucosal histamine, while the decreased response may be due to vagal neuropathy. 相似文献
12.
N. A. Otmakhov 《Neurophysiology》1982,14(4):309-316
The effect of noradrenalin (NA) and serotonin (5-HT) on responses of area CA3 cells evoked by electrical stimulation of mossy fibers was studied in slices of guinea pig hippocampus survivingin vitro. Both substances, which modify the general level and organization of spontaneous activity, also affected responses of area CA3 cells. Changes in magnitude and structure of the response usually correlated with corresponding changes in spontaneous activity. In certain cases NA, which lowered the frequency of spontaneous activity but increased its relative content of "complex discharges" and also the number of reduced action potentials in the complex discharge, also led to an increase in the response to stimulation. 5-HT evoked periodic grouped activity in some cells and led to the appearance of such grouped discharges for the first time in the responses of other cells. Unlike NA, 5-HT caused prolonged (up to 40 min) after-facilitation of the response and an increase in spontaneous discharge frequency.Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino-on-Oka. Translated from Neirofiziologiya, Vol. 14, No. 4, pp. 410–417, July–August, 1982. 相似文献
13.
《Electronic Notes in Theoretical Computer Science》1986,65(3):231-235
Endogenous components of evoked potentials resembling P300 in humans were sequentially studied in 3 cynomolgus monkeys (Macaca fascicularis) using an auditory ‘oddball’ paradigm. The two different auditory stimuli were 500 Hz and 4000 Hz tones, designated as the ‘frequent’ and ‘rare’ stimuli, respectively. The probability of ‘rare’ tone presentation was initially 0.2. We further used probabilities of 0.1, 0.3 and 0.5. The ‘rare’ stimulus was reinforced by electrical stimulation, which followed the onset of the high tone by 700 msec. After 3–5 training sessions, a late positive wave was observed following the ‘rare’ tone. The latency of this P300-like signal was 314±16.2 msec, and teh amplitude 23.6±3.14 μV. The amplitude of this potential was modified by changes in stimulus presentation probability and by withholding reinforcement. 相似文献
14.
Spontaneous activity of frontal cortical neurons (middle part of sulcus principalis) and their responses to stimuli of different biological significance were studied in alert monkeys. The region studied is characterized by a lower level of spontaneous activity than the motor cortex. Fluctuations in the level of spontaneous activity are connected with changes in the experimental situation. Two types of unit responses were distinguished to repetitive stimulation: preservation of responses and habituation. The type of unit response depends on the relationship of the stimuli to the animal's motivational sphere.A. A. Ukhtomskii Physiological Research Institute, A. A. Zhdanov Leningrad State University. Translated from Neirofiziologiya, Vol. 12, No. 1, pp. 3–12, January–February, 1980. 相似文献
15.
A. V. Sidorov 《Journal of Evolutionary Biochemistry and Physiology》2006,42(1):43-48
The correlation was studied between the hemolymph pH value and the locomotion rate in the mollusc Lymnaea stagnalis at different degree of activation of the food-procuring behavior. It is established that the hemolymph pH value remains unchanged in the satiated (hungry), freely moving Lymnaea individuals, and, hence, it does not determine the locomotor activity of molluscs in these functional states. In the course of food consumption, locomotion decreases significantly, which is due to an observed decrease of the hemolymph pH. This effect is realized through the corresponding changes of electrical activity of motoneurons of the locomotor network of the Lymnaea nervous system (PeA-cluster). It is suggested that the pH value plays the modulating role in coordination of activities of the nervous centers of the mollusc nutrition and locomotion. 相似文献
16.
17.
Synaptic and spike responses of neurons were studied in a two-layered model based on a study of the structure of the neuronal net of the defensive reflex in snails [4], using a technique of simultaneous application of two equal stimuli. When the points of application of the two stimuli were brought closer together the bimodal distribution of synaptic and spike responses of the efferent neurons of the model gradually changed into unimodal. The character of quantitative changes in synaptic and spike responses of neurons of the model, as the points of application of the two stimuli move closer together, reflects well the character of changes in effector responses of the snail's foot under experimental conditions as the distance between the two simultaneously acting tactile stimuli is reduced. After removal of the CNS, responses of contraction of the foot muscles of the snail become less accurate (they have a more diffuse maximum). It is suggested that the difference between functions of the central and peripheral nerve nets in molluscs lies in differences in the degree of accuracy of performance of the reflex response.Institute of Higher Nervous Activity and Neurophysiology, Academy of Sciences of the USSR, Moscow. Translated from Neirofiziologiya, Vol. 15, No. 6, pp. 604–610, November–December, 1983. 相似文献
18.
Experiments on unanesthetized, immobilized cats showed different effects of individual hypothalamic nuclei on spontaneous unit activity in the sensomotor cortex. Compared with the posterior hypothalamic nucleus (PHN) and its anteromedial region (AMH), in response to stimulation of the lateral hypothalamic nucleus (LHN) changes in spontaneous activity were more frequently found. The ratio between activation and inhibitory responses was 36 and 36% for AMH, 51 and 30% for LHN, and 47 and 28% for PHN. An approximately equal number of sensomotor neurons (27–34%) gave tonic responses. Phasic changes in spontaneous activity were observed more often in response to stimulation of LHN, less frequently to stimulation of AMH and PHN. Responses of "nonpyramidal" neurons to stimulation of AMH and LHN were identical. "Pyramidal" units were more sensitive to LHN stimulation. Variations in hypothalamic effects depending on the type of response of sensomotor neurons to sensory stimuli were detected. Cells with tonic responses were more susceptible to influences of LHN and AMH than cells which responded by phasic changes in spontaneous activity to sensory stimuli. Fewer still positive responses were recorded in areactive neurons.Medical Institute, Chita. Translated from Neirofiziologiya, Vol. 4, No. 2, pp. 115–122, March–April, 1972. 相似文献
19.
Preliminary investigations on the occurrence of molluscs and waterfowl at the man-made Lake Zegrzyskie were begun in 1986.The numbers, biomass and dominance structure of molluscs were analysed at different stations in the Lake. Some mollusc species were observed in huge numbers. Waterfowl, especially benthivorous species, were found in big flocks all the year round, but reached highest numbers in autumn. The possible effects of predation pressure from waterfowl on mollusc communities were analysed. 相似文献
20.
Leslie D. Manley Ronald Kuczenski David S. Segal Stephen J. Young Philip M. Groves 《Journal of neurochemistry》1992,58(4):1491-1498
In vivo microdialysis was employed to detect changes in extracellular dopamine and serotonin in the rat caudate in response to electrical stimulation of the medial forebrain bundle. Extracellular dopamine concentrations increased linearly as a function of the frequency (4-33 Hz) of evenly spaced stimuli in both the presence and absence of cocaine added to the dialysate. Because dopamine neurons are known to fire in single-spike and burst patterns, stimulation pulses were also delivered in a bursting pattern. The response of extracellular dopamine was augmented in both the presence and absence of cocaine when the same number of stimuli were delivered in bursts as compared to an evenly spaced pattern. Serotonin, which was only assessed in the presence of cocaine, similarly increased linearly with frequency, but, in contrast to the dopamine response, levels of serotonin were not augmented by stimuli presented in bursts. These results suggest that microdialysis can be used to detect physiological changes in synaptic transmitter concentrations. 相似文献